VIP Made in USA — Third-Party Verified
Vasoactive Intestinal Peptide (VIP) is a heavily studied 28-amino acid regulatory neuropeptide implicated in neuroendocrine signaling, immune homeostasis, and vascular dynamics. PX1 Research delivers domestically synthesized VIP produced under strict quality control standards to ensure consistent, highly reproducible experimental outcomes. Every lot undergoes independent third-party testing at an ISO 17025 accredited laboratory, complete with HPLC purity verification and mass spectrometry characterization.
Quick answer
Vasoactive Intestinal Peptide (VIP) is a heavily studied 28-amino acid regulatory neuropeptide implicated in neuroendocrine signaling, immune homeostasis, and vascular dynamics. PX1 Research delivers domestically synthesized VIP produced under strict quality control standards to ensure consistent, highly reproducible experimental outcomes. Every lot undergoes independent third-party testing at an ISO 17025 accredited laboratory, complete with HPLC purity verification and mass spectrometry characterization.
Key takeaways
- Vasoactive Intestinal Peptide (VIP) is an endogenous 28-amino acid peptide belonging to the glucagon/secretin superfamily of regulatory peptides.
- The primary structure of VIP comprises a single non-glycosylated polypeptide chain with the sequence His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2.
- In preclinical model systems, VIP acts as a high-affinity ligand for two class B G-protein-coupled receptors: VPAC1 and VPAC2.
- To guarantee that every vial of VIP meets strict scientific requirements, PX1 Research subjects all production batches to independent third-party verification.
Introduction to Vasoactive Intestinal Peptide (VIP) in Preclinical Research
Vasoactive Intestinal Peptide (VIP) is an endogenous 28-amino acid peptide belonging to the glucagon/secretin superfamily of regulatory peptides. Discovered initially in the gastrointestinal tract, subsequent preclinical investigations revealed its wide distribution throughout the central nervous system, peripheral nerve fibers, and immune tissues. In laboratory settings, researchers utilize VIP research compounds to explore fundamental signal transduction pathways, particularly those governing smooth muscle relaxation, epithelial secretion, and neurogenic inflammation modulation.
Because regulatory neuropeptide pathways intersect with multiple physiological cascades, acquiring high-purity material is essential for maintaining experimental validity. Minor contaminants, sequence truncations, or residual synthesis reagents can confound cell culture assays and receptor-binding studies. Researchers exploring the broader landscape of neuropeptide signaling can consult our comprehensive research library for comparative literature and biochemical references.
Chemical Structure and Domestic Synthesis Parameters
The primary structure of VIP comprises a single non-glycosylated polypeptide chain with the sequence His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2. The C-terminal amidation is a critical structural feature required for full biological activity and receptor binding affinity in vitro. Sourcing high-grade vip made in usa ensures that solid-phase peptide synthesis (SPPS) parameters—such as coupling efficiency, cleavage conditions, and side-chain deprotection—are rigorously optimized.
Domestic manufacturing in cGMP-compliant facilities eliminates the supply chain uncertainties and quality variability often associated with unverified overseas producers. By executing synthesis under controlled parameters in the USA, PX1 Research prevents sequence micro-heterogeneity, racemic amino acid incorporation, and oxidation of sensitive residues like Methionine-17. This precision yields a stable, highly defined lyophilized powder ideal for precise quantitative biochemical assays.
Receptor Targets and In Vitro Signaling Dynamics
In preclinical model systems, VIP acts as a high-affinity ligand for two class B G-protein-coupled receptors: VPAC1 and VPAC2. Both receptors activate adenylate cyclase upon ligand binding, precipitating an intracellular surge of cyclic adenosine monophosphate (cAMP) and subsequent activation of Protein Kinase A (PKA). Preclinical data indicate that this signaling cascade negatively regulates nuclear factor kappa B (NF-κB) nuclear translocation, resulting in down-regulation of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-12 in cultured macrophages and microglia.
Additionally, VIP exhibits cross-reactivity at lower affinities with the PAC1 receptor, a target primarily bound by Pituitary Adenylate Cyclase-Activating Polypeptide. Investigators analyzing immunomodulatory networks often evaluate VIP alongside other regulatory molecules like Thymosin Alpha-1 to observe differential impacts on T-cell maturation, dendritic cell suppression, and anti-inflammatory signaling cascades in isolated primary cell culture systems.
Analytical Rigor: HPLC, Mass Spectrometry, and ISO 17025 Verification
To guarantee that every vial of VIP meets strict scientific requirements, PX1 Research subjects all production batches to independent third-party verification. Testing is performed in an ISO 17025 accredited laboratory utilizing high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). High-performance liquid chromatography measures chemical purity, ensuring that target peptide content consistently meets or exceeds 98% purity, free from truncated sequences or chemical adducts.
Mass spectrometry confirms the exact molecular weight (3325.8 Da) of the synthetic construct, verifying correct amino acid composition and total sequence fidelity. Furthermore, because VIP is frequently introduced to sensitive cell lines, every lot undergoes chromogenic Limulus Amebocyte Lysate (LAL) testing to confirm low bacterial endotoxin content (<0.05 EU/mg). A batch-specific Certificate of Analysis (COA) detailing these metrics is published openly for every order.
Comparative Analysis: VIP vs. Related Regulatory Neuropeptides
When designing comparative in vitro or animal models, researchers frequently evaluate VIP alongside structural and functional analogs within the neuroendocrine and immunomodulatory peptide classes. Understanding the distinct receptor profiles and metabolic stabilities across these compounds helps investigators refine experimental controls.
While VIP targets VPAC1 and VPAC2 to stimulate cAMP production and suppress pro-inflammatory transcription factors, PACAP-38 exhibits high-affinity binding across VPAC1, VPAC2, and the dedicated PAC1 receptor, making it a distinct probe for neuroprotective research. In contrast, host-defense peptides like LL-37 modulate innate immune responses via direct membrane interaction and chemokine receptor activation rather than class B GPCR pathways. Similarly, tissue-repair peptides such as BPC-157 operate through angiogenic growth factor modulation and nitric oxide signaling pathways rather than primary VPAC signaling. Evaluating these complementary mechanisms allows researchers to dissect distinct cellular communication channels.
Reconstitution Protocols and Solvent Compatibility for Lab Use
Lyophilized VIP requires proper reconstitution protocols to maintain structural integrity and prevent aggregation or surface adsorption. For standard in vitro assays, lyophilized VIP should be reconstituted using sterile, non-pyrogenic diluents such as bacteriostatic water or sterile phosphate-buffered saline (PBS, pH 7.4). Because VIP contains basic amino acid residues (Lysine, Arginine), initial solubilization in neutral to slightly acidic aqueous buffer promotes optimal dissolution.
To prevent non-specific binding of the peptide to glass or plastic container walls at low working concentrations (e.g., <100 nM), researchers frequently supplement working buffers with 0.1% carrier protein, such as Bovine Serum Albumin (BSA) or human serum albumin. Rapid vortexing should be strictly avoided; gentle agitation or gentle inversion is recommended to dissolve the lyophilized cake without introducing shear stress or foaming.
Thermal Stability, Aliquoting, and Handling Guidelines
Lyophilized VIP demonstrates superior long-term stability when stored at -20°C to -80°C in a desiccated environment protected from direct light exposure. Prior to opening, vials should be allowed to equilibrate to room temperature to prevent atmospheric moisture condensation inside the container, which can accelerate peptide hydrolysis over time.
Once reconstituted into aqueous solution, VIP should be aliquoted into single-use microcentrifuge tubes to prevent repeated freeze-thaw cycles, which degrade peptide bond integrity and promote methionine oxidation. Reconstituted aqueous solutions stored at 4°C should generally be utilized within 24 to 48 hours, whereas stock aliquots stored at -80°C remain stable for extended research periods. Detailed technical protocols for peptide handling are accessible via our research hub.
USA Manufacturing vs. Imported Research Compounds
The market for laboratory research peptides contains significant variance in product quality, driven largely by foreign manufacturers utilizing unverified synthesis facilities and lacking analytical transparency. Foreign imports frequently present issue vectors such as batch-to-batch inconsistency, residual heavy metals, TFA salt contamination, and high endotoxin levels that can invalidate sensitive cellular assays.
Sourcing vip made in usa guarantees direct oversight, fully traceable chemical precursors, and adherence to domestic analytical standards. PX1 Research operates domestic manufacturing and distribution nodes out of California and Arizona, allowing fast, reliable fulfillment without international customs delays. Institutional buyers seeking verified materials can streamline supply management through our dedicated wholesale program.
Procurement and Institutional Quality Assurance at PX1 Research
PX1 Research is committed to supplying universities, biotechnology firms, and independent research institutions with pure, rigorously characterized research compounds. We ship all orders directly from our facilities in California and Arizona, offering same-day dispatch for orders placed before 3:00 PM PST, Monday through Friday.
By enforcing mandatory HPLC and ESI-MS lot verification alongside endotoxin screening, PX1 Research provides verified reagents designed to satisfy strict institutional compliance requirements. Researchers investigating growth factor secretagogues like CJC-1295 or neuropeptide regulators can trust PX1 for transparent analytical documentation and reliable domestic supply.
Frequently Asked Questions
What is the purity level of VIP supplied by PX1 Research?
Every batch of VIP supplied by PX1 Research is verified by high-performance liquid chromatography (HPLC) to meet or exceed 98% chemical purity. Complete analytical data is provided on the lot-specific Certificate of Analysis.
Where is PX1 Research VIP synthesized and dispatched from?
PX1 Research VIP is synthesized domestically in the USA within cGMP-compliant facilities. Orders are fulfilled directly from our domestic distribution centers located in California and Arizona.
What analytical testing is performed on each lot of VIP?
Each lot undergoes reverse-phase HPLC for purity determination, Electrospray Ionization Mass Spectrometry (ESI-MS) for molecular weight confirmation, and chromogenic LAL assays for bacterial endotoxin quantification at an accredited ISO 17025 laboratory.
How should lyophilized VIP be stored upon receipt in the laboratory?
Lyophilized VIP should be stored desiccated at -20°C to -80°C away from light upon receipt. Stored under these conditions, the dry peptide maintains chemical stability for extended periods.
What diluent is recommended for reconstituting VIP for in vitro assays?
VIP can be reconstituted using sterile bacteriostatic water or sterile PBS (pH 7.4). For ultra-low working concentrations, adding 0.1% carrier protein (such as BSA) prevents peptide adsorption to container walls.
What is the molecular weight and sequence length of VIP?
Vasoactive Intestinal Peptide (VIP) is a 28-amino acid neuropeptide with a molecular weight of approximately 3325.8 Da and a C-terminal amide structure.
Are batch-specific Certificates of Analysis (COA) available?
Yes. Every shipment includes or provides direct access to a lot-specific COA containing full HPLC chromatograms, mass spectrometry spectra, and endotoxin assay results.
Does PX1 Research offer institutional or bulk purchasing options for VIP?
Yes, PX1 Research supports academic institutions and commercial laboratories with institutional pricing and bulk quantity fulfillment via our wholesale program.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.