5-Amino-1MQ Made in USA — Third-Party Verified
As a highly selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), 5-Amino-1MQ has emerged as a cornerstone compound in cellular metabolism and bioenergetics research. PX1 Research supplies high-purity 5-Amino-1MQ made in the USA, manufactured under strict GMP-compliant conditions and subjected to rigorous ISO 17025 independent laboratory testing. Designed exclusively for in vitro and preclinical research applications, our lot-verified material provides researchers with unmatched stability, chemical identity, and quantitative purity.
Quick answer
As a highly selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), 5-Amino-1MQ has emerged as a cornerstone compound in cellular metabolism and bioenergetics research. PX1 Research supplies high-purity 5-Amino-1MQ made in the USA, manufactured under strict GMP-compliant conditions and subjected to rigorous ISO 17025 independent laboratory testing. Designed exclusively for in vitro and preclinical research applications, our lot-verified material provides researchers with unmatched stability, chemical identity, and quantitative purity.
Key takeaways
- In modern bioenergetics research, nicotinamide N-methyltransferase (NNMT) represents a pivotal cytosolic enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), producing 1-methylnicotinamide (1-MNA).
- Nicotinamide adenine dinucleotide ([NAD+](/research-peptides/nad-plus)) is an essential coenzyme required for cellular oxidation-reduction reactions, sirtuin activation, and poly(ADP-ribose) polymerase (PARP) repair mechanisms.
- Mitochondrial dysfunction is a fundamental hallmark of metabolic decline and cellular senescence.
- Adipose tissue expressed high levels of NNMT in rodent models exhibiting diet-induced metabolic impairment.
Introduction to 5-Amino-1MQ and NNMT Inhibition
In modern bioenergetics research, nicotinamide N-methyltransferase (NNMT) represents a pivotal cytosolic enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), producing 1-methylnicotinamide (1-MNA). Elevated NNMT activity has been identified in preclinical models of metabolic dysfunction, obesity, and compromised mitochondrial dynamics, making NNMT targeted inhibition a critical focus for molecular biologists.
5-Amino-1-methylquinolinium (5-Amino-1MQ) is a membrane-permeable, small-molecule derivative engineered to potently and selectively inhibit NNMT. By blocking this enzymatic cascade, 5-Amino-1MQ prevents the irreversible degradation of nicotinamide, preserving the vital substrate necessary for the NAD+ salvage pathway. Laboratory protocols utilizing 5-Amino-1MQ seek to investigate the downstream cellular effects of NNMT inhibition without interfering with adjacent methyltransferase pathways or off-target kinase receptors.
Molecular Mechanism: Preserving Cellular NAD+ via Nicotinamide Salvage
Nicotinamide adenine dinucleotide (NAD+) is an essential coenzyme required for cellular oxidation-reduction reactions, sirtuin activation, and poly(ADP-ribose) polymerase (PARP) repair mechanisms. In pathological or hyper-metabolic states observed in rodent models, NNMT over-expression depletes the cellular pool of NAM by methylating it into 1-MNA, which is subsequently excreted rather than recycled.
In vitro data indicate that application of 5-Amino-1MQ effectively blocks NNMT catalytic function. By halting the methylation of NAM, cellular machinery retains higher availability of nicotinamide, directly stimulating the salvage pathway mediated by nicotinamide phosphoribosyltransferase (NAMPT). Preclinical studies suggest that this enzymatic blockade leads to a significant quantitative rise in intracellular NAD+ levels, establishing 5-Amino-1MQ as a premier tool for researchers investigating NAD+ salvage pathways and cellular longevity mechanisms.
Mitochondrial Bioenergetics and Mitochondrial Output Research
Mitochondrial dysfunction is a fundamental hallmark of metabolic decline and cellular senescence. The organelle's capacity to generate adenosine triphosphate (ATP) via oxidative phosphorylation is directly dependent on adequate mitochondrial NAD+/NADH ratios. When NNMT activity is blunted by 5-Amino-1MQ, the elevated intracellular NAD+ pool enhances the activity of mitochondrial sirtuins, particularly SIRT1 and SIRT3.
Preclinical investigations demonstrate that SIRT1 activation upregulates peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), the primary transcriptional coactivator responsible for mitochondrial biogenesis. In vitro models exposed to NNMT inhibitors display increased oxygen consumption rates (OCR), enhanced respiratory chain complex activity, and elevated basal ATP synthesis. Consequently, 5-Amino-1MQ serves as an essential probe for investigating mitochondrial output in cell culture and tissue explant assays.
Role in Fat-Metabolism Research and Adipose Tissue Dynamics
Adipose tissue expressed high levels of NNMT in rodent models exhibiting diet-induced metabolic impairment. Elevated NNMT in white adipose tissue (WAT) correlates with reduced intracellular NAD+ concentration, lowered basal metabolic rate, and diminished capacity for fatty acid oxidation. In preclinical animal studies, targeted inhibition of NNMT via systemic administration of 5-Amino-1MQ resulted in significant alterations in adipose tissue physiology.
Specifically, research models demonstrated enhanced basal lipolysis, increased expression of thermogenic uncoupling protein 1 (UCP-1), and a structural remodeling of white adipocytes toward a beige phenotype. Because 5-Amino-1MQ does not interact directly with central nervous system adrenergic receptors, its metabolic effects are mediated localized within peripheral tissues. This makes it a key compound in basic science protocols exploring fat-metabolism research, adipocyte differentiation, and lipid droplet clearance.
Comparative Analysis: 5-Amino-1MQ vs. Related Metabolic Research Compounds
When evaluating small molecules and peptides for metabolic and bioenergetic research protocols, investigators often compare 5-Amino-1MQ with other established regulators of energy homeostasis. While 5-Amino-1MQ acts specifically via NNMT inhibition to bolster NAD+ levels, mitochondrial peptide MOTS-c operates as a nuclear-translocating signal that regulates folate-dependent methionine metabolism and AMPK activation. Similarly, the synthetic estrogen-related receptor alpha agonist SLU-PP-332 targets transcriptional programs involved in oxidative muscle fiber transformation.
Another widely referenced metabolic compound, AICAR, directly phosphorylates AMPK, simulating cellular energy deprivation without altering the NAM/NNMT salvage pathway. In comparative in vitro assays, combining 5-Amino-1MQ with mitochondrial-targeted molecules such as SS-31 provides researchers with a novel dual-approach to analyze both inner mitochondrial membrane stabilization and NAD+-dependent enzyme activation simultaneously. Reviewing these pathways in the PX1 research library helps laboratories select the precise molecular tool for their targeted assay.
Why Domestic USA Synthesis Matters for Small Molecule Research
The supply chain for specialized laboratory research compounds is frequently compromised by overseas manufacturers utilizing inconsistent synthetic routes, unverified starter reagents, or inadequate purification protocols. Substandard chemical synthesis often yields heavy metal contamination, unreacted precursor contaminants, and salt isomer variations that disrupt sensitive cell culture assays and alter preclinical pharmacokinetic modeling.
PX1 Research addresses these vulnerabilities by offering 5-Amino-1MQ made in the USA. Domestic synthesis guarantees strict regulatory oversight, complete batch traceability, and adherence to American chemical manufacturing standards. Synthesized in USA-based GMP-compliant facilities, our 5-Amino-1MQ undergoes rigorous crystallization and wash cycles to ensure that investigators receive an ultrapure, standardized compound that produces reproducible results across longitudinal research studies.
ISO 17025 Verification & Lot-Specific HPLC/MS COA Integrity
Analytical integrity is the foundational requirement of valid scientific inquiry. PX1 Research mandates that every single lot of 5-Amino-1MQ synthesized in our domestic facilities undergoes independent third-party verification by an accredited ISO 17025 laboratory. We do not rely on manufacturer self-certification or outdated analytical templates.
Each batch is subjected to high-performance liquid chromatography (HPLC) to confirm chemical purity exceeds 98%, alongside Mass Spectrometry (MS) to verify precise molecular weight and structural identity. Furthermore, every lot is tested for bacterial endotoxins (LAL assay) to ensure suitability for delicate cellular models. Researchers can access a lot-specific Certificate of Analysis (COA) directly for every order, guaranteeing absolute transparency and scientific validity.
Laboratory Reconstitution and Handling Protocols
5-Amino-1MQ is provided as a highly purified, lyophilized solid or crystalline powder intended strictly for laboratory research use. To maintain the structural stability of the compound, reagents should be stored upon arrival at -20°C in a dry, light-protected environment. Exposure to repeated freeze-thaw cycles or ambient moisture should be avoided.
For in vitro research, 5-Amino-1MQ exhibits excellent solubility in dimethyl sulfoxide (DMSO) and moderate solubility in sterile aqueous buffers such as phosphate-buffered saline (PBS) depending on desired concentration. Laboratory personnel should utilize sterile, filter-sterilized solvent systems under a laminar flow hood when preparing stock solutions. Reconstituted stock solutions should be aliquoted into single-use polypropylene microcentrifuge tubes and stored at -80°C to preserve chemical activity over extended experimental timelines.
Institutional Sourcing and High-Volume Laboratory Accounts
Academic institutions, biotechnology enterprises, and contract research organizations (CROs) require dependable supply chains capable of delivering high-purity research compounds without batch-to-batch variability or shipping delays. PX1 Research operates state-of-the-art fulfillment facilities in California and Arizona, providing same-day shipping for orders processed Monday through Friday.
For large-scale preclinical trials or ongoing high-throughput screening projects, PX1 offers custom synthesis and bulk fulfillment options through our wholesale research portal. All institutional accounts receive direct access to raw HPLC chromatograms, mass spectra data files, and dedicated technical support to ensure seamless integration into university and enterprise laboratory workflows.
Frequently Asked Questions
What is the targeted mechanism of 5-Amino-1MQ in preclinical research?
5-Amino-1MQ functions as a highly selective inhibitor of nicotinamide N-methyltransferase (NNMT). In preclinical models, inhibiting NNMT prevents the irreversible degradation of nicotinamide (NAM), thereby raising cellular NAD+ levels and supporting enhanced mitochondrial energy production.
Where is PX1 Research's 5-Amino-1MQ manufactured?
PX1 Research provides 5-Amino-1MQ synthesized in the USA in GMP-compliant chemical manufacturing facilities, ensuring maximum chemical stability, batch consistency, and freedom from international supply chain impurities.
How is the purity of 5-Amino-1MQ verified?
Every lot of 5-Amino-1MQ undergoes independent third-party testing at an ISO 17025 accredited laboratory. Purity and identity are confirmed using High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), accompanied by bacterial endotoxin testing.
What analytical documents are included with a laboratory shipment?
Each shipment of 5-Amino-1MQ includes a lot-specific Certificate of Analysis (COA) detailing the exact HPLC purity percentage, mass spectrometry confirmation, lot number, and endotoxin assay results.
What solvents are recommended for reconstituting 5-Amino-1MQ for in vitro assays?
5-Amino-1MQ readily dissolves in organic solvents such as DMSO for high-concentration stock preparation. It can subsequently be diluted into sterile culture media or aqueous buffers like PBS, depending on specific experimental concentrations.
How does 5-Amino-1MQ compare to MOTS-c or SLU-PP-332 in metabolism studies?
5-Amino-1MQ specifically targets the cytosolic enzyme NNMT to preserve NAD+ salvage, whereas MOTS-c is a mitochondrial-derived peptide regulating nuclear gene expression and SLU-PP-332 acts as a nuclear receptor agonist for ERRα.
Are high-volume options available for institutional research accounts?
Yes, PX1 Research provides dedicated wholesale and bulk options for academic laboratories, CROs, and institutional accounts requiring larger quantities with matching lot integrity.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.