What is 5-Amino-1MQ?
5-Amino-1-methylquinolinium (5-Amino-1MQ) is a cell-permeable small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), not a peptide. It is supplied as a lyophilized powder for in-vitro metabolic research.
NNMT is highly expressed in adipose tissue and liver, and its inhibition is a well-documented approach for probing the NAD+ salvage pathway in cell culture.
Mechanism of action
NNMT consumes S-adenosylmethionine to methylate nicotinamide, diverting nicotinamide away from NAD+ resynthesis. Inhibiting NNMT with 5-Amino-1MQ raises intracellular nicotinamide and, in adipocyte models, has been reported to increase NAD+ and sirtuin-1 activity.
Because the readouts overlap with NAD+ precursor work, 5-Amino-1MQ is frequently studied in parallel with NAD+ itself to distinguish salvage-pathway effects from direct cofactor supplementation.
Laboratory handling and storage
5-Amino-1MQ ships as a lyophilized powder in a sealed, tamper-evident vial. Store the unopened vial between −20°C and −80°C, protected from light, until the day of use. In this state the peptide is stable through the labeled expiration date printed on the lot documentation.
Allow the vial to reach room temperature before opening so atmospheric moisture does not condense onto the lyophilized cake. Introduce bacteriostatic or sterile water slowly against the vial wall, then swirl — never shake — until the solution is clear. Store the reconstituted solution at 2–8°C, aliquot to avoid repeated freeze–thaw cycles, and record the lot number in your laboratory notebook alongside the reconstitution date.
Purity, testing and Certificate of Analysis
Every PX1 Research lot of 5-Amino-1MQ is manufactured in the United States and released against a documented specification of ≥99% purity by reversed-phase HPLC, with identity confirmed on the same lot by high-resolution LC-MS against the theoretical mass of the sequence.
Release testing covers six layers: HPLC purity, LC-MS identity, endotoxin by kinetic chromogenic LAL, residual solvents by gas chromatography, water content by Karl Fischer titration, and visual inspection of appearance and reconstitution. The batch-specific Certificate of Analysis is published on this page, and the lot number on the vial label matches the report so the material in your hand traces back to the data you can read online.
Research use only
5-Amino-1MQ supplied by PX1 Research is intended strictly for in-vitro laboratory and research applications. It is not a drug, food, cosmetic, or medical device, and it is not for human or veterinary use, diagnosis, treatment, or consumption.
Researchers are responsible for handling the compound in accordance with their institution's safety procedures and all applicable local regulations. Safety data sheets and current lot documentation are available on this site.
References
- Neelakantan 2018. Neelakantan H, et al. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high fat diet-induced obesity in mice. Biochemical Pharmacology. 2018;147:141-152.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.