High-Volume Ancillaries
Every lyophilized peptide needs a diluent. This page covers what sterile water and bacteriostatic water actually do, which one belongs in a multi-draw protocol, and how diluent volume determines the concentration you draw. For laboratory research use only.
| Property | Sterile Water | Bacteriostatic Water |
|---|---|---|
| Preservative | None | 0.9% benzyl alcohol |
| Draws per vial | Single session | Repeated draws |
| Shelf life once punctured | Use immediately | ~28 days at 2–8°C |
| Typical use | Assay-sensitive single dilutions | Standard multi-draw research protocols |
Convert vial strength and water volume into exact insulin-syringe unit marks.
Peptide reconstitution calculator
Concentration
5 mg/mL
Draw volume
0.05 mL
Insulin syringe
5 units (IU)
U-100 insulin syringe (100 units = 1 mL)
10 mg + 2 mL = 5 mg/mL. A 250 mcg dose = 5 units on a U-100 syringe (40 doses per vial).
For laboratory and research use only. Not for human or veterinary use.
Sterile water contains no preservative, so a reconstituted vial should be used immediately in a single session. Bacteriostatic water contains 0.9% benzyl alcohol, which suppresses microbial growth and allows a reconstituted vial to be drawn from repeatedly over roughly 28 days under refrigeration.
Bacteriostatic water. Any protocol where the same vial is accessed more than once benefits from the preservative; sterile water is reserved for single-session work or where benzyl alcohol would interfere with an assay.
Diluent volume sets concentration, not dose. A 10mg vial with 2mL of water yields 5mg/mL, so 10 units on an insulin syringe equals 0.5mg. Use the calculator below to convert any strength and volume into unit marks.
Store unopened vials at room temperature away from light. After the stopper is punctured, keep bacteriostatic water refrigerated at 2–8°C and discard after 28 days.
10mL suits a single-compound protocol. 30mL is more economical when several lyophilized vials are being reconstituted across a research program.
