What is Retatrutide (PX1-R)?

Retatrutide is a synthetic 39-amino-acid peptide engineered as a triple receptor agonist, acting at the GLP-1, GIP and glucagon receptors. A fatty-acid side chain promotes albumin binding, which extends its circulating half-life relative to unmodified incretin peptides. PX1 Research supplies it under the PX1-R designation as a lyophilized research powder.

How does Retatrutide differ from Tirzepatide?

Tirzepatide (PX1-T) is a dual agonist at the GLP-1 and GIP receptors. Retatrutide adds a third arm — glucagon receptor agonism — which is the defining structural and pharmacological difference studied in the literature between the two compounds.

How should Retatrutide be reconstituted?

Add bacteriostatic water slowly down the inner vial wall rather than directly onto the lyophilized cake, then swirl gently until fully dissolved — never shake. A 10mg vial with 2.0 mL of diluent yields 5 mg/mL. The reconstitution table above covers the common vial strengths.

How long is reconstituted Retatrutide stable?

Reconstituted with bacteriostatic water and refrigerated at 2–8 °C, laboratory stability is typically referenced at up to roughly 28–30 days. Sterile water contains no preservative and is generally referenced at 24–48 hours. Protect from light and avoid freeze/thaw cycling.

How is purity verified?

Every PX1 Research lot ships against a batch-specific certificate of analysis with HPLC purity, LC-MS mass confirmation, and third-party ISO/IEC 17025 accredited lab testing. COAs are published publicly and searchable by batch number rather than supplied only on request.

Is PX1-R manufactured in the United States?

Yes. It is synthesized, purified and lyophilized in our US cGMP-compliant facility and dispatched from a US warehouse. It is supplied strictly for in-vitro laboratory research use and is not for human or veterinary use.

Compound reference

Retatrutide (PX1-R): research overview, reconstitution & sourcing

Retatrutide is a synthetic 39-amino-acid peptide studied as a triple agonist at the GLP-1, GIP and glucagon receptors. This page consolidates the molecular profile, reconstitution mathematics, storage handling and purity documentation behind every PX1-R lot.

US cGMP facility

≥99% HPLC purity

Public batch COAs

Same-day dispatch

View PX1-R vials See batch COAs

Molecular & product profile

PX1 designation	PX1-R
Compound class	Triple agonist peptide — GLP-1 / GIP / glucagon receptors
Structure	39-amino-acid synthetic peptide with a fatty-acid (C20 diacid) side chain
Molecular formula	C221H342N46O68
Molar mass	≈ 4,731 g/mol
CAS number	2381089-83-2
Presentation	Lyophilized powder — 10mg, 20mg, 30mg and 60mg vials
Purity specification	≥ 99% by HPLC, confirmed by LC-MS
Storage (lyophilized)	-20 °C long term; 2–8 °C short term

Reconstitution reference

Concentration is peptide mass divided by diluent volume. Add bacteriostatic water slowly against the vial wall, swirl until the cake fully dissolves, and confirm the solution is clear and particulate-free before use.

2.0 mL bacteriostatic water

5 mg/mL (5,000 mcg/mL)

10mg PX1-R vial

10 units (0.1 mL) = 500 mcg

2.0 mL bacteriostatic water

10 mg/mL (10,000 mcg/mL)

20mg PX1-R vial

10 units (0.1 mL) = 1,000 mcg

3.0 mL bacteriostatic water

10 mg/mL (10,000 mcg/mL)

30mg PX1-R vial

10 units (0.1 mL) = 1,000 mcg

Use the reconstitution calculator →

Storage & handling

Lyophilized: store at -20 °C for long-term holding; 2–8 °C is acceptable for short-term working inventory. Keep sealed and protected from light.
Reconstituted: refrigerate at 2–8 °C. Bacteriostatic water (0.9% benzyl alcohol) is referenced at up to ~28–30 days; sterile water at 24–48 hours.
Avoid: vigorous shaking, repeated freeze/thaw cycling, and direct diluent impact on the lyophilized cake — all accelerate peptide degradation.
Verification: a clear, colorless solution is expected. Cloudiness or visible particulate indicates contamination or degradation.

Retatrutide vs Tirzepatide at a glance

Attribute	Retatrutide (PX1-R)	Tirzepatide (PX1-T)
Receptor targets	GLP-1 / GIP / glucagon	GLP-1 / GIP
Agonist type	Triple	Dual
Chain length	39 amino acids	39 amino acids
Half-life extension	C20 diacid fatty-acid side chain	C20 fatty diacid side chain

Sourcing & documentation

PX1 Research is a primary manufacturer, not a reseller. PX1-R is synthesized, purified and lyophilized in our US cGMP-compliant facility, then tested by an ISO/IEC 17025 accredited third-party laboratory. Each lot ships with a batch-matched certificate of analysis covering HPLC purity, LC-MS identity, endotoxin, and sterility screening — published publicly rather than supplied on request.

Frequently asked questions

Retatrutide (PX1-R) vial

10mg / 20mg / 30mg / 60mg lyophilized

Retatrutide sublingual strips

Needle-free dissolving strip format

Retatrutide category hub

All formats, strengths and pricing

Reconstitution guide

Step-by-step dilution walkthrough

Reconstitution calculator

Solve any vial size and target dose

Certificates of analysis

Search published COAs by batch

Research use only. Products described on this page are supplied strictly for in-vitro laboratory research and are not for human or veterinary use, not for diagnostic procedures, and have not been evaluated by the U.S. Food and Drug Administration. Nothing here is medical advice.