FOXO4-DRI For Sale — Research Grade
High-purity FOXO4-DRI is now available for specialized cellular aging, p53 signaling, and senolytic research applications. Synthesized in the USA within GMP-compliant facilities, PX1 Research supplies laboratory-grade compounds featuring lot-specific analytical verification via HPLC and mass spectrometry. Every order ships same-day from our California and Arizona facilities to ensure uninterrupted experimental timelines.
Quick answer
High-purity FOXO4-DRI is now available for specialized cellular aging, p53 signaling, and senolytic research applications. Synthesized in the USA within GMP-compliant facilities, PX1 Research supplies laboratory-grade compounds featuring lot-specific analytical verification via HPLC and mass spectrometry. Every order ships same-day from our California and Arizona facilities to ensure uninterrupted experimental timelines.
Key takeaways
- FOXO4-DRI (Forkhead box O4 D-retro-inverso) is a synthetic D-amino acid peptide designed to selectively perturb the interaction between the FOXO4 transcription factor and the p53 tumor suppressor protein.
- The primary challenge with standard L-amino acid peptides in cell culture and preclinical models is rapid enzymatic degradation by endogenous proteases.
- Preclinical studies indicate that the binding affinity between wild-type FOXO4 and p53 is crucial for maintaining senescent cell viability.
- When purchasing [FOXO4-DRI for sale](/product/foxo4-dri) for quantitative assays, peptide purity directly dictates reproducibility.
Overview of FOXO4-DRI in Cellular Senescence Research
FOXO4-DRI (Forkhead box O4 D-retro-inverso) is a synthetic D-amino acid peptide designed to selectively perturb the interaction between the FOXO4 transcription factor and the p53 tumor suppressor protein. In healthy cellular environments, p53 regulates DNA repair, cell cycle arrest, and apoptosis. However, in senescent cells—cells that have ceased dividing but remain metabolically active—p53 is sequestered by FOXO4, preventing programmed cell death and driving the secretion of pro-inflammatory cytokines known as the senescence-associated secretory phenotype (SASP).
By competing for the binding domain on p53, the FOXO4-DRI peptide liberates p53 to translocate to the mitochondria, triggering nuclear exclusion and initiating intrinsic apoptotic pathways specifically in senescent cell populations. Because senescent cell accumulation is implicated in organ dysfunction, tissue fibrosis, and microenvironment degradation, FOXO4-DRI serves as a critical molecular tool in modern senolytic research compounds and longevity models.
Structural Engineering: The D-Retro-Inverso Modification
The primary challenge with standard L-amino acid peptides in cell culture and preclinical models is rapid enzymatic degradation by endogenous proteases. FOXO4-DRI addresses this pharmacokinetic limitation through a D-retro-inverso modification. This chemical engineering strategy involves two distinct structural inversions: reversing the peptide sequence order (retro) and substituting all natural L-amino acids with their enantiomeric D-amino acid counterparts (inverso).
This modification preserves the side-chain spatial topology necessary for high-affinity binding to the p53 interface while rendering the peptide backbone virtually invisible to proteolytically active peptidases. As a result, in vitro and animal assays demonstrate markedly extended half-life profiles in physiological buffers and cell culture media, allowing investigators to observe target interactions without confounded degradation kinetics.
Targeted Disruption of the FOXO4-p53 Axis
Preclinical studies indicate that the binding affinity between wild-type FOXO4 and p53 is crucial for maintaining senescent cell viability. In non-senescent, proliferating cells, FOXO4 levels are strictly regulated, and p53 operates within conventional homeostatic loops. In senescent phenotypes, however, elevated FOXO4 sequesters active p53 away from apoptotic induction centers.
In vitro data demonstrate that application of FOXO4-DRI disrupts this complex, releasing p53 to interact with pro-apoptotic proteins such as Bax and Bak at the outer mitochondrial membrane. Crucially, preclinical models suggest that non-senescent cells remain unaffected by FOXO4-DRI exposure, as healthy cells do not rely on FOXO4 hyper-interaction for survival. This selective mechanism makes FOXO4-DRI a primary focus within our broader research library dedicated to targeted cellular fate pathways.
Sourcing High-Purity FOXO4-DRI: Chemical Quality and Specs
When purchasing FOXO4-DRI for sale for quantitative assays, peptide purity directly dictates reproducibility. Impurities such as truncated peptide fragments, incomplete sequence iterations, or residual synthesis solvents can alter cell viability baselines, generate off-target toxicity, or yield non-reproducible binding kinetics.
PX1 Research manufactures all custom research peptides within USA-based, ISO 17025-accredited laboratories operating under strict quality assurance frameworks. Each lot of FOXO4-DRI undergoes multi-stage purification via preparative High-Performance Liquid Chromatography (HPLC) to guarantee a purity threshold exceeding 98.0%. Synthetic identity is simultaneously validated through electrospray ionization mass spectrometry (ESI-MS) to ensure exact molecular weight match and full sequence integrity.
Comparative Analysis: FOXO4-DRI vs. Parallel Metabolic & Senolytic Peptides
Investigators exploring cellular preservation, mitochondrial function, and tissue homeostasis frequently evaluate FOXO4-DRI alongside other targeted research compounds. While FOXO4-DRI acts specifically on the p53-mediated apoptotic cascade in senescent cells, mitochondrial-targeted peptides address cellular aging through complementary bioenergetic pathways.
For example, the mitochondrial-derived peptide MOTS-c modulates metabolic homeostasis and insulin sensitivity via AMPK activation, whereas SS-31 (Elamipretide) selectively targets cardiolipin in the inner mitochondrial membrane to reduce reactive oxygen species (ROS) production. Similarly, Epitalon is investigated for its potential interaction with telomerase activity and chromatin structure. Integrating FOXO4-DRI into multi-compound cellular longevity models allows research teams to isolate senolytic clearance mechanisms from pure bioenergetic or protective signaling cascades.
Analytical Verification: HPLC and Mass Spectrometry Documentation
At PX1 Research, transparency and analytical rigor are absolute parameters. We do not rely on batch-averaged reports or manufacturer self-certifications. Every individual production lot of FOXO4-DRI is paired with a lot-specific Certificate of Analysis (COA) accessible directly by laboratory personnel.
The COA documents chromatograms displaying sharp peak resolution, verifying high purity without hidden shoulder peaks representing sequence deletion variants. Mass spectrometry reports further verify the theoretical molecular mass against experimental values, ensuring that D-retro-inverso modification parameters are fully satisfied. Research teams can review analytical data prior to experimental deployment to ensure absolute standardization across longitudinal studies.
Endotoxin Control and Sterility Standards
In cell culture assays and preclinical models, bacterial endotoxin contamination (lipopolysaccharides) presents a major confounding variable. Endotoxins trigger toll-like receptor 4 (TLR4) activation, inducing unwanted pro-inflammatory cytokine cascades that mimic or obscure senescence-associated secretory phenotype (SASP) measurements.
To mitigate this risk, PX1 Research implements rigorous depyrogenation workflows and validates endotoxin limits using standardized Limulus Amebocyte Lysate (LAL) testing protocols. Every lot of FOXO4-DRI is guaranteed to fall below strict EU/mg endotoxin thresholds, rendering the material fully compatible with sensitive primary cell lines, organoid cultures, and animal models.
Reconstitution, Handling, and Storage Protocols for Laboratory Use
FOXO4-DRI is supplied as a lyophilized (freeze-dried) powder to maximize long-term chemical stability. To maintain structural integrity upon arrival, lyophilized vials should be stored at -20°C or -80°C in a dry environment protected from light exposure.
For reconstitution in laboratory settings, researchers should allow the vial to equilibrate to room temperature inside a laminar flow hood before introducing solvent to avoid condensation. Reconstitution should be performed using sterile, bacteriostatic water or laboratory-grade phosphate-buffered saline (PBS), depending on experimental protocols. Gentle swirly agitation—avoiding vigorous vortexing or sonication—is recommended to fully dissolve the peptide. Once reconstituted, aliquoting into single-use experimental volumes minimizes freeze-thaw degradation cycles.
Logistics and Shipping Reliability: Same-Day Dispatch
Experimental workflows rely on consistent material delivery. PX1 Research maintains complete stock availability for research institutions and university laboratories across North America. Orders placed Monday through Friday before cut-off times ship same-day directly from our centralized distribution hubs in California and Arizona.
Peptide shipments are packaged in climate-controlled temperature-insulated containers to ensure protection against environmental temperature spikes during transit. Fast domestic fulfillment reduces transport stress on the product, ensuring that lyophilized cake geometry and compound stability remain uncompromised upon arrival.
Institutional Procurement and B2B Research Accounts
PX1 Research supports high-throughput academic laboratories, biotechnology enterprises, and contract research organizations (CROs) requiring reliable recurring supply. Through our dedicated wholesale program, institutional buyers gain access to scaled volume pricing, dedicated account management, and reserved lot allocation to ensure lot-to-lot continuity across multi-year research grants.
Whether purchasing single analytical units or establishing custom supply contracts, PX1 Research offers flexible procurement options backed by full compliance documentation and scientific support.
Frequently Asked Questions
What is the purity level of FOXO4-DRI available from PX1 Research?
PX1 Research supplies FOXO4-DRI with a verified purity threshold of ≥98.0%, confirmed via lot-specific High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS).
Is FOXO4-DRI approved for human use or clinical administration?
No. FOXO4-DRI is strictly a research compound sold exclusively for in vitro, laboratory, and preclinical research applications. It is not intended for human consumption, therapeutic use, or diagnostic administration.
How does PX1 Research test for endotoxins in FOXO4-DRI lots?
Each lot undergoes Limulus Amebocyte Lysate (LAL) endotoxin testing to verify that endotoxin levels remain below strict laboratory safety thresholds (typically <0.1 EU/mg), ensuring compatibility with cell culture and animal models.
From where does PX1 Research ship FOXO4-DRI orders?
All orders are fulfilled directly from our facility locations in California and Arizona. Orders placed Monday through Friday ship same-day.
How should lyophilized FOXO4-DRI be stored upon receipt?
Lyophilized FOXO4-DRI should be stored at -20°C or -80°C in a desiccated, dark environment. Reconstituted solutions should be aliquoted and stored at -80°C to prevent degradation from repeated freeze-thaw cycles.
What solvent should be used to reconstitute FOXO4-DRI for in vitro assays?
Depending on experimental design, sterile laboratory-grade bacteriostatic water, sterile saline, or buffered solutions like PBS are typically utilized. Researchers should select solvents compliant with their specific cell culture protocols.
What makes the D-retro-inverso modification important in FOXO4-DRI research?
The D-retro-inverso modification reverses the peptide sequence and utilizes D-amino acids, making the molecule highly resistant to proteolytic cleavage by peptidases while maintaining target binding specificity to p53.
Can universities and corporate laboratories set up bulk or wholesale supply accounts?
Yes. Institutional buyers and CROs can establish wholesale accounts with PX1 Research to access volume pricing, dedicated lot reservations, and institutional billing terms.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.