Access complete, lot-specific analytical documentation for PT-141 (Bremelanotide) research compounds. Every certificate of analysis provides verified high-performance liquid chromatography (HPLC) chromatograms, mass spectrometry (MS) characterization, and quantitative endotoxin testing to ensure consistent experimental reproducibility across preclinical research settings.
Access complete, lot-specific analytical documentation for PT-141 (Bremelanotide) research compounds. Every certificate of analysis provides verified high-performance liquid chromatography (HPLC) chromatograms, mass spectrometry (MS) characterization, and quantitative endotoxin testing to ensure consistent experimental reproducibility across preclinical research settings.
A valid pt-141-coa provides empirical documentation of compound identity, absolute purity, and residual chemical safety for laboratory research use only. Every batch of synthetic PT-141 produced for PX1 Research undergoes rigorous third-party analytical evaluation at an ISO 17025 accredited laboratory, guaranteeing that physical and chemical metrics align precisely with research standards prior to distribution.
Researchers seeking to verify a specific lot can cross-reference the batch number printed on the vial label with our centralized quality control archive. Reviewing the complete PT-141 research compound documentation ensures that experimental protocols involving melanocortin receptor activation are conducted with fully characterized reagents free from batch-to-batch variation.
A comprehensive Certificate of Analysis (COA) for PT-141 (Bremelanotide, cyclic peptide structure Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-OH) outlines key physicochemical characteristics essential for valid experimental design. Primary analytical fields include target sequence identity, molecular formula, calculated vs. observed molecular weight, and individual impurity profiling.
The theoretical monoisotopic mass of PT-141 is 1024.18 Da. On a standard PT-141 COA, electrospray ionization mass spectrometry (ESI-MS) or matrix-assisted laser desorption/ionization (MALDI-TOF) testing confirms the exact molecular mass, demonstrating the absence of structural truncations or unintended amino acid substitutions during solid-phase peptide synthesis (SPPS).
Purity determination is performed using reverse-phase high-performance liquid chromatography (RP-HPLC). The main peak integration must meet or exceed a 99.0% threshold, with specific quantification of secondary peaks representing residual TFA, deletion sequences, or oxidation products. For detailed methodology regarding chromatographic peak resolution, researchers can review our guide on peptide purity HPLC analysis.
Quantitative verification of research-grade peptides relies on robust separation techniques. High-performance liquid chromatography utilizes a C18 stationary phase combined with an aqueous acetonitrile gradient containing 0.1% trifluoroacetic acid (TFA) as an ion-pairing reagent. Detection at 214 nm or 220 nm allows sensitive measurement of the peptide backbone amide bonds, capturing even trace impurities.
Mass spectrometry analysis runs concurrently to confirm compound identity beyond chromatographic retention time. By ionizing the purified sample, the mass spectrometer measures the mass-to-charge ratio (m/z), confirming the presence of the singly charged [M+H]+ or doubly charged [M+2H]2+ ions corresponding to Bremelanotide.
All analytical data captured during testing are archived in our broader peptide research database, establishing full transparency and traceability from raw material synthesis to finished lyophilized vial packaging.
Bacterial endotoxins—primarily lipopolysaccharides (LPS) derived from Gram-negative outer cell membranes—can interfere significantly with cell culture viability, receptor binding assays, and signal transduction studies. A reliable pt-141-coa must document quantitative endotoxin levels to ensure non-interference in sensitive bioassays.
PX1 Research enforces strict limits using the Limulus Amebocyte Lysate (LAL) chromogenic assay. Every batch must display an endotoxin content under 0.01 EU/mg, far surpassing general industry baselines. Experimentalists evaluating cellular response pathways can consult our dedicated resource on endotoxin testing standards to understand how low bioburden protects assay validity.
PT-141 is a synthetic cyclic peptide analogue of alpha-melanocyte-stimulating hormone (α-MSH) that functions as a non-selective agonist at melanocortin receptors, primarily MC3R and MC4R, with secondary affinity for MC1R and MC5R. In preclinical literature, melanocortin signaling pathways are investigated for their involvement in central nervous system regulation of physiological signaling related to sexual health pathways and vascular tone.
When designing comparative in vitro or animal studies, researchers frequently compare PT-141 against related peptide structures in the same class. For example, Melanotan II displays potent activity across MC1R, MC3R, MC4R, and MC5R, but possesses a distinct hydrophobic profile that impacts receptor selectivity and peripheral responses. Conversely, non-selective native peptides like α-MSH exhibit shorter half-lives in biological matrices.
Evaluating compound profiles across our complete catalog of melanocortin agonists allows investigators to select the precise ligand needed for target receptor binding studies, signal transduction mapping, or metabolic signaling assays.
Not all analytical reports offer equal credibility. A valid COA must originate from an independent, ISO/IEC 17025 accredited laboratory operating under rigorous quality control management systems. This certification ensures that instruments are routinely calibrated, testing methods are validated, and data integrity is maintained without conflict of interest.
PX1 Research manufactures all compounds in cGMP-compliant facilities within the United States. Every lot is subjected to dual-tier verification: internal quality checks following solid-phase synthesis and subsequent independent third-party analysis. This multi-step pipeline guarantees that every vial shipped from our California and Arizona logistics facilities fulfills exact specification criteria.
To maintain the structural integrity demonstrated on the pt-141-coa, research personnel must observe strict handling guidelines upon receipt. Lyophilized peptide cakes should be stored at -20°C in a dry, dark environment to prevent thermal degradation or moisture absorption.
When preparing solutions for laboratory assays, reconstitution should be performed using sterile laboratory solvents such as bacteriostatic water or phosphate-buffered saline (PBS). Avoid vigorous agitation; gentle swirling minimizes mechanical shear stress on the cyclic peptide structure. For precise volumetric calculations and molar concentrations, utilize our peptide reconstitution calculator.
Once reconstituted, aliquots should be stored at 2°C to 8°C for short-term experimental series or frozen at -80°C to avoid repeated freeze-thaw cycles that can induce peptide aggregation or amide hydrolysis.
When auditing supplier documentation for research peptides, principal investigators should evaluate the following mandatory parameters on the accompanying analytical certificate:
1. HPLC Purity: Confirmed ≥99.0% area under the curve (AUC) at 214 nm. 2. Mass Spectrum Verification: Mass peak matching the theoretical molecular weight (1024.18 Da) within acceptable resolution tolerances. 3. Endotoxin Quantification: Measured LAL assay results below 0.01 EU/mg. 4. Physical Appearance: White to off-white lyophilized cake, free from particulate matter or discoloration. 5. Solubility & Clear Solution Test: Complete dissolution in aqueous buffers without persistent turbidity.
Reviewing these criteria ensures that experimental datasets remain robust, publication-ready, and reproducible across different laboratory environments. Browse our full list of research peptides to view standard quality specifications across all available catalog items.
For academic institutions, pharmaceutical research facilities, and contract research organizations (CROs) requiring large-scale peptide supply, lot uniformity is paramount. Variations between synthesis runs can introduce uncontrolled variables into long-term preclinical investigations.
PX1 Research supports high-volume research requirements by reserving single-lot batches with pre-issued quality certificates. Qualified institutions can establish dedicated supply lines through our wholesale laboratory account program, securing standardized compounds, custom analytical documentation, and expedited same-day dispatch from our US fulfillment hubs.
How do I perform a pt-141-coa lot lookup?
To locate the Certificate of Analysis for your batch, match the lot number printed on the vial label with the COA lookup tool on the PX1 Research website, or contact our support team to request a PDF of the third-party HPLC/MS report.
What analytical data should be included on a PT-141 Certificate of Analysis?
A valid PT-141 COA must include RP-HPLC purity chromatograms, mass spectrometry (MS) characterization confirming molecular weight (1024.18 Da), LAL endotoxin testing results (<0.01 EU/mg), physical appearance, and lot identification numbers.
What is the standard HPLC purity threshold for research-grade PT-141?
PX1 Research enforces a minimum purity threshold of ≥99.0% as determined by reverse-phase HPLC peak integration at 214 nm for all PT-141 research compounds.
How is the molecular weight of PT-141 verified via mass spectrometry?
Mass spectrometry (ESI-MS or MALDI-TOF) measures the mass-to-charge ratio of the peptide, confirming structural identity against the theoretical monoisotopic mass of 1024.18 Da.
Why is endotoxin testing critical for PT-141 in laboratory assays?
Endotoxins can trigger non-specific inflammatory signaling or cell death in vitro. Quantitative LAL testing ensures endotoxin levels remain below 0.01 EU/mg, preventing experimental artifact creation.
How should lyophilized PT-141 be stored upon delivery to the lab?
Lyophilized PT-141 vials should be stored at -20°C in a desiccated environment protected from light to maintain chemical stability prior to reconstitution.
What solvent is recommended for reconstituting PT-141 for in vitro protocols?
PT-141 is typically reconstituted using bacteriostatic water, sterile normal saline, or phosphate-buffered saline (PBS), depending on the requirements of the specific cell culture or assay protocol.
How does PT-141 differ from Melanotan II in receptor selectivity?
While both are melanocortin receptor agonists derived from α-MSH, PT-141 (Bremelanotide) exhibits higher functional selectivity for central MC3R and MC4R pathways relative to peripheral MC1R responses compared to Melanotan II.
Are PX1 Research PT-141 lots manufactured in the USA?
Yes. All PX1 Research peptides are manufactured in cGMP-compliant facilities within the United States and undergo independent verification at ISO 17025 accredited testing laboratories.
Can institutional buyers request batch-specific COAs prior to bulk orders?
Yes. Laboratory purchasing managers can contact PX1 Research directly or submit an inquiry through our wholesale portal to review lot-specific analytical reports prior to placing bulk orders.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.