CJC-1295 (No DAC) 50mg — Vial Spec, Reconstitution Math & COA

Overview of CJC-1295 (No DAC) 50mg Format & Catalog Availability

CJC-1295 (No DAC), also known as Modified GRF (1-29), is a synthetic 29-amino-acid peptide analog of naturally occurring Growth Hormone-Releasing Hormone (GHRH). The 50mg vial format represents a bulk analytical mass designed for large-scale laboratory workflows, longitudinal rodent studies, or continuous automated dosing systems where lower mass vials (such as 2mg or 5mg formats) require excessive physical reconstitutions and risk introduced inter-vial variance.

Note on Catalog Availability: PX1 Research primary stocking configurations feature standardized analytical sizes, such as our standard CJC-1295 No DAC product page 2mg and 5mg vials, which minimize peptide degradation risks associated with extended reconstituted storage. High-volume 50mg vials are made available upon institutional request or specialized high-throughput catalog runs across our full catalog of all peptides. Researchers requiring bulk batch allocations for high-capacity laboratory protocols can coordinate dedicated batch reserve schedules through our dedicated enterprise team.

Molecular Structure, Receptor Kinetics, and Mechanism of Action

CJC-1295 (No DAC) maintains a molecular formula of C152H252N44O42 and a molecular mass of approximately 3367.2 Da. It incorporates four strategic amino acid substitutions relative to wild-type GHRH(1-29): D-alanine at position 2, glutamine at position 8, alanine at position 15, and leucine at position 27. These modification sites significantly improve enzymatic resistance against dipeptidyl peptidase-IV (DPP-IV) cleavage without altering high-affinity binding to the GHRH receptor on anterior pituitary somatotrophs.

Unlike CJC-1295 with DAC (Drug Affinity Complex)—which binds covalently to circulating serum albumin to extend half-life to several days—CJC-1295 (No DAC) exhibits rapid, pulsatile receptor activation. In vitro and animal models demonstrate that CJC-1295 (No DAC) has a biological half-life of roughly 30 minutes in vivo. This allows researchers to mimic physiological endogenous GHRH pulses without causing continuous somatotroph receptor desensitization or altering down-stream feedback inhibition loops mediated by somatostatin.

Preclinical data indicate that signaling via the GHRH receptor activates the adenylate cyclase-cAMP-protein kinase A pathway, stimulating the transcription and secretion of endogenous Growth Hormone (GH). Downstream target activation includes hepatic synthesis of Insulin-like Growth Factor 1 (IGF-1), making this compound a primary tool for investigating tissue repair mechanisms, extracellular matrix deposition, nitrogen retention, and cellular regeneration in preclinical models.

Reconstitution Math & Concentration Protocols for 50mg Vials

Handling a high-mass 50mg lyophilized vial requires accurate volumetric calculation to ensure targeted microgram delivery per volumetric microliter without introducing standard deviation across operational samples. Because 50mg is a substantial mass, selecting the appropriate volume of diluent (typically Bacteriostatic Water or Sterile Normal Saline) directly dictates liquid viscosity and micro-pipetting accuracy.

Below are standard volumetric dilution models for a 50mg CJC-1295 (No DAC) lyophilized cake:

1. Reconstitution with 1.0 mL Diluent: Yields a concentration of 50.0 mg/mL (50.0 µg/µL). At this concentration, 10 µL of solution yields 500 µg of active compound. This concentrated formulation is ideal for automated micro-dispensing platforms or high-concentration in vitro well-plate assays.

2. Reconstitution with 2.0 mL Diluent: Yields a concentration of 25.0 mg/mL (25.0 µg/µL). At this concentration, 20 µL yields 500 µg (or 10 µL = 250 µg). This concentration balances solution viscosity with pipetting precision across standard multi-channel pipettes.

3. Reconstitution with 5.0 mL Diluent: Yields a concentration of 10.0 mg/mL (10.0 µg/µL). At this concentration, 50 µL yields 500 µg (or 10 µL = 100 µg). This lower concentration is frequently preferred for preclinical animal dosing protocols where larger working volumes minimize percentage-based volumetric error.

To quickly verify custom reconstitution volumes and target concentrations for specialized lab setups, researchers should utilize our online reconstitution calculator tool before reconstitution.

Aliquot Planning, Handling, and Long-Term Thermal Storage

Because reconstituted peptides degrade over time through hydrolytic cleavage and oxidation, reconstituting a 50mg vial all at once requires a strict aliquoting procedure unless consumed immediately in automated assays. Repeated freeze-thaw cycles break peptide backbones, generating fragments that alter research outcomes.

After slowly introducing diluent down the glass wall of the vial and allowing the lyophilized powder to dissolve via gentle swirl (never vigorous shaking), immediately aliquot the solution into single-use, polypropylene low-binding microcentrifuge tubes. Ensure aliquots reflect the exact microgram quantities needed for a single operational assay run.

Lyophilized CJC-1295 (No DAC) should be stored upon arrival at -20°C for short-term evaluation or -80°C for multi-year preservation, protected from light exposure. Reconstituted liquid aliquots must be kept at 2°C to 8°C if utilized within 14 to 28 days (when prepared with Bacteriostatic Water containing 0.9% benzyl alcohol). Frozen liquid aliquots kept at -80°C maintain high chemical stability for several months; however, aliquots must only be thawed once immediately prior to assay administration.

Comparative Analysis: CJC-1295 (No DAC) vs. Related GHRH & Secretague Peptides

When evaluating candidates within the growth hormone secretagogue domain, researchers often compare CJC-1295 (No DAC) against structural variants and ghrelin receptor agonists to identify the optimal physiological tool for their protocol.

In contrast to Sermorelin, which is the truncated 29-amino-acid native sequence of GHRH, CJC-1295 (No DAC) incorporates four substituted amino acids that enhance plasma half-life and enzymatic stability while preserving pulsatile receptor interaction. When evaluated alongside Ipamorelin, a selective growth hormone secretagogue receptor (GHSR-1a) agonist, CJC-1295 (No DAC) operates via a completely distinct receptor pathway (GHRHR vs. GHSR), making synergistic co-administration a common subject of preclinical study. Finally, compared to Tesamorelin, an N-terminally modified GHRH analog with specific lipolytic signaling profiles, CJC-1295 (No DAC) exhibits a broader pulsatile secretory dynamic in rodent metabolic models. For comprehensive data on secretagogue mechanics, explore our main research hub.

Quality Control Protocols: Third-Party HPLC, MS, and Endotoxin Testing

In preclinical research, trace impurities, synthesis reagents, or endotoxins can alter cellular signaling, induce non-specific inflammatory responses, or skew physiological trial data. PX1 Research subjects every production lot to rigorous quality verification performed by independent ISO 17025 accredited analytical laboratories.

Chemical purity is confirmed via High-Performance Liquid Chromatography (HPLC), guaranteeing that active peptide mass meets or exceeds 98.0%. Molecular identity is verified using Electrospray Ionization Mass Spectrometry (ESI-MS), confirming exact mass-to-charge ratios match calculated values without structural adducts or missing amino acid sequences.

Furthermore, because bacterial lipopolysaccharides (LPS) disrupt cell culture viability and induce fever responses in animal models, PX1 Research enforces stringent Chromogenic LAL assay limits. Every batch is certified with an endotoxin profile under <0.01 EU/mg. Lab managers can review and download batch-specific documentation directly via our verified COA portal.

Determining Ideal Vial Formatting: Bulk 50mg vs. Standard 2mg/5mg Units

Choosing between high-mass bulk formats (50mg) and single-use operational formats (2mg or 5mg) depends on laboratory throughput, refrigeration infrastructure, and experimental design.

Bulk 50mg vials offer optimized logistics and unit efficiency for continuous, high-volume automated robotic liquid handlers, cell culture media supplementation, or massive rodent cohort studies running simultaneously over a short time frame. However, for intermittent testing or protocols with few subjects, standard 2mg or 5mg vials are generally preferred. Standard vials reduce the window of liquid-state degradation and avoid potential contamination risks associated with repeated handling of large master stocks.

Principal investigators managing multi-project facilities or institutional procurement departments seeking specialized fill sizes, custom batch runs, or scaled account structures can submit direct inquiries through our wholesale lab portal.

Manufacturing Standards and Supply Chain Integrity at PX1 Research

PX1 Research compounds are manufactured inside modern, cGMP-compliant facilities located within the United States. Adherence to strict United States manufacturing guidelines guarantees consistent peptide chain assembly, elimination of residual TFA (trifluoroacetic acid) salts, and complete removal of organic synthesis solvents.

All products are stored in climate-controlled, monitoring-equipped cleanroom facilities before immediate distribution. To preserve compound integrity against heat exposure during transit, PX1 Research provides same-day dispatch for orders confirmed Monday through Friday before cut-off times, shipping directly from specialized fulfillment centers in California and Arizona. Further details regarding GHRH family structures are available in our GHRH analog category hub.