Sermorelin 2mg — Vial Spec, Reconstitution Math & COA
A 2mg vial of Sermorelin provides a precise unit mass of synthetic growth hormone-releasing hormone (GHRH 1-29 amide) engineered for high-precision in vitro assays and preclinical animal models. This technical specification guide details molecular calculations, reconstitution protocols across varied diluent volumes, aliquot stability, and quality control standards. All materials supplied by PX1 Research are strictly designated for laboratory research use only.
Quick answer
A 2mg vial of Sermorelin provides a precise unit mass of synthetic growth hormone-releasing hormone (GHRH 1-29 amide) engineered for high-precision in vitro assays and preclinical animal models. This technical specification guide details molecular calculations, reconstitution protocols across varied diluent volumes, aliquot stability, and quality control standards. All materials supplied by PX1 Research are strictly designated for laboratory research use only.
Key takeaways
- A 2mg vial of [Sermorelin](/research-peptides/sermorelin) contains 2.0 milligrams of sterile, lyophilized Sermorelin acetate powder—a synthetic 29-amino acid peptide representing the functional N-terminal fragment of endogenous human growth hormone-releasing hormone (GHRH 1-44).
- [Sermorelin](/research-peptides/sermorelin) acetate (CAS 86168-78-7) possesses the chemical sequence Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2 and a molecular mass of 3357.9 Da.
- Reconstituting a 2mg (2000 micrograms) lyophilized vial requires precise volumetric calculation based on the desired microgram-per-microliter (µg/µL) concentration for micro-pipetting.
- Repeated freeze-thaw cycles compromise the peptide tertiary structure and accelerate cleavage of delicate amide bonds.
Overview of Sermorelin 2mg Vial Specifications & Lab Applications
A 2mg vial of Sermorelin contains 2.0 milligrams of sterile, lyophilized Sermorelin acetate powder—a synthetic 29-amino acid peptide representing the functional N-terminal fragment of endogenous human growth hormone-releasing hormone (GHRH 1-44). Investigators select a 2mg fill size when conducting medium-throughput receptor binding assays, short-term rodent bioassays, or pulse-dosing studies where maintaining freshness post-reconstitution is critical to experimental reproducibility.
Selecting the 2mg format allows research teams to minimize reagent degradation caused by repeated thermal cycling or extended storage of reconstituted solutions. PX1 Research supplies high-purity Sermorelin acetate manufactured in GMP-compliant, USA-based facilities. Laboratories evaluating secretagogues can review our full catalog of research peptides to compare mass options, purity metrics, and structural specifications.
Molecular Structure, Receptor Binding, and Preclinical Mechanism
Sermorelin acetate (CAS 86168-78-7) possesses the chemical sequence Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2 and a molecular mass of 3357.9 Da. As the shortest fully functional fragment of natural GHRH, it retains complete binding affinity for the growth hormone-releasing hormone receptor (GHRH-R), a transmembrane G-protein coupled receptor located on anterior pituitary somatotrophs.
In vitro data indicate that binding of Sermorelin to GHRH-R stimulates the intracellular adenylate cyclase pathway, elevating cyclic adenosine monophosphate (cAMP) and activating protein kinase A (PKA). This signaling cascade triggers the transcription and exocytosis of growth hormone in cell culture systems. Preclinical studies suggest that because Sermorelin lacks the full 44-amino acid structure, its enzymatic half-life in plasma models is short (typically 10–12 minutes), making it an ideal candidate for studying physiological somatotroph pulsatility without inducing receptor downregulation.
Reconstitution Math: Diluent Volume & Mass-Concentration Ratios
Reconstituting a 2mg (2000 micrograms) lyophilized vial requires precise volumetric calculation based on the desired microgram-per-microliter (µg/µL) concentration for micro-pipetting. Standard laboratory diluents include bacteriostatic water (0.9% benzyl alcohol) for multi-use analytical samplings or sterile 0.9% sodium chloride (saline) for immediate cell culture application.
When adding 1.0 mL of diluent to a 2mg vial, the resulting concentration is 2.0 mg/mL (2000 µg/mL or 2.0 µg/µL). Reconstitution with 2.0 mL of diluent produces a concentration of 1.0 mg/mL (1000 µg/mL or 1.0 µg/µL), which simplifies aliquot dosing in animal bioassays. Adding 3.0 mL of diluent yields a final concentration of approximately 0.667 mg/mL (666.7 µg/mL or 0.667 µg/µL). To streamline bench calculations and verify liquid displacement ratios across various vial sizes, researchers rely on our interactive reconstitution calculator.
Aliquot Planning and Serial Dilution Workflows for Assays
Repeated freeze-thaw cycles compromise the peptide tertiary structure and accelerate cleavage of delicate amide bonds. To maintain compound integrity post-reconstitution, laboratory protocols dictate immediate subdivision of the reconstituted 2mg stock solution into single-use, low-binding polypropylene microcentrifuge tubes.
For receptor binding assays or cell perfusion experiments requiring nanomolar (nM) working solutions, serial dilutions should be prepared immediately prior to testing. Stock aliquots (e.g., 50 µL to 100 µL) stored at -20°C or -80°C maintain high stability. When preparing working solutions in phosphate-buffered saline (PBS) or cell culture media, researchers should account for non-specific surface adsorption by using carrier proteins (such as 0.1% bovine serum albumin) if ultra-low concentrations (<10 nM) are required.
Comparative Analysis: Sermorelin vs. CJC-1295, Ipamorelin, and Tesamorelin
In preclinical secretagogue research, investigators frequently compare Sermorelin to other GHRH analogs and ghrelin receptor agonists. Unlike CJC-1295 without DAC, which contains four substituted amino acids designed to resist dipeptidyl peptidase-IV (DPP-IV) enzymatic degradation, native Sermorelin undergoes rapid cleavage in rodent plasma models, making it preferable for pulse-simulation studies. Contrastingly, Ipamorelin is a selective agonist of the growth hormone secretagogue receptor (GHS-R1a) rather than the GHRH-R, activating GH secretion via the phospholipase C pathway rather than the cAMP pathway.
Another relevant compound, Tesamorelin, incorporates a trans-3-hexenoic acid group at the N-terminus, extending its systemic stability while preserving GHRH-R selectivity. Researchers evaluating these distinct pharmacokinetic profile differences across the GHRH class analogs can review mechanistic data and comparative research literature in the PX1 peptide research library.
Quality Control: Lot-Matched COA, HPLC, MS, and Endotoxin Verification
To ensure rigor and reproducibility across preclinical trials, PX1 Research subjects every batch of Sermorelin 2mg to rigorous analytical validation at an independent ISO 17025 accredited testing laboratory. Physical purity is confirmed via High-Performance Liquid Chromatography (HPLC), guaranteeing chemical purity exceeding 98.0%.
Mass Spectrometry (MS) electrospray analysis confirms exact peptide identity by matching observed mass to theoretical weight (3357.9 Da). Furthermore, because bacterial endotoxins interfere with somatotroph receptor assays and induce inflammatory responses in animal models, every lot undergoes Limulus Amebocyte Lysate (LAL) testing to confirm endotoxin levels remain strictly below <0.01 EU/mg. Investigators can inspect and download the exact lot-matched Certificate of Analysis (COA) prior to order placement.
Storage Conditions, Freeze-Thaw Limits, and Degradation Pathways
Lyophilized Sermorelin 2mg vials must be stored in a controlled freezer environment at -20°C or -80°C, protected from light exposure and ambient moisture. Under dry frozen conditions, the un-reconstituted peptide retains structural integrity for up to 24 months from the manufacturing date.
Following reconstitution with bacteriostatic water, liquid stock solutions remain stable at 2°C to 8°C for 14 to 28 days. Avoid ultrasonic bath agitation or vigorous mechanical shaking during dissolution, as liquid shear stress can cause peptide aggregation and loss of bioactive mass. Unused reconstituted portions intended for long-term storage must be frozen immediately into single-use aliquots at -80°C and thawed only once prior to assay incorporation.
Vial Size Selection: Evaluating 2mg vs. 5mg and Bulk Institutional Formulations
Selecting the ideal vial unit mass depends entirely on study scale, sample sizing, and operational timeline. The 2mg vial size is optimal for acute dosage-response curves, pilot microplate assays, or short-term rodent cohort studies where reconstituted stock solutions will be fully exhausted within 1 to 2 weeks, eliminating product waste.
For long-term longitudinal studies, high-throughput automated platforms, or multi-animal cohorts requiring larger material volumes, purchasing larger mass options (such as 5mg or 10mg vials) or arranging continuous supply through PX1's program for bulk institutional orders provides greater operational efficiency and batch consistency. All orders ship same-day from CA and AZ fulfillment centers when placed Monday through Friday before cut-off times.
Frequently Asked Questions
What is the exact mass contained in a Sermorelin 2mg vial?
A 2mg vial contains precisely 2.0 milligrams (2000 micrograms) of lyophilized Sermorelin acetate powder, analytical purity verified to >98.0%.
How much diluent should be added to a 2mg vial of Sermorelin for lab assays?
Adding 1.0 mL of diluent yields a 2.0 mg/mL (2000 µg/mL) concentration. Adding 2.0 mL yields a 1.0 mg/mL (1000 µg/mL) concentration. The optimal volume depends on target micro-pipetting volumes for your specific assay protocol.
Does PX1 Research provide lot-specific COAs for 2mg vials?
Yes. Every fill size and batch is assigned a unique lot number with a corresponding COA detailing HPLC purity, mass spectrometry weight confirmation, and LAL endotoxin testing.
Can reconstituted Sermorelin 2mg be refrozen multiple times?
No. Repeated freeze-thaw cycles cause physical denaturation and degradation of the peptide sequence. Reconstituted stock should be aliquoted into single-use containers before frozen storage at -20°C or -80°C.
What is the target receptor for Sermorelin in cell culture research?
Sermorelin selectively binds to the growth hormone-releasing hormone receptor (GHRH-R) on anterior pituitary somatotrophs, activating the adenylate cyclase / cAMP intracellular pathway.
How long is lyophilized Sermorelin 2mg stable at freezer temperatures?
Unopened, lyophilized Sermorelin stored at -20°C or -80°C remains structurally stable and fully potent for up to 24 months.
What are the endotoxin limits for PX1 Research peptides?
PX1 Research verifies that endotoxin levels in Sermorelin remain strictly below <0.01 EU/mg via LAL assay testing to prevent cell culture or bioassay interference.
Where are PX1 Research peptides manufactured and shipped from?
All PX1 Research compounds are USA-manufactured in GMP-compliant facilities and shipped directly from fulfillment centers located in California and Arizona with same-day shipping on weekday orders.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.