MOTS-C 60mg — Vial Spec, Reconstitution Math & COA

Overview of MOTS-C 60mg Vial Specifications

A 60mg vial of MOTS-C contains 60 milligrams of lyophilized, synthetic 16-amino-acid peptide (sequence: Met-R-Q-R-L-F-I-L-L-R-L-L-E-K-L-N) stabilized as a trifluoroacetate (TFA) or acetate salt. High-mass vial configurations such as 60mg are primarily sourced by institutional research laboratories running high-throughput in vitro screens, multi-plate enzyme assays, or extended animal model cohorts. Utilizing a single large-format vial reduces inter-vial variability across large assay batches, ensuring uniform baseline concentrations across experimental plates.

In preclinical settings, MOTS-C is classified strictly as a mitochondrial research compound. Preclinical data indicate that MOTS-C translocates to the nucleus under metabolic stress conditions, where it interacts with nuclear transcription factors to regulate metabolic genes, glucose uptake, and fatty acid oxidation. Obtaining accurate volumetric concentrations during dilution is essential for reproducibility across cell culture assays and animal models studying exercise capacity.

PX1 Research Product Availability Notice

Please note that PX1 Research distributes MOTS-C primarily in standard analytical unit sizes (such as 5mg and 10mg vials) optimized for standard benchtop protocols and minimal freeze-thaw exposure. While a 60mg single-vial unit is a specialized format used for automated robotics platforms and large-scale synthesis requests, laboratories requiring this total mass can review our standard MOTS-C product page or explore our complete catalog of research peptides to aggregate equivalent mass across smaller, highly stable single-use vials.

For custom bulk orders, high-volume fill requests, or enterprise lab accounts requiring specialized mass configurations like 60mg per vial, institutional buyers can coordinate directly with our procurement team via our wholesale portal. All formulations, regardless of fill mass, adhere to identical analytical standards, including ISO 17025 HPLC purity testing and mass spectrometry confirmation.

Reconstitution Math for a 60mg MOTS-C Vial

Reconstituting a 60mg lyophilized cake requires precise volumetric calculation based on the desired working concentration and solvent properties. MOTS-C is typically reconstituted using Bacteriostatic Water (0.9% benzyl alcohol) for multi-use laboratory bench work or Sterile 0.9% Sodium Chloride / Phosphate-Buffered Saline (PBS) for acute cell culture media preparations.

Below are the exact concentration yields when dissolving a 60mg MOTS-C vial in standard diluent volumes:

1.0 mL Diluent Addition: Yields a concentration of 60.0 mg/mL (60.0 µg/µL). This creates a highly concentrated stock solution ideal for high-density aliquoting or micro-volume automated dispensing systems.

2.0 mL Diluent Addition: Yields a concentration of 30.0 mg/mL (30.0 µg/µL). This standard dilution balances volume precision with pipetting accuracy for working assays.

3.0 mL Diluent Addition: Yields a concentration of 20.0 mg/mL (20.0 µg/µL). This lower-viscosity solution is suited for manual multi-channel pipetting across 96-well culture plates.

To quickly recalculate custom working volumes or target unit concentrations for different assay formats, researchers should utilize our interactive reconstitution calculator.

Aliquot Planning & Freeze-Thaw Prevention

Peptide degradation in aqueous solution occurs primarily through hydrolysis, oxidation, and secondary structure aggregation. Reconstituting a 60mg vial generates a large volume of active solution; subjecting this entire stock to repeated freeze-thaw cycles significantly compromises the structural integrity of the peptide sequence.

To preserve peptide potency, laboratories should implement an immediate aliquoting protocol post-reconstitution. Dissolve the 60mg lyophilized powder completely by gentle swirling (never vigorous vortexing, which induces mechanical shear stress). Using sterile, polypropylene low-binding microcentrifuge tubes, subdivide the primary stock into single-use working aliquots based on daily protocol consumption (e.g., 50 µL to 100 µL volumes).

Once aliquoted, immediately flash-freeze working tubes in liquid nitrogen or an ethanol/dry-ice bath prior to transfer into long-term ultra-low storage. Working tubes should be thawed only once prior to addition to culture media or experimental assay mixtures.

Storage Parameters and Temperature Stability

Lyophilized Powder: Unreconstituted 60mg MOTS-C vials must be stored at -20°C for short-to-medium term storage (1 to 6 months) or at -80°C for extended stability (up to 24 months). Store vials in a desiccated environment protected from direct light exposure.

Reconstituted Liquid Stock: Once reconstituted, liquid stock solutions should be maintained at 2°C to 8°C if they will be completely consumed within 48 to 72 hours. For longer storage intervals, reconstituted aliquots must be frozen at -20°C or -80°C. Avoid frost-free freezers, as their automated temperature cycling introduces destructive micro-thaw events.

Quality Assurance: ISO 17025 COA & Analytical Verification

High-yield research protocols require verifiable purity to prevent false-positive metabolic readings caused by truncated sequence fragments or synthesis byproducts. Every lot manufactured for PX1 Research undergoes strict analytical verification prior to release.

Our analytical validation protocol includes High-Performance Liquid Chromatography (HPLC) to confirm peptide purity exceeds 98.0%, combined with Liquid Chromatography-Mass Spectrometry (LC-MS) to confirm exact molecular weight match (1874.3 g/mol). Furthermore, endotoxin testing via Chromogenic LAL assay ensures bacterial endotoxin levels remain below rigorous thresholds (<0.01 EU/mg), making the compound suitable for sensitive in vitro enzymatic screens and cell culture models. Investigators can inspect lot-matched documentation directly on our dedicated COA verification page.

Comparative Analysis: Mitochondrial Research Compounds

MOTS-C belongs to an expanding class of mitochondrial-targeted peptides and mitochondrial-derived peptides (MDPs) evaluated for metabolic and physical capacity modulation. To select the correct agent for target-specific pathways, researchers frequently compare MOTS-C against other mitochondrial regulators:

In preclinical models, MOTS-C acts primarily via the AMPK pathway to regulate systemic metabolic homeostasis and skeletal muscle insulin sensitivity. In contrast, compounds like SS-31 (Elamipretide) target the inner mitochondrial membrane directly by binding cardiolipin to reduce electron transport chain ROS leakage without primary transcriptional regulation. Meanwhile, Humanin targets cytoprotective pathways and anti-apoptotic cascades under oxidative stress conditions. Review our complete mitochondrial peptides research library for comprehensive mechanism comparisons across these related compounds.

Determining Optimal Vial Size: 60mg vs. Standard 5mg/10mg Fill Sizes

Selecting between a 60mg high-capacity vial and standard smaller unit sizes depends entirely on experimental design and consumption velocity:

60mg High-Capacity Vials: Best suited for automated high-throughput screening (HTS) laboratories, large-scale animal study cohorts requiring uniform single-lot dosing, or continuous bioreactor additions where full reconstitution occurs immediately prior to high-volume usage.

5mg or 10mg Standard Vials: Best suited for pilot studies, exploratory in vitro assays, or multi-phase projects spread across extended timelines. Smaller fill sizes limit the risk of accidental room-temperature exposure, contamination, or freeze-thaw degradation inherent to opening large primary stocks.

For additional technical documentation on experimental design, compound stability, and analytical chemistry protocols, explore our overarching research hub.