5-Amino-1MQ 15mg — Vial Spec, Reconstitution Math & COA

Overview of 5-Amino-1MQ 15mg Formulations and Lab Utilization

The 15mg vial specification of 5-Amino-1MQ represents an expanded mass format tailored for academic and clinical research laboratories conducting multi-well microplate screens or longitudinal animal models. 5-Amino-1MQ is a small-molecule membrane-permeable derivative of methylquinolinium that acts as a selective, membrane-permeable inhibitor of nicotinamide N-methyltransferase (NNMT). In biochemical research, NNMT plays a pivotal regulatory role in cellular energy metabolism, nutrient sensing, and epigenetic modification by methylating nicotinamide using S-adenosylmethionine (SAM) as a methyl donor.

Laboratories select a 15mg vial size primarily when running high-density assay formats—such as 96-well or 384-well cell culture assays—where consistent batching is required to eliminate vial-to-vial variability across experimental replicates. A single 15mg unit yields sufficient material to prepare broad concentration gradients (e.g., nanomolar to micromolar ranges) or to dose complete rodent cohorts throughout multi-week metabolic studies without switching manufacturing lots. All research compounds supplied in this format are intended exclusively for in vitro and preclinical research applications in controlled laboratory settings.

PX1 Product Availability and Format Selection

PX1 Research manufactures and packages high-purity research compounds under strict quality management systems. Researchers seeking standard low-volume or initial pilot-scale quantities should note that PX1 maintains the 5-Amino-1MQ 5mg vial as a core catalog item. The 5mg presentation is often ideal for preliminary baseline studies, solubility checks, or limited cell culture runs where reconstituted material must be consumed rapidly to prevent aqueous hydrolysis.

For institutions requiring larger mass quantities such as the 15mg specification, PX1 provides specialized custom filling and bulk fulfillment options. Researchers can explore the full catalog via our all peptides inventory or contact our institutional account managers through our wholesale portal to arrange bulk lot runs, custom vial fills, or specialized packaging protocols tailored to specific experimental pipelines. Regardless of the fill mass, every lot adheres to the same stringent analytical purity metrics.

Reconstitution Math and Concentration Benchmarks (1mL, 2mL, 3mL)

Accurate reconstitution of 15mg 5-Amino-1MQ requires precise volumetric solvent addition to achieve target mass concentrations and molarities. Because 5-Amino-1MQ has a molecular weight of approximately 160.22 g/mol (as a free base, varying slightly depending on salt form specifications), calculations must account for total diluent volume added to the sealed vial under aseptic conditions.

When reconstituting a 15mg mass with 1.0 mL of laboratory-grade diluent (such as sterile bacteriostatic water, phosphate-buffered saline, or a minimal percentage DMSO co-solvent depending on assay compatibility), the resulting stock concentration is precisely 15.0 mg/mL (15.0 µg/µL). For a 150 µM target working concentration in cell culture media, researchers dilute this stock using standard volumetric pipetting equations ($C_1V_1 = C_2V_2$). To streamline these multi-step dilutions, researchers frequently utilize our interactive reconstitution calculator to determine exact micro-litre additions.

If 2.0 mL of diluent is introduced to the 15mg vial, the final stock concentration shifts to 7.5 mg/mL (7.5 µg/µL). This mid-range concentration is frequently chosen for animal dosing protocols where syringe fluid volume limits necessitate a balance between solubility limits and precise volumetric transfer. Adding 3.0 mL of diluent produces a concentration of 5.0 mg/mL (5.0 µg/µL), which is well-suited for high-volume serial dilution schemes across microplate columns where smaller mass margins minimize pipetting error.

Aliquot Planning, Handling, and Working Solution Protocols

Repeated freeze-thaw cycles significantly accelerate the degradation of organic compounds and small-molecule inhibitors in aqueous solution. Upon initial reconstitution of a 15mg vial, researchers should immediately divide the stock solution into single-use micro-aliquots using sterile, low-binding polypropylene microcentrifuge tubes. Aliquot volumes should be calculated based on the maximum daily consumption requirement of the experimental protocol.

Working solutions prepared in aqueous buffers should be used immediately or maintained at 2°C to 8°C for no longer than 24–48 hours depending on the buffer pH and organic co-solvent percentage. When handling 5-Amino-1MQ, exposure to ambient light and thermal spikes should be minimized. Working under a laminar flow hood with amber or light-blocking storage vials prevents light-induced photo-degradation during extended assay runs.

Mechanism of Action: NNMT Inhibition and Cellular Energetics

In biochemical literature, 5-Amino-1MQ is documented as a small-molecule, selective inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme predominantly expressed in adipose tissue, liver, and specific cancer cell lines, where it catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), generating 1-methylnicotinamide (MNA) and S-adenosylhomocysteine (SAH). High NNMT activity depletes cellular NAD+ pools and alters the SAM/SAH ratio, impacting both cellular respiration and chromatin methylation dynamics.

Preclinical studies suggest that targeted inhibition of NNMT by 5-Amino-1MQ reduces MNA production, effectively conserving intracellular nicotinamide for salvage pathway conversion into NAD+. In vitro data indicate that elevated intracellular NAD+ concentrations promote enhanced mitochondrial respiration, increased SIRT1 enzyme activation, and improved cellular metabolic flux. Consequently, 5-Amino-1MQ serves as an essential research probe in investigation focused on lipid metabolism, adipocyte differentiation, mitochondrial biogenesis, and age-related metabolic dysfunction.

Comparative Analysis: Metabolic Research Compounds

In metabolic and mitochondrial research pipelines, investigators frequently compare 5-Amino-1MQ against other candidate molecules that influence cellular bioenergetics through distinct molecular pathways. While 5-Amino-1MQ operates specifically through cytosolic NNMT inhibition to preserve NAD+ precursor pools, alternative compounds target mitochondrial transcript translation or lipolytic signal cascades.

For instance, researchers studying mitochondrial-derived peptides often evaluate MOTS-c, a mitochondrial-encoded peptide that promotes metabolic homeostasis and AMPK activation during metabolic stress. Similarly, fragments derived from human growth hormone pathways, such as AOD-9604, are investigated for their direct lipolytic activity in isolated adipocytes without inhibiting methyltransferase enzymes. Integrating 5-Amino-1MQ alongside these pathway-specific reagents allows investigators to map non-overlapping metabolic targets within complex cellular energy networks. Additional literature and comparative study designs can be reviewed in the PX1 research library.

Analytical Quality Assurance: HPLC, MS, and Endotoxin Standards

To ensure reproducible assay results, every batch of 5-Amino-1MQ produced for PX1 Research undergoes rigorous multi-tier analytical testing. Quality verification is conducted in independent ISO 17025 accredited laboratories using High-Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry (MS). HPLC analysis confirms chemical purity levels exceeding 98.0%, ensuring that trace synthesis intermediates or degradation products do not confound biological assays.

Mass spectrometry confirms the precise molecular weight and structural identity of the synthesized compound. Furthermore, because bacterial lipopolysaccharides can stimulate inflammatory pathways in cell cultures and animal models—thereby skewing metabolic data—PX1 subjects every lot to chromogenic Limulus Amebocyte Lysate (LAL) testing to confirm endotoxin levels remain strictly below <0.01 EU/µg. Investigators can download lot-specific analytical documentation directly via our COA portal.

Storage Guidelines and Hydrolytic Stability

Proper storage conditions are critical for preserving the chemical integrity of 5-Amino-1MQ before and after reconstitution. Lyophilized powder in sealed manufacturer vials should be stored at -20°C for short-to-medium term storage (up to 12 months) or at -80°C for extended archival preservation. Vials should be kept in a desiccated container protected from atmospheric moisture and direct light exposure.

Before opening a cold vial to introduce diluent, allow the vial to equilibrate to room temperature (approximately 20°C to 25°C) for at least 30 minutes. This equilibration step prevents ambient condensation from forming inside the vial, which can lead to premature hydrolysis or localized concentration errors during mass measurement. Reconstituted aliquots stored at -80°C maintain chemical stability for up to 3 to 6 months, provided freeze-thaw cycles are strictly avoided.