5-Amino-1MQ 50mg — Vial Spec, Reconstitution Math & COA
A 50mg vial of 5-Amino-1MQ provides high-capacity yield for laboratories conducting high-throughput in vitro screens or extended preclinical rodent studies. Designed for researchers investigating metabolic regulation, this specialized vial quantity offers consistent active mass for precise liquid handling and concentration modeling. This guide details the chemical specifications, dilution math, storage requirements, and batch quality protocols required for rigorous laboratory investigations.
Quick answer
A 50mg vial of 5-Amino-1MQ provides high-capacity yield for laboratories conducting high-throughput in vitro screens or extended preclinical rodent studies. Designed for researchers investigating metabolic regulation, this specialized vial quantity offers consistent active mass for precise liquid handling and concentration modeling. This guide details the chemical specifications, dilution math, storage requirements, and batch quality protocols required for rigorous laboratory investigations.
Key takeaways
- The 50mg vial format of [5-Amino-1MQ](/research-peptides/5-amino-1mq) is engineered specifically for academic, institutional, and industrial research laboratories that require larger material reserves for high-volume experimental setups.
- Please note: PX1 Research maintains flexible inventory based on institutional demand.
- [5-Amino-1MQ](/research-peptides/5-amino-1mq) acts as a selective, membrane-permeable small molecule inhibitor of nicotinamide N-methyltransferase (NNMT).
- Accurate reconstitution is vital for maintaining reproducible experimental conditions.
Overview of the 5-Amino-1MQ 50mg Vial Format
The 50mg vial format of 5-Amino-1MQ is engineered specifically for academic, institutional, and industrial research laboratories that require larger material reserves for high-volume experimental setups. Containing 50 milligrams of highly purified 5-amino-1-methylquinolinium salt, this specification eliminates the variable errors associated with reconstituting multiple low-dose vials during a single assay series.
Principal investigators and laboratory technicians typically select the 50mg vial when executing multi-well plate assays, high-throughput enzymatic screens, or chronic dosing protocols in animal models where batch-to-batch consistency is essential. By consolidating material into a single standardized vial, research teams minimize mass transfer loss, streamline reconstitution procedures, and maintain a constant reagent stock across extended experimental timelines.
Catalog Availability & High-Capacity Sourcing Notice
Please note: PX1 Research maintains flexible inventory based on institutional demand. While our standard catalog prominently features smaller single-assay units such as our 5-Amino-1MQ 5mg format for baseline testing, custom high-capacity fills—including 50mg vials—are routinely processed for accredited institutions through our wholesale lab program.
If a 50mg unit is currently out of stock on the primary retail storefront, investigators can review our complete catalog of all peptides or request a custom fill size through our laboratory support desk. All custom bulk fills undergo the exact same ISO 17025 analytical validation and high-performance liquid chromatography (HPLC) purification process as our standard inventory items.
Molecular Mechanism: NNMT Inhibition and Cellular Energetics
5-Amino-1MQ acts as a selective, membrane-permeable small molecule inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme responsible for catalyzing the methylation of nicotinamide (NAM) using S-adenosylmethionine (SAM) as the methyl donor, producing 1-methylnicotinamide (MNA) and S-adenosylhomocysteine (SAH). In metabolic tissue models, elevated NNMT activity depletes intracellular pools of nicotinamide, thereby restricting the salvage pathway for nicotinamide adenine dinucleotide (NAD+) synthesis.
Preclinical studies suggest that targeted inhibition of NNMT by 5-Amino-1MQ prevents the methylation and excretion of nicotinamide. In vitro assays demonstrate that preserving intracellular nicotinamide concentrations supports the salvage pathway, subsequently raising NAD+ levels within adipocytes and myocytes. Furthermore, animal study models indicate that this enhancement in cellular NAD+ availability promotes mitochondrial biogenesis, optimizes electron transport chain efficiency, and drives oxygen consumption rates. Consequently, 5-Amino-1MQ remains a pivotal tool in modern fat-metabolism research and mitochondrial output studies.
50mg Reconstitution Math: Dilution Guidelines for Laboratory Use
Accurate reconstitution is vital for maintaining reproducible experimental conditions. Because 50mg represents a relatively large mass of active compound, selecting the correct diluent volume determines both final stock concentration and the solubility profile of the solution. Reconstitution should always be performed under a laminar flow hood using sterile, laboratory-grade solvents such as Bacteriostatic Water, Phosphate-Buffered Saline (PBS), or dimethyl sulfoxide (DMSO), depending on assay parameters.
To achieve target working concentrations from a 50mg vial, researchers can utilize the following standard dilution ratios: adding 1.0 mL of diluent yields a highly concentrated stock of 50.0 mg/mL (50.0 µg/µL); adding 2.0 mL yields a concentration of 25.0 mg/mL (25.0 µg/µL); and adding 3.0 mL yields 16.67 mg/mL (16.67 µg/µL). For rapid verification of custom volumes and sub-milligram concentrations, investigators should consult our interactive reconstitution calculator prior to solvent introduction.
Aliquot Planning and Degradation Prevention Strategies
Once a 50mg vial of 5-Amino-1MQ is fully solubilized, exposing the stock solution to repeated freeze-thaw cycles can induce chemical degradation, precipitation, or hydrolysis over time. To preserve molecular integrity across long-term studies, research teams must implement an organized aliquot strategy immediately following initial reconstitution.
Working under sterile conditions, the reconstituted stock solution should be partitioned into single-use, low-binding polypropylene microcentrifuge tubes. Aliquot volumes should be calculated to match the exact requirements of a single experimental run (e.g., 50 µL to 200 µL per tube). Tubes must be clearly labeled with the compound name, lot number, concentration, and reconstitution date before being snap-frozen in liquid nitrogen or an ultra-low temperature freezer set to -80°C.
Comparative Analysis: 5-Amino-1MQ and Related Metabolic Compounds
When designing protocols focused on cellular metabolism, mitochondrial density, and energy homeostasis, researchers frequently compare 5-Amino-1MQ against other candidate agents within our research library. Understanding the unique pathways targeted by different compounds enables investigators to construct effective comparative or combination in vitro models.
While 5-Amino-1MQ acts specifically via cytosolic NNMT inhibition to preserve NAD+ precursor availability, mitochondrial-derived peptides operate through distinct organelle-level mechanisms. For instance, MOTS-c targets nuclear gene expression to regulate folate cycles and metabolic homeostasis, whereas SS-31 binds directly to cardiolipin in the inner mitochondrial membrane to reduce electron leakage and reactive oxygen species (ROS) production. Additionally, growth hormone secretagogues like Tesamorelin modulate systemic lipolysis through central endocrine signaling rather than direct intracellular enzyme inhibition.
Analytical Rigor: COA Verification and Quality Assurance
PX1 Research enforces strict quality control standards for every production batch. Prior to dispensing into 50mg or smaller vial formats, every lot of 5-Amino-1MQ undergoes rigorous testing at an independent ISO 17025 accredited laboratory to verify absolute identity, purity, and mass balance.
Analytical methods include High-Performance Liquid Chromatography (HPLC) to confirm purity exceeding 98.0%, and Electrospray Ionization Mass Spectrometry (ESI-MS) to verify precise molecular weight. Furthermore, bacterial endotoxin testing (Chromogenic LAL assay) is conducted to ensure endotoxin levels remain well below standard cell-culture threshold limits. Investigators can view or download lot-specific analytical reports directly through our dedicated COA verification portal.
Storage Protocols and Lyophilized Powder Stability
Unreconstituted, lyophilized 5-Amino-1MQ 50mg vials must be stored in temperature-controlled environments to prevent premature degradation. Upon receipt, sealed vials should be kept in a desiccated freezer at -20°C for short-to-medium term storage, or at -80°C for extended archival preservation. Under these conditions, the dry compound maintains its chemical stability and structural integrity for up to 24 months.
Vials must be protected from ambient light and moisture ingress. Prior to unsealing and introducing solvents, allow the lyophilized vial to equilibrate to room temperature for approximately 20 to 30 minutes. This thermal equilibration step prevents atmospheric moisture from condensing inside the vial, which could impair precise weighing or alter solvent concentrations during initial dissolution.
Selecting the Optimal Specification: 50mg vs. Micro-Quantities
Choosing between a single 50mg vial and multiple micro-quantity vials (such as 5mg options) depends on the scale, duration, and budget of the experimental protocol. High-throughput screening platforms and rodent model studies consuming high daily mass benefit substantially from the cost efficiency and batch uniformity of a 50mg unit.
Conversely, laboratories conducting pilot studies, exploratory in vitro assays, or infrequent testing schedules may prefer smaller 5mg vials to minimize waste and avoid storing reconstituted liquid stocks over prolonged intervals. PX1 Research supports both research scales, supplying uniform, USA-manufactured reagents across all catalog specifications.
Frequently Asked Questions
What is the physical state and target application of 5-Amino-1MQ 50mg?
5-Amino-1MQ 50mg is supplied as a lyophilized (freeze-dried) cake or powder in a sealed glass vial. It is designed strictly for laboratory research use in vitro or in animal models to study NNMT inhibition, cellular energy dynamics, and NAD+ metabolism.
What is the concentration of a 50mg vial reconstituted with 2mL of solvent?
Reconstituting a 50mg vial with 2.0 mL of diluent produces a stock concentration of 25.0 mg/mL (25.0 µg/µL).
Which solvents are recommended for reconstituting 5-Amino-1MQ?
Depending on the downstream assay, common solvents include sterile Bacteriostatic Water, Phosphate-Buffered Saline (PBS), or laboratory-grade DMSO. Solubilization characteristics should be verified based on target cell culture or assay tolerances.
How is the purity of PX1 Research 5-Amino-1MQ verified?
Every batch undergoes third-party ISO 17025 laboratory verification using HPLC to confirm >98% purity and Mass Spectrometry (MS) to verify chemical identity. Certificates of Analysis (COAs) are issued per lot.
Can reconstituted 5-Amino-1MQ be subjected to repeated freeze-thaw cycles?
No. Repeated freeze-thaw cycles cause physical and chemical degradation. Reconstituted stock solutions should be partitioned into single-use aliquots and frozen at -20°C or -80°C.
What is the shelf life of 5-Amino-1MQ in lyophilized form?
When stored in a desiccated freezer at -20°C to -80°C away from light, lyophilized 5-Amino-1MQ retains stability for up to 24 months.
Is 5-Amino-1MQ suitable for human or veterinary administration?
No. All products provided by PX1 Research are strictly for laboratory research use only. They are not intended for human, clinical, therapeutic, or veterinary applications.
What if PX1 Research currently shows 50mg out of stock on the website?
If the 50mg specification is not immediately listed for direct online checkout, researchers can purchase multiple 5mg vials or contact our support team to request a custom high-capacity fill through our wholesale program.
Related pages
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.