PX1 Research provides laboratory-grade Chonluten (Glu-Asp-Gly) for in vitro and preclinical investigation into respiratory biology and cellular stress pathways. As a premier USA-based supplier, we furnish analytical-grade bioregulatory peptides accompanied by lot-specific certificates of analysis, verification by RP-HPLC and mass spectrometry, and strict endotoxin quantification.
PX1 Research provides laboratory-grade Chonluten (Glu-Asp-Gly) for in vitro and preclinical investigation into respiratory biology and cellular stress pathways. As a premier USA-based supplier, we furnish analytical-grade bioregulatory peptides accompanied by lot-specific certificates of analysis, verification by RP-HPLC and mass spectrometry, and strict endotoxin quantification.
PX1 Research is a dedicated USA-based supplier of high-purity Chonluten for laboratory research into antioxidant enzyme regulation. Chonluten (L-glutamyl-L-aspartyl-L-glycine) is a short-chain bioregulatory tripeptide evaluated in preclinical models for its capacity to modulate endogenous antioxidant defense systems, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) within epithelial and pulmonary tissues.
Researchers investigating oxidative stress, cellular homeostasis, and tissue-specific gene expression require high-purity reagents to ensure reproducible experimental outcomes. PX1 Research manufactures Chonluten in GMP-compliant facilities within the United States, validating every batch through ISO 17025 accredited third-party laboratories to deliver verified identity, purity exceeding 98% by RP-HPLC, and minimal endotoxin levels.
Chonluten is a synthetic tripeptide comprising the amino acid sequence L-glutamyl-L-aspartyl-L-glycine (H-Glu-Asp-Gly-OH). Classified within the broader family of short-chain peptide bioregulators, its low molecular mass allows for specific molecular interactions with chromatin and nuclear proteins in cellular assays.
In laboratory settings, researchers examine how ultra-short amino acid sequences penetrate cell membranes and interact with gene promoter regions. To explore our complete catalog of target-specific bioregulators, investigators can access our all research peptides collection for comprehensive technical data.
The carboxyl and side-chain functional groups of the glutamyl and aspartyl residues impart a net negative charge at physiological pH. This unique electrostatic profile is hypothesized to influence histone binding affinity, chromatin unwinding, and subsequent transcriptional activation of cytoprotective gene clusters.
Preclinical studies suggest that Chonluten exerts its biological effects by modulating nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways and directly influencing gene expression associated with antioxidant defense mechanisms. In rodent models of pulmonary oxidative challenge, administration of short-chain peptides has been correlated with upregulation of transcription factors controlling primary enzymatic cascades.
Primary antioxidant enzymes regulated in these assays include:
• Superoxide Dismutase (SOD): Converts reactive superoxide radicals into hydrogen peroxide and molecular oxygen, forming the first enzymatic defense against oxidative damage. • Catalase (CAT): Catalyzes the decomposition of hydrogen peroxide into water and oxygen, protecting cellular structures from lipid peroxidation. • Glutathione Peroxidase (GPx): Reduces lipid hydroperoxides and free hydrogen peroxide through the oxidation of reduced glutathione (GSH).
In vitro data indicate that exposure to the Chonluten peptide leads to statistically significant increases in total antioxidant capacity (TAC) within primary bronchial epithelial cell cultures. By normalizing endogenous enzymatic activities, the peptide limits secondary inflammation markers and preserves cellular membrane integrity during simulated oxidative insult.
Published literature focusing on short-chain bioregulatory peptides highlights Chonluten's specific tropism toward bronchial and pulmonary tissue. In vitro assays using human airway epithelial cells exposed to toxic agents demonstrate that treatment with Glu-Asp-Gly helps maintain intracellular glutathione ratios (GSH/GSSG) and reduces reactive oxygen species (ROS) accumulation.
Animal studies involving acute lung injury and environmental toxin exposure indicate that pre-incubation or co-administration with Chonluten preserves alveolar architecture, suppresses pro-inflammatory cytokine expression (such as TNF-alpha and IL-6), and attenuates neutrophil infiltration into the bronchoalveolar space.
These findings position Chonluten as an essential tool for laboratories studying chronic respiratory conditions, environmental toxicology, and peptide-mediated genomic regulation. For deeper analysis of peptide interactions in cellular systems, review our published articles within the peptide analytical testing hub.
When evaluating synthetic short-chain peptides for tissue-specific antioxidant and homeostatic studies, researchers frequently compare Chonluten with other short-chain sequences targeting epithelial and systemic pathways. Understanding the distinct structural features and cellular tropisms of these peptides allows investigators to construct targeted experimental protocols.
A comparison of key bioregulatory research compounds highlights distinct tissue targets and primary endpoints:
• Chonluten (Glu-Asp-Gly): Specifically targets bronchial and respiratory epithelial cells, primarily modulating SOD, CAT, and Nrf2 expression in lung tissue assays. • Bronchogen peptide (Ala-Glu-Asp-Leu): A synthetic tetrapeptide evaluated for bronchial remodeling and epithelial repair, often studied alongside Chonluten in comparative respiratory models. • Epitalon (Ala-Glu-Asp-Gly): A systemic pineal-derived tetrapeptide that modulates telomerase expression and systemic antioxidant activity rather than tissue-localized respiratory pathways.
Researchers can learn more about general peptide regulation mechanisms in our comprehensive guide to bioregulatory peptide mechanisms.
To guarantee reproducible laboratory data, researchers require strict quality control metrics for incoming synthetic peptides. PX1 Research subjects every lot of Chonluten to rigorous analytical validation before release.
Purity assessment is performed via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) utilizing C18 column chemistry and gradient elution. Every lot must exhibit a chromatographic purity of equal to or greater than 98.0%, eliminating trace peptide impurities or truncated sequences that could skew receptor binding and gene expression assays.
Identity verification is confirmed via Electrospray Ionization Mass Spectrometry (ESI-MS), verifying the observed molecular weight against the theoretical molecular mass of Glu-Asp-Gly (319.27 g/mol). Furthermore, because endotoxins (lipopolysaccharides) alter immune responses in cellular assays, PX1 Research measures endotoxin content using Limulus Amebocyte Lysate (LAL) testing, guaranteeing levels strictly below 0.1 EU/mg.
Selecting an optimal supplier for research-grade peptides requires evaluating critical operational and manufacturing standards. Inconsistent peptide purity, residual trifluoroacetate (TFA) salt contamination, and unverified endotoxin content introduce significant confounding variables into cellular models.
When sourcing Chonluten for academic or industrial laboratory use, procurement teams should prioritize suppliers that provide full lot transparency. PX1 Research adheres to strict supply chain standards:
1. Domestic USA Manufacturing: All peptide synthesis and lyophilization take place in United States facilities operated under current Good Manufacturing Practice (cGMP) guidelines. 2. Independent ISO 17025 Testing: COAs are generated by independent analytical testing facilities, detailing raw HPLC chromatograms and mass spectra. 3. Lot Traceability: Batch numbers are linked directly to raw material provenance, synthesis logs, and physical retention samples.
For institutions acquiring peptides in bulk volumes for multi-phase study protocols, explore options for bulk research peptides to secure unified lot reservation and dedicated quality documentation.
Chonluten is supplied as a sterile, lyophilized white powder to maximize chemical stability during transport and initial storage. Correct handling and reconstitution protocols are vital to preserving the peptide's structural integrity and preventing enzymatic degradation.
For reconstitution, laboratory personnel should allow the vial to equilibrate to room temperature before opening to minimize condensation. Reconstitution should be performed using Bacteriostatic Water (0.9% benzyl alcohol) or Sterile Lyophilization-grade Water, depending on the requirements of downstream culture assays. Gently swirl the vial to dissolve the cake; mechanical agitation or vigorous shaking must be avoided to prevent peptide aggregation.
Reconstituted solutions should be aliquoted into single-use polypropylene tubes to prevent repeated freeze-thaw cycles. Store working aliquots at 2°C to 8°C for short-term use (up to 7 days), or at -20°C to -80°C for extended experimental periods. Review our full guidance on short-chain peptide stability for comprehensive storage parameters.
PX1 Research operates as a dedicated American supplier of research chemicals and bioregulatory peptides, serving academic laboratories, biotechnology enterprises, and institutional research facilities. Our operational architecture is optimized to eliminate supply chain delays and maintain strict cold-chain compliance.
Orders placed before daily cutoffs ship same-day from our distribution centers located in California and Arizona. Every shipment includes lot-specific, downloadable Certificates of Analysis containing verified chromatographic and spectroscopic data.
By enforcing ISO 17025 accredited analytical verification and maintaining domestic GMP-compliant production facilities, PX1 Research provides laboratories with the purity, consistency, and analytical transparency necessary for advance research into antioxidant enzyme regulation and tissue biology.
What is the sequence and chemical formula of Chonluten?
Chonluten is a synthetic tripeptide with the amino acid sequence L-glutamyl-L-aspartyl-L-glycine (Glu-Asp-Gly). Its molecular formula is C11H17N3O8 with a theoretical molecular weight of approximately 319.27 g/mol.
How does Chonluten influence antioxidant enzyme regulation in vitro?
Preclinical in vitro studies suggest Chonluten modulates gene expression via Nrf2 pathways, leading to increased mRNA and protein expression of key antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx).
What purity levels are guaranteed for PX1 Research Chonluten?
PX1 Research provides Chonluten with guaranteed purity equal to or exceeding 98.0% as verified by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and mass spectrometry analysis.
What endotoxin limits apply to Chonluten supplied by PX1 Research?
Every lot of Chonluten undergoes Limulus Amebocyte Lysate (LAL) testing to ensure endotoxin levels remain strictly below 0.1 EU/mg, preventing cell culture contamination or unspecific immune activation.
How should lyophilized Chonluten be stored upon arrival?
Lyophilized Chonluten should be stored at -20°C or -80°C for long-term stability. Desiccated vials can be kept at 2°C to 8°C for short-term storage prior to reconstitution.
What solvents are recommended for reconstituting Chonluten in a research lab?
Chonluten is readily soluble in aqueous solutions. Recommended solvents include Sterile Water for Injection, Phosphate-Buffered Saline (PBS, pH 7.4), or Bacteriostatic Water depending on cellular assay constraints.
Where is PX1 Research Chonluten manufactured and shipped from?
PX1 Research manufactures its peptides in USA-based, GMP-compliant facilities. Orders are fulfilled directly from distribution facilities in California and Arizona with same-day shipping on business days.
How does Chonluten differ from Bronchogen in research applications?
While both are respiratory-focused bioregulatory peptides, Chonluten is a tripeptide (Glu-Asp-Gly) primarily evaluated for antioxidant enzyme regulation and gene modulation, whereas Bronchogen is a tetrapeptide (Ala-Glu-Asp-Leu) frequently studied for extracellular matrix remodeling and tissue repair.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.