Reviewing an authentic AOD-9604 test report requires verifying lot-specific RP-HPLC purity above 99%, mass spectrometry identity confirmation, and low endotoxin levels. PX1 Research supplies USA-manufactured AOD-9604 with independent third-party certificates of analysis (COA) for every batch, dispatched same-day M–F from California and Arizona facilities.
Reviewing an authentic AOD-9604 test report requires verifying lot-specific RP-HPLC purity above 99%, mass spectrometry identity confirmation, and low endotoxin levels. PX1 Research supplies USA-manufactured AOD-9604 with independent third-party certificates of analysis (COA) for every batch, dispatched same-day M–F from California and Arizona facilities.
A rigorous AOD-9604 test report provides verifiable documentation that a synthetic peptide batch meets precise chemical standards required for bench science and analytical testing. When inspecting laboratory documentation, principal investigators must evaluate three non-negotiable quantitative metrics: purity percentage via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), molecular identity via Mass Spectrometry (MS), and bio-burden safety via bacterial endotoxin testing.
A standard certificate of analysis (COA) should explicitly state the batch number, date of analysis, theoretical versus observed molecular weight, and chromatogram raw data. High-grade research material must exhibit a primary RP-HPLC peak area corresponding to greater than 99.0% purity, with secondary synthesis truncated sequences accounting for less than 1.0% of total integration.
At PX1 Research, every batch of AOD-9604 5mg undergoes exhaustive testing at accredited, independent ISO/IEC 17025 third-party laboratories within the United States. We make full raw analytical data available for direct lot matching prior to procurement, ensuring reproducible baseline conditions across cellular and animal research models.
AOD-9604 (Advanced Obesity Drug 9604) is a C-terminal peptide fragment derived from human growth hormone (hGH), specifically encompassing amino acids 177 through 191 with an added tyrosine residue at the N-terminus (Tyr-hGH 177-191). Its chemical structure consists of 16 amino acids forming a specific tertiary conformation stabilized by an intramolecular disulfide bridge between cysteine residues at positions 7 and 14.
In structural biology and preclinical research, AOD-9604 is investigated for its distinct mechanism of action relative to intact somatotropin. While native hGH binds to growth hormone receptors to stimulate IGF-1 release and systemic growth cascades, preclinical models demonstrate that the 177-191 fragment specifically interacts with lipolytic pathway pathways without triggering diabetogenic or anabolic signaling cascades.
Verifying the precise primary sequence and disulfide linkage is critical during analytical verification. Incorrectly folded synthetic analogs or missing disulfide bonds dramatically alter peptide stability and binding kinetics in cell culture assays. Consequently, a comprehensive AOD-9604 test report must confirm both sequence identity and structural integrity before entering laboratory workflows.
Evaluating chemical analytical reports requires understanding the primary testing methodologies utilized by specialized peptide testing facilities. A fully compliant COA combines chromatographic separation, mass determination, and microbiological testing to validate chemical integrity.
1. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): RP-HPLC separates chemical compounds based on hydrophobicity as they pass through a stationary hydrophobic column under high pressure. The resulting chromatogram displays a dominant absorbance peak (measured at 214 nm or 220 nm). The relative peak area calculates purity. Quality reference standards demand an RP-HPLC purity score exceeding 99.0%.
2. Electrospray Ionization Mass Spectrometry (ESI-MS) or MALDI-TOF: Mass spectrometry confirms the exact molecular weight of the synthesized peptide. Theoretical molecular mass for AOD-9604 (C78H123N23O23S2) is approximately 1815.1 Da. An accurate mass spectrum shows a sharp mass-to-charge (m/z) ratio peak directly corresponding to the expected monoisotopic or average mass, confirming that the synthesized chain matches the target sequence without missing amino acid residues.
3. Bacterial Endotoxin Testing (LAL Assay): Endotoxins are lipopolysaccharide (LPS) complexes originating from bacterial cell walls during synthesis or handling. High endotoxin content introduces inflammatory artifacts that alter cell culture signaling or animal physiology. Premium laboratory reagents must yield an endotoxin concentration under 0.05 EU/mg using kinetic chromogenic Limulus Amebocyte Lysate (LAL) testing.
Researchers evaluating prospective suppliers should apply strict criteria when reviewing documentation. The list below outlines the standard analytical thresholds required for bench-grade research peptides.
Purity Verification: Must show ≥99.0% primary peak area integration on RP-HPLC chromatograms. Broad or overlapping peaks indicate batch degradation or incomplete peptide synthesis.
Molecular Weight Confirmation: MS spectrum must match theoretical mass (1815.1 Da) within a ±1.0 Da tolerance window, verifying chemical identity without truncations.
Endotoxin Quantification: Assayed levels must fall below 0.05 EU/mg. Documentation omitting endotoxin metrics presents significant risk for sensitive in vitro and in vivo assays.
Lot Traceability: COAs must detail distinct lot numbers that directly match the QR code or printed physical label affixed to the sealed glass vial.
Independent Testing Accreditation: Third-party testing must be conducted by an independent US-based analytical laboratory, independent of the primary manufacturing facility.
Complete Raw Chromatograms: Test reports must display the full baseline-resolved chromatographic scan (x-axis runtime, y-axis absorbance) rather than simplified summary text.
Selecting a research supplier requires examining operational transparency, analytical rigor, and logistics speed. PX1 Research adheres to stringent internal quality controls to support precise scientific reproduction.
Vendor Verification Metrics:
- Manufacturing Origin: USA-synthesized and processed under cGMP-compliant automated solid-phase peptide synthesis (SPPS) protocols, versus unverified overseas bulk import.
- Batch-Specific Testing: Every single production run undergoes fresh independent RP-HPLC and ESI-MS testing, compared to vendors using static or outdated COAs across multiple lots.
- Endotoxin Safety: Explicit LAL assay results provided for all lots, whereas standard commercial suppliers frequently omit bacterial toxin testing entirely.
- Shipping Speed: Same-day dispatch for orders placed before 3:00 PM EST (Monday–Friday) from fulfillment centers in California and Arizona, avoiding temperature degradation in long transit windows.
- Analytical Support: Direct access to raw data sets and technical support specialists ready to verify lot numbers against archival laboratory records.
To review our complete catalog of certified compounds alongside their corresponding laboratory documentation, explore our all peptides research library or review our peptide research documentation.
In addition to AOD-9604, investigators frequently compare structural and functional data against other C-terminal fragments and growth hormone secretagogues in related preclinical studies. You can browse these related items across our growth hormone secretagogue category.
1. Fragment 176-191: A closely related unsubstituted peptide fragment derived from human growth hormone (hGH 176-191). While AOD-9604 contains an added N-terminal tyrosine residue to enhance stability, unmodified Fragment 176-191 serves as a classical baseline control in comparative metabolic and lipolytic signaling research.
2. CJC-1295 DAC: A synthetic tetrasubstituted 29-amino acid peptide analog of Growth Hormone-Releasing Hormone (GHRH). It incorporates a Drug Affinity Complex (DAC) moiety to extend plasma half-life, making it a primary research candidate alongside fragments when examining sustained pituitary somatotrope activation.
3. Tesamorelin 10mg: A stabilized trans-3-hexenoyl derivative of GHRH approved for studying ectopic visceral adipose reduction and endogenous growth hormone release dynamics in structural biology setups.
Investigators interested in high-volume research applications can explore bulk procurement pathways directly through our wholesale peptide program.
The prevalence of falsified or misleading analytical documentation poses a challenge for laboratory procurement officers. Identifying fraudulent COA reports requires scrutinizing analytical files for common anomalies.
A primary red flag is a blurred, cropped, or low-resolution HPLC chromatogram where axis labels, integration tables, or run times are illegible. Vendors may crop out secondary degradation peaks or adjust baseline noise to artificially inflate reported purity scores.
Another critical red flag is the absence of a unique, cross-verifiable lot number. If a COA lacks a distinct batch code that directly corresponds to the container label in your shipment, the document is generic and uninformative. Furthermore, verify that the testing facility is an established, independent testing lab rather than an internal, non-accredited marketing division.
Lastly, beware of test reports that show an identical mass spectrometry curve across entirely different product listings. Each distinct peptide peptide sequence possesses a unique molecular signature; identical spectra across multiple compounds indicate templated graphics rather than authentic laboratory analysis.
AOD-9604 is delivered as a lyophilized (freeze-dried) cake or powder inside a sealed, sterile glass vial. To preserve primary peptide sequence stability and prevent premature enzymatic or hydrolytic cleavage, scientific personnel should follow standard aseptic reconstitution workflows.
Upon arrival, un-reconstituted lyophilized vials should be stored in a controlled cold environment: short-term storage (under 30 days) at 2°C to 8°C, or long-term storage (up to 24 months) in a sub-zero freezer at -20°C or -80°C. Protect lyophilized cakes from direct UV light exposure.
Reconstitution Protocol for Analytical Application:
- Allow the vial to equalize to room temperature prior to reconstitution to prevent moisture condensation within the glass container.
- Swab the rubber septum with 70% isopropyl alcohol using a sterile wipe inside a laminar flow hood.
- Using a sterile laboratory syringe, slowly introduce an appropriate volume of Bacteriostatic Water (0.9% benzyl alcohol) or sterile laboratory-grade water along the inner glass wall of the vial.
- Allow the solvent to gently submerge the lyophilized cake. Gently swirl the vial in a slow circular motion. Never shake or vortex the vial vigorously, as high shear mechanical forces can disrupt sensitive secondary structural folds and disulfide bonds.
- Once fully reconstituted into a clear, colorless liquid, store the solution refrigerated at 2°C to 8°C and use within 28 days.
PX1 Research is committed to supplying verified, high-purity compounds to academic institutions, biotechnology organizations, and independent scientific researchers across North America. When procuring AOD-9604 vials, your order is backed by complete batch-level analytical transparency.
What Ships with Every Order:
- Lyophilized AOD-9604 in sealed 5mg vacuum glass vials featuring tamper-evident packaging.
- Direct digital access to lot-matched RP-HPLC and ESI-MS test reports verified by accredited independent US testing facilities.
- Climate-controlled protective packaging designed to maintain thermal integrity during domestic transit.
- Rapid same-day dispatch for all orders placed before 3:00 PM EST, Monday through Friday, operating directly out of our California and Arizona logistics hubs.
- Dedicated responsive customer support for technical inquiries, batch matching, and bulk fulfillment requests.
To review current inventory levels, request specific COA archives, or finalize your order, visit our dedicated AOD-9604 product page or contact our client support team today.
What is an AOD-9604 test report?
An AOD-9604 test report is a formal Certificate of Analysis (COA) generated by an independent analytical testing laboratory. It details the synthetic peptide's chemical purity (via RP-HPLC), exact molecular mass (via ESI-MS mass spectrometry), and microbiological endotoxin safety levels (via LAL testing) for a specific production batch.
How do I check the HPLC purity on an AOD-9604 COA?
Review the RP-HPLC chromatogram table included on the test report. Locate the primary chemical peak absorbance runtime (typically measured at 214 nm or 220 nm) and check the integrated peak area percentage. Premium research-grade AOD-9604 must demonstrate a primary peak integration of 99.0% or higher.
What is the expected molecular weight on an AOD-9604 test report?
The theoretical molecular weight of AOD-9604 (C78H123N23O23S2) is approximately 1815.1 Daltons (g/mol). An authentic mass spectrometry scan within the test report should display a clear mass-to-charge (m/z) peak corresponding directly to this value within a ±1.0 Da tolerance window.
Why is endotoxin testing critical for AOD-9604 research?
Endotoxin testing ensures that residual bacterial lipopolysaccharides from peptide processing are below hazardous thresholds (<0.05 EU/mg). High endotoxin concentrations introduce severe cell culture inflammation artifacts or physiological shock in preclinical animal models, skewing research data.
Does PX1 Research provide a COA for every AOD-9604 lot?
Yes. PX1 Research tests every batch of AOD-9604 through independent ISO/IEC accredited US third-party laboratories. Every vial features a lot number that matches published, downloadable RP-HPLC, mass spec, and endotoxin analytical certificates.
How should lyophilized AOD-9604 be stored upon delivery?
Lyophilized AOD-9604 should be stored in a dry, dark place refrigerated between 2°C and 8°C for immediate use (under 30 days), or frozen at -20°C to -80°C for long-term storage up to 24 months. Reconstituted solution must remain refrigerated at 2°C to 8°C.
What liquid is used to reconstitute AOD-9604 for laboratory research?
AOD-9604 is typically reconstituted using sterile Bacteriostatic Water (0.9% benzyl alcohol) or sterile water for injection, introduced gently along the inner vial wall to avoid mechanical shearing of the secondary peptide structure.
What is the structural difference between AOD-9604 and Fragment 176-191?
AOD-9604 is a modified derivative of hGH Fragment 176-191 that includes an added tyrosine residue at the N-terminus. This structural addition enhances chemical stability during synthesis and handling while preserving target C-terminal peptide signaling properties.
How fast does PX1 Research ship AOD-9604 orders?
PX1 Research dispatches orders same-day Monday through Friday for purchases placed prior to 3:00 PM EST. Packages ship via fast tracked domestic delivery directly from fulfillment centers located in California and Arizona.
Is AOD-9604 permitted for human consumption?
No. AOD-9604 supplied by PX1 Research is strictly designated for laboratory research use only (RUO), including in vitro cellular assays and preclinical animal research. It is strictly prohibited for human or veterinary medical use.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.