Bronchogen is a short peptide bioregulator actively investigated in pulmonary cell culture and animal models of bronchial tissue integrity. This resource provides clear pricing criteria, structural data, and analytical parameters for institutional researchers evaluating Bronchogen in bronchoalveolar lavage (BAL) protocols.
Bronchogen is a short peptide bioregulator actively investigated in pulmonary cell culture and animal models of bronchial tissue integrity. This resource provides clear pricing criteria, structural data, and analytical parameters for institutional researchers evaluating Bronchogen in bronchoalveolar lavage (BAL) protocols.
The price to buy research-grade Bronchogen for bronchoalveolar lavage (BAL) assays typically ranges from $40 to $90 per vial, depending on unit mass, analytical purity verification, and volume tier. High-purity Bronchogen (>98% RP-HPLC) manufactured in USA ISO 17025 facilities with lot-specific mass spectrometry and endotoxin testing ensures reproducible quantitative data in respiratory cell line and BAL fluid protocols.
When planning experimental runs involving pulmonary lavage markers, institutional buyers must evaluate cost relative to analytical integrity. Unverified or low-purity peptides risk introducing foreign endotoxins or chemical artifacts into sensitive BAL fluid analysis, compromising cytokine quantification, cellular differential counts, and transcriptomic profiling. PX1 Research supplies standardized Bronchogen research vials engineered specifically for rigorous in vitro and preclinical research applications.
Bronchogen is a synthetic peptide bioregulator composed of four amino acid residues (Ala-Asp-Glu-Leu, or ADEL). Belonging to the class of short peptide regulators initially conceptualized in biogerontology, its compact molecular structure facilitates cellular uptake and nuclear interaction in target bronchopulmonary tissue preparations.
In analytical assays, Bronchogen presents a distinct monoisotopic mass and hydrophobicity profile accessible via reverse-phase high-performance liquid chromatography (RP-HPLC) and electrospray ionization mass spectrometry (ESI-MS). Because of its small molecular weight, verifying peptide sequence fidelity and salt content (typically trifluoroacetate or acetate counterions) is vital prior to introducing the peptide into bronchial tissue culture or ex vivo pulmonary perfusion systems.
Researchers exploring gene expression patterns in respiratory tissues often reference our comprehensive guide on Khavinson peptide bioregulators, which details the conformational interactions between short peptides and genomic DNA histone complexes.
Preclinical studies indicate that Bronchogen interacts with bronchial epithelial cells, fibroblasts, and local alveolar macrophages. In vitro model systems evaluating respiratory tract homeostasis demonstrate that short synthetic peptides can modulate protein synthesis and gene expression involved in mucociliary clearance and epithelial barrier integrity.
In animal models subjected to environmental stressors or oxidative insult, administration of Bronchogen has been associated with alterations in cellular repair pathways. Preclinical findings suggest that the peptide may influence the transcription of genes encoding structural proteins, such as cytokeratins, as well as tight-junction proteins like occludins and claudins in bronchial epithelial monolayers.
To review additional technical literature on respiratory cell signaling models, explore the PX1 Research Library, where empirical studies on peptide-mediated gene regulation are compiled for institutional cross-reference.
Bronchoalveolar lavage (BAL) is a fundamental preclinical technique used to assess the inflammatory, cellular, and biochemical state of the respiratory tract. In laboratory rodents or ex vivo lung models, micro-volumes of sterile saline are instilled into the bronchial tree and recovered for downstream analysis.
When evaluating Bronchogen within BAL experimental designs, researchers isolate the supernatant and cellular pellet to quantify specific biomarkers. Primary parameters measured in post-treatment BAL fluid include total and differential cell counts (macrophages, neutrophils, lymphocytes), total protein concentration (indicating vascular permeability), and expression levels of pro-inflammatory cytokines such as TNF-alpha, IL-1beta, and IL-6.
Preclinical models evaluating peptide activity in BAL assays require zero baseline endotoxin interference. Contaminants in low-tier peptide reagents can trigger non-specific toll-like receptor 4 (TLR4) activation in alveolar macrophages, skewing cytokine readings and invalidating BAL data. Ensuring low endotoxin limits (<0.01 EU/mg) is therefore an absolute requirement for valid research outcomes.
Within the landscape of short peptide regulators targeting cardiopulmonary and immune systems, Bronchogen is frequently compared against other short-chain peptides such as Chonluten and Vilon. While Bronchogen specifically targets bronchial tissue gene expression profiles, Chonluten exhibits overlapping pulmonary specificity with distinct amino acid sequencing.
In contrast, Vilon (Lys-Glu) functions primarily as a systemic immunomodulatory peptide evaluated in thymic and splenic leukocyte activation assays rather than direct respiratory epithelial cell models. Another peptide frequently studied alongside pulmonary bioregulators is Thymogen, which focuses on T-cell differentiation pathways.
The following matrix summarizes key structural and targeted assay parameters across these laboratory research compounds:
- **Bronchogen (Ala-Asp-Glu-Leu):** Selective for bronchial epithelial tissue models, mucociliary gene expression, and BAL cytokine profiling. - **Chonluten (Glu-Asp-Gly):** Evaluated for respiratory repair mechanism assays and pulmonary oxidative stress models. - **Vilon (Lys-Glu):** Applied in general immune cell activation, chromatin condensation assays, and thymocyte proliferation studies.
When purchasing Bronchogen for delicate BAL assays, chemical purity cannot be assumed solely based on supplier catalog descriptions. PX1 Research subjects every synthesis lot to rigorous multi-step analytical validation conducted in an independent, ISO 17025-accredited laboratory.
Purity is verified using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), ensuring a minimum threshold of 98.0% target peptide peak area. Electrospray Ionization Mass Spectrometry (ESI-MS) confirms exact monoisotopic mass and rules out deletion sequences or incomplete coupling products.
Furthermore, because BAL assays utilize live cellular fractions and sensitive immunoassay kits, every lot undergoes chromogenic Limulus Amebocyte Lysate (LAL) testing to certify endotoxin levels below strictly controlled limits. Every order shipped from our California and Arizona fulfillment centers includes a batch-specific Certificate of Analysis (COA) detailing these quantitative metrics.
Bronchogen is supplied as a lyophilized (freeze-dried) sterile powder to ensure maximum chemical stability during transit and storage. Reconstitution protocols must adhere to strict aseptic techniques within a certified laminar flow biosafety cabinet to prevent biological contamination.
For in vitro tissue culture assays and BAL fluid spike experiments, the lyophilized powder should be reconstituted using Bacteriostatic Water (0.9% benzyl alcohol) or sterile endotoxin-free phosphate-buffered saline (PBS, pH 7.4). Avoid high-shear vortexing; gentle agitation or mild inversion promotes complete dissolution without mechanical peptide shear.
For long-term preservation, lyophilized Bronchogen should be stored desiccated at -20°C or -80°C. Following reconstitution, liquid aliquots should be stored at -20°C to prevent freeze-thaw cycles, which can degrade short peptide chains over extended periods. Detailed reconstitution math and vehicle compatibility guides are available through the main all research peptides library.
To accommodate various project scales—from preliminary pilot assays to multi-arm animal model runs—PX1 Research provides tiered pricing structures for verified research facilities. Standard unit pricing for individual 20mg research vials starts at competitive baseline rates, with significant volume discounting applied for institutional purchases.
Academic laboratories, contract research organizations (CROs), and industrial R&D departments requiring multi-gram quantities or custom lot sizes can establish institutional accounts via our wholesale procurement portal. Institutional accounts benefit from dedicated account management, reserved single-lot batch allocation to minimize inter-assay variability, and expedited same-day dispatch from our US warehouse hubs.
What is Bronchogen used for in laboratory research?
Bronchogen (Ala-Asp-Glu-Leu) is a research compound studied in preclinical models to evaluate gene expression, cellular repair mechanisms, and barrier function in bronchial epithelial cells and bronchoalveolar lavage (BAL) models.
What factors influence the price when I buy Bronchogen?
Pricing depends on vial quantity (mg mass), verified purity levels (>98% via RP-HPLC), third-party endotoxin testing, and unit order volume. Institutional volume tiers lower the per-vial cost significantly.
Why is endotoxin testing critical for Bronchogen used in BAL fluid assays?
Endotoxins introduce non-specific immune activation via TLR4 signaling in alveolar macrophages. Low endotoxin limits (<0.01 EU/mg) ensure that BAL cellular counts and cytokine profiles reflect pure experimental variables rather than contamination artifacts.
How is Bronchogen verified for chemical identity and purity?
PX1 Research verifies every lot via RP-HPLC for chemical purity and mass spectrometry (ESI-MS) for sequence accuracy. Independent ISO 17025 lab reports are provided on every product COA.
What solvent is recommended for reconstituting Bronchogen for in vitro work?
Sterile endotoxin-free PBS (pH 7.4) or sterile research-grade water is recommended for acute cell culture or BAL protocols. For extended storage of reconstituted solutions, bacteriostatic water may be used under sterile conditions.
Is Bronchogen approved for human consumption or therapeutic use?
No. Bronchogen is strictly designated for laboratory in vitro and preclinical research use only. It is not for human or animal therapeutic, diagnostic, or clinical application.
How does Bronchogen compare to Chonluten in pulmonary research?
Both are short peptide bioregulators evaluated in respiratory models; however, Bronchogen consists of the tetrapeptide Ala-Asp-Glu-Leu, while Chonluten is a tripeptide (Glu-Asp-Gly). They target overlapping yet distinct gene regulatory pathways in bronchial cells.
What are the storage requirements for lyophilized Bronchogen?
Lyophilized powder should be stored at -20°C for long-term stability. Upon reconstitution, aliquot the solution and freeze at -20°C or -80°C, avoiding repeated freeze-thaw cycles.
Where does PX1 Research manufacture and ship Bronchogen from?
PX1 Research compounds are manufactured under strict GMP-compliant standards in the USA and shipped same-day (Monday–Friday) from facility hubs in California and Arizona.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.