Cardiogen is a synthetic tetrapeptide (Ala-Glu-Asp-Arg) investigated for its tissue-specific regulatory effects on cardiovascular cell models and cardiac fibroblast proliferation. PX1 Research supplies USA-manufactured, high-purity Cardiogen designed strictly for in vitro assays, preclinical research, and analytical investigations.
Cardiogen is a synthetic tetrapeptide (Ala-Glu-Asp-Arg) investigated for its tissue-specific regulatory effects on cardiovascular cell models and cardiac fibroblast proliferation. PX1 Research supplies USA-manufactured, high-purity Cardiogen designed strictly for in vitro assays, preclinical research, and analytical investigations.
To buy Cardiogen for laboratory research use only, institutional investigators and research personnel require verified reference materials meeting rigorous chemical standards. High-purity Cardiogen is a synthetic tetrapeptide designed specifically for in vitro experimentation, cell culture assays, and preclinical physiological models.
When purchasing research compounds, establishing supplier legitimacy through analytical documentation is critical. PX1 Research provides fully transparent, lot-specific Certificates of Analysis (COAs) utilizing reverse-phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry (MS) to confirm sequence identity, structural purity, and absence of endotoxins.
Cardiogen belongs to the short peptide bioregulator family originally characterized in peptide biochemistry literature. Composed of four amino acid residues—L-alanyl-L-glutamyl-L-aspartyl-L-arginine (sequence: Ala-Glu-Asp-Arg or AEDR)—it possesses a molecular formula of C18H31N7O9 and a molecular mass of approximately 489.48 g/mol.
Due to its minimal molecular weight, Cardiogen displays distinct pharmacokinetic and biochemical handling characteristics compared to larger proteins. The specific ionic charges provided by the acidic aspartic and glutamic acid residues alongside the basic arginine terminal enable specific binding interactions in cell culture media models. Qualified laboratories can inspect raw sequence integrity across our entire catalog of all research peptides to ensure experimental precision.
In vitro studies demonstrate that short bioregulatory peptides such as Cardiogen interact directly with chromatin structures and specific promoter regions of DNA. Preclinical literature suggests that the AEDR sequence can penetrate nuclear membranes in cultured cardiovascular cells, influencing the expression profile of regulatory proteins.
Primary cardiac fibroblast models exposed to Cardiogen show altered proliferation rates and modulated collagen synthesis signaling pathways. Researchers examining extracellular matrix dynamics utilize this peptide to evaluate localized tissue remodeling pathways without the confounding influence of systemic neurohormonal signals.
Preclinical investigations utilizing rodent myocardial infarction models and isolated cardiomyocyte cultures have evaluated Cardiogen for its potential role in cellular survival mechanisms. In vitro assays indicate that exposure to Cardiogen may alter p53 expression and downregulate pro-apoptotic signaling pathways under oxidative stress conditions.
Furthermore, animal models investigating ischemic conditions suggest that short peptide administration may support localized microvascular response studies. Researchers exploring structural tissue responses can review comprehensive mechanisms in the PX1 Research library, where preclinical data across multiple cellular pathways are detailed.
Cardiogen is often evaluated alongside other short-chain synthetic bioregulators developed for targeted tissue research. While Cardiogen (AEDR) focuses primarily on cardiomyocyte and cardiac fibroblast gene pathways, Vesugen (Lys-Glu-Asp) is a tripeptide primarily investigated in vascular endothelial cell and smooth muscle tissue models. Both compounds share a short peptide backbone capable of nuclear translocation in vitro, yet target distinct cell surface and intracellular cascades.
Additionally, broader systemic short peptides like Epitalon (Ala-Glu-Asp-Gly) and Livagen (Lys-Glu-Asp-Pro) are routinely incorporated into comparative studies analyzing telomerase activity, chromatin condensation, and cellular aging kinetics. For comparative tissue-repair models outside the cardiovascular system, investigators frequently contrast these bioregulators with regenerative signaling compounds like BPC-157.
Maintaining experimental reproducibility requires stringent purity verification for every lot of research peptides. Substandard or crude synthetic peptides often contain truncated sequences, deletion sequences, or residual cleavage reagents that interfere with delicate cell culture signaling assays.
Every batch of Cardiogen 5mg supplied by PX1 Research undergoes rigorous testing in an ISO 17025 accredited laboratory. Purity is validated via RP-HPLC, ensuring a threshold exceeding 98.0%, while Electrospray Ionization Mass Spectrometry (ESI-MS) confirms precise monoisotopic mass. Lot-specific COAs are published directly to allow complete auditability prior to experimental integration.
Bacterial endotoxins (lipopolysaccharides) present a major variable in cell culture and animal tissue models, as minute concentrations can trigger unspecific inflammatory cascades, confounding gene expression data. Standard biochemical synthesis must be coupled with downstream purification to eliminate these pyrogenic contaminants.
PX1 Research enforces strict endotoxin screening protocols utilizing Limulus Amebocyte Lysate (LAL) assays on all finished lyophilized compounds. Products are verified to contain less than 0.01 EU/mg, meeting criteria suitable for sensitive cell viability, flow cytometry, and transcriptomic analytical procedures.
Cardiogen is supplied as a sterile, lyophilized white powder to maximize shelf life and chemical stability during transport. Prior to utilizing the compound in experimental assays, proper reconstitution under sterile laboratory conditions is necessary.
Laboratories should reconstitute lyophilized Cardiogen using sterile, laboratory-grade Bacteriostatic Water or sterile 0.9% Sodium Chloride injection USP. Gently swirl the vial to dissolve the cake completely; vigorous mechanical vortexing should be avoided to prevent peptide shear or aggregation. All reconstitutions must be performed within an ISO Class 5 laminar flow hood to maintain sterility.
Lyophilized Cardiogen should be stored at -20°C upon receipt for long-term storage, protected from light and moisture. Under these conditions, the dry peptide cake remains stable for up to 24 months without significant degradation.
Once reconstituted into aqueous solution, aliquots should be used immediately or stored at 4°C for short-term use (up to 7 days). For extended analytical series, reconstitutions should be divided into single-use sub-aliquots and frozen at -80°C to avoid repeated freeze-thaw cycles, which accelerate physical degradation of the peptide sequence.
PX1 Research manufactures its catalog within GMP-compliant facilities located in the United States. Fast logistics are guaranteed, with orders shipping same-day Monday through Friday from fulfillment centers located in California and Arizona.
Principal investigators, university departments, and commercial biotechnology institutions requiring high-volume orders or recurring delivery schedules can apply for a dedicated wholesale lab account. This ensures bulk batch consistency, dedicated account support, and direct access to customized analytical reporting.
What is Cardiogen and how is it defined in research settings?
Cardiogen is a synthetic tetrapeptide with the amino acid sequence Ala-Glu-Asp-Arg (AEDR). It is categorized as a short bioregulatory peptide and is studied exclusively in laboratory settings for its interactions with cardiovascular cell culture models and gene expression pathways.
What analytical purity guarantees are provided when buying Cardiogen from PX1 Research?
PX1 Research provides a lot-specific Certificate of Analysis (COA) for every batch of Cardiogen. Purity is independently verified at over 98.0% using Reverse-Phase HPLC, with molecular weight verified via Mass Spectrometry and endotoxins measured below 0.01 EU/mg.
Is Cardiogen approved for human consumption or therapeutic use?
No. Cardiogen is strictly sold as a research compound intended for in vitro laboratory research and preclinical testing only. It is not approved by the FDA for human or animal consumption, medical therapy, or clinical application.
How should lyophilized Cardiogen be stored upon delivery?
Lyophilized Cardiogen should be kept in a desiccated freezer environment at -20°C or -80°C for long-term stability. Upon receipt, vials should be stored away from direct light.
What is the recommended reconstitution procedure for laboratory assays?
Reconstitute the peptide using sterile bacteriostatic water or sterile saline under a laminar flow hood. Gently rotate the vial until the lyophilized cake is fully dissolved. Avoid excessive shaking or vortexing.
How does Cardiogen differ from the vascular bioregulator Vesugen?
While Cardiogen (AEDR) is primarily studied in cardiomyocyte and cardiac fibroblast cellular models, Vesugen (KED) is a tripeptide primarily investigated for its effects on vascular endothelial tissue and smooth muscle signaling.
Where are PX1 Research compounds manufactured and shipped from?
All PX1 Research compounds are manufactured in USA-based, GMP-compliant facilities and shipped directly from fulfillment centers located in California and Arizona.
Can academic labs order Cardiogen in bulk or wholesale quantities?
Yes. Institutional buyers and academic researchers can request a wholesale lab account through PX1 Research to access bulk pricing, lot reservation, and batch-matched analytical verification.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.