Buy CJC-1295 Without DAC (Mod GRF 1-29) for Preclinical Research

Chemical Profile & Structure: CJC-1295 Without DAC (Mod GRF 1-29)

CJC-1295 without DAC, chemically designated as Modified GRF 1-29 (Mod GRF 1-29), is a synthetic peptide analog of endogenous growth hormone-releasing hormone (GHRH). Natural GHRH consists of 44 amino acids, but bioactivity resides entirely within the N-terminal 29-amino-acid chain known as GRF 1-29 (sermorelin). To overcome the rapid enzymatic degradation of native GRF 1-29 by dipeptidyl peptidase-IV (DPP-IV), researchers engineered four specific amino acid substitutions at positions 2, 8, 15, and 27.

The resulting tetrasubstituted peptide sequence—Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2—exhibits significantly enhanced metabolic stability against plasma proteases. When investigators choose to buy CJC-1295 without DAC, they obtain a compound that mimics natural physiological GHRH release patterns without the covalent albumin-binding complex attached to the Drug Affinity Complex (DAC) variant.

In physiological and analytical settings, this molecular configuration allows researchers to observe acute, concentration-dependent receptor activation. Understanding these structural modifications is essential when evaluating peptide interactions within our broader catalog of research peptides.

Pharmacokinetics and Receptor Signaling Mechanism

CJC-1295 without DAC functions as a selective agonist at the growth hormone-releasing hormone receptor (GHRH-R), a G-protein-coupled receptor located on pituitary somatotropes. Upon receptor binding, Mod GRF 1-29 stimulates the Gαs subunit, activating adenylate cyclase and increasing intracellular cyclic adenosine monophosphate (cAMP). This signaling cascade triggers protein kinase A (PKA) activation, opening voltage-gated calcium channels and stimulating the exocytosis of stored growth hormone granules.

Unlike its long-acting counterpart containing the Drug Affinity Complex, CJC-1295 without DAC demonstrates an in vivo plasma half-life of approximately 30 minutes in rodent and mammalian models. This brief half-life preserves natural pulsatile GH release without inducing continuous, non-physiological receptor saturation. Preclinical data indicate that this pulsatile signaling promotes hepatic production of insulin-like growth factor 1 (IGF-1) while preserving endogenous receptor sensitivity.

Investigators interested in mapping downstream somatotrope signaling or cellular transcription dynamics can consult experimental literature within the PX1 Research library, where signaling profiles of various growth hormone secretagogues are documented.

Preclinical Applications: Tissue Repair and IGF-1 Upregulation

In vitro and animal models show that CJC-1295 without DAC plays a pivotal role in studying tissue regeneration, cellular proliferation, and extracellular matrix remodeling. By elevating transient GH pulses, the peptide accelerates downstream IGF-1 gene expression across muscle satellite cells, chondrocytes, and osteoblasts. Preclinical studies suggest that this cascade supports nitrogen retention and collagen synthesis in damaged connective tissue.

Research in rodent models demonstrates that transient GH pulses induced by Mod GRF 1-29 enhance satellite cell activation following mechanical microtrauma. Furthermore, cellular assays demonstrate upregulation of skeletal muscle protein synthesis via the Akt/mTOR signaling pathway. These properties make the peptide a core tool for investigating acute metabolic recovery, wound healing, and musculoskeletal repair.

In experimental models examining systemic tissue synthesis, investigators often evaluate CJC-1295 without DAC alongside tissue-protective compounds like BPC-157 or extracellular matrix regulators to observe potential synergism in cellular migration and extracellular matrix deposition.

Comparative Analysis: CJC-1295 Without DAC vs. Related Secretagogues

When evaluating GHRH analogs and secretagogues, laboratory researchers must distinguish between short-acting and long-acting configurations. The core distinction between CJC-1295 without DAC and CJC-1295 with DAC lies in the presence of the maleimidopropionic acid linker. To learn more about how these kinetic profiles alter pituitary receptor desensitization, review our technical breakdown on CJC-1295 with DAC vs without DAC.

While CJC-1295 without DAC activates the GHRH pathway directly, growth hormone secretagogue receptor (GHSR-1a) agonists act via a distinct, complementary mechanism. For instance, pairing a GHRH agonist with a selective ghrelin mimetic such as Ipamorelin or GHRP-2 produces a synergistic elevation in GH release in vitro. Alternatively, native Sermorelin represents the unmodified 29-amino-acid chain, which lacks the four stabilizing amino acid substitutions present in Mod GRF 1-29.

The table below outlines key biochemical differences across these preclinical secretagogues:

Sourcing Criteria: Evaluating CJC-1295 Without DAC for Sale

When sourcing reagents for sensitive assay protocols, verifying purity and manufacturer standards is essential. Prior to selecting CJC 1295 without DAC for sale, procurement officers and principal investigators should verify that the product meets stringent analytical benchmarks to eliminate experimental variability.

PX1 Research enforces rigorous quality assurance metrics across every synthesis lot, including:

• Purity Verification: High-Performance Liquid Chromatography (RP-HPLC) ensuring >99% chemical purity. • Identity Confirmation: Mass Spectrometry (ESI-MS) confirming exact molecular weight (3367.97 Da). • Endotoxin Testing: Kinetic Chromogenic LAL assay ensuring endotoxin levels below 0.01 EU/mg. • Domestic Synthesis: USA-manufactured under ISO 17025 and GMP-compliant conditions. • Lot Traceability: Certificate of Analysis (COA) provided with every vial.

Low-grade or unverified peptides often contain truncated sequences, trifluoroacetate (TFA) salt residues, or bacterial endotoxins that can contaminate cell cultures, induce cytotoxicity, or distort receptor binding kinetics. Sourcing from a verified laboratory partner ensures consistent baseline performance.

Laboratory Reconstitution Protocol and Solvent Compatibility

Reconstitution of CJC-1295 without DAC must be performed under aseptic conditions within a laminar flow hood to maintain sterile integrity. Lyophilized cake should be dissolved using sterile Bacteriostatic Water (0.9% benzyl alcohol) or Sterile Normal Saline (0.9% NaCl), depending on downstream application requirements.

To reconstitute the peptide correctly in a laboratory setting: 1. Sanitize the rubber stopper of the vial with a 70% isopropyl alcohol swab. 2. Direct the diluent stream gently against the glass wall of the vial rather than directly onto the lyophilized powder. 3. Swirl the vial gently with a slow rotational movement until complete dissolution occurs. Never shake or vortex the vial, as mechanical shear stress can cause peptide denaturation or aggregation. 4. Allow the solution to settle for 2–3 minutes until visual clarity is established.

For cell culture assays sensitive to benzyl alcohol, sterile water for injection (WFI) or phosphate-buffered saline (PBS, pH 7.4) should be utilized immediately prior to application.

Storage Dynamics and Stability Profile

Lyophilized CJC-1295 without DAC exhibits high thermal stability when stored under controlled environmental conditions. For long-term shelf storage prior to reconstitution, store lyophilized vials at -20°C to -80°C, protected from light and moisture. Under these conditions, the peptide remains stable for up to 24 months without significant degradation.

Once reconstituted in bacteriostatic water, the peptide solution should be stored at 2°C to 8°C (36°F to 46°F) and utilized within 30 days. Avoid repeated freeze-thaw cycles of reconstituted liquid, as phase transitions induce structural cleavage and peptide aggregation. If aliquoting is required for extended preclinical trial schedules, freeze reconstituted sub-aliquots once at -80°C and thaw immediately prior to use.

Ordering High-Purity Research Compounds from PX1 Research

PX1 Research is dedicated to providing accredited academic, corporate, and private research laboratories with high-purity reagents. When researchers choose to buy CJC 1295 without DAC through PX1, every order is backed by full analytical transparency, batch-specific COAs, and dedicated customer support.

Orders placed Monday through Friday before 3:00 PM EST ship same-day from our primary distribution hubs in California and Arizona, minimizing transit times and thermal exposure. Institutions needing bulk quantities or custom packaging can apply for a wholesale laboratory account to access volume pricing and dedicated account management.