Buy Epitalon Online
Qualified academic and institutional investigators seeking to buy Epitalon online can source analytical-grade material through PX1 Research. Each batch of synthesized Epitalon (Ala-Glu-Asp-Gly) undergoes rigorous mass spectrometry and HPLC purity verification to ensure exact sequence confirmation and lot-to-lot reliability for in vitro and animal models.
Quick answer
Qualified academic and institutional investigators seeking to buy Epitalon online can source analytical-grade material through PX1 Research. Each batch of synthesized Epitalon (Ala-Glu-Asp-Gly) undergoes rigorous mass spectrometry and HPLC purity verification to ensure exact sequence confirmation and lot-to-lot reliability for in vitro and animal models.
Key takeaways
- When purchasing synthetic peptides for biochemical analysis, selecting a domestic supplier with full supply-chain transparency is critical for reproducible scientific outcomes.
- Epitalon (also known as [Epithalon](/research-peptides/epithalon) or L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine) is a synthetic tetrapeptide with the amino acid sequence Ala-Glu-Asp-Gly and a molecular weight of approximately 390.35 g/mol.
- The primary biochemical target of interest in Epitalon research is the catalytic subunit of the telomerase enzyme complex (human telomerase reverse transcriptase, or hTERT).
- Telomeres—repetitive nucleoprotein structures located at the termini of linear chromosomes—progressively shorten during each cycle of DNA replication due to the end-replication problem.
Where to Buy Epitalon Online for Laboratory Research
When purchasing synthetic peptides for biochemical analysis, selecting a domestic supplier with full supply-chain transparency is critical for reproducible scientific outcomes. Researchers looking to buy Epitalon online require validated material free from counter-ion contamination, residual synthesis solvents, or bacterial endotoxins. PX1 Research supplies research-grade Epitalon manufactured within United States facilities adhering to stringent Good Manufacturing Practice (GMP) standards.
To safeguard experimental validity, every lot of Epitalon dispatched by PX1 Research is paired with an independent Certificate of Analysis (COA) issued by an ISO 17025-accredited laboratory. Orders ship directly from distribution centers in California and Arizona with same-day dispatch for purchases completed Monday through Friday before cut-off times, ensuring temperature-sensitive reagents arrive promptly to support ongoing preclinical workflows.
Overview of Epitalon: Chemical Structure and Classification
Epitalon (also known as Epithalon or L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine) is a synthetic tetrapeptide with the amino acid sequence Ala-Glu-Asp-Gly and a molecular weight of approximately 390.35 g/mol. Derived from early structural analyses of epithalamin—a crude polypeptide extract isolated from the bovine pineal gland—Epitalon belongs to a specialized class of short peptide sequences termed synthetic bioregulatory peptides.
Unlike long-chain polypeptide hormones or complex recombinant proteins, short tetrapeptides like Epitalon exhibit distinct physicochemical characteristics, including enhanced enzymatic stability in aqueous buffer solutions and specific nuclear interaction dynamics. In laboratory settings, Epitalon is utilized as a reference compound for investigating epigenetic gene regulation, histone modifications, and peptide-chromatin binding mechanisms without the steric hindrance often observed with larger protein structures.
Mechanism of Action: Telomerase Activation and Chromatin Remodeling
The primary biochemical target of interest in Epitalon research is the catalytic subunit of the telomerase enzyme complex (human telomerase reverse transcriptase, or hTERT). Preclinical studies suggest that Epitalon interacts directly with promoter regions of specific genes, facilitating chromatin unwinding and enhancing the transcriptional activity of the *TERT* gene. In vitro assays using somatic human cell lines demonstrate that exposure to Epitalon promotes upregulation of telomerase activity, resulting in the enzymatic elongation of telomeric repeat sequences (TTAGGG).
At the molecular level, researchers hypothesize that Epitalon induces local conformational changes in heterochromatin. In vitro binding assays show that the tetrapeptide sequence Ala-Glu-Asp-Gly preferentially binds to promoter regions rich in specific histone markers. This nuclear site-specific interaction allows investigators examining cellular signaling pathways to evaluate how low-molecular-weight bioregulators modulate gene expression patterns without altering the underlying genomic sequence.
Epitalon in Telomere Maintenance and Cellular Senescence Models
Telomeres—repetitive nucleoprotein structures located at the termini of linear chromosomes—progressively shorten during each cycle of DNA replication due to the end-replication problem. When telomere length drops below a critical threshold, cells initiate a DNA damage response leading to replicative senescence or apoptosis. Laboratory investigations using fetal human fibroblast culture models (such as WI-38 and IMR-90 cell lines) indicate that the addition of Epitalon to culture media leads to measurable telomere elongation and an extension of the Hayflick limit.
Furthermore, animal models evaluating rodent cellular aging show that treatment with Epitalon reduces the accumulation of senescent biomarkers, including beta-galactosidase activity and p16INK4a expression. Investigators monitoring oxidative stress markers in vitro have noted that Epitalon administration correlates with increased enzymatic activity of superoxide dismutase (SOD) and glutathione peroxidase, suggesting a dual mechanism involving direct genomic regulation and downstream protection against reactive oxygen species (ROS).
Circadian Rhythm Regulation and Melatonin Synthesis Research
In addition to its role in telomerase activation, Epitalon is extensively studied for its bioregulatory effects on the neuroendocrine system, specifically regarding pineal gland architecture and circadian rhythm maintenance. The pineal gland plays a central role in governing biological timing through the rhythmic secretion of melatonin. Preclinical research in rodent models demonstrates that Epitalon exposure restores baseline pineal ultrastructure and normalizes nocturnal melatonin secretion patterns altered by physiological aging or constant illumination protocols.
In vitro studies involving cultured pinealocytes indicate that Epitalon upregulates the expression of arylalkylamine N-acetyltransferase (AANAT)—the rate-limiting enzyme in the melatonin biosynthesis pathway. Consequently, researchers investigating biological chronometry utilize Epitalon to explore the biochemical crosstalk between pineal peptide bioregulators, suprachiasmatic nucleus (SCN) signaling, and systemic markers of circadian alignment.
Comparative Analysis: Epitalon vs. Other Bioregulatory Compounds
To properly contextualize research findings, investigators frequently evaluate Epitalon alongside other synthetic short-chain peptides targeting distinct physiological systems. While Epitalon specifically targets pineal function, telomere maintenance, and hTERT transcription, related bioregulatory compounds display unique tissue tropisms and receptor binding profiles.
For example, Thymalin is a thymus-derived peptide complex primary studied for its immunomodulatory effects and T-lymphocyte differentiation pathways. Similarly, Pinealon is a tripeptide (Glu-Asp-Arg) evaluated primarily in central nervous system models for neuroprotective and synaptic plasticity research. For studies focusing on senolytic mechanisms rather than telomerase activation, investigators often compare Epitalon with FOXO4-DRI, a targeted peptide designed to induce apoptosis selectively in senescent cells by disrupting p53-FOXO4 binding. Understanding these functional differences enables laboratories to select the precise peptide bioregulator required for their target experimental assays.
Quality Verification: HPLC, Mass Spectrometry, and Endotoxin Standards
Assaying short peptides requires robust analytical validation to confirm sequence fidelity and eliminate synthesis by-products. PX1 Research subjects every batch of Epitalon to rigorous multi-step testing. Purity is established using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), ensuring that target peptide content consistently exceeds 99.0% relative peak area. Mass spectrometry (ESI-MS or MALDI-TOF) is conducted concurrently to verify the exact molecular mass (390.35 Da) and eliminate the presence of deletion sequences or truncated peptide fragments.
Because Epitalon is frequently introduced to delicate primary cell cultures and sensitive animal models, controlling bacterial endotoxin levels is essential. Lipopolysaccharides (LPS) can provoke non-specific inflammatory responses in vitro, confounding experimental data. PX1 Research enforces strict limits on endotoxin content (<0.01 EU/mg verified via Chromogenic LAL assay). Researchers reviewing batch documentation through our portal can inspect raw chromatograms, mass spectra, and endotoxin assay reports prior to purchase.
Laboratory Reconstitution and Handling Protocols
Epitalon is supplied as a lyophilized (freeze-dried) sterile powder in sealed glass vials under vacuum. Lyophilization preserves the structural integrity of the peptide bonds during transport and short-term storage. Upon receipt, unprocessed lyophilized vials should be stored in a freezer at -20°C or -80°C protected from light exposure.
For laboratory reconstitution, researchers should allow the glass vial to equilibrate to room temperature before opening to prevent condensation from introducing moisture. Epitalon demonstrates high solubility in aqueous solutions, including sterile bacteriostatic water, 0.9% sodium chloride, or Phosphate-Buffered Saline (PBS, pH 7.4). After adding the desired volume of solvent, gentle swirling or inversion is recommended; vigorous vortexing or mechanical agitation should be avoided to prevent shear stress on the peptide backbone. Reconstituted stock solutions should be aliquoted into single-use microcentrifuge tubes to prevent repeated freeze-thaw cycles and maintained at 2°C to 8°C for short-term use (up to 7 days) or -20°C for extended experimental protocols.
PX1 Research Sourcing & Chain of Custody Standards
Procuring research reagents from PX1 Research guarantees full chain-of-custody control and lot traceability from initial solid-phase peptide synthesis (SPPS) through final freeze-drying and packaging. Our domestic synthesis facilities in the USA utilize automated peptide synthesizers and high-purity amino acid precursors to guarantee consistent step-wise yield and minimal chemical impurities.
Institutional laboratories requiring scalable supply for multi-phase preclinical trials can access specialized purchasing programs through our bulk peptide purchasing options. Whether ordering single analytical units or high-volume custom batches, PX1 Research maintains unwavering analytical standards, supporting the broader scientific community with reliable reagents backed by complete transparency.
Frequently Asked Questions
How do I verify the purity of Epitalon when buying online?
Always request a lot-specific Certificate of Analysis (COA) generated by an independent ISO 17025-accredited laboratory. The COA must include RP-HPLC chromatograms showing >99% purity and Mass Spectrometry data confirming the correct molecular mass (390.35 Da).
What is the primary mechanism of Epitalon in cell culture research?
In vitro research indicates that Epitalon acts as a peptide bioregulator that upregulates human telomerase reverse transcriptase (hTERT) gene expression, leading to telomerase activation, telomere elongation, and altered chromatin accessibility.
How should lyophilized Epitalon be stored upon delivery?
Lyophilized Epitalon should be stored at -20°C or -80°C in a dry, dark environment. Under these desiccated freezer conditions, the peptide remains stable for up to 24 months.
What solvents are suitable for reconstituting Epitalon in a lab context?
Epitalon is freely soluble in aqueous buffers. Suitable reconstitution solvents include sterile bacteriostatic water, sterile normal saline (0.9% NaCl), or standard Phosphate-Buffered Saline (PBS, pH 7.4) depending on downstream cell culture or assay requirements.
What endotoxin levels are acceptable for Epitalon in cell-based assays?
To prevent non-specific immune activation or cellular toxicity in sensitive assays, Epitalon should feature verified endotoxin levels below 0.1 EU/mg (PX1 Research enforces a strict standard of <0.01 EU/mg).
How does Epitalon differ from Thymalin in preclinical studies?
While Epitalon is a tetrapeptide focused on pineal gland function, melatonin synthesis, and telomerase expression, Thymalin is a thymic peptide complex primarily researched for T-cell differentiation and immune system modulation.
What distribution standards does PX1 Research utilize for peptide orders?
PX1 Research dispatches all peptide orders directly from US facilities in California and Arizona. Orders placed Monday through Friday before cut-off times qualify for same-day dispatch in temperature-controlled protective packaging.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.