Epithalon (Epitalon) is a synthetic tetrapeptide engineered to replicate the functional activity of epithalamin, a natural bioregulator isolated from the pineal gland. Designed strictly for in vitro assays and preclinical laboratory research, this compound is evaluated for its influence on telomerase expression, chromatin structure, and neuroendocrine signaling. PX1 Research provides analytical-grade Epithalon 10mg backed by lot-specific third-party testing and complete documentation.
Epithalon (Epitalon) is a synthetic tetrapeptide engineered to replicate the functional activity of epithalamin, a natural bioregulator isolated from the pineal gland. Designed strictly for in vitro assays and preclinical laboratory research, this compound is evaluated for its influence on telomerase expression, chromatin structure, and neuroendocrine signaling. PX1 Research provides analytical-grade Epithalon 10mg backed by lot-specific third-party testing and complete documentation.
When purchasing Epithalon 10mg for laboratory applications, principal investigators require high-purity synthetic tetrapeptide (Ala-Glu-Asp-Gly) with verified structural integrity. Epithalon is an established peptide bioregulator studied extensively for its role in telomerase activation, telomere length maintenance, and circadian rhythm regulation in cellular and animal models. PX1 Research provides researchers with high-grade Epithalon 10mg manufactured in compliance with strict USA quality standards.
To ensure precise experimental reproducible data, every batch of Epithalon 10mg distributed by PX1 Research undergoes rigorous testing in an ISO 17025 accredited laboratory. Each vial includes independent lot-specific analytical reports detailing chemical purity via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), molecular identity via Mass Spectrometry (MS), and low endotoxin thresholds suited for cell culture and preclinical bioassays.
Epithalon (also referred to as Epitalon or L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine) is a short synthetic peptide comprising four amino acids: L-Alanine, L-Glutamic acid, L-Aspartic acid, and Glycine. With a molecular formula of C16H26N4O9 and a molecular mass of approximately 418.4 g/mol, its low molecular weight facilitates rapid cellular uptake and nuclear interaction in preclinical models.
Discovered during studies investigating pineal gland extracts, Epithalon was designed to mimic the bioactivity of endogenous pineal peptides without the batch-to-batch variability inherent in crude tissue extractions. As a class representative of short-chain peptide bioregulators, Epithalon interacts directly with specific regions of genomic DNA, initiating targeted gene expression and chromatin remodeling without requiring complex membrane-bound receptor cascades.
The primary mechanism of Epithalon centers on its interaction with the catalytic subunit of the enzyme telomerase (human telomerase reverse transcriptase, or hTERT, in human cell lines). In vitro assays demonstrate that Epithalon induces the reactivation of telomerase in somatic cells where the gene is typically silent. By promoting telomerase expression, Epithalon facilitates the addition of repetitive TTAGGG nucleotide sequences to chromosome ends, counteracting progressive telomere shortening observed during successive mitotic divisions.
Beyond direct telomerase activation, preclinical literature indicates that Epithalon alters chromatin accessibility. By binding directly to histone proteins and promoter regions of specific genes, the tetrapeptide promotes de-heterochromatinization—unspooling tightly packed DNA to allow transcription factors access to promoter sites. This epigenetic modulation supports cellular protein synthesis, restores juvenile cell morphology, and delays premature cellular senescence in culture models. For further reading on cellular longevity mechanisms, explore our comprehensive research library hub.
The pineal gland serves as the primary regulator of circadian entrainment via the rhythmic secretion of melatonin. Preclinical models of accelerated aging and pineal involution demonstrate that administration of Epithalon normalizes night-time melatonin synthesis. In aged rodent models, Epithalon restores the sensitivity of the hypothalamus to hormonal feedback loops, modulating the hypothalamic-pituitary-adrenal (HPA) axis and stabilizing serum gonadotropin and corticosteroid fluctuations.
In vitro studies using pineal explants show that Epithalon stimulates the expression of arylalkylamine N-acetyltransferase (AANAT), the rate-limiting enzyme in melatonin biosynthesis. By restoring endogenously regulated melatonin peaks, research models demonstrate improvements in mitochondrial oxidative phosphorylation, decreased reactive oxygen species (ROS) accumulation, and enhanced synchronization of peripheral circadian clocks.
Preclinical investigations spanning several decades have evaluated the impact of Epithalon on cellular lifespan and organismal longevity in animal models. In vitro experiments on human diploid fibroblast cultures indicate that the addition of Epithalon extends the Hayflick limit—the maximum number of cell divisions possible prior to senescent arrest—by approximately 33% to 42% compared to untreated control groups.
In vivo rodent assays evaluating long-term administration of Epithalon report a reduction in spontaneous tumor incidence, enhanced immune cell proliferation (particularly T-cell maturation within thymic tissue), and an extension of average lifespan. Researchers studying telomerase activation peptides utilize Epithalon 10mg to examine how maintenance of chromosomal structural stability impacts systemic biomarker profiles, DNA damage response pathways (such as gamma-H2AX foci), and cellular repair mechanisms under conditions of induced oxidative stress.
When designing comparative protocols in longevity and cellular biology, researchers frequently benchmark Epithalon against other bioregulatory and mitochondrial peptides. Understanding the functional differences between these molecules allows investigators to target distinct biochemical signaling pathways.
While Epithalon acts primarily as a nuclear transcription modulator and telomerase activator, GHK-Cu 50mg functions chiefly as a copper-binding tripeptide focused on extracellular matrix remodeling, tissue repair, and gene expression resetting in skin and connective tissues. Conversely, MOTS-c 10mg is a mitochondria-derived peptide that regulates metabolic homeostasis, AMP-activated protein kinase (AMPK) expression, and insulin sensitivity. Additionally, compounds like Thymalin target T-cell differentiation within the immune system, whereas Epithalon displays broader pineal and chromosomal actions. Combining or comparing these agents in preclinical designs enables comprehensive profiling of distinct cellular repair mechanisms.
Analytical integrity is vital when sourcing research peptides for in vitro or preclinical study. PX1 Research adheres to stringent quality control mandates to ensure batch uniformity, biochemical stability, and freedom from cell-culture contaminants.
Every lot of Epithalon 10mg undergoes Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) to verify chromatographic purity exceeds 98.0%. Electrospray Ionization Mass Spectrometry (ESI-MS) confirms exact mass identity (418.4 Da) and rules out truncated peptide sequences or residual protecting groups. Furthermore, Chromogenic Limulus Amebocyte Lysate (LAL) testing guarantees endotoxin levels remain strictly below regulatory thresholds, preventing non-specific inflammatory signaling in cell culture or animal models. Laboratories managing high-volume projects can learn more about bulk purchasing via our wholesale portal.
Epithalon 10mg is supplied as a lyophilized (freeze-dried) sterile cake inside sealed glass vials. To preserve peptide stability, lyophilisate should be stored upon arrival at -20°C in a desiccated environment, shielded from direct light exposure.
For reconstitution, laboratory personnel should introduce an appropriate sterile diluent, such as Bacteriostatic Water (0.9% benzyl alcohol) or sterile 0.9% Sodium Chloride solution, allowing the diluent to run gently down the inner glass wall of the vial. Avoid vigorous agitation or shaking, as shearing forces can degrade peptide tertiary structure; gentle swirling is recommended to achieve complete dissolution. Once reconstituted, stock solutions should be aliquoted into single-use microcentrifuge tubes to eliminate freeze-thaw cycles and maintained at 2°C to 8°C for short-term use, or -80°C for extended storage protocols.
PX1 Research is dedicated to supporting scientific discovery by supplying North American laboratories with ultra-pure research peptides. Our facilities comply with cGMP principles, ensuring robust standard operating procedures across synthesis, purification, lyophilization, and secondary packaging.
When you choose PX1 Research, you receive full transparency. Every shipment includes batch-specific Certificates of Analysis (COAs) accessible online, verifying identity, purity, and safety metrics. Orders ship directly from our California and Arizona fulfillment centers with same-day dispatch for orders placed before cutoff times, ensuring your research schedule proceeds without unnecessary delay.
What is the purity level of PX1 Research Epithalon 10mg?
PX1 Research guarantees an RP-HPLC verified purity of ≥98.0% for every batch of Epithalon 10mg. Complete mass spectrometry data and lot-specific Certificates of Analysis are publicly provided with each shipment.
What is the recommended reconstitution solvent for Epithalon 10mg?
Epithalon 10mg reconstitutes readily in sterile Bacteriostatic Water or 0.9% Sodium Chloride Injection. The choice of solvent depends on the specific requirements of your in vitro assay or animal study protocol.
How should lyophilized Epithalon 10mg be stored prior to reconstitution?
Unopened lyophilized vials should be stored at -20°C in a dry, dark location. Under these conditions, the peptide remains stable for up to 24 months from the date of manufacture.
How does Epithalon interact with telomeres in laboratory models?
Preclinical data show that Epithalon induces the transcription of the catalytic subunit of telomerase (TERT), leading to enzyme activity that adds TTAGGG repeat sequences to chromosome ends, preserving telomere length during cell division.
Is Epithalon 10mg tested for endotoxins?
Yes. Every production lot of Epithalon 10mg undergoes LAL endotoxin testing to ensure levels remain below established safety thresholds (<0.1 EU/mg), preventing non-specific immune responses in experimental models.
Where is PX1 Research Epithalon 10mg manufactured and shipped from?
All PX1 Research peptides are manufactured in US-based GMP-compliant facilities and dispatched directly from our distribution hubs in California and Arizona.
Can Epithalon stock solutions undergo multiple freeze-thaw cycles?
Repeated freeze-thaw cycles can cause physical degradation and reduce peptide concentration. Reconstituted stock solutions should be aliquoted into single-use volumes before freezing at -80°C.
Is Epithalon approved for clinical or therapeutic use in humans?
No. Epithalon 10mg is strictly a research chemical designated for laboratory, in vitro, and preclinical animal research applications only. It is not intended for human consumption or clinical administration.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.