Buy Hexarelin Acetate
To buy Hexarelin Acetate for laboratory research, investigators require a verified vendor capable of providing high-purity, analytical-grade material with transparent lot-specific documentation. Hexarelin Acetate is a potent synthetic hexapeptide growth hormone secretagogue receptor (GHSR-1a) agonist and CD36 ligand studied extensively in preclinical models of endocrine regulation, cardiac ischemia, and tissue preservation. PX1 Research supplies USA-manufactured Hexarelin Acetate with verified mass spectrometry, HPLC purity exceeding 98%, and certified low endotoxin levels for reliable in vitro and ex vivo experimental workflows.
Quick answer
To buy Hexarelin Acetate for laboratory research, investigators require a verified vendor capable of providing high-purity, analytical-grade material with transparent lot-specific documentation. Hexarelin Acetate is a potent synthetic hexapeptide growth hormone secretagogue receptor (GHSR-1a) agonist and CD36 ligand studied extensively in preclinical models of endocrine regulation, cardiac ischemia, and tissue preservation. PX1 Research supplies USA-manufactured Hexarelin Acetate with verified mass spectrometry, HPLC purity exceeding 98%, and certified low endotoxin levels for reliable in vitro and ex vivo experimental workflows.
Key takeaways
- [Hexarelin](/research-peptides/hexarelin) Acetate (CAS 140703-51-1) is a synthetic hexapeptide with the chemical sequence His-D-2-Me-Trp-Ala-Trp-D-Phe-Lys-NH2.
- Preclinical investigations demonstrate that [Hexarelin](/research-peptides/hexarelin) acts as a high-affinity full agonist at the growth hormone secretagogue receptor type 1a (GHSR-1a), a 7-transmembrane G-protein coupled receptor (GPCR) predominantly expressed in the anterior pituitary gland and hypothalamus.
- A distinct feature of [Hexarelin](/research-peptides/hexarelin) Acetate in contrast to many other [growth hormone secretagogues](/research-peptides/growth-hormone-secretagogues) is its functional interaction with CD36 (thrombospondin receptor), a scavenger receptor protein expressed on cardiomyocytes, vascular endothelial cells, and macrophages.
- Within the growth hormone secretagogue class, peptides vary substantially in receptor potency, selectivity, desensitization rates, and off-target hormone release.
Chemical Identification and Structural Overview of Hexarelin Acetate
Hexarelin Acetate (CAS 140703-51-1) is a synthetic hexapeptide with the chemical sequence His-D-2-Me-Trp-Ala-Trp-D-Phe-Lys-NH2. As a structural analogue of GHRP-6, Hexarelin incorporates a modified D-2-methyl-tryptophan residue at position 2, which significantly enhances its enzymatic resistance and receptor affinity compared to first-generation secretagogues. In its acetate salt formulation, the compound demonstrates high aqueous solubility, making it exceptionally suited for controlled reconstituted liquid assays in vitro.
The molecular weight of the free base peptide is approximately 887.04 g/mol, with an empirical formula of C47H58N12O6. In molecular research, the incorporate of specific D-amino acids stabilizes the peptide chain against cleavage by circulating aminopeptidases and endopeptidases. When preparing experimental protocols, researchers evaluating hexarelin acetate must account for these structural modifications, as they directly influence binding kinetics, half-life parameters, and target specificity during receptor ligand assays.
Molecular Mechanism of Action: GHSR-1a Activation
Preclinical investigations demonstrate that Hexarelin acts as a high-affinity full agonist at the growth hormone secretagogue receptor type 1a (GHSR-1a), a 7-transmembrane G-protein coupled receptor (GPCR) predominantly expressed in the anterior pituitary gland and hypothalamus. Upon ligand binding, GHSR-1a initiates a Gq/11 protein-mediated intracellular signaling cascade. This activation stimulates phospholipase C (PLC), leading to the cleavage of phosphatidylinositol 4,5-bisphosphate (PIP2) into inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG).
The generation of IP3 triggers the rapid release of stored intracellular calcium ions (Ca2+) from the endoplasmic reticulum into the cytoplasm of somatotroph cells. Simultaneously, DAG activates protein kinase C (PKC). In rodent cell culture models, this dual signaling pathway drives the exocytosis of pre-stored growth hormone granules. Unlike endogenous growth hormone-releasing hormone (GHRH), which operates via the cyclic AMP (cAMP) / protein kinase A (PKA) pathway, Hexarelin operates independently of GHRH receptors, demonstrating a synergistic somatotroph response when co-administered with GHRH analogues in preclinical research.
Secondary Target Signaling: CD36 Binding and Cardiovascular In Vitro Models
A distinct feature of Hexarelin Acetate in contrast to many other growth hormone secretagogues is its functional interaction with CD36 (thrombospondin receptor), a scavenger receptor protein expressed on cardiomyocytes, vascular endothelial cells, and macrophages. In vitro receptor binding studies confirm that Hexarelin binds CD36 with nanomolar affinity, independent of its GHSR-1a binding capability.
In ex vivo rodent heart perfusion models (Langendorff preparations), Hexarelin administration has been observed to mitigate myocardial ischemia-reperfusion injury. Researchers have documented that this protective effect persists even in hypophysectomized animal models where systemic growth hormone secretion is completely absent. The activation of CD36 signaling pathways by Hexarelin correlates with reduced apoptotic signaling, altered fatty acid transport kinetics, and enhanced cardiomyocyte survival under hypoxic conditions, establishing it as a primary candidate for cardiovascular tissue preservation research.
Comparative Analysis: Hexarelin vs. GHRP-2, GHRP-6, and Ipamorelin
Within the growth hormone secretagogue class, peptides vary substantially in receptor potency, selectivity, desensitization rates, and off-target hormone release. Laboratory evaluation frequently requires comparative benchmarking against related hexapeptides and pentapeptides to establish baseline signal-to-noise ratios in receptor assays.
Preclinical binding studies indicate that Hexarelin exhibits higher intrinsic activity at GHSR-1a than ghrp-6, resulting in a significantly greater peak magnitude of intracellular calcium mobilization. However, while ipamorelin displays extreme selectivity for GHSR-1a without elevating cortisol or prolactin levels in rodent models, Hexarelin induces a modest transient activation of ACTH and prolactin pathways at elevated concentrations. Compared to ghrp-2, Hexarelin demonstrates unique receptor binding kinetics at CD36, making it distinct among GHSR agonists. Researchers seeking maximum somatotroph receptor stimulation alongside cardiovascular marker monitoring frequently choose Hexarelin, whereas those requiring strict receptor selectivity without desensitization often investigate Ipamorelin or cjc-1295-no-dac.
Preclinical Findings in Metabolic, Muscle, and Tissue Preservation Studies
In animal models of catabolic muscle wasting and cachexia, preclinical research shows that Hexarelin administration helps attenuate protein degradation in skeletal muscle tissue. In vitro myoblast assays suggest that Hexarelin modulates up-regulation of the Akt/mTOR signaling pathway, concurrently down-regulating muscle-specific ubiquitin ligases such as MuRF-1 and Atrogin-1 under catabolic stress.
Furthermore, rodent metabolic studies indicate that Hexarelin plays a role in lipid metabolism modulation and systemic insulin sensitivity. Investigators examining bone mineral density parameters in animal models have reported elevated osteoblast proliferation markers following Hexarelin exposure. These multifactorial tissue responses highlight the peptide's broad utility across physiological research disciplines, ranging from cellular aging assays to musculoskeletal regeneration studies.
Receptor Tachyphylaxis and Desensitization Dynamics
A critical parameter in designing long-term in vitro or in vivo experimental protocols with Hexarelin Acetate is its propensity for receptor tachyphylaxis. Repeated continuous exposure of GHSR-1a to high concentrations of Hexarelin leads to rapid receptor phosphorylation, beta-arrestin recruitment, and subsequent receptor internalization.
In preclinical rodent trials, daily administration of Hexarelin results in a progressive dampening of peak growth hormone response over a 14-day to 28-day timeline—a phenomenon far more pronounced than that observed with Ipamorelin. Researchers investigating long-term receptor dynamics must incorporate pulsatile treatment regimens or designated washout periods within their experimental designs to maintain GHSR-1a responsiveness and avoid persistent receptor downregulation.
Reconstitution, Handling, and Storage Protocols for Laboratory Use
Hexarelin Acetate is supplied as a sterile, lyophilized white powder to ensure long-term chemical stability. To preserve molecular integrity prior to experimental application, laboratory researchers must strictly observe proper handling, reconstitution, and storage protocols:
1. Reconstitution Medium: Lyophilized Hexarelin should be reconstituted using sterile bacteriostatic water (0.9% benzyl alcohol) or sterile laboratory-grade phosphate-buffered saline (PBS, pH 7.4), depending on the requirements of the downstream cell culture or analytical assay. 2. Reconstitution Technique: Allow the vial to equalize to room temperature before inserting the needle. Slowly direct the diluent down the glass inner wall of the vial. Gently swirl the container until the cake completely dissolves. Never shake or vortex the solution, as mechanical shear stress can break delicate peptide bonds and lead to aggregation. 3. Concentration Calculation: Reconstitute to a standard laboratory working concentration (e.g., 1 mg/mL or 2 mg/mL) to ensure accurate micro-pipetting during serial dilutions. 4. Temperature Storage Parameters: Store lyophilized powder at -20°C for long-term stability (up to 24 months). Following reconstitution, store liquid aliquots at 2°C to 8°C for short-term use (up to 28 days) or sub-aliquot into single-use polypropylene tubes and freeze at -80°C to eliminate destructive freeze-thaw cycles.
Quality Verification: Analytical Testing Standards for Hexarelin Sourcing
When procurement officers and principal investigators prepare to buy hexarelin acetate, verifying the analytical purity of the compound is paramount to experimental reproducibility. Subtle contaminants, unprotecting side-products, or heavy metal residues can alter cell culture viability, distort receptor binding assays, and invalidate published data.
A legitimate research supplier must validate every production batch through an independent, ISO 17025-accredited laboratory using three core analytical techniques: - Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC): Confirms chromatographic purity, verifying that the main target peak represents >98% of total integrated UV absorbance. - Electrospray Ionization Mass Spectrometry (ESI-MS): Verifies the exact molecular mass (~887.04 Da) and molecular fingerprint, ruling out sequence mutations or truncation peptides. - Limulus Amebocyte Lysate (LAL) Endotoxin Testing: Measures bacterial endotoxin levels. For cellular assays, endotoxin concentrations must remain strictly below 0.01 EU/mg to prevent unintended inflammatory responses in cell culture media.
Why Procure Hexarelin Acetate from PX1 Research
PX1 Research is an established USA-based manufacturer and distributor of high-grade research peptides, dedicated exclusively to supporting academic institutions, private research laboratories, and biotechnology firms. We enforce strict quality control measures to ensure that every vial of Hexarelin Acetate meets rigorous chemical specifications.
Every batch of Hexarelin Acetate available through our catalog includes a downloadable, lot-specific Certificate of Analysis (COA) generated by an independent third-party laboratory. Our compounds are synthesized in state-of-the-art cGMP-compliant facilities across California and Arizona. Orders placed before cut-off times ship same-day (Monday through Friday) in specialized protective packaging, minimizing thermal degradation during transport. Whether procuring small quantities for preliminary assays or establishing a wholesale laboratory account for high-throughput screening, PX1 Research delivers unmatched purity, lot-to-lot consistency, and analytical transparency.
Frequently Asked Questions
What is the primary target receptor of Hexarelin Acetate?
Hexarelin Acetate acts primarily as a full agonist at the growth hormone secretagogue receptor 1a (GHSR-1a). Secondarily, it binds with high affinity to the CD36 scavenger receptor, making it valuable for cardiovascular and metabolic in vitro research.
What analytical purity level is guaranteed for PX1 Research Hexarelin Acetate?
PX1 Research guarantees a minimum of 98% chemical purity for Hexarelin Acetate, verified by independent third-party Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) and Electrospray Ionization Mass Spectrometry (ESI-MS).
Is Hexarelin Acetate stable at room temperature?
In its lyophilized (powder) form, Hexarelin Acetate is stable at room temperature for brief shipping periods. However, upon arrival at the laboratory, it should immediately be stored at -20°C for long-term stability.
How does Hexarelin differ from Ipamorelin in preclinical assays?
Hexarelin is a more potent GHSR-1a agonist with unique CD36 receptor binding, but it undergoes faster receptor desensitization (tachyphylaxis) and may cause minor elevations in ACTH/prolactin at high doses. Ipamorelin is highly selective for GHSR-1a and does not cause ACTH or prolactin release.
How should reconstituted Hexarelin Acetate be stored?
Reconstituted liquid Hexarelin should be stored at 2°C to 8°C for up to 28 days. For extended experimental timelines, solution aliquots should be frozen at -80°C to prevent degradation and avoid repeated freeze-thaw cycles.
What diluent is recommended for reconstituting Hexarelin Acetate?
For standard laboratory procedures, sterile bacteriostatic water (containing 0.9% benzyl alcohol) or sterile phosphate-buffered saline (PBS) is recommended, depending on whether the compound will be used in multi-use aliquots or immediate cell culture assays.
Does PX1 Research provide a Certificate of Analysis (COA) with each order?
Yes. Every lot of Hexarelin Acetate supplied by PX1 Research includes a downloadable, lot-specific COA detailing HPLC purity, ESI-MS mass verification, and LAL endotoxin testing data from an accredited ISO 17025 laboratory.
Can Hexarelin Acetate be ordered in bulk for institutional accounts?
Yes. PX1 Research offers tiered pricing and dedicated support for institutional research procurement, academic grants, and enterprise accounts through our wholesale research portal.
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