PX1 Research supplies USA-manufactured KPV 10mg lyophilized powder synthesized for rigorous in vitro and preclinical research applications. Every lot undergoes independent analytical testing via HPLC and Mass Spectrometry to guarantee >98% purity, paired with comprehensive endotoxin screening.
PX1 Research supplies USA-manufactured KPV 10mg lyophilized powder synthesized for rigorous in vitro and preclinical research applications. Every lot undergoes independent analytical testing via HPLC and Mass Spectrometry to guarantee >98% purity, paired with comprehensive endotoxin screening.
When purchasing laboratory-grade reagents, researchers looking to buy KPV 10mg require verified purity, molecular stability, and complete lot traceability. KPV (Lysine-Proline-Valine) is a synthesized C-terminal tripeptide fragment of alpha-melanocyte-stimulating hormone (α-MSH). Available from PX1 Research as a single-vial 10mg lyophilized cake, this compound is dedicated strictly to laboratory experimentation and analytical assays.
PX1 Research ensures that every batch of KPV 10mg manufactured in our domestic GMP-compliant facilities meets stringent specifications. Laboratory procurement teams gain access to lot-specific Certificates of Analysis (COAs) validating peptide content, purity profiles via High-Performance Liquid Chromatography (HPLC), mass confirmation via Mass Spectrometry (MS), and bioburden limits. All orders ship directly from our California and Arizona distribution hubs with same-day handling for orders placed Monday through Friday.
KPV is a tripeptide composed of the amino acid sequence L-Lysyl-L-prolyl-L-valine (Lys-Pro-Val). Representing residues 11–13 of the endogenous peptide hormone α-MSH, KPV retains key anti-inflammatory signalling properties of its parent molecule while lacking the melanogenic activities associated with full-length melanocortin receptor activation.
With a low molecular weight of approximately 341.45 g/mol, the KPV tripeptide exhibits favorable solubility and transport characteristics in aqueous media. Investigators frequently explore KPV in cellular models due to its ability to cross epithelial barriers via specialized transport machinery, such as the solute carrier family 15 member 1 (PepT1) transporter. For detailed structural data and physical parameters, researchers can consult our research library hub or review specific compound listings in the research peptide catalog.
In vitro and ex vivo studies suggest that KPV acts primarily as a down-regulator of pro-inflammatory signaling cascades. The main target of interest in preclinical literature is the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. NF-κB serves as a primary transcription factor governing the expression of pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6.
Preclinical models demonstrate that KPV can enter epithelial and immune cells to inhibit the nuclear translocation of p65, a functional subunit of NF-κB. By preventing this nuclear translocation, KPV attenuates the downstream transcription of inflammatory mediators. Additionally, research utilizing cell culture assays indicates that KPV may interact with intracellular importin proteins, thereby physically impeding the nuclear import machinery responsible for NF-κB activation during cellular stress.
A central focus of contemporary research involving KPV is its effect on mucosal immunology and intestinal epithelial dynamics. In rodent models of experimental colitis (such as dextran sulfate sodium [DSS]-induced and trinitrobenzene sulfonic acid [TNBS]-induced mucosal injury), administration of KPV has been shown to reduce inflammatory histological scores and preserve mucosal architecture.
Mechanistic research highlights the role of the oligopeptide transporter PepT1, which is often upregulated in inflamed intestinal tissues. Preclinical evidence indicates that KPV uses PepT1 transport to cross apical membranes in Caco-2 monolayer cell models. Inside enterocytes, KPV reduces oxidative stress markers and preserves tight junction protein expression (such as ZO-1 and Occludin), supporting the structural integrity of the intestinal epithelial barrier. Researchers studying gastrointestinal pathologies often cross-reference these findings with broader gastrointestinal epithelial integrity research.
In mucosal preservation and cellular repair studies, KPV is frequently evaluated alongside other candidate research molecules. While KPV specifically targets intracellular NF-κB translocation via PepT1 transport, compounds like BPC-157 10mg operate primarily through growth factor receptor upregulation, focal adhesion kinase signaling, and angiogenic modulation in wounded tissue models.
Similarly, Larazotide research parameters center on tight junction assembly by acting as a zonulin antagonist, preventing tight junction disassembly without directly inhibiting intracellular cytokine signaling. Another related compound in cellular repair studies, TB-500 10mg, functions via actin sequestration and cell migration pathways. Investigating these distinct mechanisms allows researchers to design comparative assays targeting distinct pathways within gastrointestinal barrier dynamics.
To ensure reproducible experimental results, laboratory personnel require rigorous proof of compound identity and purity. Every lot of KPV 10mg research vial supplied by PX1 Research is subjected to strict analytical verification in an ISO 17025 accredited laboratory environment.
Analytical verification protocols for PX1 KPV include:
• Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Establishes chemical purity, ensuring freedom from baseline degradation products or truncated peptide sequences, with a target threshold of ≥98.0% purity.
• Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms exact molecular mass match to the theoretical molecular weight of Lys-Pro-Val.
• Endotoxin Quantitation (LAL Assay): Evaluates bacterial endotoxin content to guarantee levels remain well below standard cellular assay toxicity thresholds (<0.5 EU/mg).
• Residual Solvent Analysis: Verifies total removal of synthesis solvents (such as TFA and acetonitrile) through lyophilization. Institutional facilities seeking high-volume lots can review our bulk analytical compound programs.
KPV is supplied as a sterile, lyophilized cake inside a sealed glass vial. Reconstitution should always occur within a certified laminar flow hood using sterile laboratory practices to maintain solution integrity and avoid microbial contamination.
For most cell culture and biochemical protocols, sterile Bacteriostatic Water, Sterile Water for Injection (WFI), or phosphate-buffered saline (PBS, pH 7.4) may be utilized as a solvent. The tripeptide exhibits high solubility in aqueous buffer systems. After introducing the solvent along the inner glass wall of the vial, gently swirl the container until the lyophilized powder dissolves completely. Avoid vigorous vortexing, which can induce mechanical shear stress or foam formation. Once reconstituted, stock solutions should be aliquoted into single-use polypropylene microtubes to prevent degradation caused by repeated freeze-thaw cycles.
Lyophilized KPV 10mg should be stored in a freezer at -20°C upon receipt for optimal long-term stability. Unopened vials kept under desiccation at -20°C maintain chemical integrity for up to 24 months from the date of synthesis.
Reconstituted stock solutions prepared in sterile aqueous buffers should be used immediately or stored at -20°C to -80°C for short-term experimental series. Reconstituted liquid aliquots stored at 2°C to 8°C should be utilized within 7 to 14 days to prevent progressive hydrolytic degradation. Always protect stored solutions from prolonged light exposure. For additional handling protocols regarding melanocortin-derived fragments, consult our technical resource on alpha-MSH signaling pathways.
PX1 Research streamlines procurement for university laboratories, biotechnology enterprises, and institutional buyers. When you buy KPV 10mg through our platform, your order is dispatched from our state-of-the-art dispatch hubs located in California and Arizona. Orders processed before cutoff times Monday through Friday ship same-day.
Vials are packaged in protective temperature-controlled insulation to ensure physical stability during transit. Institutional purchasing managers requiring custom batch sizes, bulk pricing, or specialized analytical documentation can easily manage orders through our wholesale laboratory account portal. All compounds offered remain strictly for laboratory research use only.
What is the purity specification for PX1 Research KPV 10mg?
PX1 Research KPV 10mg is verified by third-party RP-HPLC to meet or exceed 98.0% purity. Each lot includes a downloadable Certificate of Analysis confirming purity and mass identity.
Is KPV 10mg intended for human consumption or therapeutic use?
No. KPV 10mg is sold strictly for in vitro and preclinical laboratory research use. It is not approved for human consumption, therapeutic use, veterinary use, or clinical administration.
How should reconstituted KPV stock solutions be stored?
Reconstituted KPV solutions should be divided into single-use aliquots and stored at -20°C or -80°C to avoid repeated freeze-thaw cycles. Short-term storage at 2°C–8°C is acceptable for up to 14 days.
What solvent is recommended for reconstituting KPV 10mg?
KPV is readily soluble in sterile water for injection, bacteriostatic water, or standard phosphate-buffered saline (PBS, pH 7.4) depending on the specific requirements of your assay.
How is endotoxin content measured for KPV batches?
Every lot of KPV undergoes Limulus Amebocyte Lysate (LAL) testing to ensure endotoxin levels remain below standard analytical thresholds (typically <0.5 EU/mg).
Where does PX1 Research ship KPV orders from?
All orders are fulfilled directly from our facility hubs located in California and Arizona, with same-day shipping available for orders placed Monday through Friday.
What mechanism does KPV exhibit in cellular inflammation assays?
Preclinical studies show KPV enters cells via PepT1 transporters to inhibit NF-κB p65 nuclear translocation, thereby reducing the transcription of pro-inflammatory cytokines.
Can I obtain a lot-specific COA before or after purchasing KPV 10mg?
Yes, PX1 Research provides batch-specific COAs displaying HPLC chromatograms and Mass Spec outputs directly on our site or upon request by lab administrators.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.