PNC-27 is a specialized synthetic research peptide consisting of the p53 residue 12–26 sequence coupled to a cell-penetrating leader domain. Designed exclusively for non-clinical cell culture and biochemical assays, PNC-27 is investigated for its selective binding affinity to membrane-localized HDM-2 protein and subsequent pore-forming cytotoxic mechanisms. PX1 Research delivers laboratory-grade PNC-27 featuring third-party analytical verification and rapid domestic shipping for qualified academic and institutional labs.
PNC-27 is a specialized synthetic research peptide consisting of the p53 residue 12–26 sequence coupled to a cell-penetrating leader domain. Designed exclusively for non-clinical cell culture and biochemical assays, PNC-27 is investigated for its selective binding affinity to membrane-localized HDM-2 protein and subsequent pore-forming cytotoxic mechanisms. PX1 Research delivers laboratory-grade PNC-27 featuring third-party analytical verification and rapid domestic shipping for qualified academic and institutional labs.
If your laboratory needs to buy PNC-27 peptide for experimental assays, sourcing verified high-purity reagents is critical to achieving reproducible, publishable results. Researchers seeking to buy PNC-27 peptide must ensure the compound is manufactured under strict quality standards, free of unreacted precursor sequences, residual TFA salts, and microbial endotoxins. PX1 Research serves as a trusted primary vendor for academic institutions, biotechnology firms, and analytical laboratories requiring reference-grade research peptides.
When evaluating pnc-27 peptide where to buy, primary investigators must inspect third-party analytical data rather than relying on vendor claims. PX1 Research ships every lot of PNC-27 directly from fully compliant facilities in California and Arizona. Each order includes a comprehensive Certificate of Analysis (COA) detailing Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) purity analysis and Electrospray Ionization Mass Spectrometry (ESI-MS) identity verification, guaranteeing that researchers receive exact chemical specifications for in vitro testing.
PNC-27 is a 32-amino-acid synthetic construct comprised of two distinct functional regions. The main targeting domain consists of residues 12 through 26 of the human p53 tumor suppressor protein (P-P-Q-T-F-S-N-L-W-K-L-L-K-K-L). This sequence is synthetically conjugated at the carboxyl terminus to a 17-amino-acid transmembrane-penetrating peptide derived from the Antennapedia homeodomain, commonly referred to as the penetratin leader sequence.
In physical chemistry assays, the peptide exhibits a molecular weight of approximately 4,031.7 Da. The inclusion of the cell-penetrating peptide (CPP) tail allows PNC-27 to readily cross cellular membranes without requiring receptor-mediated endocytosis. For investigators looking to buy pnc 27 peptide for structural biology studies, understanding this dual-domain architecture is essential for modeling its interaction with target cell membranes and membrane-associated protein complexes.
Preclinical studies indicate that PNC-27 operates via a distinct membrane-active cytotoxic mechanism that sets it apart from traditional intracellular p53-pathway activators. In non-transformed, healthy cell lines, the HDM-2 (human double minute 2) protein resides predominantly in the nucleus and cytoplasm, where it acts as a negative regulator of internal p53. However, in various transformed and tumor-derived cell lines, HDM-2 is selectively expressed on the outer cell membrane.
In vitro data demonstrate that the p53-derived domain of PNC-27 specifically binds to the hydrophobic cleft of this membrane-bound HDM-2. Upon binding, the amphipathic alpha-helical structure of PNC-27 induces transmembrane pore formation, leading to rapid loss of membrane integrity, cell swelling, and necrotic cell lysis within hours. Crucially, this cytolytic mechanism functions independently of nuclear p53 transcriptional activity or apoptotic pathways, providing a novel framework for studying membrane-targeted cytotoxicity in cell lines resistant to apoptosis.
In cell culture models, researchers utilize PNC-27 to investigate selective membrane disruption across diverse cancer cell lines, including pancreatic carcinoma, melanoma, breast adenocarcinoma, and leukemia cultures. Because healthy, non-transformed cells do not present HDM-2 on their external surface membrane, in vitro studies repeatedly show that PNC-27 leaves non-malignant control cells undamaged at equivalent concentrations.
These non-clinical observations make PNC-27 a valuable tool in exploring targeted membrane-disrupting peptides (MDPs). Investigators cataloging cell viability, lactate dehydrogenase (LDH) release, and propidium iodide uptake use high-purity PNC-27 from our all peptides hub to establish accurate dose-response curves. Accessing detailed mechanism data through our research library allows scientists to contextualize PNC-27 activity alongside other membrane-active agents.
When designing comparative cytotoxic or cell-penetrating experiments, researchers frequently compare PNC-27 against structurally related peptides. Below is an overview of how PNC-27 compares to similar compounds in preclinical research models:
PNC-27 features the p53 (12-26) sequence conjugated to penetratin, targeting membrane HDM-2 to induce necrosis. In contrast, PNC-28 utilizes a shorter p53 segment (residues 17-26) attached to the same leader sequence, displaying similar membrane-disrupting characteristics but varying in binding kinetics and helical stability. Meanwhile, non-targeted cell-penetrating peptides or structural signaling research compounds like BPC-157 lack HDM-2 affinity entirely and are studied for distinct tissue response and cellular migration pathways. Evaluating these differences helps research teams choose the correct reference compound for targeted membrane lysis assays.
To ensure experimental repeatability when you buy PNC-27 peptide, your procurement protocol should mandate rigorous testing specifications. Low-purity peptide batches containing truncated sequences or unremoved synthesis reagents can confound cell culture assays, causing non-specific toxicity or false-positive results.
At PX1 Research, every production batch of PNC-27 is subjected to a strict quality protocol: high-resolution RP-HPLC confirms peptide purity exceeds 98%, ESI-MS verifies exact molecular weight, and chromogenic LAL assays ensure bacterial endotoxin levels remain below 0.01 EU/mg. Furthermore, our facilities utilize ISO 17025 accredited analytical testing to ensure lot-to-lot consistency across all research peptides.
PNC-27 is supplied as a lyophilized (freeze-dried) powder in sealed glass vials to ensure long-term chemical stability. To maintain compound integrity during in vitro preparation, laboratories should adhere to standard aseptic reconstitution guidelines:
1. Solvation: Allow the vial to equilibrate to room temperature before reconstitution to prevent condensation. Reconstitute using sterile, deionized water or phosphate-buffered saline (PBS, pH 7.4), depending on assay requirements. Gentle swirling is recommended; avoid vigorous vortexing to prevent peptide aggregation. 2. Aliquoting: Dilute to a stock concentration (e.g., 1 mg/mL) and aliquot into sterile, low-binding microcentrifuge tubes to prevent adsorption to container walls. 3. Storage: Store lyophilized vials at -20°C or -80°C for long-term stability. Reconstituted liquid stock aliquots should be kept at -80°C and subjected to minimal freeze-thaw cycles.
Timely availability of reagents is critical for maintaining research project timelines. PX1 Research operates state-of-the-art logistics nodes in California and Arizona, enabling same-day dispatch for orders placed before cutoff times Monday through Friday. Domestic fulfillment eliminates customs delays and thermal degradation risks associated with overseas transit.
For universities, contract research organizations (CROs), and institutional labs requiring bulk quantities, PX1 Research provides scalable supply solutions. Institutional buyers can apply for dedicated accounts and volume pricing structures through our streamlined wholesale portal.
pnc-27 peptide where to buy for laboratory research?
You can buy PNC-27 peptide directly from PX1 Research. We provide laboratory-grade PNC-27 synthesized in US-based GMP-compliant facilities with verified HPLC purity above 98% and lot-specific COAs included with every shipment.
What should I check before I buy pnc-27 peptide online?
When preparing to buy PNC-27 peptide, verify that the vendor provides lot-specific mass spectrometry (MS) and high-performance liquid chromatography (HPLC) reports, confirms endotoxin levels below 0.01 EU/mg, and ships directly from verified domestic logistics facilities.
How do I buy pnc 27 peptide for institutional lab use?
Institutional purchasing departments and principal investigators can buy PNC-27 peptide online using standard lab procurement cards or set up high-volume corporate accounts via our wholesale portal.
What is the specific target mechanism of PNC-27 in cell culture studies?
In preclinical cell culture models, PNC-27 selectively targets HDM-2 proteins expressed on the outer cell membrane of transformed cancer cells. Binding to membrane HDM-2 triggers transmembrane pore formation and cell lysis (necrosis) independent of p53 transcriptional activity.
Why does PNC-27 not harm normal, non-transformed cells in vitro?
In vitro studies demonstrate that non-transformed control cells express HDM-2 exclusively within the nucleus and cytoplasm, rather than on the external cell membrane. Lacking membrane-bound HDM-2 targets, healthy cells do not bind PNC-27 and remain viable.
How does PNC-27 differ from PNC-28?
PNC-27 utilizes the p53 residues 12-26 attached to a penetratin leader peptide, whereas PNC-28 utilizes residues 17-26 attached to the same leader peptide. Both are studied for membrane-disrupting capabilities, but differ in binding affinity and molecular weight.
How should PNC-27 be reconstituted for in vitro testing?
PNC-27 should be reconstituted under a laminar flow hood using sterile PBS or sterile bacteriostatic water. Gentle dissolution without excessive vortexing is recommended, followed by aliquoting into low-binding tubes for storage at -80°C.
Why is endotoxin testing critical when purchasing PNC-27?
Bacterial endotoxins (LPS) can induce non-specific inflammatory responses and cell death in culture, compromising the validity of cytotoxicity assays. PX1 Research tests every batch of PNC-27 to ensure endotoxins remain below 0.01 EU/mg.
Is PNC-27 approved for therapeutic use or human administration?
No. PNC-27 is an experimental research chemical supplied strictly for in vitro, biochemical, and non-clinical laboratory research. It is not approved by the FDA for human therapeutic, clinical, or diagnostic use.
What analytical documentation is provided when I buy PNC-27 from PX1 Research?
Every order of PNC-27 includes a lot-specific Certificate of Analysis (COA) containing RP-HPLC purity traces, ESI-MS mass confirmation data, and endotoxin assay results.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.