Vilon is a synthetic dipeptide (Lysyl-Glutamic acid) widely evaluated in preclinical literature for its capacity to modulate chromatin structure and regulate gene expression in cellular models. Designed exclusively for laboratory research, high-purity Vilon provides researchers with a targeted tool for investigating epigenetic mechanisms, thymic pathways, and cellular senescence.
Vilon is a synthetic dipeptide (Lysyl-Glutamic acid) widely evaluated in preclinical literature for its capacity to modulate chromatin structure and regulate gene expression in cellular models. Designed exclusively for laboratory research, high-purity Vilon provides researchers with a targeted tool for investigating epigenetic mechanisms, thymic pathways, and cellular senescence.
When qualified investigators seek to buy Vilon gene expression peptides for laboratory research, sourcing high-purity, fully verified material is essential for reproducible experimental outcomes. Vilon (Lys-Glu) is a synthetic short-chain bioregulatory dipeptide studied for its capacity to interact with DNA and histone complexes, influencing chromatin decondensation and gene transcription in vitro.
PX1 Research supplies research-grade Vilon dipeptide manufactured in USA-based, GMP-compliant facilities. Every lot undergoes rigorous analytical characterization—including reverse-phase high-performance liquid chromatography (RP-HPLC) for purity verification and electrospray ionization mass spectrometry (ESI-MS) for structural confirmation—ensuring exceptional quality for advanced genomic and immunogenetic assays.
Vilon is composed of two amino acid residues: L-lysine and L-glutamic acid (Lys-Glu). Originating from broader investigations into thymic peptide fractions, Vilon represents a minimal active motif capable of mimicking regulatory signals previously associated with larger polypeptide extracts. In molecular biology research, short-chain peptides like Vilon are evaluated for their direct interaction with the major and minor grooves of double-stranded DNA, as well as histone tails.
Preclinical studies suggest that Vilon modulates gene expression by binding to specific DNA sequences or nucleosome regions, facilitating local chromatin remodeling. By promoting heterochromatin decondensation (uncoiling tightly packed genomic regions), Vilon permits RNA polymerase complexes and transcription factors to access previously silenced gene loci. In vitro assays demonstrate that this structural alteration can upregulate structural and functional proteins associated with cellular homeostasis.
Extensive in vitro and animal models have documented Vilon's influence across several transcriptomic markers. Researchers investigating cellular senescence have observed that Vilon exposure in cultured fibroblast and lymphocyte lines correlates with altered expression profiles of key cytokines, heat shock proteins, and antioxidant enzymes. The ability of short peptides to reactivate transcription in heterochromatinized regions of aging cells forms the cornerstone of peptide bioregulation theory.
Furthermore, in vitro data indicate that Lys-Glu dipeptides alter the expression of genes involved in cell cycle regulation and apoptosis. In rodent models, researchers have analyzed tissue-specific mRNA levels following peptide administration, noting restored transcript levels for thymic and hepatic proteins that typically decline with age or environmental stress. Detailed mechanics of these transcriptomic shifts can be further explored within our peptide research library.
Historically derived from thymic peptide research, Vilon plays a significant role in preclinical immunology experiments. In vitro cell cultures of thymocytes and peripheral blood lymphocytes show altered interleukin-2 (IL-2) and interferon-gamma (IFN-γ) expression when treated with Lys-Glu dipeptides. These findings suggest a regulatory loop wherein short peptide signals influence immune cell differentiation and functional activation at the genomic level.
Investigators examining age-related thymic involution utilize Vilon to explore whether peptide-mediated chromatin uncoiling can restore functional marker expression in senescent immunocompetent cells. By maintaining transcriptional activity in thymic stromal elements, Vilon serves as a pivotal tool for unraveling non-genomic and epigenetic signaling cascades in modern immunobiology.
Vilon belongs to a broader class of short bioregulatory peptides investigated for sequence-specific gene regulation. To assist researchers in building targeted experimental protocols, it is useful to compare Vilon against other prominent short-chain research peptides:
While Epitalon (Ala-Glu-Asp-Gly) is predominantly studied for telomerase activation and pineal gland gene expression, and Livagen (Lys-Glu-Asp-Pro) focuses on chromatin structure in hepatic tissues, Vilon (Lys-Glu) offers a ultra-short dipeptide motif primarily associated with thymic immune signaling and generalized chromatin decondensation. Researchers comparing these compounds often evaluate their combined or comparative effects across cellular aging panels within our comprehensive peptide catalog.
For gene expression assays, microarray analysis, and quantitative PCR (qPCR) studies, compound purity is paramount. Trace organic impurities, TFA salts, or endotoxins can confound gene expression data by triggering non-specific inflammatory signaling pathways in cultured cell lines. Consequently, sourcing high-purity materials is vital when seeking to buy Vilon gene expression reagents.
PX1 Research ensures every batch of Vilon exceeds 98.0% purity as confirmed by analytical RP-HPLC. Mass spectrometry verifies molecular weight (275.3 g/mol), while Limulus Amebocyte Lysate (LAL) testing certifies low endotoxin levels (<0.01 EU/mg). Every shipment includes a lot-specific, downloadable Certificate of Analysis (COA) generated by an independent, ISO 17025-accredited laboratory.
PX1 Research operates strictly within USA-based, GMP-compliant manufacturing parameters to guarantee chemical consistency and lot-to-lot reliability. Institutional buyers and academic laboratories requiring full supply chain transparency can access comprehensive documentation for compliance and internal audits.
Through our bulk institutional program, research organizations benefit from centralized lot tracking, specialized containerization, and expedited fulfillment. Orders are dispatched with same-day shipping (Monday–Friday) from our dual distribution hubs in California and Arizona, minimizing transit times and cold-chain disruption.
To preserve the structural integrity of lyophilized Vilon dipeptide during laboratory workflows, strict storage and reconstitution protocols must be followed:
1. **Lyophilized Powder Storage:** Store the sealed vial at -20°C for long-term stability. Avoid exposure to ambient moisture and light. 2. **Reconstitution:** Allow the vial to equilibrate to room temperature before reconstitution. Introduce sterile bacteriostatic water or laboratory-grade phosphate-buffered saline (PBS) down the inner glass wall. 3. **Dissolution:** Gently swirl the vial until the cake is completely dissolved. Avoid vigorous vortexing, which can introduce shear stress. 4. **Solution Storage:** Aliquot reconstituted solutions into single-use sterile cryovials and store at -20°C or -80°C to prevent freeze-thaw degradation.
What is the primary molecular sequence of Vilon?
Vilon is a synthetic dipeptide with the amino acid sequence L-lysyl-L-glutamic acid (Lys-Glu), with a chemical formula of C11H21N3O5 and a molecular weight of 275.3 g/mol.
How does Vilon modulate gene expression in preclinical research?
In vitro research indicates that Vilon binds directly to double-stranded DNA and histone proteins, inducing chromatin decondensation and allowing transcription factors increased access to previously silent genomic regions.
What analytical testing is conducted on PX1 Research Vilon?
Each lot undergoes RP-HPLC to verify chemical purity (>98%), mass spectrometry (ESI-MS) to confirm molecular weight, and LAL endotoxin testing at an independent ISO 17025 accredited laboratory.
What is the recommended solvent for reconstituting Vilon for in vitro assays?
For standard cellular assays, Vilon is commonly reconstituted using sterile bacteriostatic water, sterile water for injection, or sterile PBS, depending on specific cell culture media constraints.
How should reconstituted Vilon solutions be stored in the lab?
Reconstituted Vilon solutions should be aliquoted to avoid repeated freeze-thaw cycles and stored at -20°C or -80°C for extended experimental protocols.
How does Vilon differ from Thymalin or Thymosin Alpha-1?
Thymalin is a complex polypeptide extract and Thymosin Alpha-1 is a 28-amino-acid peptide. Vilon is a minimalist, synthetic two-amino-acid dipeptide (Lys-Glu) that targets specific epigenetic and thymic gene expression pathways.
Are PX1 Research compounds intended for human use?
No. All products sold by PX1 Research, including Vilon, are strictly intended for laboratory in vitro, preclinical, and scientific research use only. They are never for human or veterinary administration.
Where does PX1 Research ship orders from?
Orders ship directly from our USA distribution centers located in California and Arizona, featuring same-day fulfillment for orders placed Monday through Friday before cut-off times.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.