PX1 Research supplies reference-grade Cardiogen for sale, manufactured exclusively for in vitro and preclinical laboratory research. Every lot undergoes rigorous analytical testing—including RP-HPLC purity assessment, mass spectrometry identity confirmation, and endotoxin analysis—to deliver consistent reagents for cardiovascular and cell biology studies.
PX1 Research supplies reference-grade Cardiogen for sale, manufactured exclusively for in vitro and preclinical laboratory research. Every lot undergoes rigorous analytical testing—including RP-HPLC purity assessment, mass spectrometry identity confirmation, and endotoxin analysis—to deliver consistent reagents for cardiovascular and cell biology studies.
Cardiogen for sale through PX1 Research is produced specifically to meet the stringent standards of academic, biotechnology, and institutional laboratory investigators. As a synthetic tetrapeptide studied primarily within cardiovascular cell culture and tissue remodeling models, obtaining reference-standard material requires transparent analytical documentation and controlled domestic synthesis.
Researchers seeking to buy Cardiogen can access complete lot-specific documentation, including third-party Certificates of Analysis (COAs), High-Performance Liquid Chromatography (HPLC) chromatograms, and Electrospray Ionization Mass Spectrometry (ESI-MS) spectra. Explore our broader catalog of all peptides or consult our wholesale lab account portal for volume research inquiries.
Cardiogen is a short synthetic peptide comprising four amino acid residues: L-alanyl-L-glutamyl-L-aspartyl-L-arginine (Ala-Glu-Asp-Arg, sequence AEDA). Belonging to the class of short peptide bioregulators originally investigated for tissue-specific gene expression, its low molecular weight allows for rapid cellular uptake in cell culture systems.
Unlike complex recombinant proteins, short synthetic tetrapeptides present high chemical stability when properly lyophilized. In laboratory environments, Cardiogen is evaluated for its interaction with chromatin structural proteins and its capacity to alter transcriptional activity in mesenchymal and myocardial lineage cells without requiring complex carrier proteins.
Preclinical studies suggest that Cardiogen acts primarily by modulating gene expression related to cellular proliferation, extracellular matrix (ECM) turnover, and apoptotic pathways under metabolic or ischemic stress. In vitro assays using neonatal and adult ventricular myocytes demonstrate that exposure to Cardiogen influences p53 signalling, potentially attenuating stress-induced programmed cell death.
In cell culture models of cardiac fibroblasts, investigators have observed that Cardiogen application alters the ratio of collagen type I to collagen type III synthesis. By modulating matrix metalloproteinase (MMP) expression alongside tissue inhibitors of metalloproteinases (TIMPs), preclinical data indicate that the peptide plays a regulatory role in connective tissue reorganization following cellular hypoxia.
Scientific literature examining Cardiogen centers largely on preclinical rodent models of acute myocardial infarction and hypoxic stress. In rodent studies evaluating ischemia-reperfusion injury, administration of Cardiogen was associated with reduced necrotic lesion area and altered inflammatory cytokine expression, including lowered levels of TNF-alpha and IL-6 in surrounding tissue homogenates.
In vitro assays focusing on senescent human fibroblast lines have further evaluated Cardiogen's potential impact on cellular aging markers. Data derived from our peptide research library highlight how short peptide sequences, including Cardiogen, interact with histones to uncoil dense heterochromatin, thereby reactivating silenced genomic loci involved in cellular repair and protein synthesis.
Cardiogen belongs to an established family of ultra-short peptide bioregulators, but exhibits specific physiological targets when compared to related laboratory compounds. While Epitalon (Ala-Glu-Asp-Gly) is predominantly investigated for pineal gland regulation, telomerase activation, and broad systemic anti-aging mechanisms, Cardiogen (Ala-Glu-Asp-Arg) demonstrates specific tropism toward myocardial cell lines and vascular endothelium in preclinical literature.
Similarly, while tissue repair compounds such as BPC-157 act via angiogenic pathways (VEGFR2 activation) and gut-brain axis stabilization, and Thymogen operates as an immunomodulatory dipeptide (Glu-Trp), Cardiogen’s primary target profile remains focused on cardiac fibroblast differentiation, ECM homeostasis, and cardiomyocyte survival under hypoxic insult.
To ensure reproducible experimental outcomes, research peptides must maintain strict chemical purity standards free from truncated peptide fragments, residual coupling reagents, or heavy metal contamination. PX1 Research subjects every batch of Cardiogen to rigorous analytical verification performed by independent ISO 17025-accredited testing facilities.
Purity is quantitatively confirmed via Reverse-Phase HPLC (RP-HPLC), ensuring a baseline chemical purity of equal to or greater than 99%. Identity is verified using ESI-MS to confirm precise molecular mass (C18H31N7O9, MW: 489.48 g/mol). Furthermore, because bacterial endotoxins can invalidate cell culture assays by activating Toll-like receptors, PX1 Research measures endotoxin levels via Chromogenic LAL assays, certifying levels well below standard laboratory thresholds (<0.01 EU/mg).
Cardiogen is supplied as a sterile, lyophilized white powder sealed under inert gas. For optimal stability, unopened vials should be stored at -20°C or -80°C in a dry environment away from light. Under these cold storage conditions, the dry peptide remains stable for up to 24 months.
When preparing Cardiogen for in vitro assays, reconstitution should be performed using Bacteriostatic Water, Sterile Water for Injection, or sterile Phosphate-Buffered Saline (PBS, pH 7.4), depending on assay sensitivity. After solvent addition, gently swirl the vial until complete dissolution is achieved; vigorous vortexing should be avoided to prevent peptide shear. Reconstituted liquid solutions should be aliquoted into single-use polypropylene tubes and stored at -20°C to prevent degradation from repeated freeze-thaw cycles.
PX1 Research serves as a trusted domestic supplier of reference-standard compounds for biomedical laboratories, universities, and private research institutions across North America. All synthetic peptides are manufactured in compliance with cGMP-aligned synthesis protocols within US-based laboratories.
Orders ship directly from our centralized fulfillment centers in California and Arizona, utilizing fast, temperature-monitored shipping options with same-day dispatch for orders placed before cutoff times. Every order includes direct access to lot-matched COAs, ensuring total transparency, full chain-of-custody tracking, and analytical confidence for your research team.
What is Cardiogen used for in laboratory research?
Cardiogen (Ala-Glu-Asp-Arg) is evaluated in preclinical laboratory research to study cardiomyocyte survival, fibroblast proliferation, extracellular matrix remodeling, and cellular gene expression under hypoxic conditions.
What is the purity level of Cardiogen for sale at PX1 Research?
PX1 Research guarantees a chemical purity of ≥99% for Cardiogen, as verified by independent third-party RP-HPLC and ESI-MS testing.
How is Cardiogen tested for endotoxins?
Every lot of Cardiogen undergoes kinetic chromogenic Limulus Amebocyte Lysate (LAL) testing at an ISO 17025-accredited laboratory to ensure endotoxin levels remain below stringent research limits (<0.01 EU/mg).
What solvent should be used to reconstitute Cardiogen for in vitro assays?
Cardiogen is readily soluble in aqueous solutions. Depending on the research model, standard laboratory solvents include sterile Phosphate-Buffered Saline (PBS), Bacteriostatic Water, or Sterile Water for Injection.
How should lyophilized Cardiogen be stored upon arrival?
Lyophilized Cardiogen should be stored at -20°C or -80°C in a desiccated container. Reconstituted solutions should be aliquoted and frozen to minimize degradation from repeated freeze-thaw cycles.
What is the molecular weight and sequence of Cardiogen?
Cardiogen has the amino acid sequence Ala-Glu-Asp-Arg (AEDA) with a molecular formula of C18H31N7O9 and a molecular weight of 489.48 g/mol.
How does Cardiogen differ structurally from Epitalon?
While both are synthetic tetrapeptide bioregulators, Cardiogen features an Arginine residue at the C-terminus (Ala-Glu-Asp-Arg), targeting myocardial cell models, whereas Epitalon features a Glycine residue (Ala-Glu-Asp-Gly), targeting pineal and telomerase pathways.
Where does PX1 Research ship Cardiogen from?
All PX1 Research compounds ship directly from our USA facilities located in California and Arizona, offering fast domestic delivery and same-day dispatch Monday through Friday.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.