When sourcing Cardiogen for sale for qualified laboratory applications, researchers require verified chemical purity, precise sequence confirmation, and lot-specific analytical documentation. PX1 Research supplies high-purity, research-grade Cardiogen (Ala-Glu-Asp-Arg) manufactured in ISO 17025 and GMP-compliant facilities. Every lot undergoes independent third-party RP-HPLC and mass spectrometry testing to ensure sequence integrity and uncompromised quality for in vitro and preclinical research.
When sourcing Cardiogen for sale for qualified laboratory applications, researchers require verified chemical purity, precise sequence confirmation, and lot-specific analytical documentation. PX1 Research supplies high-purity, research-grade Cardiogen (Ala-Glu-Asp-Arg) manufactured in ISO 17025 and GMP-compliant facilities. Every lot undergoes independent third-party RP-HPLC and mass spectrometry testing to ensure sequence integrity and uncompromised quality for in vitro and preclinical research.
Investigating short-chain peptide bioregulators requires raw materials that adhere to rigorous analytical standards. When evaluating options for Cardiogen for sale, laboratory buyers must prioritize structural verification, purity guarantees, and batch consistency over unverified commercial claims. PX1 Research addresses these requirements by subjecting every lot of synthetic peptide to comprehensive analytical evaluation before release.
Researchers procuring reagents from our all peptides catalog receive documented proof of sequence integrity, precise purity percentages, and low endotoxin thresholds. To maintain full transparency across all study phases, our laboratory testing standards incorporate the following parameters:
1. Purity Verification: Every batch of Cardiogen is tested via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) to verify a minimum purity threshold of 98%. 2. Mass Spectrometry (MS): Electrospray ionization mass spectrometry (ESI-MS) confirms the exact molecular weight (490.47 g/mol) and primary sequence structure. 3. Endotoxin Control: Limulus Amebocyte Lysate (LAL) testing ensures endotoxin levels remain below 0.01 EU/mg, minimizing confounding variables in cell culture assays. 4. Analytical Certificate of Analysis (COA): Lot-specific COAs detailing raw HPLC and MS spectra are accessible for every container shipped. 5. Domestic Manufacturing & Fulfillment: Produced under GMP-compliant environments in the United States, with same-day dispatch from centralized distribution centers in California and Arizona.
Cardiogen is a synthetic tetrapeptide consisting of the amino acid sequence L-alanyl-L-glutamyl-L-aspartyl-L-arginine (Ala-Glu-Asp-Arg, or AEDA). Belonging to the class of short peptide bioregulators originally conceptualized in peptide gerontology, it is designed to mimic targeted endogenous signaling sequences within cardiovascular connective tissues.
The molecular formula of Cardiogen is C18H31N7O9, presenting a monoisotopic mass of 490.22 g/mol and a nominal molecular weight of 490.47 g/mol. Due to its hydrophilic side chains—specifically the carboxylic acid groups of glutamate and aspartate alongside the basic guanidino group of arginine—the peptide exhibits high solubility in aqueous buffer systems commonly utilized in preclinical laboratory protocols.
In structural assays, the short peptide chain demonstrates a lack of complex secondary folding, allowing direct interactions with nuclear chromatin and cell surface microdomains in vitro. Preclinical studies suggest that short peptide bioregulators like Cardiogen permeate nuclear membranes readily, interacting with specific histone proteins and DNA promoter sequences to modulate gene expression pathways.
In vitro and animal models have evaluated Cardiogen primarily for its role in modulating fibroblast activity, extracellular matrix dynamics, and cellular proliferation rates under simulated ischemic or oxidative stress conditions. Unlike broad-spectrum growth factors, short bioregulatory peptides demonstrate highly selective cellular target profiles.
Primary cardiac fibroblast cell cultures treated with Cardiogen exhibit altered expression profiles of key structural proteins. Preclinical studies suggest that exposure to the tetrapeptide influences the balance between type I and type III collagen synthesis, suppressing excessive fibrotic deposition while supporting physiological tissue remodeling. Furthermore, in vitro assays suggest Cardiogen downregulates pro-apoptotic signaling molecules—such as Bax—while maintaining expression of anti-apoptotic markers like Bcl-2 during hypoxic exposure.
In experimental rodent models of myocardial injury, administration of peptide bioregulators has been observed to influence scar tissue formation and microvascular density within damaged zones. Researchers investigating these mechanisms can explore related compounds within our cardiovascular research section to design robust comparative experimental frameworks.
To establish rigorous control parameters, laboratory protocols frequently compare Cardiogen against other well-studied tissue repair and bioregulatory compounds. While Cardiogen focuses specifically on cardiac fibroblast cellular dynamics, distinct peptides target broader tissue repair cascades or systemic chromatin remodeling.
For instance, researchers evaluating cell migration and vascular organization often compare Cardiogen to TB-500 (Thymosin Beta-4 fragment), which acts primarily via actin monomer sequestration and endothelial cell migration pathways. Similarly, BPC-157 is frequently utilized in comparative studies assessing nitric oxide synthase (NOS) modulation and focal adhesion kinase activation across diverse organ tissues.
When evaluating short peptide bioregulators specifically, Cardiogen shares structural and functional paradigms with Vilon (Lys-Glu) and Epitalon (Ala-Glu-Asp-Gly). While Epitalon targets telomerase expression and pineal gland activity in senescence models, Cardiogen remains focused on mesenchymal cell lines and cardiac tissue cultures. Cross-referencing these compounds within the PX1 Research library allows investigators to isolate target-specific gene regulation mechanisms from generalized cytoprotective responses.
Cardiogen is supplied as a lyophilized (freeze-dried) powder to maintain maximum molecular stability during transit and storage. Reconstitution must be performed under sterile laboratory conditions using appropriate aseptic techniques within a laminar flow hood.
For most cell culture and biochemical assays, sterile Bacteriostatic Water (0.9% benzyl alcohol) or sterile Phosphate-Buffered Saline (PBS, pH 7.4) serves as an ideal diluent. Standard laboratory reconstitution steps include:
1. Equilibrate the vial of lyophilized Cardiogen to room temperature for 20–30 minutes prior to reconstitution to prevent moisture condensation upon opening. 2. Clean the rubber stopper with a 70% isopropyl alcohol swab and allow it to air dry. 3. Using a calibrated micropipette or sterile syringe, slowly introduce the calculated volume of diluent along the inner glass wall of the vial. 4. Swirl the vial gently in a circular motion until the lyophilized cake is fully dissolved. Do not vortex vigorously, as physical agitation can disrupt peptide bonds or cause foaming. 5. Aliquot the reconstituted solution into single-use microcentrifuge tubes to prevent repeated freeze-thaw cycles prior to assay execution.
Maintaining chemical stability is essential for reproducing consistent experimental outcomes across multiple test series. Lyophilized Cardiogen must be stored in a climate-controlled freezer upon arrival to preserve structural integrity.
Unreconstituted lyophilized powder remains stable at -20°C for up to 24 months, or at -80°C for extended archival storage. Exposure to room temperature during standard shipping does not degrade the synthetic peptide, provided it is transferred to cold storage upon receipt. Avoid exposing the dry powder to direct light, excessive humidity, or temperatures exceeding 25°C for prolonged periods.
Once reconstituted in sterile aqueous buffer, liquid aliquots should be maintained at 2°C to 8°C if intended for immediate use within 48 to 72 hours. For longer-term storage of reconstituted solutions, freeze aliquots at -20°C or -80°C. Avoid repeated freeze-thaw cycles, which can cause aggregation or structural cleavage of the tetrapeptide chain.
The presence of bacterial endotoxins (lipopolysaccharides) in peptide reagents can severely compromise cell culture viability and induce artifactual inflammatory responses in preclinical models. PX1 Research prioritizes rigorous quality control to ensure all research compounds meet stringent analytical benchmarks.
Every batch of Cardiogen offered for sale undergoes automated kinetic chromogenic LAL assays to measure endotoxin content. Batches exceeding stringent analytical thresholds are rejected immediately. Furthermore, purity is verified via high-resolution RP-HPLC utilizing acetonitrile/water gradients containing 0.1% trifluoroacetic acid (TFA), ensuring accurate detection of trace synthesis byproducts or truncated sequences.
Researchers seeking to audit lot analytics before ordering can view analytical documentation or reach out to our team regarding custom requirements via our wholesale laboratory portal.
PX1 Research supports university laboratories, biotechnology enterprises, and contract research organizations (CROs) requiring reliable supplies of high-purity research compounds. Our domestic manufacturing setup ensures seamless scale-up from single-vial analytical screening to multi-gram bulk synthesis.
All shipments originate from our centralized distribution facilities in California and Arizona. Orders placed Monday through Friday before our daily cutoff time are dispatched same-day via expedited courier services, ensuring temperature stability and minimal transit times.
Principal investigators and laboratory managers seeking specialized formulation sizing, custom synthesis parameters, or recurring institutional fulfillment can establish dedicated wholesale accounts to access streamlined procurement services and lot reservation options.
What analytical standards confirm the quality of Cardiogen for sale at PX1 Research?
Every lot of Cardiogen undergoes independent third-party RP-HPLC to confirm >98% chemical purity and mass spectrometry (ESI-MS) to verify molecular weight (490.47 g/mol). Additionally, LAL assay testing ensures endotoxin levels remain below 0.01 EU/mg.
What is the precise chemical structure of Cardiogen?
Cardiogen is a synthetic tetrapeptide with the amino acid sequence Ala-Glu-Asp-Arg (L-alanyl-L-glutamyl-L-aspartyl-L-arginine). Its chemical formula is C18H31N7O9.
How does Cardiogen compare to BPC-157 or TB-500 in preclinical models?
While TB-500 acts on actin sequestration and BPC-157 modulates nitric oxide pathways broadly across tissues, Cardiogen is a short bioregulator targeting specific nuclear gene expression and extracellular matrix balance in cardiac fibroblast and cell cultures.
What solvent is recommended for reconstituting Cardiogen in laboratory assays?
Cardiogen dissolves readily in sterile bacteriostatic water or sterile Phosphate-Buffered Saline (PBS, pH 7.4). Solvents should be introduced gently along the glass wall without vortexing.
What are the recommended storage temperatures for lyophilized Cardiogen?
Lyophilized Cardiogen should be stored at -20°C for medium-term stability or -80°C for long-term archival storage. Reconstituted solution aliquots should be kept frozen to prevent degradation.
Where is PX1 Research Cardiogen manufactured and shipped from?
All PX1 Research compounds are manufactured in domestic, GMP-compliant facilities in the United States and dispatched directly from fulfillment centers in California and Arizona.
Is a Certificate of Analysis (COA) included with Cardiogen orders?
Yes. Every shipment of Cardiogen includes access to lot-specific third-party COAs featuring raw HPLC chromatograms and mass spectra.
What is the endotoxin limit for PX1 Research Cardiogen?
PX1 Research enforces a strict endotoxin threshold of less than 0.01 EU/mg, verified via LAL testing to prevent unwanted inflammatory artifacts in cell assays.
Can institutions purchase Cardiogen in bulk for large-scale studies?
Yes. Qualified academic and commercial institutions can open wholesale laboratory accounts for volume pricing, custom batch sizing, and dedicated lot reservations.
Is Cardiogen intended for human consumption or clinical administration?
No. Cardiogen is supplied strictly as a research-grade chemical reagent for in vitro laboratory evaluation and preclinical animal studies. It is not for human or veterinary use.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.