PX1 Research provides laboratory-grade Epitalon (Epithalon) tetrapeptide for qualified academic, biotechnology, and institutional researchers. Synthesized under strict quality controls in USA-based facilities, every lot undergoes rigorous analytical verification to guarantee maximum stability, purity, and experimental reproducibility.
PX1 Research provides laboratory-grade Epitalon (Epithalon) tetrapeptide for qualified academic, biotechnology, and institutional researchers. Synthesized under strict quality controls in USA-based facilities, every lot undergoes rigorous analytical verification to guarantee maximum stability, purity, and experimental reproducibility.
Qualified laboratories seeking to source high-purity epitalon peptide for sale can obtain analytical-grade reagents through PX1 Research. Epitalon (Ala-Glu-Asp-Gly) is supplied as a lyophilized powder with guaranteed chromatographic purity exceeding 99%. Every batch is manufactured in USA-based GMP-compliant facilities and accompanied by an ISO 17025 third-party Certificate of Analysis (COA) detailing RP-HPLC analysis, mass spectrometry verification, and endotoxin testing.
Epitalon is intended strictly for in vitro, cellular, and animal model research applications. Institutional buyers and primary investigators can explore our complete catalog of research peptides or register for a specialized wholesale research account to support large-scale longitudinal studies.
Epitalon (also known as Epithalon or Epithalone) is a synthetic tetrapeptide consisting of the amino acid sequence L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine (H-Ala-Glu-Asp-Gly-OH). It was originally developed as a synthetic analogue of epithalamin, a natural peptide extract derived from the pineal gland.
Structurally, Epitalon belongs to the short-chain peptide bioregulator family. Bioregulators are short amino acid sequences that interact directly with chromatin structure to modulate gene expression without altering the underlying genomic code. Preclinical data suggest that short peptides like Epitalon can penetrate cell membranes and nuclear envelopes to bind specific promoter regions of DNA, initiating targeted transcriptional cascades.
Because of its low molecular weight (390.35 g/mol), Epitalon exhibits rapid aqueous solubility and favorable biochemical stability compared to larger protein complexes. Researchers interested in molecular biology mechanisms can review detailed biochemical documentation in our centralized research hub.
The primary focus of Epitalon research centers on its capacity to activate telomerase, the ribonucleoprotein reverse transcriptase enzyme responsible for maintaining chromosomal integrity. In somatic cells, telomeres progressively shorten with each cycle of DNA replication, eventually triggering replicative senescence or apoptosis once a critical threshold is reached.
In vitro assays using human somatic fibroblast cultures indicate that exposure to Epitalon induces the expression of the human telomerase reverse transcriptase (hTERT) catalytic subunit. By up-regulating hTERT activity, Epitalon facilitates the elongation of telomeric repeats at the 3' ends of chromosomes, effectively delaying the entry of cultured cells into the Hayflick limit.
Furthermore, animal models evaluating age-associated genomic instability demonstrate that Epitalon administration reduces chromatic aberrations and maintains nuclear architecture. These findings suggest that Epitalon plays a pivotal role in epigenetic regulation, chromatin decondensation, and the preservation of genomic stability during extended cell passaging.
Beyond its direct action on telomeric DNA, Epitalon is extensively studied for its interactions with the neuroendocrine system, specifically regarding pineal gland function and diurnal rhythm regulation. Preclinical rodent models demonstrate that pineal synthetic activity declines significantly with age, leading to disrupted melatonin synthesis and altered circadian gene profiles.
Experimental data indicate that Epitalon application restores nocturnal melatonin secretion patterns in aging non-human primates and rodent models. By modulating the hypothalamic-pituitary-epiphyseal axis, Epitalon helps maintain normal diurnal rhythmicity in experimental subjects subjected to altered photoperiods or physiological stress.
In vitro pinealocyte culture models further demonstrate that Epitalon upregulates the activity of key enzymes involved in melatonin biosynthesis, such as serotonin N-acetyltransferase (AANAT). This dual action—combining telomeric preservation with endocrine recalibration—makes Epitalon a unique candidate for systems-level research in cellular physiology.
Over three decades of preclinical investigation have documented the effects of Epitalon across various experimental paradigms. In long-term rodent studies, chronic administration of Epitalon resulted in a statistically significant reduction in spontaneous tumor incidence, alongside marked improvements in antioxidant enzyme activity, such as superoxide dismutase (SOD) and glutathione peroxidase (GPx).
Key preclinical findings documented in peer-reviewed literature include:
• Extension of functional cell lifespan in cultured human fetal fibroblast models without malignant transformation. • Suppression of spontaneous carcinogenesis and inhibition of mammary tumor development in female mice models. • Restoration of immune response markers, including T-cell differentiation parameters, in senescent animal models. • Reduction in lipid peroxidation markers and systemic oxidative stress indicators in experimental subjects.
Researchers evaluating cellular senescence markers often analyze epitalon 10mg alongside secondary markers of mitochondrial function and stress response pathways to assess compound efficacy.
When sourcing an epitalon peptide for sale, rigorous analytical verification is paramount to ensure reproducibility in laboratory settings. Impurities resulting from incomplete solid-phase peptide synthesis (SPPS)—such as deletion sequences or side-chain protecting group adducts—can distort experimental outcomes and introduce off-target variables.
PX1 Research subjects every lot of Epitalon to rigorous analytical testing in an ISO 17025 accredited laboratory:
1. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Determines peptide purity profiles. PX1 Research enforces a strict threshold where Epitalon must exhibit >99% chromatographic purity, with single peak detection at 214 nm or 220 nm. 2. Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms exact molecular weight (monoisotopic mass of 390.35 Da), ensuring absolute sequence identity and the absence of truncation side-products. 3. Water Content & Elemental Analysis: Verifies correct moisture levels post-lyophilization to ensure stable mass measurements when preparing working concentrations.
Bacterial endotoxins (lipopolysaccharides) present a severe risk in cell culture and preclinical animal assays. Even trace quantities of endotoxin can trigger inflammatory responses, activation of Toll-like receptor 4 (TLR4), and downstream gene expression changes that compromise research integrity.
PX1 Research verifies that all Epitalon lots meet stringent endotoxin thresholds using the Limulus Amebocyte Lysate (LAL) chromogenic assay compliant with USP <85> standards. Our research-grade peptides are guaranteed to contain < 0.05 EU/mg of endotoxin. Additionally, sterile filtration (0.22 µm) and lyophilization protocols are conducted within ISO Class 5 cleanrooms to ensure minimal bioburden and prevent bacterial degradation during handling.
To preserve the structural stability of lyophilized Epitalon, proper laboratory reconstitution and handling techniques must be observed. The lyophilized cake should be stored at -20°C or -80°C for long-term preservation, protected from light and moisture.
Reconstitution Protocol for Bench Science:
• Equilibrate the vial to room temperature prior to reconstitution to minimize moisture condensation upon opening. • Reconstitute using sterile target solvents such as Bacteriostatic Water, Sterile Water for Injection, or Phosphate-Buffered Saline (PBS, pH 7.4), depending on the requirements of the downstream assay. • Gently swirl the vial to facilitate solubilization; avoid vigorous vortexing or mechanical agitation, which can induce physical shear stress and peptide denaturation. • Prepare single-use working aliquots and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles, as cyclic phase transitions degrade peptide integrity over time.
Epitalon is part of a broader class of short-chain peptide bioregulators studied for their tissue-specific gene expression effects. Understanding the distinction between these compounds is essential when designing comparative preclinical trials.
While Epitalon acts primarily on pineal function and telomerase expression, Pinealon (Glu-Asp-Arg) is a tripeptide specifically targeted toward central nervous system and neuronal cell survival models. Similarly, Thymalin focuses on immune modulation and T-cell maturation pathways, whereas tissue-remodeling studies frequently utilize GHK-Cu for extracellular matrix regulation. Combining or contrasting these short peptides allows researchers to evaluate pathway-specific responses in multi-factorial longevity protocols.
PX1 Research is dedicated to providing the scientific community with reliable, high-purity research compounds. When purchasing an epitalon peptide for sale through our platform, institutions receive complete transparency and verified quality across every batch.
Our Commitment to Quality Standards:
• 100% USA Synthesized and Packaged: Direct oversight of production parameters ensures consistency. • Lot-Specific COAs: Independent, third-party HPLC and MS analytical reports accompany every shipment. • Rapid Logistics: Orders are dispatched same-day (Monday through Friday) from our centralized distribution hubs in California and Arizona. • Secure Procurement: Dedicated support for institutional purchase orders and academic research accounts.
All materials sold by PX1 Research are strictly intended for in vitro assays, laboratory experiments, and non-clinical preclinical research. Explore our catalog or contact our analytical support team to source verified reagents for your ongoing projects.
What is the primary target mechanism of Epitalon in cell culture models?
In preclinical cellular models, Epitalon primarily targets the upregulation of human telomerase reverse transcriptase (hTERT) expression, which promotes telomerase enzyme activity and maintains telomeric length during cell division.
What purity level is guaranteed for Epitalon peptide at PX1 Research?
PX1 Research guarantees a minimum of 99% purity for Epitalon, as verified by independent Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Mass Spectrometry (MS) testing.
How is Epitalon shipped and packaged for stability?
Epitalon is supplied as a lyophilized (freeze-dried) powder sealed in amber glass vials under vacuum. It is shipped from our CA and AZ facilities with same-day dispatch M-F to preserve chemical stability during transit.
What solvent should be used to reconstitute Epitalon for laboratory assays?
Epitalon is readily soluble in sterile water, bacteriostatic water, or laboratory-grade phosphate-buffered saline (PBS, pH 7.4). Solvents should be selected based on the specific requirements of the planned in vitro or animal model protocol.
What is the endotoxin threshold for PX1 Research Epitalon?
Every lot of Epitalon undergoes Limulus Amebocyte Lysate (LAL) testing compliant with USP <85> standards, ensuring endotoxin levels remain strictly below 0.05 EU/mg to prevent confounding inflammatory signals in research.
How should reconstituted Epitalon stock solutions be stored?
Once reconstituted, working aliquots should be stored at -20°C or -80°C to prevent degradation. Repeated freeze-thaw cycles should be avoided to maintain molecular integrity.
Is Epitalon approved for human consumption or therapeutic use?
No. Epitalon supplied by PX1 Research is strictly for laboratory research, in vitro experiments, and preclinical animal studies. It is not for human or veterinary use, therapy, or clinical administration.
Does PX1 Research offer batch-specific Certificates of Analysis (COA)?
Yes. Every shipment of Epitalon includes access to a lot-specific COA containing full RP-HPLC spectra, ESI-MS mass verification, and endotoxin assay results from an ISO 17025 accredited laboratory.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.