Epitalon (Epithalon) is a synthetic tetrapeptide bioregulator composed of L-alanyl-L-glutamyl-L-aspartyl-glycine (Ala-Glu-Asp-Gly). Developed to mimic the endogenous pineal peptide epithalamin, it is extensively studied in preclinical models for its role in telomerase activation, telomere length maintenance, and pineal-circadian axis regulation. PX1 Research supplies high-purity, USA-manufactured Epitalon exclusively for in vitro and preclinical laboratory research.
Epitalon (Epithalon) is a synthetic tetrapeptide bioregulator composed of L-alanyl-L-glutamyl-L-aspartyl-glycine (Ala-Glu-Asp-Gly). Developed to mimic the endogenous pineal peptide epithalamin, it is extensively studied in preclinical models for its role in telomerase activation, telomere length maintenance, and pineal-circadian axis regulation. PX1 Research supplies high-purity, USA-manufactured Epitalon exclusively for in vitro and preclinical laboratory research.
Qualified institutional and independent researchers seeking to buy Epitalon peptide for sale in the USA require analytical precision, guaranteed sequence integrity, and strict lot-to-lot consistency. Epitalon is a synthetic short-chain bioregulating peptide designed to replicate the biological activity of pineal gland extracts. In controlled laboratory environments, it serves as a primary tool for examining genomic stability, cellular senescence, and enzymatic activity involved in chromosomal preservation.
PX1 Research manufactures Epitalon within domestic, ISO 17025-accredited and GMP-compliant facilities in California and Arizona. Every lot undergoes rigorous quality control, including high-performance liquid chromatography (HPLC) to confirm purity levels exceeding 99%, electrospray ionization mass spectrometry (ESI-MS) to confirm exact molecular weight, and chromogenic LAL assays to ensure minimal endotoxin burden. All orders ship same-day from domestic distribution hubs to preserve peptide stability during transit.
Epitalon (CAS 307297-39-8) is a synthetic tetrapeptide with the primary amino acid sequence H-Ala-Glu-Asp-Gly-OH and a molecular weight of 390.35 g/mol. Categorized biochemically as a peptide bioregulator, its short sequence allows it to interact directly with chromatin structure within cell nuclei. Unlike large globular proteins or complex signaling factors, short bioregulatory peptides exhibit distinct binding dynamics that allow them to penetrate nuclear membranes and interact with specific histone and DNA motifs.
In cell-free and cell-culture models, bioregulators like Epitalon demonstrate site-specific interaction with promoter regions of genes regulating cellular lifespan and enzymatic synthesis. Researchers studying our comprehensive all peptides catalog frequently utilize Epitalon alongside other low-molecular-weight synthetic cascades to map fundamental gene expression patterns across prolonged culture passages.
The principal biological mechanism attributed to Epitalon in published literature centers on the transcriptional activation of the telomerase reverse transcriptase (TERT) gene. Telomerase is the ribonucleoprotein enzyme complex responsible for synthesizing telomeric repeat sequences (TTAGGG) at the terminal ends of eukaryotic chromosomes. Telomere shortening occurs progressively with each round of somatic cell division, serving as a primary marker of replicative senescence.
In vitro assays utilizing human somatic cell lines, such as fetal fibroblasts and retinal pigment epithelial cells, indicate that exposure to Epitalon induces TERT expression. This upregulation correlates with measurable telomerase enzymatic activity, leading to telomere elongation and an extended Hayflick limit in cultured populations. Preclinical data suggest that Epitalon may induce localized chromatin decondensation, enhancing accessibility for transcription factors at the promoter region of the TERT gene without altering primary genomic sequence structure.
Beyond its direct action on chromosomal structures, Epitalon is heavily investigated for its neuroendocrine and circadian effects in animal models. The pineal gland plays a central role in orchestrating biological rhythms through the cyclical synthesis of melatonin from tryptophan. Aging rodent and non-human primate models exhibit a progressive blunting of peak nocturnal melatonin secretion, which downstream impacts metabolic homeostasis and immune surveillance.
Preclinical administration of Epitalon in aged rodent models has been shown to restore nocturnal melatonin synthesis to levels observed in younger control cohorts. This restoration appears to occur via upregulation of key enzymes in the melatonin biosynthetic pathway, such as arylalkylamine N-acetyltransferase (AANAT). Investigating these neuroendocrine pathways provides critical data on how central circadian pacemakers influence systemic biological aging markers.
When designing experimental protocols focused on cellular longevity and metabolic function, researchers often compare Epitalon with other targeted research compounds. While Epitalon functions primarily as a nuclear bioregulator targeting telomerase expression and genomic architecture, mitochondrial-targeted peptides operate through distinct metabolic and bioenergetic pathways.
For instance, SS-31 targets the inner mitochondrial membrane where it binds selectively to cardiolipin, preventing electron leakage and reducing reactive oxygen species (ROS) production under oxidative stress conditions. Conversely, MOTS-c is a mitochondrial-derived peptide that translocates to the nucleus under metabolic stress to regulate folate cycle kinetics and glucose utilization. Epitalon differs fundamentally from these agents by acting directly on nuclear chromatin accessibility and TERT gene expression rather than regulating acute bioenergetic flux. Researchers exploring these complementary mechanisms can reference our guide on SS-31 mitochondrial research for comparative baseline methodologies.
Establishing consistent experimental conditions is vital for obtaining reproducible data when investigating synthetic bioregulators. In vitro research using Epitalon typically involves treating primary cell cultures across serial passages to measure changes in doubling capacity, senescence-associated beta-galactosidase (SA-β-gal) activity, and baseline telomere length using Quantitative Fluorescence In Situ Hybridization (Q-FISH) or quantitative PCR (qPCR).
Typical cell culture media formulations incorporate Epitalon at concentrations ranging from 0.1 μM to 10 μM, added during routine media changes. Because Epitalon lacks complex tertiary structure, its solution stability in standard cell culture incubators (37°C, 5% CO2) is stable over standard 24- to 48-hour incubation intervals. Detailed protocol design considerations and assay cross-validations are routinely updated in the PX1 research hub.
When purchasing raw materials for laboratory research, vendor verification is the most critical step in safeguarding data integrity. Analytical transparency ensures that observed cellular responses are attributable solely to the target sequence and not to residual synthesis reagents, truncated peptide fragments, or bacterial contaminants.
PX1 Research enforces strict analytical standards for every lot of Epitalon 10mg. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing:
1. Reverse-Phase HPLC (RP-HPLC): Establishes chemical purity by measuring peak area integration at 214 nm, ensuring target purity exceeds 99.0% with minimal synthesis side-products. 2. Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms the exact mass-to-charge ratio (m/z) corresponding to the target theoretical monoisotopic mass of 390.35 Da. 3. Endotoxin Testing: Measures lipopolysaccharide (LPS) levels using kinetic chromogenic LAL assays to ensure levels remain below strictly defined threshold limits (<0.01 EU/mg).
Bacterial endotoxins represent a severe confounding variable in biological research. Even minute concentrations of endotoxin can trigger inflammatory signaling cascades via Toll-like receptor 4 (TLR4) activation in cell lines, skewing gene expression data, cytokine profiling, and apoptosis assays.
To prevent experimental artifacts, PX1 Research implements sterile manufacturing processes under ISO-certified cleanroom conditions. Reconstitution of lyophilized Epitalon should always be performed inside a certified Class II Biosafety Cabinet using sterile, endotoxin-free solvents such as molecular biology grade water or sterile phosphate-buffered saline (PBS, pH 7.4). Institutional accounts requiring large-scale allocations can access our specialized wholesale lab portal for batch-matched material lots.
Epitalon is supplied as a lyophilized (freeze-dried) sterile powder to ensure maximal chemical stability during storage and transport. Due to its hydrophilic amino acid composition (containing Aspartic Acid and Glutamic Acid residues), Epitalon demonstrates exceptional aqueous solubility in standard laboratory buffers.
Recommended storage and handling procedures include:
• Long-Term Storage (Lyophilized): Store at -20°C or -80°C in a manual defrost freezer protected from light and moisture. Sealed vials remain stable for up to 24 months under these conditions. • Reconstitution: Allow the vial to equilibrate to room temperature prior to reconstitution to prevent moisture condensation inside the stopper. Reconstitute using sterile bacteriostatic water or PBS. • Aliquoting and Storage (Reconstituted): Once dissolved, prepare single-use working aliquots in sterile microcentrifuge tubes to avoid repeated freeze-thaw cycles. Store working aliquots at -20°C for short-term experimental series (up to 3 months) or -80°C for extended storage.
Procuring reliable peptides for scientific investigation requires a transparent, domestic supply chain. Importing unverified materials from overseas manufacturers introduces risks of degradation from ambient heat exposure during prolonged transit, customs delays, and inconsistent purity verification.
PX1 Research mitigates these risks by maintaining full domestic synthesis, packaging, and testing operations within the USA. Operating out of facilities in California and Arizona, PX1 guarantees same-day shipping on all qualified research orders placed Monday through Friday before 3:00 PM PST. Researchers gain immediate access to lot-matched COAs, responsive technical support, and uncompromised chemical quality designed specifically for rigorous laboratory investigation.
What is Epitalon and what is its primary role in research?
Epitalon (Ala-Glu-Asp-Gly) is a synthetic tetrapeptide bioregulator designed to mimic the endogenous pineal peptide epithalamin. In preclinical research, it is studied for its ability to upregulate telomerase reverse transcriptase (TERT) gene expression, maintain telomere length, and modulate circadian neuroendocrine signaling.
How does PX1 Research verify the purity of Epitalon?
Every lot of Epitalon supplied by PX1 Research undergoes independent third-party testing utilizing Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) to confirm purity (>99%) and Electrospray Ionization Mass Spectrometry (ESI-MS) to confirm exact molecular weight.
What endotoxin controls are applied to PX1 Epitalon lots?
PX1 Research performs kinetic chromogenic Limulus Amebocyte Lysate (LAL) testing on all peptide batches to ensure endotoxin levels are maintained below strictly defined laboratory standards (<0.01 EU/mg), preventing non-specific immune activation in cell culture assays.
Is Epitalon suitable for human consumption or therapeutic administration?
No. Epitalon provided by PX1 Research is strictly sold as a research compound intended exclusively for in vitro laboratory experimentation and preclinical scientific evaluation. It is not for human, veterinary, or clinical use.
What is the recommended reconstitution solvent for Epitalon in vitro assays?
Epitalon easily dissolves in sterile molecular biology grade water or sterile phosphate-buffered saline (PBS, pH 7.4). For assays requiring extended fluid storage, bacteriostatic water containing 0.9% benzyl alcohol may be utilized depending on cellular toxicity parameters.
How should reconstituted Epitalon stock solutions be stored?
Reconstituted Epitalon should be divided into single-use aliquots to prevent repeated freeze-thaw cycles and stored at -20°C or -80°C. Aliquots stored at -20°C remain stable for up to 3 months, while lyophilized vials stored at -20°C remain stable for up to 24 months.
How does Epitalon compare to mitochondrial research peptides like SS-31 or MOTS-c?
Epitalon acts primarily within the cell nucleus to influence chromatin structure and TERT gene transcription. In contrast, SS-31 targets mitochondrial inner membrane cardiolipin to mitigate ROS production, and MOTS-c modulates systemic bioenergetics and metabolic signaling cascades.
Where does PX1 Research manufacture and ship its peptides?
PX1 Research manufactures and packages its synthetic peptides in state-of-the-art, GMP-compliant facilities located in California and Arizona. Orders ship domestically same-day Monday through Friday.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.