PX1 Research supplies USA-manufactured, high-purity Epithalon 20mg for qualified laboratory researchers evaluating synthetic bioregulator peptides. Each batch undergoes rigorous third-party analytical testing, including RP-HPLC and mass spectrometry, to guarantee >99% peptide purity and verified low endotoxin levels for reliable in vitro and preclinical experimental design.
PX1 Research supplies USA-manufactured, high-purity Epithalon 20mg for qualified laboratory researchers evaluating synthetic bioregulator peptides. Each batch undergoes rigorous third-party analytical testing, including RP-HPLC and mass spectrometry, to guarantee >99% peptide purity and verified low endotoxin levels for reliable in vitro and preclinical experimental design.
Qualified institutions and academic researchers seeking Epithalon 20mg for sale require verified analytical purity, batch consistency, and reliable domestic sourcing. Epithalon (also known as Epitalon or Ala-Glu-Asp-Gly) is a synthetic pineal tetrapeptide studied extensively in cellular aging and neuroendocrine models. Acquiring research-grade Epithalon requires verifying that the material has undergone rigorous third-party testing rather than relying on unverified international bulk suppliers.
When procuring Epithalon 20mg from PX1 Research, principal investigators receive lyophilized peptide vials manufactured under strict quality standards in the United States. Every lot is backed by a downloadable Certificate of Analysis (COA) detailing identity via mass spectrometry (MS), purity via reverse-phase high-performance liquid chromatography (RP-HPLC), and quantitative endotoxin testing. This analytical transparency ensures experimental reproducibility across longitudinal research protocols.
Epithalon is a synthetic short-chain peptide comprising four amino acid residues: L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine ($C_{14}H_{22}N_4O_9$, MW: 390.35 g/mol). Originally conceptualized to mimic the bioactive properties of Epithalamin—a crude pineal gland extract isolated by Soviet researchers—Epithalon was designed as a targeted bioregulator peptide capable of modulating gene expression and cellular longevity pathways.
As a short peptide (tetrapeptide), Epithalon possesses distinct physicochemical properties compared to larger polypeptide hormones. Its low molecular weight allows high bio-accessibility in cellular cultures and animal tissue assays. In our expansive research peptide catalog, Epithalon remains one of the primary candidates for investigating chromating architecture, chromatin condensation changes, and peptide-DNA interaction mechanisms in aging cells.
The primary biochemical pathway associated with Epithalon in published literature centers on telomerase activation and telomere length regulation. In vitro assays using human somatic cells demonstrate that Epithalon induces the catalytic subunit of telomerase (human telomerase reverse transcriptase, or hTERT). By promoting hTERT gene expression, Epithalon facilitates the enzymatic elongation of telomeric repeats at the ends of chromosomes, effectively bypassing the Hayflick limit in specific cultured cell lines.
Beyond direct telomerase activation, preclinical studies suggest that Epithalon interacts directly with nuclear DNA. Structural bio-computational models and cell-free binding assays reveal that the Ala-Glu-Asp-Gly sequence binds selectively to specific major and minor grooves of DNA, inducing localized de-condensation of heterochromatin. This chromatin remodeling mechanism permits reactivation of silent genes associated with cellular repair, antioxidant enzyme synthesis (such as superoxide dismutase), and ribosomal RNA synthesis in aging tissues. For further reading on pineal peptide signaling, visit our peptide research library.
In addition to telomere dynamics, Epithalon plays a major role in neuroendocrine and circadian rhythm research. The pineal gland exhibits a progressive decline in melatonin secretion during physiological aging. Preclinical rodent models and non-human primate studies demonstrate that administration of Epithalon restores baseline nocturnal melatonin synthesis and normalizes circadian secretion profiles without suppressing endogenous gland function.
Data from aged animal models show that Epithalon restores the sensitivity of the hypothalamus to hormonal feedback loops, modulating the hypothalamic-pituitary-adrenal (HPA) axis. By regulating melatonin secretion pathways, researchers observe improvements in sleep-wake cycle architecture, reduced oxidative stress markers in CNS tissue, and restored gonadotropic responsiveness in aged laboratory specimens.
When designing comparative longevity or cellular aging studies, investigators often evaluate Epithalon alongside other short-chain peptide bioregulators and metabolic regulators. Understanding the distinct signaling cascades of each compound is essential for structuring multi-arm experimental protocols.
While Epithalon specifically targets pineal function, hTERT expression, and telomere maintenance, Pinealon 20mg is a tripeptide (Glu-Asp-Arg) engineered specifically for selective neuroprotective and cognitive bio-regulation in central nervous system cell lines. Similarly, Thymalin acts primarily within the immune axis, stimulating T-cell differentiation and thymic epithelial signaling. For metabolic and mitochondrial longevity research, researchers frequently contrast Epithalon's nuclear DNA interaction with MOTS-c 10mg, a mitochondria-derived peptide that targets AMPK activation and metabolic homeostasis rather than nuclear telomerase induction.
Because synthetic short peptides can suffer from sequence deletions, truncation artifacts, or residual TFA (trifluoroacetic acid) contamination during solid-phase peptide synthesis (SPPS), rigorous laboratory verification is imperative. PX1 Research enforces stringent quality control measures across all product batches.
Every batch of Epithalon 20mg undergoes dual analytical verification in an ISO 17025-accredited laboratory. Reverse-Phase HPLC establishes chromatographic purity (consistently exceeding 99.0%), ensuring no truncated peptide impurities remain. Electrospray Ionization Mass Spectrometry (ESI-MS) confirms exact molecular mass matching the target sequence. Furthermore, chromogenic LAL assays verify that endotoxin content remains strictly under <0.5 EU/mg, preventing unspecific inflammatory responses in sensitive cell culture or tissue assays.
Epithalon 20mg is supplied as a sterile, lyophilized white cake or powder inside a sealed, glass vial with a flip-off cap. To preserve peptide integrity and prevent premature enzymatic or hydrolytic degradation, strict handling protocols must be observed by research personnel. Review our complete peptide reconstitution guide for detailed laboratory procedures.
Prior to reconstitution, store lyophilized Epithalon vials at -20°C in a desiccated environment, shielded from light. Reconstitute using sterile bacteriostatic water or sterile normal saline (0.9% NaCl) depending on your assay requirements. Gently swirl the vial until the lyophilized cake fully dissolves; avoid vigorous mechanical vortexing to prevent peptide shearing. Once reconstituted, liquid aliquots should be stored at 2°C to 8°C for short-term assays (up to 28 days) or flash-frozen in single-use aliquots at -80°C for long-term storage.
PX1 Research provides fast, reliable distribution of research-grade peptides directly to university laboratories, biotech institutes, and contract research organizations (CROs). All orders ship directly from domestic fulfillment hubs located in California and Arizona, guaranteeing rapid transit times and avoiding international customs delays.
Orders placed Monday through Friday before 12:00 PM PST ship same-day via expedited courier services with temperature-monitored packaging option upon request. For academic labs or corporate entities requiring high-volume supplies, visit our wholesale peptide portal to apply for verified institutional purchasing accounts and tier-structured pricing.
What is the molecular sequence of Epithalon?
Epithalon is a synthetic tetrapeptide with the amino acid sequence L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine (Ala-Glu-Asp-Gly).
What is the primary application of Epithalon 20mg in laboratory research?
Epithalon 20mg is utilized in preclinical research to investigate telomerase activation (hTERT expression), telomere extension, chromatin de-condensation, melatonin regulation, and pineal gland bio-regulation.
How is Epithalon purity verified by PX1 Research?
Every lot of Epithalon 20mg undergoes third-party verification using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) for purity (>99%) and Mass Spectrometry (MS) for exact molecular weight verification.
What is the endotoxin threshold for PX1 Research Epithalon 20mg?
All batches are tested using a chromogenic LAL assay to guarantee endotoxin levels remain below 0.5 EU/mg, ensuring suitability for delicate cellular models.
How should lyophilized Epithalon 20mg be stored upon arrival?
Unreconstituted lyophilized vials should be stored at -20°C (or -80°C for long-term stability) in a dry, dark environment to prevent thermal degradation.
What diluent is recommended for reconstituting Epithalon 20mg?
Sterile bacteriostatic water (0.9% benzyl alcohol) or sterile laboratory-grade 0.9% sodium chloride saline is recommended depending on the specific in vitro or preclinical protocol.
Does PX1 Research ship Epithalon internationally?
PX1 Research primary fulfillment occurs domestically within the United States from hubs in California and Arizona, providing same-day shipping M-F for verified domestic research institutions.
Is Epithalon approved for human clinical use or administration?
No. Epithalon provided by PX1 Research is strictly designated for in vitro, biochemical, and preclinical laboratory research use only. It is not intended for human or veterinary medical use, diagnosis, or treatment.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.