Gonadorelin acetate (CAS 33515-09-2) is a synthetic decapeptide identical in amino acid sequence to natural hypothalamic gonadotropin-releasing hormone (GnRH). PX1 Research supplies high-purity (≥99%) Gonadorelin exclusively for laboratory evaluation of pituitary receptor dynamics, gonadotropin secretion assays, and HPG axis signal transduction.
Gonadorelin acetate (CAS 33515-09-2) is a synthetic decapeptide identical in amino acid sequence to natural hypothalamic gonadotropin-releasing hormone (GnRH). PX1 Research supplies high-purity (≥99%) Gonadorelin exclusively for laboratory evaluation of pituitary receptor dynamics, gonadotropin secretion assays, and HPG axis signal transduction.
Gonadorelin (CAS 33515-09-2) is a synthetic hypothalamic decapeptide identical to endogenous gonadotropin-releasing hormone (GnRH). In preclinical laboratory models, it binds to G-protein coupled GnRH receptors on pituitary gonadotropes to stimulate pulsatile luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release. Research-grade Gonadorelin at ≥99% purity is intended exclusively for in vitro signaling assays and animal endocrine studies, not for human consumption or anabolic administration.
When evaluating compound databases using broad terms like gonadorelin body building peptides hormones 99% purity cas 33515-09-2, investigators must distinguish between theoretical physiological pathways discussed in literature and validated analytical standards. High-purity peptides are required in cell culture and preclinical models to prevent bioassay interference caused by truncated peptide sequences, organic solvent residues, or bacterial endotoxins.
Gonadorelin is chemically designated as 5-oxo-L-prolyl-L-histidyl-L-tryptophyl-L-seryl-L-tyrosyl-glycyl-L-leucyl-L-arginyl-L-prolylglycinamide, represented by the CAS registry number 33515-09-2. The chemical structure consists of ten amino acid residues: PyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2, featuring a cyclized N-terminal pyroglutamyl residue and a C-terminal glycinamide modification. These terminal modifications protect the peptide against rapid exopeptidase degradation in cell culture media and biochemical buffers.
The theoretical molecular weight of Gonadorelin free base is 1182.3 g/mol, typically supplied as an acetate salt formulation to optimize aqueous solubility and freeze-drying stability. Investigators seeking precise bioassay baseline controls can review our certified high-purity Gonadorelin acetate product for complete physical specification sheets.
In physiological and laboratory models, endogenous GnRH is released from hypothalamic neurons in a pulsatile manner, traversing the hypophyseal portal system to act upon gonadotrope cells in the anterior pituitary gland. Gonadorelin activates the high-affinity GnRH receptor (GnRH-R), a seven-transmembrane G-protein coupled receptor linked to the Gαq/11 signaling cascade.
Binding triggers intracellular phospholipase C (PLC) activation, generating inositol trisphosphate (IP3) and diacylglycerol (DAG). This pathway mobilizes intracellular calcium stores and activates protein kinase C (PKC), subsequently stimulating transcription and exocytosis of both LH and FSH. Researchers studying endogenous GnRH receptor signaling mechanisms utilize Gonadorelin to measure downstream phosphorylation of extracellular signal-regulated kinases (ERK1/2) and mitogen-activated protein kinases (MAPK).
Search queries incorporating terms like 'body building peptides hormones' often stem from open-literature discussions surrounding muscle physiology, androgen pathways, and recovery mechanisms in cellular models. However, Gonadorelin itself possesses no direct anabolic or androgen receptor binding activity. Its primary biological function in research settings is strictly mediated through anterior pituitary gonadotropin release.
In scientific literature, researchers evaluate how transient spikes in LH and FSH secondary to GnRH agonist administration influence downstream steroidogenesis in isolated Leydig or granulosa cell cultures. PX1 Research maintains a strict laboratory-only policy: all materials listed across our research peptide catalog are synthesized for analytical testing, assay calibration, and in vitro investigation, strictly prohibited from human therapeutic or athletic performance use.
Preclinical trials in rodent and non-human primate models have extensively characterized the dose-dependent response of pituitary gonadotropes to exogenous Gonadorelin challenge. In vitro assays using primary anterior pituitary cell cultures demonstrate that continuous, constant exposure to Gonadorelin results in GnRH-R desensitization and receptor downregulation within 24 to 48 hours. Conversely, intermittent, pulsatile delivery preserves receptor sensitivity and maintains sustained LH/FSH secretion profiles.
Furthermore, animal models evaluating HPG axis recovery following exogenous hormone suppression utilize Gonadorelin to measure the functional reserve capacity of pituitary gonadotropes. Researchers contrast these dynamics with upstream endocrine regulators by exploring Kisspeptin-10 research assays, which stimulate endogenous hypothalamic GnRH release rather than acting directly on pituitary receptors.
Within hypothalamic-pituitary research, investigators frequently compare short-acting natural sequence peptides with long-acting synthetic analogs. While Gonadorelin (CAS 33515-09-2) exhibits a rapid biological half-life of 2 to 10 minutes due to swift cleavage by neutral endopeptidases, modified analogs such as Triptorelin research grade peptide contain D-amino acid substitutions (e.g., D-Trp at position 6) that dramatically increase enzymatic stability and receptor binding affinity. While natural Gonadorelin requires pulsatile dosing in cell perfusion models to avoid desensitization, extended-release agonists are selected when sustained receptor blockade and chemical castration models are required.
Additionally, laboratories evaluating broader neuroendocrine pathways often contrast HPG axis modulators with growth hormone secretagogues like CJC-1295 without DAC or tissue repair signaling peptides such as BPC-157 research standards. Each compound class target distinct receptor families, requiring specific handling protocols and assay conditions detailed in our analytical laboratory hub.
For accurate laboratory experimentation, peptide purity must be thoroughly verified. Impurities such as deletion sequences, incomplete cleavage fragments, or residual trifluoroacetic acid (TFA) salts can distort binding kinetic data and introduce cellular toxicity in bioassays. PX1 Research subjects every synthesis lot to rigorous quality control procedures:
1. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Ensures chemical purity meets or exceeds 99.0%, resolving trace impurities and side-products. 2. Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms exact monoisotopic mass and structural identity corresponding to CAS 33515-09-2. 3. Chromogenic LAL Endotoxin Testing: Guarantees endotoxin levels are maintained below <0.01 EU/mg, protecting sensitive primary cell cultures from inflammatory artifact interference. Every shipment includes a lot-specific Certificate of Analysis (COA) directly traceable to our ISO 17025 accredited testing facilities.
To maintain the structural integrity of lyophilized Gonadorelin acetate in laboratory settings, strict storage and handling protocols must be observed:
- Lyophilized Storage: Store the dry powder at -20°C to -80°C in a desiccated environment away from direct light. Under these conditions, the peptide remains stable for up to 24 months. - Reconstitution: Reconstitute using sterile bacteriostatic water or laboratory-grade phosphate-buffered saline (PBS, pH 7.4). Allow the vial to equilibrate to room temperature before adding solvent to prevent condensation inside the container. - Solubilization: Gently swirl or invert the vial. Avoid aggressive vortexing or vigorous shaking, which can cause surface denaturation and peptide aggregation. - Aliquoting: Once reconstituted, divide the solution into single-use working aliquots and store at -80°C to prevent degradation from freeze-thaw cycles. Reconstituted aqueous solutions should be used within 30 days.
PX1 Research is dedicated to supporting scientific advancement by supplying American-manufactured research peptides manufactured under strict cGMP guidelines. We recognize that reproducible experimental outcomes depend on absolute chemical purity, consistent lot-to-lot homogeneity, and robust supply chain integrity.
Institutions and independent investigators seeking bulk quantities or ongoing contract supply can explore our wholesale lab account programs. Orders placed Monday through Friday ship same-day directly from our centralized distribution facilities in California and Arizona, complete with comprehensive documentation and verified HPLC/MS analytical reports.
What is Gonadorelin (CAS 33515-09-2) used for in research?
Gonadorelin (CAS 33515-09-2) is used in endocrine research to evaluate pituitary gonadotrope function, GnRH receptor binding kinetics, and the pulsatile regulation of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in cell culture and preclinical animal models.
Why is 99% purity essential for Gonadorelin in laboratory assays?
High purity (≥99%) ensures that observed cellular responses are driven solely by the Gonadorelin molecule rather than truncated peptide fragments, synthesis byproducts, or endotoxins that could alter receptor binding curves or cause cell death.
How does Gonadorelin differ from long-acting GnRH agonists like Triptorelin?
Gonadorelin possesses the natural sequence of endogenous GnRH with a short biological half-life (minutes), whereas synthetic agonists like Triptorelin contain structural modifications that prolong receptor occupancy, leading to rapid receptor desensitization.
How is the purity of PX1 Research Gonadorelin verified?
Every lot of PX1 Research Gonadorelin undergoes Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) for purity determination and Electrospray Ionization Mass Spectrometry (ESI-MS) for identity confirmation in an ISO 17025 accredited laboratory.
What solvent should be used to reconstitute research-grade Gonadorelin?
Sterile bacteriostatic water or sterile phosphate-buffered saline (PBS, pH 7.4) is typically recommended for reconstituting lyophilized Gonadorelin in analytical and cell culture settings.
What are the endotoxin limits for PX1 Research peptides?
PX1 Research peptides undergo LAL chromogenic endotoxin testing to ensure levels remain strictly below <0.01 EU/mg, minimizing inflammatory background noise in in vitro assays.
Is Gonadorelin approved for human body building or athletic use?
No. Gonadorelin supplied by PX1 Research is strictly designated for laboratory research use only. It is not intended for human consumption, clinical treatment, body building, or athletic administration.
Where does PX1 Research manufacture and ship its peptides?
All PX1 Research compounds are manufactured in USA-based cGMP facilities and shipped same-day (M–F) from distribution hubs located in California and Arizona.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.