For accurate MT1 reconstitution in laboratory settings, dissolve lyophilized MT1 (Melanotan-1) powder using sterile bacteriostatic water under a laminar flow hood. PX1 Research supplies high-purity MT1 (10mg) manufactured in the USA, backed by third-party HPLC/MS and endotoxin COAs for every lot, with same-day shipping M–F from California and Arizona to support uninterrupted experimental workflows.
For accurate MT1 reconstitution in laboratory settings, dissolve lyophilized MT1 (Melanotan-1) powder using sterile bacteriostatic water under a laminar flow hood. PX1 Research supplies high-purity MT1 (10mg) manufactured in the USA, backed by third-party HPLC/MS and endotoxin COAs for every lot, with same-day shipping M–F from California and Arizona to support uninterrupted experimental workflows.
Reconstituting Melanotan-1 (MT1) requires introducing a precise volume of sterile diluent—typically Bacteriostatic Water containing 0.9% benzyl alcohol—into the vacuum-sealed vial containing lyophilized MT1 peptide. Gently direct the diluent along the glass wall rather than directly onto the lyophilized cake to prevent agitation and structural shear of the peptide chain.
For a standard 10mg vial of MT1, adding 2.0 mL of diluent yields a working concentration of 5.0 mg/mL (or 500 mcg per 0.1 mL). If a more dilute solution is required for precise micro-pipetting in cell culture or binding assays, adding 5.0 mL of diluent results in a concentration of 2.0 mg/mL (200 mcg per 0.1 mL). Swirl the vial gently until completely dissolved; never shake.
Once dissolved, reconstituted MT1 must be maintained at 2°C to 8°C for short-term benchwork or aliquot-frozen at -20°C to -80°C for long-term study. To maintain experimental reproducibility, always source verified reagents from our catalog of research peptides.
Melanotan-1 (MT1), also referred to in scientific literature as Afamelanotide or [Ac-Ser-Tyr-Ser-Met-Glu-His-D-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2], is a synthetic peptide analog of alpha-melanocyte-stimulating hormone (α-MSH). In preclinical models, MT1 acts as a non-selective agonist of melanocortin receptors, displaying high affinity for the melanocortin-1 receptor (MC1R).
Primary research pathways focus on evaluating MT1 for melanocortin activity related to skin pigmentation responses, UV-induced cellular protection mechanisms, and receptor-binding kinetics. Unlike its cyclic derivative Melanotan-2, MT1 maintains a linear peptide structure that closely mirrors endogenous α-MSH while exhibiting increased biological stability against enzymatic degradation.
Researchers investigating melanocortin receptor signaling or comparative binding affinity can review analytical profiles across compounds such as Melanotan-2 or review detailed compound documentation in the PX1 Research library.
Executing a clean and precise MT1 reconstitution requires aseptic technique and appropriate laboratory consumables to avoid contamination or loss of compound integrity:
- High-Purity MT1: A verified vial of MT1 Melanotan-1 10mg. - Diluent: Bacteriostatic Water (0.9% benzyl alcohol) for multi-dose laboratory assays, or Sterile Water for Injection (SWFI) for single-use assays sensitive to alcohol preservatives. - Alcohol Prep Swabs: 70% Isopropyl alcohol wipes for sanitizing rubber stoppers and vial tops. - Reconstitution Syringe: Sterile 1mL to 3mL luer-lock syringe fitted with a 21G to 25G needle. - Micro-Pipettes and Tips: Calibrated pipettors for precise volumetric measurement during assay preparation. - Cryovials: Sterile polypropylene tubes if preparing aliquots for low-temperature storage.
Achieving consistent liquid concentrations during mt1 reconstitution demands strict adherence to volumetric protocols. Below is the standard laboratory procedure for reconstituting a standard 10mg vial:
1. Preparation and Sanitization: Wipe down the biosafety cabinet working surface. Remove the flip-off protective cap from the 10mg MT1 vial and sanitize the exposed rubber septum with a 70% isopropyl swab. Allow to air dry for 30 seconds.
2. Drawing the Diluent: Draw the designated volume of Bacteriostatic Water into a sterile syringe. For a 5 mg/mL target concentration, draw exactly 2.0 mL of diluent.
3. Equalizing Pressure: Insert the needle into the diluent container, draw the liquid, and prior to puncturing the peptide vial, draw an equal volume of air if necessary. However, because PX1 Research vials are sealed under a partial vacuum, the vacuum will naturally draw the diluent into the vial.
4. Slow Reconstitution: Place the needle through the center of the rubber stopper at a 45-degree angle pointing toward the glass wall. Allow the vacuum to slowly draw the liquid down the inner wall. Do not inject liquid directly onto the lyophilized powder.
5. Complete Dissolution: Once diluent is fully introduced, equalize any remaining pressure by withdrawing an equivalent volume of air. Gently swirl the vial in circular movements. Dissolution typically occurs within 30 to 60 seconds. Do not shake vigorously to avoid foaming and denaturing peptide chains.
To streamline calculations when determining how to reconstitute mt1 10mg vials, researchers can refer to the concentration mapping below based on the volume of Bacteriostatic Water added to a standard 10mg lyophilized sample:
- 1.0 mL Diluent Added: Concentration = 10.0 mg/mL (1,000 mcg per 0.1 mL / 100 µL) - 2.0 mL Diluent Added: Concentration = 5.0 mg/mL (500 mcg per 0.1 mL / 100 µL) - 2.5 mL Diluent Added: Concentration = 4.0 mg/mL (400 mcg per 0.1 mL / 100 µL) - 5.0 mL Diluent Added: Concentration = 2.0 mg/mL (200 mcg per 0.1 mL / 100 µL)
Selecting the appropriate dilution depends on the sensitivity of your analytical instruments and micro-pipetting tolerances. For high-precision binding or cell viability assays, higher dilution volumes (e.g., 2.5 mL or 5.0 mL) reduce volumetric margin of error.
Lyophilized MT1 powder is stable at room temperature for brief transit periods, but long-term preservation requires cold storage at -20°C. Upon receiving your shipment from PX1 Research, place un-reconstituted vials immediately into freezer storage protected from light.
Once reconstituted, Bacteriostatic Water preserves the peptide against microbial growth at 2°C to 8°C for up to 28 days. If using Sterile Water without preservatives, reconstituted MT1 must be used immediately or aliquot-frozen to prevent biological contamination.
Avoid repeated freeze-thaw cycles, which induce structural degradation of the peptide sequence. Divide reconstituted MT1 into single-use cryo-aliquots prior to deep freezing at -20°C or -80°C. For institutional procurement requirements, explore our wholesale supply program.
Selecting a reliable research peptide vendor requires validating objective quality metrics rather than marketing claims. When evaluating suppliers for critical assay materials, verify the following standards:
- Purity Verification: Every lot must undergo High-Performance Liquid Chromatography (RP-HPLC) achieving ≥99% purity. - Identity Confirmation: Mass Spectrometry (MS) verification matching theoretical molecular weight (1646.85 g/mol). - Endotoxin Testing: Quantitative LAL assay verification confirming endotoxin levels remain below strict laboratory limits (<0.1 EU/mg). - Sourcing & Manufacturing: USA-based synthesis and rigorous cold-chain logistics. - Lot Traceability: Individual lot numbers linked directly to downloadable, third-party analytical Certificates of Analysis (COAs). - Dispatch & Delivery: Rapid fulfillment from regional hubs (CA + AZ) with same-day dispatch M–F.
Substandard peptides introduce uncontrolled variables that compromise experimental validity. Lab managers should avoid vendors displaying any of the following commercial red flags:
1. Generic or Opaque COAs: Certificate of Analysis documents that lack batch numbers, show edited spectral graphs, or fail to name an independent third-party analytical lab. 2. Foreign Drop-Shipping: Suppliers shipping directly from overseas without domestic quality control testing, exposing sensitive proteins to prolonged heat transit. 3. Lack of Endotoxin Data: Vendors who measure HPLC purity but omit bacterial endotoxin testing, risking contaminated cell culture environments. 4. Non-Specific Powder Appearance: Lyophilized cakes that appear discolored, melted, or collapsed prior to reconstitution, indicating excess residual solvent or moisture compromise.
PX1 Research provides verified, domestic-grade reagents to academic institutions, biotechnology firms, and independent research facilities across North America. Every shipment of our MT1 Melanotan-1 10mg includes vacuum-sealed, rubber-stoppered glass vials engineered for optimal reconstitution consistency.
Orders placed before 12:00 PM PST ship same-day M–F directly from our California and Arizona fulfillment centers via tracked domestic ground or expedited air delivery. Each lot includes instant digital access to its corresponding HPLC, MS, and endotoxin COA records.
To source certified peptides for your next preclinical research study, view our complete inventory at the PX1 Research catalog.
mt1 reconstitution: what diluent should be used?
For multi-dose laboratory testing, reconstituted MT1 should be prepared using sterile Bacteriostatic Water containing 0.9% benzyl alcohol. The benzyl alcohol inhibits bacterial contamination during repeated needle insertions over a 28-day window. If benzyl alcohol interferes with sensitive cell culture systems, sterile unpreserved 0.9% sodium chloride or sterile water for injection may be substituted for immediate single-use assays.
how to reconstitute mt1 10mg vials?
To reconstitute a 10mg MT1 vial, sanitize the rubber stopper with 70% isopropyl alcohol. Inject 2.0 mL of Bacteriostatic Water slowly along the inside glass wall of the vial using a sterile needle. Allow the liquid to absorb into the cake without shaking, then gently swirl until fully dissolved, resulting in a 5.0 mg/mL concentration.
What ratio of bacteriostatic water should be added to 10mg MT1?
The ratio depends on your target concentration. Adding 1.0 mL of bacteriostatic water yields 10 mg/mL (1000 mcg/0.1 mL), adding 2.0 mL yields 5 mg/mL (500 mcg/0.1 mL), and adding 5.0 mL yields 2 mg/mL (200 mcg/0.1 mL). A 2.0 mL diluent addition is standard for standard laboratory pipetting.
How long is reconstituted MT1 stable in cold storage?
When reconstituted with Bacteriostatic Water, MT1 maintains chemical stability for up to 28 days when kept refrigerated between 2°C and 8°C (36°F to 46°F). For extended research timelines beyond 30 days, reconstituted solution should be divided into single-use aliquots and stored at -20°C or -80°C.
Is MT1 legal to purchase for research in the US?
Yes, MT1 (Melanotan-1) is fully legal to purchase and possess in the United States for laboratory, educational, and preclinical research purposes. It is strictly not approved for human consumption, personal use, or therapeutic application.
Do you provide a COA for my specific MT1 lot?
Yes, PX1 Research includes a lot-specific Certificate of Analysis (COA) with every order. Each COA provides third-party verified RP-HPLC purity curves, mass spectrometry mass verification, and LAL endotoxin testing results corresponding to your exact lot number.
What is the difference between MT1 and MT2 in laboratory research?
MT1 (Melanotan-1) is a linear peptide analog of alpha-MSH that selectively target MC1R receptors involved in skin pigmentation studies. MT2 (Melanotan-2) is a truncated cyclic analog that binds across multiple melanocortin receptor subtypes (MC1R, MC3R, MC4R, MC5R), demonstrating different receptor activation profiles in preclinical models.
How fast does PX1 Research ship MT1 orders?
PX1 Research dispatches orders same-day Monday through Friday for purchases submitted before 12:00 PM PST. Shipments originate directly from our fulfillment centers in California and Arizona via tracked domestic shipping.
What purity level is guaranteed for PX1 MT1 10mg?
PX1 Research guarantees a minimum purity level of ≥99% for all MT1 10mg batches, as verified by independent reverse-phase high-performance liquid chromatography (RP-HPLC).
Can MT1 be reconstituted with sterile water instead of BAC water?
Yes, MT1 can be reconstituted with sterile water for injection (SWFI). However, because sterile water lacks a preservative, the reconstituted solution must be used immediately in single-use assays or frozen into single-use aliquots to prevent bacterial degradation.
How should lyophilized MT1 powder be stored prior to reconstitution?
Unopened, lyophilized MT1 powder should be stored in a freezer at -20°C to maintain structural integrity long-term. Keep the vial sealed away from light and excessive humidity prior to initial reconstitution.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.