Melanotan 2 60mg — Vial Spec, Reconstitution Math & COA
The melanotan 2 60mg vial specification is engineered for high-throughput laboratory environments requiring large-volume master stocks or extended parallel assay runs. This guide provides exact reconstitution calculations, volumetric aliquot planning, and analytical quality benchmarks for researchers evaluating synthetic melanocortin analogs in preclinical settings.
Quick answer
The melanotan 2 60mg vial specification is engineered for high-throughput laboratory environments requiring large-volume master stocks or extended parallel assay runs. This guide provides exact reconstitution calculations, volumetric aliquot planning, and analytical quality benchmarks for researchers evaluating synthetic melanocortin analogs in preclinical settings.
Key takeaways
- In high-throughput cell culture models, receptor binding screens, and automated assay workflows, sourcing research compounds in expanded mass quantities minimizes lot-to-lot variance and reduces setup latency.
- When planning experimental protocols, researchers must evaluate whether a single high-mass vial or multiple lower-mass vials best suit their workflow.
- [Melanotan](/research-peptides/melanotan-2) II features the amino acid sequence Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-NH2, with a molecular formula of C50H69N15O9 and a characteristic monoisotopic mass of approximately 1024.18 Da.
- Reconstituting a 60mg cake of lyophilized peptide requires careful volumetric planning to ensure exact stock concentration without exceeding the saturation threshold or creating an overly viscous solution.
Introduction to the 60mg Melanotan 2 Lyophilized Specification
In high-throughput cell culture models, receptor binding screens, and automated assay workflows, sourcing research compounds in expanded mass quantities minimizes lot-to-lot variance and reduces setup latency. The melanotan 2 60mg presentation represents a high-yield lyophilized cake formulation designed specifically to satisfy large-scale preclinical project demands.
Melanotan II (MT-2) is a synthetic cyclic heptapeptide analog of the endogenous alpha-melanocyte-stimulating hormone (α-MSH). In preclinical research literature, this compound is primarily evaluated for its non-selective agonist activity across central and peripheral melanocortin receptors. Laboratories ordering a 60mg format typically conduct high-density microplate binding assays, extended pharmacokinetic rodent studies, or multi-week tissue culture experiments that require a single, highly uniform master stock solution.
PX1 Catalog Availability & Format Considerations
When planning experimental protocols, researchers must evaluate whether a single high-mass vial or multiple lower-mass vials best suit their workflow. While some industrial supply facilities distribute a consolidated 60mg format, PX1 Research primarily inventories individual, single-assay Melanotan 2 10mg vials as our standard reference stock. This standard size minimizes potential peptide degradation from repeated vial punctures, thermal cycling, and extended storage of reconstituted liquid stock.
For institutional laboratories requiring high-capacity orders equivalent to or exceeding 60mg total mass, PX1 provides streamlined multi-vial allocations through our wholesale lab account portal. Utilizing smaller individual vials allows research teams to reconstruct fresh working solutions per assay plate while maintaining identical lot consistency across our full line of all research peptides.
Molecular Architecture and Receptor Dynamics
Melanotan II features the amino acid sequence Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-NH2, with a molecular formula of C50H69N15O9 and a characteristic monoisotopic mass of approximately 1024.18 Da. The structural cyclization between the Asp and Lys side chains confers enhanced metabolic stability against enzymatic cleavage compared to native linear MSH peptides.
Preclinical binding studies indicate that Melanotan II functions as a potent, non-selective agonist at melanocortin receptor subtypes MC1R, MC3R, MC4R, and MC5R. In vitro assays demonstrate high affinity at MC1R and MC4R, where receptor activation initiates intracellular cyclic adenosine monophosphate (cAMP) signaling cascades. In laboratory models, these interactions are routinely investigated to map melanogenesis, dermatological pigmentation responses, and central nervous system melanocortin pathways.
Reconstitution Mathematics for 60mg Bulk Vials
Reconstituting a 60mg cake of lyophilized peptide requires careful volumetric planning to ensure exact stock concentration without exceeding the saturation threshold or creating an overly viscous solution. To calculate final working concentration ($C$), divide total mass ($M = 60\text{ mg} = 60,000\ \mu\text{g}$) by total diluent volume ($V$ in mL or $\mu\text{L}$): $C = M / V$.
When adding 1.0 mL of sterile diluent to a 60mg vial, the resulting concentration is $60\text{ mg/mL}$ ($60\ \mu\text{g/\mu L}$). At this high density, thorough dissolution requires gentle room-temperature equilibration and delicate axial rotation—never intense vortexing—to avoid foaming and peptide denaturation. If utilizing 2.0 mL of solvent, the yield is $30\text{ mg/mL}$ ($30\ \mu\text{g/\mu L}$). Reconstituting with 3.0 mL yields $20\text{ mg/mL}$ ($20\ \mu\text{g/\mu L}$). For automated precision pipetting, researchers can calculate custom micro-volume conversions using our interactive peptide reconstitution calculator.
Diluent Selection and Solubilization Standards
Selecting the appropriate solvent is critical when handling high-density peptide cakes. Bacteriostatic Water for Injection (containing 0.9% benzyl alcohol) is standard for multi-use research containers requiring microbial inhibition over multi-day assay windows. For short-term single-day cellular assays where benzyl alcohol could interfere with cell viability, sterile 0.9% Sodium Chloride (normal saline) or sterile water for injection (WFI) is preferred.
In instances where high mass concentration approaches physical solubility limits, adding a small volume of sterile phosphate-buffered saline (PBS, pH 7.4) can assist in maintaining physiological osmolarity for downstream application. In vitro data indicate that synthetic melanocortin analogs remain stable in neutral pH buffers when protected from direct atmospheric exposure.
Aliquot Planning and Storage Stability
A primary challenge with large-format vials like the melanotan 2 60mg specification is managing solution stability after initial reconstitution. Reconstituted peptides in aqueous solution are susceptible to hydrolysis, aggregation, and oxidation over time, particularly when subjected to repeated temperature fluctuations.
To mitigate degradation, primary stock solutions should be immediately aliquoted into single-use, low-binding polypropylene microcentrifuge tubes (e.g., 100 $\mu\text{L}$ to 500 $\mu\text{L}$ working volumes). Lyophilized powder should be stored at $-20^\circ\text{C}$ to $-80^\circ\text{C}$ in a desiccated environment. Reconstituted liquid aliquots should be flash-frozen and kept at $-80^\circ\text{C}$ for extended preservation, avoiding repeated freeze-thaw cycles that break peptide bonds.
Quality Verification: COA, HPLC, and Mass Spectrometry
High-yield research protocols depend on stringent analytical verification. Every raw batch processed for PX1 Research undergoes multi-point analytical screening at an independent ISO 17025 accredited laboratory prior to release. Every lot is verified for identity, purity, and safety metrics before entering stock.
Purity is measured via High-Performance Liquid Chromatography (HPLC), guaranteeing $\ge 99\%$ main-peak area purity free from truncated synthesis side-products. Sequence identity and molecular mass are confirmed using Electrospray Ionization Mass Spectrometry (ESI-MS). Furthermore, every lot undergoes chromogenic LAL testing to confirm endotoxin levels fall below strict safety thresholds ($<0.01\text{ EU/mg}$). Researchers can independently verify batch data by reviewing our published certificate of analysis hub.
Comparative Analysis: Related Melanocortin Compounds
When designing comparative signaling or binding affinity assays, researchers often benchmark Melanotan II against other synthesized peptides within the melanocortin family. Each analog possesses unique selectivity, binding kinetics, and structural modifications that alter receptor recruitment.
In comparative literature, Melanotan 1 (Afamelanotide) serves as a linear peptide analog that closely mimics native α-MSH with selective receptor binding, whereas MT-2 features a cyclic structure that significantly increases receptor binding duration and potency. Similarly, research examining the PT-141 research compound (Bremelanotide)—a hydroxylated metabolite derivative of MT-2—focuses heavily on selective central MC3R and MC4R pathways without significant primary interaction at peripheral MC1R sites. Exploring these structural variations inside our broad melanocortin research peptides index allows investigators to select the exact molecular candidate required for their hypothesis.
Assay Integration and Methodological Protocols
In vitro research protocols investigating melanogenesis frequently utilize murine B16-F10 melanoma cell lines or human primary melanocyte cultures. Melanotan II is introduced to the culture media in nanomolar concentrations ($1\text{ nM}$ to $100\text{ nM}$) to measure dose-dependent stimulation of tyrosinase activity and melanin synthesis.
In preclinical animal models, research studies evaluate systemic administration parameters to analyze central nervous system signaling, energy homeostasis, and peripheral skin pigmentation responses. Data derived from these experiments must strictly account for stock solution concentration accuracy, reinforcing the necessity for meticulous reconstitution math when handling high-yield options like the melanotan 2 60mg format.
Shipping, Logistics, and Supply Chain Integrity
Peptide integrity during transit is vital for reliable research outcomes. PX1 Research packs all lyophilized compounds in cold-insulated, temperature-controlled packaging designed to prevent thermal exposure during transport. Products ship same-day (Monday through Friday) directly from our centralized distribution facilities in California and Arizona.
To review technical documentation, material safety data sheets (MSDS), or to consult with our laboratory support specialists regarding bulk assay planning, explore our comprehensive peptide research hub. All products supplied by PX1 Research are strictly designated for laboratory research use only.
Frequently Asked Questions
What is the primary research focus for Melanotan 2 60mg?
Melanotan 2 is a synthetic melanocortin receptor agonist. Preclinical studies evaluate its activity at MC1R, MC3R, MC4R, and MC5R receptors in models investigating skin pigmentation responses, tyrosinase stimulation, and central melanocortin signaling.
How do I calculate the concentration of a 60mg vial reconstituted with 2mL of bacteriostatic water?
Dividing the total peptide mass (60mg or 60,000µg) by the diluent volume (2mL or 2,000µL) yields a final concentration of 30 mg/mL (30 µg/µL).
Does PX1 Research stock Melanotan 2 in a single 60mg vial?
PX1 primarily carries single-run Melanotan 2 10mg vials to preserve peptide stability and prevent degradation caused by repeated vial entry. Equivalent or larger total masses can be ordered in multi-vial sets through our wholesale portal.
How should a reconstituted 60mg stock solution be stored?
Reconstituted liquid stock should be divided into single-use micro-aliquots using low-binding microcentrifuge tubes and stored at -80°C to prevent freeze-thaw degradation and bacterial contamination.
What purity documentation is provided with PX1 peptides?
Every lot is accompanied by a lot-matched Certificate of Analysis (COA) from an independent ISO 17025 accredited laboratory, featuring HPLC purity curves (≥99%), ESI-MS mass verification, and endotoxin assay results.
What solvent is recommended for high-concentration peptide reconstitution?
Bacteriostatic Water (0.9% benzyl alcohol) is recommended for multi-use research containers. Sterile 0.9% saline or PBS may be used for sensitive cell culture assays where benzyl alcohol must be avoided.
What is the difference between Melanotan 1 and Melanotan 2 in laboratory research?
Melanotan 1 is a linear peptide analog that closely mimics endogenous α-MSH, whereas Melanotan 2 is a cyclic heptapeptide analog with enhanced metabolic stability and broader receptor affinity across MC1R–MC5R.
Are PX1 Research compounds intended for human use?
No. All products sold by PX1 Research are strictly for laboratory research use only in in vitro and preclinical animal models. They are not for human, clinical, or veterinary administration.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.