Access verified batch documentation and analytical testing standards for SNAP-8 (Acetyl Octapeptide-3). PX1 Research provides comprehensive, lot-specific Certificates of Analysis derived from independent ISO 17025 accredited laboratory testing to ensure optimal reproducibility in preclinical research.
Access verified batch documentation and analytical testing standards for SNAP-8 (Acetyl Octapeptide-3). PX1 Research provides comprehensive, lot-specific Certificates of Analysis derived from independent ISO 17025 accredited laboratory testing to ensure optimal reproducibility in preclinical research.
A SNAP-8 certificate of analysis (COA) is an official quality control document verifying the identity, chemical purity, and safety profile of Acetyl Octapeptide-3 for laboratory research use only. PX1 Research provides lot-specific COAs featuring independent ISO 17025 third-party test results, including Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) purity analysis demonstrating ≥98% purity, Electrospray Ionization Mass Spectrometry (ESI-MS) molecular mass verification, and quantitative LAL endotoxin testing.
To maintain rigorous scientific standards, every batch of synthesized peptide undergoes individual evaluation before release. Researchers evaluating biomimetic peptide signaling pathways can inspect detailed chromatograms and spectral output to confirm sequence integrity, lack of truncated peptide artifacts, and batch consistency.
SNAP-8 (Acetyl Octapeptide-3) is an elongated synthetic analogue of the N-terminal end of SNAP-25, designed to study substrate competition within the SNARE (Soluble NSF Attachment Protein Receptor) complex. Understanding the precise molecular architecture of the peptide is critical when analyzing its corresponding SNAP-8 certificate of analysis.
Chemical Formula: C41H70N16O16S1 Exact Molecular Mass: 1075.16 g/mol (Monoisotopic) Sequence: Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 Counterion: Trifluoroacetate (TFA) or Acetate (lot dependent) CAS Number: 868844-74-0 Physical State: Lyophilized white to off-white powder
In preclinical model systems, SNAP-8 is evaluated for its capacity to disrupt the assembly of the ternary SNARE complex, thereby inhibiting vesicle fusion and neurotransmitter release in vitro. Verifying the chemical sequence via mass spectrometry ensures that researchers are testing the exact 8-amino-acid structure rather than shorter degradation fragments or un-capped peptides.
Every batch of SNAP-8 supplied by PX1 Research undergoes rigorous testing across several parameters. The certificate of analysis explicitly outlines these analytical metrics, ensuring full transparency for institutional laboratories and procurement teams.
1. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Measures the relative area percentage of the target peak against synthetic impurities or truncated peptide sequences. PX1 Research requires an RP-HPLC purity threshold of ≥98.0%. 2. Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms the molecular weight of Acetyl Octapeptide-3. The observed m/z values must match the theoretical molecular weight of 1075.16 Da (accounting for ionization adducts such as [M+H]+ or [M+2H]2+). 3. Endotoxin Assay (LAL Test): Quantifies bacterial endotoxin contamination. Preclinical in vitro cell cultures require extremely low endotoxin exposure to prevent baseline inflammatory activation. 4. Appearance and Solubility Test: Verifies physical state homogeneity and complete dissolution in sterile aqueous buffers.
Researchers seeking detailed methodologies regarding analytical testing can review our guide on HPLC and mass spectrometry analysis.
Reading a certificate of analysis requires understanding how RP-HPLC peak integration and ESI-MS ion peaks reflect chemical purity. On a standard C18 analytical column run with a gradient of acetonitrile/water containing 0.1% TFA, SNAP-8 produces a distinct, sharp elution peak.
The primary elution peak represents pure Acetyl Octapeptide-3. Minor secondary peaks represent hydrophobic or hydrophilic synthesis byproducts, such as deleted amino acid sequences or un-deprotected side chains. PX1 Research calculates purity by integrating the area under the curve (AUC) of the principal peak relative to total integrated peak area. Chromatograms showing broad peaks, split peaks, or substantial baseline noise indicate compromised compound integrity.
Mass spectra complement HPLC by verifying identity. For SNAP-8 (MW 1075.16 g/mol), ESI-MS typically yields a primary singly-charged ion peak [M+H]+ around m/z 1076.2 or a doubly-charged ion peak [M+2H]2+ around m/z 538.6. Matching observed mass to theoretical structure rules out isomeric or mis-sequenced peptides.
Bacterial endotoxins (lipopolysaccharides, LPS) released from Gram-negative bacterial expression systems or environmental synthesis pathways represent a major confounding variable in laboratory assays. High endotoxin levels induce non-specific immune signaling, cytokine release, and cell death in vitro, invalidating experimental data.
PX1 Research utilizes Kinetic Chromogenic Limulus Amebocyte Lysate (LAL) assays to measure endotoxin levels. All lot-specific COAs report endotoxin concentrations below strictly enforced limits (<0.01 EU/µg). To learn more about how endotoxin controls protect assay integrity, inspect our technical overview on endotoxin testing in peptides.
When designing comparative neurological or cell-signaling experiments, researchers frequently compare SNAP-8 against other peptides targeting membrane fusion and vesicular release pathways. Below is a comparative overview of common biomimetic laboratory compounds.
SNAP-8 (Acetyl Octapeptide-3) was engineered as an extension of Argireline (Acetyl Hexapeptide-8). While Acetyl Hexapeptide-8 consists of a 6-amino-acid motif, SNAP-8 contains 8 amino acids, offering enhanced steric hindrance in SNARE complex assembly models in vitro. Additionally, researchers often evaluate complementary peptides such as Leuphasyl (Pentapeptide-18), which operates through an enkephalin-mimicking pathway to modulate neuronal excitability through distinct receptor mechanisms. Evaluating COA metrics across these compounds ensures standardized molar concentrations during comparative assays.
To maintain the chemical integrity indicated on the certificate of analysis, laboratory personnel must follow proper storage and reconstitution procedures. Lyophilized SNAP-8 is stable at room temperature for short transport durations but must be stored long-term at -20°C to -80°C in a desiccated environment.
Reconstitution Procedures: 1. Centrifuge the vial prior to opening to consolidate lyophilized powder at the bottom. 2. Reconstitute using sterile bacteriostatic water, sterile normal saline (0.9% NaCl), or phosphate-buffered saline (PBS, pH 7.4). 3. Avoid violent agitation or vortexing; gently invert or swirl the vial until dissolved. 4. Prepare single-use laboratory aliquots to avoid repeated freeze-thaw cycles, which degrade peptide bonds over time.
For step-by-step mathematical calculations regarding molarity and concentration adjustments, access our peptide reconstitution calculator. You can also browse our complete selection of research materials in the all peptides catalog.
PX1 Research maintains rigorous quality management protocols across our entire production chain. Every batch of SNAP-8 is synthesized in US-based, GMP-compliant facilities and subjected to independent analytical validation by third-party ISO 17025 accredited laboratories.
Our quality commitment includes: - Full lot traceability: Every vial is tied to a unique batch number linked directly to its downloadable COA. - Dual analytical verification: RP-HPLC for purity and ESI-MS for structural confirmation. - Rapid fulfillment: Orders ship directly from our state-of-the-art dispatch centers in California and Arizona with same-day shipping for orders placed Monday through Friday.
To review our analytical standards or submit institutional verification requests, visit the centralized PX1 Research Hub or contact our technical support team.
For academic institutions, biotechnology organizations, and high-throughput screening facilities requiring bulk quantities of SNAP-8, PX1 Research offers customized wholesale account management. Bulk orders receive batch-wide analytical validation to ensure absolute uniformity across large-scale experimental runs.
To establish a commercial or institutional research account and request high-volume lot documentation, submit an inquiry through our dedicated wholesale laboratory portal.
What is a snap-8 certificate of analysis?
A snap-8 certificate of analysis (COA) is an official laboratory document produced by an independent analytical testing facility. It verifies the chemical identity, purity percentage (via RP-HPLC), molecular mass (via ESI-MS), and endotoxin concentration of a specific batch of SNAP-8 (Acetyl Octapeptide-3).
How do I download the SNAP-8 COA for my specific batch?
You can download the batch-specific COA directly from the PX1 Research product page by matching the lot number printed on your vial's label with the public COA repository on our website.
What purity level is required on a SNAP-8 COA for research applications?
For reliable preclinical and in vitro research, SNAP-8 should demonstrate an RP-HPLC purity of ≥98.0%. Lower purity levels introduce uncharacterized synthetic impurities that can distort cellular signaling data.
How is molecular weight verified on the SNAP-8 certificate of analysis?
Molecular weight is verified using Electrospray Ionization Mass Spectrometry (ESI-MS). The analytical spectrum confirms the observed mass-to-charge ratio (m/z) matches the theoretical molecular weight of Acetyl Octapeptide-3 (1075.16 g/mol).
What endotoxin limit is acceptable on a SNAP-8 certificate of analysis?
PX1 Research enforces strict endotoxin thresholds of <0.01 EU/µg, measured via Kinetic Chromogenic LAL testing, ensuring the compound is suitable for sensitive cell culture assays without causing non-specific inflammatory responses.
Are PX1 Research SNAP-8 batches tested by a third party?
Yes. Every lot of SNAP-8 is independently analyzed by an ISO 17025 accredited analytical laboratory in the United States to ensure objective, uncompromised quality reporting.
Why is trifluoroacetate (TFA) listed on the SNAP-8 COA?
TFA is a standard mobile phase additive used during solid-phase peptide synthesis and RP-HPLC purification. It acts as a counterion to stabilize basic amino acid residues (such as Arginine) in the SNAP-8 sequence.
How should SNAP-8 be stored upon receipt to maintain COA specifications?
Lyophilized SNAP-8 powder should be stored at -20°C to -80°C in a dry container. Once reconstituted, solution aliquots should be frozen to prevent hydrolytic degradation.
What is the sequence of SNAP-8 verified on the COA?
The verified primary amino acid sequence of SNAP-8 is Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 (Acetyl Octapeptide-3).
Can I obtain bulk volume lot testing for institutional orders?
Yes. Academic and industrial laboratories ordering SNAP-8 in bulk can request specialized multi-lot testing and dedicated COA documentation through the PX1 Research wholesale department.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.