How do I calculate insulin syringe units for a peptide dose?

Concentration (mg/mL) = vial strength (mg) ÷ bacteriostatic water (mL). Draw volume (mL) = dose (mg) ÷ concentration. On a U-100 syringe, units = draw volume × 100.

How much bacteriostatic water should I add to a 10 mg vial?

2 mL is the most common choice for a 10 mg vial, giving 5 mg/mL. More water makes small doses easier to measure accurately without changing the total peptide in the vial.

How long is a reconstituted research peptide stable?

Reconstituted with bacteriostatic water and stored at 2-8 °C away from light, most research peptides are handled within about 3-4 weeks. Sealed lyophilized vials are far more stable.

Peptide Reconstitution Calculator

Enter your vial strength, the bacteriostatic water you plan to add and your target dose. The calculator returns the exact concentration, draw volume, insulin-syringe tick marks and doses per vial, plus step-by-step reconstitution instructions.

Peptide reconstitution calculator

Vial strength (mg)

Bacteriostatic water (mL)

Target dose

Concentration

5 mg/mL

Draw volume

0.05 mL

Insulin syringe

5 units (IU)

U-100 insulin syringe (100 units = 1 mL)

5 IU

020406080100

1. Wipe both stoppers with alcohol. Draw 2 mL of bacteriostatic water.
2. Inject the water slowly down the inside wall of the 10 mg vial — never directly onto the powder cake.
3. Swirl gently until fully dissolved. Do not shake.
4. Concentration is 5 mg/mL. For a 250 mcg dose draw to 5 units.
5. Store reconstituted vial at 2–8 °C, protected from light. Approx. 40 doses per vial.

Order bacteriostatic water & supplies

For laboratory and research use only. Not for human or veterinary use.

Frequently asked questions

How do I calculate insulin syringe units for a peptide dose?: Concentration (mg/mL) = vial strength (mg) ÷ bacteriostatic water (mL). Draw volume (mL) = dose (mg) ÷ concentration. On a U-100 syringe, units = draw volume × 100.
How much bacteriostatic water should I add to a 10 mg vial?: 2 mL is the most common choice for a 10 mg vial, giving 5 mg/mL. More water makes small doses easier to measure accurately without changing the total peptide in the vial.
How long is a reconstituted research peptide stable?: Reconstituted with bacteriostatic water and stored at 2-8 °C away from light, most research peptides are handled within about 3-4 weeks. Sealed lyophilized vials are far more stable.