5-Amino-1MQ 5mg — Vial Spec, Reconstitution Math & COA
A 5mg vial of 5-Amino-1MQ offers precise mass calibration for small-scale laboratory assays, cell culture screening, and pilot metabolic evaluations. As a selective nicotinamide N-methyltransferase (NNMT) inhibitor, 5-Amino-1MQ is studied for its ability to raise cellular NAD+ levels, improve mitochondrial output, and support fat-metabolism research. This guide details liquid reconstitution mathematics, aliquot planning, analytical specifications, and lot-matched verification for laboratory researchers.
Quick answer
A 5mg vial of 5-Amino-1MQ offers precise mass calibration for small-scale laboratory assays, cell culture screening, and pilot metabolic evaluations. As a selective nicotinamide N-methyltransferase (NNMT) inhibitor, 5-Amino-1MQ is studied for its ability to raise cellular NAD+ levels, improve mitochondrial output, and support fat-metabolism research. This guide details liquid reconstitution mathematics, aliquot planning, analytical specifications, and lot-matched verification for laboratory researchers.
Key takeaways
- A 5mg vial of [5-Amino-1MQ](/research-peptides/5-amino-1mq) contains 5 milligrams of high-purity, lyophilized 5-amino-1-methylquinolinium salt formulated exclusively for in vitro research and preclinical laboratory protocols.
- [5-Amino-1MQ](/research-peptides/5-amino-1mq) functions as a membrane-permeable, highly selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT).
- In vitro assays and rodent models demonstrate that inhibiting NNMT via [5-Amino-1MQ](/research-peptides/5-amino-1mq) induces significant remodeling of cellular bioenergetics.
- Achieving accurate target concentrations requires rigorous volumetric calculations when diluting a 5mg mass format.
Overview of 5-Amino-1MQ 5mg Vials and Laboratory Applications
A 5mg vial of 5-Amino-1MQ contains 5 milligrams of high-purity, lyophilized 5-amino-1-methylquinolinium salt formulated exclusively for in vitro research and preclinical laboratory protocols. Principal investigators and lab technicians frequently select the 5mg unit size for low-volume dose-response screens, acute enzyme inhibition assays, or pilot cell-culture studies where long-term storage of reconstituted liquid solutions is unnecessary or undesirable.
By utilizing a calibrated 5-Amino-1MQ 5mg vial, research teams can minimize compound waste and prevent stability degradation caused by repeated freeze-thaw cycles. Should an exact 5mg configuration be unavailable during specific manufacturing cycles, PX1 Research offers analytical-grade mass alternatives across our broader catalog of research peptides and small molecules to ensure seamless continuity in laboratory workflows.
Molecular Mechanism: NNMT Inhibition and Cellular Energy Dynamics
5-Amino-1MQ functions as a membrane-permeable, highly selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme responsible for catalyzing the methylation of nicotinamide (NAM) utilizing S-adenosylmethionine (SAM) as the primary methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH).
In metabolic tissues such as white adipose tissue and the liver, overexpression of NNMT depletes intracellular pools of salvageable nicotinamide, thereby restricting the enzymatic production of nicotinamide adenine dinucleotide (NAD+). Direct inhibition of NNMT by 5-Amino-1MQ prevents the irreversible clearance of nicotinamide. Preclinical studies suggest that preserving intracellular NAM and SAM concentrations drives salvage pathway kinetics, promoting NAD+ resynthesis via nicotinamide phosphoribosyltransferase (NAMPT) activity without inducing systemic cytotoxicity.
Preclinical Research Findings: NAD+ Dynamics and Mitochondrial Output
In vitro assays and rodent models demonstrate that inhibiting NNMT via 5-Amino-1MQ induces significant remodeling of cellular bioenergetics. Experimental data indicate that the resulting increase in intracellular NAD+ availability elevates the activity of NAD+-dependent deacetylases, particularly sirtuin-1 (SIRT1). SIRT1 activation subsequently promotes the deacetylation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), a master regulator of mitochondrial biogenesis.
In cell culture models, treatment with NNMT inhibitors is correlated with increased mitochondrial mass, elevated basal oxygen consumption rates (OCR), and enhanced extracellular acidification rates (ECAR). Furthermore, in animal models of diet-induced metabolic impairment, 5-Amino-1MQ administration has been observed to alter adipocyte morphology, stimulate substrate oxidation, and support fat-metabolism research goals without altering baseline food intake. These findings position the compound as a key probe for investigating energy expenditure mechanisms.
Reconstitution Calculations and Volumetric Math for 5mg Vials
Achieving accurate target concentrations requires rigorous volumetric calculations when diluting a 5mg mass format. Laboratory personnel are advised to utilize our interactive reconstitution calculator prior to fluid handling to confirm stock solution density.
The following standard reconstitution volumes illustrate final concentration outputs for a 5mg total solute mass: Adding 1.0 mL of liquid diluent yields a final stock concentration of 5.0 mg/mL (5.0 µg/µL), which is highly suited for micro-volume additions where solvent volume must be minimized. Adding 2.0 mL of diluent produces a concentration of 2.5 mg/mL (2.5 µg/µL), providing an ideal compromise between pipetting accuracy and volumetric flexibility. Adding 3.0 mL of diluent results in approximately 1.67 mg/mL (1.67 µg/µL), facilitating low-concentration assays where microgram-level delivery requires larger, highly repeatable volumetric pipetting.
Aliquot Planning, Diluent Options, and Handling Protocols
Unlike standard hydrophilic peptides that readily dissolve in sterile water, small-molecule quinolinium derivatives like 5-Amino-1MQ may exhibit unique solubility parameters based on vehicle pH and organic solvent content. Laboratory protocols typically employ dimethyl sulfoxide (DMSO) or specialized aqueous buffers such as phosphate-buffered saline (PBS) to achieve complete solubilization at high stock concentrations.
To prevent degradation associated with multiple freeze-thaw cycles, working stocks should be split into single-use aliquots immediately after reconstitution. Handling must take place under sterile laminar flow hoods using low-retention polypropylene microcentrifuge tubes. Researchers can consult our preclinical research database for specific vehicle compatibility matrices and cell culture solvent tolerance thresholds.
Storage Stability, Lyophilization Specs, and Reconstituted Shelf Life
Prior to reconstitution, lyophilized 5-Amino-1MQ powder exhibits long-term stability when stored in a desiccated environment at -20°C to -80°C, isolated from direct light. PX1 Research utilizes optimized freeze-drying protocols to produce stable, cake-like solid matrices with minimal residual moisture, preventing spontaneous hydrolysis during storage.
Once in liquid solution, chemical stability depends heavily on the chosen vehicle and ambient temperature. Aqueous working solutions should be stored at 2°C to 8°C and evaluated within short experimental timeframes (24 to 72 hours). For extended storage, concentrated DMSO aliquots maintained at -80°C remain structurally stable over prolonged research timelines, provided moisture ingress is strictly controlled.
Purity Verification, HPLC/MS Analysis, and Lot-Matched COAs
To ensure experimental reproducibility, every batch of 5-amino-1mq 5mg distributed by PX1 Research undergoes stringent analytical verification at an independent, ISO 17025 accredited laboratory. Analytical testing protocols include High-Performance Liquid Chromatography (HPLC) to establish chromatographic purity and Mass Spectrometry (MS) to verify molecular weight and structural identity.
Researchers can review and download lot-matched documentation directly from our lot-matched COA portal. Additionally, assays confirm that bacterial endotoxin levels remain well below critical thresholds, preventing non-specific inflammatory signaling or artifact generation in sensitive cell culture models.
Comparative Analysis: 5-Amino-1MQ vs. Related Metabolic Research Compounds
In metabolic signaling research, 5-Amino-1MQ is evaluated alongside distinct small molecules and peptide probes that influence cellular bioenergetics. While 5-Amino-1MQ targets the cytosolic NNMT pathway to elevate intracellular NAD+ pools, compounds such as MOTS-c act via mitochondrial-derived peptide pathways to induce AMP-activated protein kinase (AMPK) phosphorylation. In contrast, synthetic agonists like SLU-PP-332 target estrogen-related receptors (ERRs) to promote oxidative gene expression in skeletal muscle, whereas PPAR-delta agonists such as GW501516 modulate fatty acid transport and beta-oxidation cascades.
Evaluating these distinct mechanisms within comparative in vitro assays allows researchers to map overlapping nodes of cellular energy regulation, mitochondrial biogenesis, and metabolic substrate switching.
Selecting Mass Formats: Comparing 5mg to Larger Unit Sizes
Selecting between a 5mg vial format and larger mass sizes depends primarily on the scope, scale, and duration of the planned research protocol. The 5mg mass format is ideal for preliminary feasibility studies, enzyme kinetics baseline assays, or low-throughput cell culture screens where minimizing reagent degradation over time is paramount.
For high-throughput screening campaigns, multi-plate cell culture series, or extended preclinical animal studies, laboratories often benefit from procuring higher mass single-vial formats or opening bulk lab accounts. Utilizing larger single-lot quantities ensures consistency across multi-week protocols while reducing unit costs for academic and industrial research facilities.
PX1 Research Sourcing, Quality Control, and Supply Protocols
PX1 Research is a trusted USA-based supplier committed to serving institutional, university, and private research laboratories with analytical-grade compounds. All products are synthesized within state-of-the-art, GMP-compliant manufacturing facilities and subjected to multi-stage quality control checks.
Operating fulfillment centers out of California and Arizona, PX1 Research provides same-day shipping on orders placed Monday through Friday prior to regional cut-off times. All compounds are packaged in heavy-wall borosilicate glass vials with crimp seals to preserve chemical integrity during transport, ensuring reliable delivery to laboratory benches nationwide.
Frequently Asked Questions
What is the specific target of 5-Amino-1MQ in research assays?
5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT), a cytosolic enzyme involved in methyl donor balance and NAD+ clearance.
How is a 5mg 5-Amino-1MQ vial reconstituted for bench experimentation?
The compound is typically dissolved using sterile DMSO or specialized laboratory buffers depending on downstream cell culture solvent tolerances.
What concentrations are produced when diluting 5mg in 1mL, 2mL, or 3mL of liquid?
Adding 1.0 mL yields 5.0 mg/mL, adding 2.0 mL yields 2.5 mg/mL, and adding 3.0 mL yields approximately 1.67 mg/mL.
How does PX1 Research verify compound purity?
Every lot is independently tested in an ISO 17025 accredited laboratory using HPLC for purity verification (>98%) and Mass Spectrometry for identity confirmation.
Where can researchers obtain the lot-matched COA for their 5-Amino-1MQ order?
Certificates of Analysis are accessible online via the PX1 Research COA portal by entering the batch number printed on the vial label.
Is 5-Amino-1MQ approved for human administration or therapeutic use?
No. 5-Amino-1MQ is strictly a laboratory research compound intended for in vitro assays and animal models. Human or veterinary use is strictly prohibited.
What are the recommended storage conditions for lyophilized 5-Amino-1MQ?
Lyophilized vials should be stored desiccated at -20°C to -80°C and protected from light to maintain long-term molecular stability.
Are PX1 Research compounds tested for bacterial endotoxins?
Yes, all production lots undergo stringent endotoxin testing to ensure suitablity for cell culture and sensitive biological assays.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.