PX1 Research provides synthetic N-Acetyl Epitalon synthesized to ≥98% purity for advanced preclinical research into pineal bioregulation, telomerase activation, and cellular aging models. Every batch is manufactured in USA-based GMP-compliant facilities and rigorously verified by independent ISO 17025 accredited laboratories.
PX1 Research provides synthetic N-Acetyl Epitalon synthesized to ≥98% purity for advanced preclinical research into pineal bioregulation, telomerase activation, and cellular aging models. Every batch is manufactured in USA-based GMP-compliant facilities and rigorously verified by independent ISO 17025 accredited laboratories.
Researchers seeking to buy N-Acetyl Epitalon require reference-standard materials characterized by precise molecular composition, verifiable purity, and strict endotoxin control. N-Acetyl Epitalon is a synthetic acetylated derivative of the pineal tetrapeptide Epitalon (Ala-Glu-Asp-Gly). It is synthesized exclusively for laboratory evaluation in vitro and in non-human animal models investigating telomerase dynamics, chromatin structure, and neuroendocrine signaling pathways.
PX1 Research delivers reference-grade N-Acetyl Epitalon formulated to support reproducible scientific assays. Each lot undergoes comprehensive analytical characterization, ensuring research institutions receive compounds verified via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Electrospray Ionization Mass Spectrometry (ESI-MS). Samples ship directly from our domestic facilities in California and Arizona to streamline logistics for accredited research laboratories.
N-Acetyl Epitalon is structurally defined as N-acetyl-L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine. The chemical introduction of an acetyl moiety at the N-terminal alanine residue alters the peptide's overall dipole moment, lipophilicity, and enzymatic stability compared to its unacetylated counterpart. In biological fluids, un-modified short peptides frequently face rapid hydrolysis by endo- and exopeptidases. Preclinical models suggest N-terminal acetylation provides enzymatic resistance against aminopeptidases, extending the functional half-life of the molecule during cell culture incubations and tissue assays.
The molecular formula of N-Acetyl Epitalon is C16H24N4O10 with a theoretical molecular mass of approximately 432.38 g/mol. This modification preserves the essential acidic side-chain architecture formed by the glutamic and aspartic acid residues, which are hypothesized to interact directly with nuclear histones and specific DNA promoter regions. Understanding these structural dynamics is critical for researchers investigating transcriptional activation of catalytic sub-units in preclinical cell models.
The primary line of investigation surrounding N-Acetyl Epitalon focuses on its potential role as a peptide bioregulator capable of modulating gene expression. In vitro data indicate that short peptide sequences matching pineal extracts can bind to the major and minor grooves of double-stranded DNA, as well as histone proteins. This interaction is hypothesized to induce local chromatin decondensation, rendering promoter regions accessible to RNA polymerase II and specific transcription factors.
A major area of study involves the telomerase reverse transcriptase (TERT) gene. Telomerase is the ribonucleoprotein complex responsible for adding telomeric repeats (TTAGGG) to the 3' ends of chromosomes, preserving chromosomal integrity during mitotic divisions. Preclinical studies suggest that exposure to synthetic pineal bioregulators like Epitalon and its acetylated analogue N-Acetyl Epitalon correlates with increased TERT mRNA expression and enhanced telomerase enzymatic activity in somatic cell cultures. Researchers utilize these models to evaluate cellular senescence markers, hayflick limit extensions, and telomere length maintenance over successive cell passages.
Beyond chromosomal architecture, N-Acetyl Epitalon is extensively studied in the context of pineal gland biology and neuroendocrine regulation. The pineal gland plays a central role in orchestrating systemic circadian rhythms through the rhythmic synthesis and secretion of melatonin. As organisms age, pineal involution and calcification frequently lead to a marked decline in nocturnal melatonin production, disrupting downstream circadian signaling and antioxidant cascades.
In rodent models of accelerated aging, pineal-derived peptide bioregulators have been evaluated for their capacity to restore physiological pineal morphology and normalize melatonin synthesis pathways. In vitro assays using pinealocyte cultures demonstrate that treatment with synthetic bioregulators can upregulate key enzymes in the melatonin biosynthetic pathway, such as serotonin N-acetyltransferase (AANAT). Investigating these neuroendocrine pathways provides valuable insights into how short signaling peptides influence central circadian pacemakers and systemic homeostasis in animal models.
When designing comparative research protocols, investigators frequently evaluate N-Acetyl Epitalon alongside other short synthetic bioregulators within our research peptides catalog. Understanding the structural and functional nuances between these molecules allows for precise control of experimental parameters across different tissue targets.
For instance, while Epitalon represents the native tetrapeptide sequence, the N-acetyl derivative offers altered stability profiles in peptidase-rich media. In contrast, tripeptides such as Pinealon (Glu-Asp-Arg) target distinct neuronal gene networks and focus heavily on neuroprotective and synaptic plasticity pathways. Similarly, immunomodulatory research often incorporates Thymalin, a thymic peptide fraction studied for T-cell differentiation and immune system bioregulation. Comparing these distinct bioregulatory peptides enables researchers to map cell-type-specific transcriptional responses across various tissue models.
The integrity of scientific research depends fundamentally on the chemical purity and consistency of reagents. PX1 Research enforces rigorous quality control standards for every lot of N-Acetyl Epitalon manufactured in our cGMP-compliant US facilities. Qualified buyers are provided with comprehensive, lot-specific Certificates of Analysis (COAs) generated by independent ISO 17025 accredited analytical laboratories.
Purity assessment is primarily performed using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). This process separates the primary target peptide from synthesis byproducts, truncated sequences, and residual protecting groups, ensuring an analytical purity profile of ≥98.0%. Complementary Electrospray Ionization Mass Spectrometry (ESI-MS) confirms the exact molecular weight and verifies structural identity. Researchers can review sample analytical reports directly through our research library hub prior to procuring materials for experimental use.
Bacterial endotoxins—lipopolysaccharides (LPS) derived from the outer membrane of Gram-negative bacteria—pose a significant risk of confounding experimental results. Even trace amounts of endotoxin in peptide preparations can trigger unspecific inflammatory cascades, alter gene expression profiles, and induce cell death in sensitive primary cell lines.
To protect research validity, every batch of N-Acetyl Epitalon supplied by PX1 Research undergoes strict endotoxin quantitation utilizing kinetic Chromogenic Reagent Limulus Amebocyte Lysate (LAL) testing. We enforce a stringent threshold of <0.5 EU/mg, making our compounds suitable for sensitive in vitro assays, organoid cultures, and in vivo animal models. Detailed endotoxin data are published directly on each product's COA.
Proper reconstitution of lyophilized N-Acetyl Epitalon is essential to maintain structural integrity and prevent aggregation or degradation. Reconstitution should always take place inside a validated laminar flow biosafety cabinet using aseptic technique. Investigators must select an appropriate solvent based on the specific requirements of their downstream experimental assays.
For most biological assays, sterile Bacteriostatic Water (containing 0.9% benzyl alcohol) or sterile Phosphate-Buffered Saline (PBS, pH 7.4) is recommended. The lyophilized cake should be allowed to equilibrate to room temperature before adding the diluent along the inner glass wall of the vial. Gently swirl the vial to facilitate complete dissolution; mechanical agitation such as vigorous shaking must be avoided to prevent protein shearing or foam formation. For bulk experimental preparation, researchers can consult our wholesale lab account portal for specialized reagent volumes and bulk ordering support.
Lyophilized N-Acetyl Epitalon is stable at room temperature for brief periods during transit, but long-term preservation requires controlled environmental conditions. Upon receipt, lyophilized vials should be stored in a desiccated environment at -20°C or -80°C to prevent moisture absorption and enzymatic degradation.
Once reconstituted into solution, N-Acetyl Epitalon exhibits reduced long-term stability. Reconstituted stock solutions should be divided into single-use microcentrifuge aliquots to eliminate damaging freeze-thaw cycles. Aliquoted solutions stored at 4°C should typically be utilized within 7 to 14 days, whereas frozen aliquots stored at -20°C remain viable for up to 3 to 6 months depending on the solvent system and concentration.
PX1 Research prioritizes efficient, reliable delivery to support tight academic and corporate research schedules. All orders placed Monday through Friday are processed with same-day shipping options from our centralized distribution hubs in California and Arizona. Vials are packaged in heavy-duty temperature-insulated containers designed to preserve physical state and stability throughout transit.
Our institution-focused platform caters to single-vial analytical needs as well as large-scale screening campaigns. Principal investigators and laboratory procurement managers seeking high-volume supplies can utilize our wholesale program to obtain dedicated account management, custom synthesis options, and standardized batch reservations for multi-phase longitudinal studies.
What is the primary difference between Epitalon and N-Acetyl Epitalon?
N-Acetyl Epitalon features an added acetyl group at the N-terminal alanine residue. In preclinical research models, this acetylation is studied for providing enhanced enzymatic resistance against peptidase degradation, potentially extending the compound's half-life in vitro compared to unmodified Epitalon.
How does PX1 Research verify the purity of N-Acetyl Epitalon?
Every lot of N-Acetyl Epitalon undergoes independent testing at ISO 17025 accredited laboratories. Purity is established via RP-HPLC (verifying ≥98% purity), identity is confirmed using ESI-MS, and endotoxin levels are quantified using kinetic LAL assays.
Is N-Acetyl Epitalon suitable for human consumption or administration?
No. N-Acetyl Epitalon supplied by PX1 Research is strictly designated for laboratory research use only. It is not intended for human or animal medical, diagnostic, therapeutic, or personal use.
What solvent is recommended for reconstituting N-Acetyl Epitalon in cell culture assays?
For cell culture and in vitro experiments, sterile Phosphate-Buffered Saline (PBS) or sterile water for injection is typically utilized. If microbial control over extended periods is required for non-cellular assays, Bacteriostatic Water (0.9% benzyl alcohol) may be used.
What are the endotoxin limits for PX1 Research bioregulators?
PX1 Research enforces a strict endotoxin specification of <0.5 EU/mg for N-Acetyl Epitalon, ensuring the material is suitable for sensitive preclinical assays without risking LPS-induced artifactual data.
How should reconstituted N-Acetyl Epitalon stock solutions be stored?
Reconstituted solutions should be divided into single-use aliquots to avoid freeze-thaw cycles. Short-term storage (under 14 days) should be maintained at 4°C, while long-term storage of aliquots should be held at -20°C or -80°C.
From where are PX1 Research compounds shipped?
All orders are fulfilled directly from our domestic dispatch centers located in California and Arizona, with same-day shipping available for orders processed Monday through Friday.
Which related bioregulators are commonly compared to N-Acetyl Epitalon in literature?
Preclinical literature often compares N-Acetyl Epitalon to its parent compound Epitalon, the neuro-focused tripeptide Pinealon, and the thymic immune bioregulator Thymalin.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.