Vilon is a short synthetic bioregulatory dipeptide composed of L-lysine and L-glutamic acid (Lys-Glu), extensively studied in preclinical models for its role in chromatin unfolding, transcriptional activation, and cellular regeneration. PX1 Research supplies research-grade Vilon manufactured in USA-based, GMP-compliant facilities with independent lot-specific HPLC and MS analytical validation for in vitro and laboratory investigations.
Vilon is a short synthetic bioregulatory dipeptide composed of L-lysine and L-glutamic acid (Lys-Glu), extensively studied in preclinical models for its role in chromatin unfolding, transcriptional activation, and cellular regeneration. PX1 Research supplies research-grade Vilon manufactured in USA-based, GMP-compliant facilities with independent lot-specific HPLC and MS analytical validation for in vitro and laboratory investigations.
Researchers looking to buy Vilon for liver recovery studies evaluate this synthetic dipeptide (Lys-Glu) for its capacity to modulate gene expression, chromatin conformation, and cellular repair pathways in hepatic tissue models. PX1 Research supplies high-purity, USA-synthesized Vilon featuring lot-specific HPLC and mass spectrometry verification strictly for in vitro and animal research applications.
When purchasing bioregulatory compounds like Vilon for experimental setups, maintaining strict quality controls is vital. Tissue culture systems, primary hepatocyte assays, and rodent injury models depend heavily on peptide stability, precise stoichiometry, and complete freedom from cytotoxic impurities. Through our rigorous testing protocols, PX1 Research ensures that every vial of Vilon meets stringent purity benchmarks (>99% pure by RP-HPLC), allowing laboratory investigators to achieve reproducible results across longitudinal assays.
In experimental models of hepatic stress, ischemic damage, and toxic challenge, short bioregulatory peptides are studied to observe how ultrashort amino acid sequences interact with nuclear matrices. Research teams seeking to purchase research peptides for organ-specific repair models rely on PX1 Research for batch-to-batch consistency, transparent analytical documentation, and rapid domestic shipping.
Vilon is a short peptide consisting of two amino acid residues: L-lysine and L-glutamic acid (Lys-Glu). With a low molecular mass of approximately 275.3 g/mol, its small footprint allows rapid diffusion across cellular membranes and potential nuclear translocation in cellular models.
The molecular structure of Vilon features a positively charged basic side chain from lysine paired with an acidic, negatively charged side chain from glutamic acid. This zwitterionic structure at physiological pH endows the dipeptide with unique binding affinity for histone proteins and specific non-coding genomic regions. In contrast to larger oligopeptides or complex proteins, short dipeptides maintain high chemical stability in aqueous environments, making them ideal subjects for structural biology and biophysical interaction assays.
Because of its simple chemical sequence, synthetic production of Vilon requires precise solid-phase peptide synthesis (SPPS) parameters to avoid racemic mixtures or residual coupling reagents. PX1 Research utilizes advanced SPPS methodologies to ensure that every lot of Vilon exhibits exact stereochemical purity and uniform molecular mass confirmation via Electrospray Ionization Mass Spectrometry (ESI-MS).
Preclinical literature demonstrates significant interest in Vilon’s capacity to affect hepatocyte viability and protein synthesis under simulated metabolic or toxic stress. In rodent models of liver damage—including carbon tetrachloride (CCl4)-induced hepatotoxicity and partial hepatectomy models—preclinical studies suggest that short bioregulatory peptides influence rate-limiting enzymatic pathways involved in cellular repair.
In vitro data indicate that exposure of cultured hepatocytes to short dipeptides enhances ribosomal RNA synthesis and stimulates chromatin decondensation. These intracellular shifts correlate with increased expression of endogenous antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, which help defend hepatic parenchymal cells against oxidative damage.
Furthermore, researchers investigating hepatic fibrosis models analyze how Lys-Glu influences extracellular matrix (ECM) remodeling. In cellular co-cultures of hepatocytes and hepatic stellate cells, researchers monitor changes in transforming growth factor-beta (TGF-β) signaling and collagen deposition. These preliminary findings highlight Vilon’s utility as a molecular probe for investigating organ-specific repair pathways in our broader peptide research hub.
The primary mechanism of action attributed to short peptide bioregulators like Vilon is their direct interaction with condensed heterochromatin. Structural studies suggest that ultrashort amino acid chains enter cell nuclei and insert into the major or minor grooves of DNA, as well as binding to histone tails.
This molecular binding destabilizes ionic interactions between negatively charged DNA backbones and positively charged histone proteins, inducing localized chromatin unfolding (euchromatin formation). By relaxing dense heterochromatin structures, Vilon enables RNA polymerase complexes and transcription factors to access previously silenced or suppressed gene promoters.
In hepatic models, this epigenetic derepression leads to altered transcriptional profiles for genes regulating cell cycle progression, anti-apoptotic signals, and tissue-specific metabolic enzymes. Investigators utilize Vilon to explore how epigenetic modulation can be harnessed to stimulate endogenous cellular recovery mechanisms without altering the underlying genetic sequence.
To contextualize Vilon within the broader landscape of bioregulatory and tissue-modulating compounds, researchers frequently compare its activity to other short synthetic peptides. While Vilon (Lys-Glu) focuses primarily on immune and parenchymal tissue responsiveness, compounds like Epitalon (Ala-Glu-Asp-Gly) operate through telomerase activation and pineal gland regulation. Similarly, Thymogen (Glu-Trp) is evaluated predominantly for T-cell differentiation and systemic immunomodulation.
When designing multi-peptide comparative assays for tissue recovery, research teams also examine broader matrix-repair peptides such as BPC-157 and GHK-Cu. While BPC-157 modulates growth factor expression (such as VEGF and EGFR) to drive angiogenesis and connective tissue healing, Vilon acts directly at the chromatin level to alter transcriptomic activity in damaged parenchymal tissues like the liver.
Understanding these mechanistic distinctions allows investigators to select the exact molecular candidate required for their hypothesis. For laboratories executing high-throughput comparative screenings, PX1 Research provides comprehensive catalog options with complete batch traceability.
Proper reconstitution of lyophilized Vilon is essential to maintain structural integrity and achieve accurate dosing concentrations in laboratory preparations. Vilon is supplied as a lyophilized white powder that exhibits high solubility in aqueous buffer systems.
For standard cell culture and biochemical protocols, researchers typically reconstitute Vilon using sterile Bacteriostatic Water (0.9% benzyl alcohol) or sterile Phosphate-Buffered Saline (PBS, pH 7.4). The recommended procedure involves introducing the solvent gently down the inner glass wall of the vial, followed by gentle swirling or inversion. High-speed vortexing or aggressive agitation should be avoided to prevent surface-induced aggregation.
Once dissolved, working solutions should be prepared under a laminar flow hood using aseptic techniques. For long-term cell culture studies where benzyl alcohol could introduce cytotoxic artifacts, sterile 0.9% Sodium Chloride or standard culture media is preferred. Researchers should always calculate stock concentrations based on the exact net peptide content specified on the lot-specific Certificate of Analysis.
To preserve the stability of Vilon and prevent degradation or hydrolysis, strict laboratory storage protocols must be maintained upon receipt of shipment.
Lyophilized peptide vials should be stored in a freezer at -20°C for short to medium-term storage, or at -80°C for extended research periods. Under these temperature conditions, lyophilized Vilon remains stable for up to 24 months. Vials should be kept away from ambient light and moisture exposure inside sealed containers containing desiccant packs.
Following reconstitution, liquid aliquots should be used immediately or frozen in single-use working volumes at -20°C or -80°C. Repeated freeze-thaw cycles must be strictly avoided, as thermal fluctuations promote peptide cleavage and atmospheric moisture condensation. Reconstituted aqueous solutions stored at 2°C to 8°C should be utilized within 7 to 14 days depending on the solvent matrix and sterility conditions.
In cell culture assays—particularly primary hepatocyte studies—the presence of residual solvents, truncated synthesis fragments, or bacterial endotoxins can obscure experimental results and induce non-specific cell death. Consequently, third-party analytical verification is non-negotiable when sourcing research materials.
PX1 Research subjects every batch of Vilon to rigorous analytical testing by ISO 17025 accredited independent laboratories. Chemical purity is quantified using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), ensuring a purity threshold of ≥98% (and frequently exceeding 99%). Mass identity is confirmed via ESI-MS to ensure exact molecular weight concordance without underlying synthesis contaminants.
In addition, endotoxin levels are verified through Limulus Amebocyte Lysate (LAL) testing, guaranteeing endotoxin levels below 0.01 EU/mg. This level of verification ensures that observed biological responses in cellular recovery assays are directly attributable to the Vilon molecule rather than inflammatory contaminants.
PX1 Research is committed to providing academic institutions, biotechnology companies, and independent researchers with high-grade, domestic-synthesized research peptides. All compounds in our catalog are manufactured in state-of-the-art USA facilities adhering to strict GMP compliance frameworks.
We maintain full transparency by attaching batch-specific Certificates of Analysis directly to every shipment and making analytical records accessible online. Orders are packed under controlled conditions and dispatched same-day (Monday through Friday) from our fulfillment centers located in California and Arizona, minimizing transit times and temperature exposure.
Whether your laboratory requires individual vials for initial pilot studies or bulk procurement via our wholesale accounts platform, PX1 Research provides the quality control, reliability, and technical oversight necessary to support rigorous scientific discovery.
What is Vilon used for in laboratory research?
Vilon (Lys-Glu) is a short bioregulatory dipeptide used in laboratory research to investigate chromatin decondensation, gene transcription activation, cellular proliferation, and tissue repair pathways, particularly in primary hepatocyte and immune cell models.
What preclinical evidence exists regarding Vilon and liver recovery?
Preclinical studies in rodent models of chemical hepatotoxicity (e.g., CCl4 exposure) and liver resection demonstrate that short dipeptides like Vilon support hepatocyte viability, enhance protein synthesis rates, and modulate endogenous antioxidant enzyme expression (SOD and catalase).
How does PX1 Research verify the purity of Vilon?
Every lot of Vilon from PX1 Research undergoes third-party verification by an ISO 17025 accredited laboratory. Purity is confirmed to be ≥98% using Reverse-Phase HPLC, molecular identity is verified via Mass Spectrometry (ESI-MS), and endotoxin levels are measured using LAL assays.
What is the recommended reconstitution solvent for Vilon in cell culture?
For cell culture and in vitro assays, Vilon is typically reconstituted using sterile Phosphate-Buffered Saline (PBS, pH 7.4) or sterile 0.9% Sodium Chloride to avoid cytotoxic effects associated with preservative agents.
How should reconstituted Vilon be stored?
Once reconstituted, Vilon working solutions should be divided into single-use aliquots and frozen at -20°C or -80°C to prevent freeze-thaw degradation. Short-term storage at 2°C to 8°C is acceptable for up to 7–14 days depending on sterility.
How does Vilon differ from Epitalon?
Vilon is a dipeptide (Lys-Glu) focused primarily on chromatin unfolding and parenchymal tissue repair mechanisms, whereas Epitalon is a tetrapeptide (Ala-Glu-Asp-Gly) investigated for telomerase activation and neuroendocrine regulation.
Does PX1 Research ship Vilon internationally?
PX1 Research primary fulfillment operates from California and Arizona, offering fast domestic shipping across the USA. International shipping availability and compliance depend on regional destination regulations for research compounds.
Is Vilon approved for human clinical use or administration?
No. Vilon supplied by PX1 Research is strictly designated for laboratory research, in vitro studies, and preclinical scientific investigation. It is not for human or animal clinical use, therapeutic administration, or diagnostic purposes.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.