This technical document evaluates CJC-1295 DAC from an analytical and preclinical perspective for laboratory researchers. Investigate its molecular structure, extended half-life mechanisms, tissue repair research applications, and stringent pharmaceutical-grade purity criteria required for reproducible in vitro and animal models.
This technical document evaluates CJC-1295 DAC from an analytical and preclinical perspective for laboratory researchers. Investigate its molecular structure, extended half-life mechanisms, tissue repair research applications, and stringent pharmaceutical-grade purity criteria required for reproducible in vitro and animal models.
CJC-1295 DAC pharmacie refers to pharmaceutical-grade CJC-1295 conjugated with Drug Affinity Complex, a synthetic 29-amino-acid growth hormone-releasing hormone (GHRH) analog engineered with a reactive maleimidopropionic acid group. In preclinical laboratory settings, this covalent conjugation allows binding to endogenous albumin, significantly extending its biological half-life to study sustained GH and downstream IGF-1 elevation.
When laboratory investigators search for pharmaceutical-grade CJC-1295 DAC, the primary focus is establishing strict chemical purity, structural integrity, and lot-to-lot consistency. Unlike crude or unpurified chemical reagents, pharmaceutical-grade research peptides undergo high-performance liquid chromatography (HPLC) and mass spectrometry (MS) verification to ensure chemical identity and minimize background artifacts in experimental protocols.
CJC-1295 is a modified version of Growth Hormone-Releasing Factor (GRF 1-29), featuring amino acid substitutions at positions 2, 8, 15, and 27. These modification sites (D-Ala2, Gln8, Ala15, and Leu27) render the peptide resistant to rapid enzymatic cleavage by dipeptidyl peptidase-IV (DPP-IV). The addition of the Drug Affinity Complex (DAC)—specifically a maleimido derivative attached via a lysine linker at the C-terminus—fundamentally alters the pharmacokinetic profile of the peptide.
Preclinical studies suggest that upon administration in animal models, the maleimide group on the DAC construct reacts selectively with the free sulfhydryl group of Cys34 on serum albumin. This irreversible covalent bond protects the peptide from renal clearance and enzymatic degradation. Consequently, while unmodified GHRH analogs exhibit a half-life measured in minutes, CJC-1295 DAC exhibits an extended half-life of 6 to 8 days in animal models, enabling sustained growth hormone release without requiring frequent administration.
In vitro data and rodent models demonstrate that CJC-1295 DAC acts directly on pituitary somatotropes by binding to the GHRH receptor. This activation triggers the adenylate cyclase-CAMP-protein kinase A signaling cascade, facilitating the transcription and pulsatile secretion of endogenous growth hormone. Because the peptide remains bound to circulating albumin, somatotrope stimulation persists over an extended analytical window.
Research in animal models has shown that sustained GH release mediated by CJC-1295 DAC yields a corresponding increase in systemic insulin-like growth factor 1 (IGF-1) levels. Researchers examine these elevated IGF-1 dynamics in tissue repair research, exploring cellular proliferation, nitrogen retention, collagen synthesis, and skeletal muscle regeneration. Investigation into the GHRH receptor axis remains a focal point in metabolic, musculoskeletal, and endocrinological research available within our broader research library.
The designation of 'pharmacie grade' or analytical grade in peptide synthesis necessitates strict adherence to quality control parameters. Standard research chemicals may contain synthesis truncations, residual protective groups, or high concentrations of bacterial endotoxins. For rigorous scientific studies, these impurities can alter receptor binding affinity, induce cytotoxicity in cell cultures, or trigger non-specific immune responses in vivo.
PX1 Research enforces rigorous quality assurance by subjecting every lot to independent third-party testing in an ISO 17025 accredited laboratory. Purity is validated via Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC), ensuring a purity profile exceeding 99%. Identity is confirmed via electrospray ionization mass spectrometry (ESI-MS), verifying precise molecular weight. Furthermore, bacterial endotoxins are quantified using chromogenic LAL assays to ensure levels remain well below critical thresholds (<0.01 EU/mg).
When evaluating GHRH analogs and growth hormone secretagogues for laboratory protocols, researchers must select compounds based on desired biological half-life and receptor specificity. The presence or absence of the Drug Affinity Complex fundamentally dictates the experimental design.
For instance, CJC-1295 No DAC (also known as Modified GRF 1-29) shares the identical tetra-substituted 29-amino-acid backbone but lacks the maleimide linker. As a result, CJC-1295 No DAC exhibits a brief half-life (approximately 30 minutes in rodent models), producing short, physiological GH spikes. Conversely, Sermorelin represents the truncated 1-29 sequence without DPP-IV stabilizing substitutions, resulting in rapid hepatic and renal clearance. Investigators studying synergism often pair GHRH analogs with growth hormone secretagogue receptor (GHSR) agonists like Ipamorelin to evaluate dual-receptor activation on pituitary somatotropes. Researchers can explore these distinct profiles across our full catalog of all peptides.
Lyophilized CJC-1295 DAC exhibits superior thermal stability compared to liquid formulations, but appropriate storage protocols must be maintained to prevent hydrolysis or oxidation. Upon receipt, lyophilized peptide vials should be stored in a controlled temperature environment at -20°C or -80°C for long-term storage, protected from light exposure.
Prior to opening, peptide vials must be allowed to equilibrate to room temperature to prevent condensation from forming inside the vial. Unopened lyophilized cakes stored at -20°C remain stable for up to 24 months. Researchers seeking high-volume supplies for ongoing laboratory studies can access institutional pricing and volume options through a wholesale research account.
Reconstitution of CJC-1295 DAC must be performed under aseptic conditions using sterile laboratory diluents such as Bacteriostatic Water (0.9% benzyl alcohol) or Sterile Normal Saline, depending on experimental requirements. Gently stream the diluent down the inner glass wall of the vial rather than shooting it directly onto the lyophilized cake to prevent mechanical shear stress.
Swirl the vial gently until the solid cake is completely dissolved; never vortex peptide solutions, as vigorous agitation can cause aggregation or denaturation. Once reconstituted, liquid aliquots should be stored at 2°C to 8°C and utilized within 21 to 28 days. For long-term liquid assays, reconstitute without preservative and store single-use aliquots at -80°C to avoid repeated freeze-thaw cycles.
Preclinical investigation into CJC-1295 DAC spans diverse physiological domains. Because the GHRH receptor signaling path governs systemic IGF-1 production, researchers utilize this peptide to evaluate connective tissue repair models, including tendon remodeling, chondrocyte proliferation, and osteoblast activity.
In metabolic research models, sustained elevation of growth hormone alters substrate utilization by increasing lipolysis in adipose tissue and modulating glucose uptake in skeletal muscle tissue. Researchers examining age-related somatopause or cellular senescence utilize long-acting GHRH constructs to study long-term receptor desensitization risks versus sustained signaling efficacy in animal models. Further details on GHRH signaling pathways are documented in our GHRH analogs overview.
Reliable scientific outcomes require raw materials synthesized under rigid quality control standards. PX1 Research manufactures research compounds in state-of-the-art, GMP-compliant facilities within the United States. Every production lot undergoes comprehensive analytical screening, providing researchers with complete transparency.
Orders are dispatched same-day from our centralized fulfillment centers in California and Arizona (Monday through Friday). Each product page links directly to public, downloadable Certificate of Analysis (COA) documents displaying verified HPLC chromatograms and Mass Spec molecular mass verification, ensuring that experimental variables remain tightly controlled.
What does 'pharmacie grade' mean in the context of research CJC-1295 DAC?
In research settings, pharmaceutical-grade ('pharmacie') refers to peptides synthesized to extreme purity standards (>99%), verified by RP-HPLC and Mass Spectrometry, with endotoxin levels controlled below <0.01 EU/mg. It distinguishes high-purity analytical compounds from unrefined chemical grade materials.
What is the primary structural difference between CJC-1295 DAC and CJC-1295 No DAC?
CJC-1295 DAC contains a Drug Affinity Complex (maleimidopropionic acid linker) that covalently binds to circulating serum albumin, extending its biological half-life to 6-8 days in animal models. CJC-1295 No DAC lacks this linker, resulting in a short half-life of approximately 30 minutes.
How does CJC-1295 DAC stimulate growth hormone release in preclinical models?
CJC-1295 DAC binds selectively to the GHRH receptor on anterior pituitary somatotropes. This activates adenylate cyclase and the cAMP pathway, stimulating synthesis and secretagogue release of endogenous growth hormone and subsequent systemic IGF-1 elevation.
Is CJC-1295 DAC intended for human consumption or medical therapy?
No. CJC-1295 DAC supplied by PX1 Research is strictly for laboratory research, in vitro assays, and animal studies. It is not approved for human consumption, clinical use, or veterinary therapeutic application.
What documentation accompanies CJC-1295 DAC from PX1 Research?
Every lot of CJC-1295 DAC is shipped with a lot-specific Certificate of Analysis (COA) generated by an independent ISO 17025 accredited laboratory. The COA includes RP-HPLC purity profiles, mass spec identity verification, and endotoxin assay data.
How should lyophilized CJC-1295 DAC be stored upon arrival?
Unopened, lyophilized CJC-1295 DAC should be stored at -20°C or -80°C in a dry environment protected from light. Under these conditions, the peptide remains stable for up to 24 months.
What fluid is recommended for reconstituting CJC-1295 DAC for lab assays?
For routine laboratory studies requiring multiple draws over 3–4 weeks, sterile Bacteriostatic Water (0.9% benzyl alcohol) is typically utilized. For sensitive cell culture or immediate in vivo assays, sterile normal saline or sterile water without preservatives may be specified.
What are the shipping locations for PX1 Research orders?
PX1 Research ships all research compounds directly from facilities located in California and Arizona, offering same-day dispatch for orders placed Monday through Friday.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.