Cjc 1295 No Dac Test Report
A CJC 1295 No DAC test report provides essential analytical validation for biochemical and cell-culture investigations. Obtaining lot-specific purity data ensures reproducible baseline conditions when evaluating synthetic growth hormone secretagogues in laboratory models.
Quick answer
A CJC 1295 No DAC test report provides essential analytical validation for biochemical and cell-culture investigations. Obtaining lot-specific purity data ensures reproducible baseline conditions when evaluating synthetic growth hormone secretagogues in laboratory models.
Key takeaways
- A CJC 1295 No DAC test report is an official [Certificate of Analysis](/research-peptides/what-is-a-coa-for-peptides) (COA) generated by an independent, ISO 17025-accredited analytical laboratory to verify the identity, purity, and safety profile of a synthetic peptide lot.
- CJC 1295 No DAC, chemically designated as Modified GRF (1-29), is a 29-amino acid synthetic analog of natural Growth Hormone-Releasing Hormone (GHRH).
- Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) serves as the primary quantitative method for determining chemical purity in a peptide test report.
- While HPLC quantifies purity, Electrospray Ionization Mass Spectrometry (ESI-MS) verifies molecular identity by measuring the exact mass-to-charge ratio (m/z) of the peptide.
Analytical Definition of a CJC 1295 No DAC Test Report
A CJC 1295 No DAC test report is an official Certificate of Analysis (COA) generated by an independent, ISO 17025-accredited analytical laboratory to verify the identity, purity, and safety profile of a synthetic peptide lot. The document details Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) chromatograms showing >98% chemical purity, Electrospray Ionization Mass Spectrometry (ESI-MS) confirming molecular mass, and endotoxin quantification.
For investigators procuring synthetic signals from the research peptides catalog, verifying a complete analytical report before initiating in vitro assays or animal models prevents experimental artifacts caused by sequence truncations, residual solvents, or bacterial endotoxins. Every batch of CJC 1295 No DAC supplied by PX1 Research undergoes stringent batch testing to ensure complete lot traceability.
Molecular Architecture and Structure of Modified GRF (1-29)
CJC 1295 No DAC, chemically designated as Modified GRF (1-29), is a 29-amino acid synthetic analog of natural Growth Hormone-Releasing Hormone (GHRH). Its primary sequence—Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2—contains four strategic amino acid substitutions relative to native GHRH (1-29).
These targeted substitutions (D-Ala2, Gln8, Ala15, and Leu27) enhance enzymatic stability against dipeptidyl peptidase-IV (DPP-IV) cleavage while maintaining full binding affinity for the GHRH receptor. Unlike its conjugated counterpart, CJC 1295 No DAC omits the Lys(Maleimidopropionyl) Drug Affinity Complex (DAC) linker. Consequently, the peptide exhibits a biological half-life of approximately 30 minutes in preclinical models, facilitating precise, transient stimulation of pituitary somatotrophs without permanent serum albumin binding.
Interpreting RP-HPLC Purity Metrics in Test Reports
Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) serves as the primary quantitative method for determining chemical purity in a peptide test report. During RP-HPLC analysis, the sample is injected onto a non-polar stationary phase (typically a C18 column) and eluted using a hydrophobic gradient of acetonitrile and water containing trifluoroacetic acid (TFA).
Purity is expressed as a percentage calculated from the area under the curve (AUC) of the primary peptide peak relative to total integrated peaks. High-grade research material requires a primary peak area exceeding 98.0%. Minor secondary peaks indicate trace synthesis side-products, such as deletion sequences or beta-isomerized peptides. Examining the baseline stability and peak symmetry within our detailed research library guides assists laboratory personnel in interpreting analytical chromatography.
Mass Spectrometry (ESI-MS) Identity Verification
While HPLC quantifies purity, Electrospray Ionization Mass Spectrometry (ESI-MS) verifies molecular identity by measuring the exact mass-to-charge ratio (m/z) of the peptide. The theoretical monoisotopic mass of CJC 1295 No DAC (C152H252N44O42) is 3367.97 Da.
In a standard ESI-MS test report, spectra display multiple ionization states, including [M+2H]2+, [M+3H]3+, and [M+4H]4+ ions. Deconvolution software converts these charge states into a single observed molecular mass. A valid test report confirms that the observed molecular mass matches the theoretical mass within a strict tolerance window (typically ±1.0 Da), proving that the full-length 29-amino acid chain was assembled correctly without missing residues.
Endotoxin Testing and Bioburden Limits for Preclinical Use
Bacterial endotoxins—lipopolysaccharides (LPS) derived from the outer membrane of Gram-negative bacteria—pose significant risks to cellular cultures and animal studies. High endotoxin levels trigger non-specific inflammatory signaling pathways, skewing cytokine assays and invalidating physiological research results.
Quality test reports quantify endotoxins using the Limulus Amebocyte Lysate (LAL) kinetic chromogenic assay. PX1 Research enforces an endotoxin threshold of <0.01 EU/mg for all research compounds. Verifying this metric in a test report ensures that observed cellular responses are attributable solely to GHRH receptor stimulation rather than pyrogen-induced stress pathways.
Preclinical Pharmacodynamics and Research Applications
As a synthetic GHRH analog, CJC 1295 No DAC is studied as a long-acting growth-hormone-releasing hormone that sustains GH and downstream IGF-1 levels for tissue repair research. In vitro assays demonstrate that binding to GHRH receptors on anterior pituitary cells stimulates intracellular cyclic AMP (cAMP) accumulation, triggering exocytosis of growth hormone storage vesicles.
Preclinical rodent models indicate that pulsatile growth hormone secretion drives hepatic transcription of Insulin-like Growth Factor 1 (IGF-1). Researchers utilize this mechanism to investigate collagen synthesis, skeletal muscle hypertrophy, cell proliferation, and cellular repair processes. The absence of the DAC moiety allows researchers to mimic physiological GHRH pulses without causing continuous, baseline-elevated somatotroph stimulation.
Comparative Analytical Profiles: CJC 1295 No DAC vs. Class Alternatives
When designing comparative secretagogue protocols, researchers frequently evaluate CJC 1295 No DAC alongside other GHRH derivatives and ghrelin receptor agonists. Selecting the appropriate secretagogue depends on the required pharmacokinetics and pulsatility profiles.
In analytical studies, CJC-1295 DAC exhibits a extended elimination half-life (~5 to 8 days) due to covalent albumin binding, whereas CJC 1295 No DAC provides a transient ~30-minute half-life ideal for pulse-mimicking models. When combined in dual-receptor research with a GHRP such as Ipamorelin, a synergistic release of GH is observed in vitro. Comparative data detailed in our Tesamorelin vs CJC 1295 overview further illustrate how sequence modifications alter receptor binding kinetics across different GHRH analogs.
Laboratory Reconstitution and Storage Protocol Standards
To maintain the structural integrity validated in a test report, lyophilized CJC 1295 No DAC must be reconstituted using proper laboratory protocol. The standard diluent is sterile bacteriostatic water containing 0.9% benzyl alcohol, which inhibits microbial growth during multi-dose container access.
During reconstitution, the diluent should be directed down the glass vial wall rather than sprayed directly onto the peptide cake. Gentle swirl mixing is recommended; vigorous vortexing or shaking can break delicate peptide bonds and form insoluble aggregates. Reconstituted solutions should be stored at 2°C to 8°C and used within 28 days. Lyophilized vials reserved for long-term storage should be held at -20°C in a dry environment.
Verifying Supplier Quality and Lot Traceability
Evaluating a supplier's quality control standards requires inspecting the transparency and completeness of their test documentation. High-purity peptides must be synthesized in cGMP-compliant facilities and tested by third-party laboratories using ISO 17025 certified methodologies.
PX1 Research provides comprehensive, lot-specific COAs for every peptide batch. Institutional buyers managing large-scale screening protocols can utilize our wholesale lab account portal to review batch records, HPLC raw chromatograms, and mass spec analytical data prior to placing institutional orders. All orders ship directly from ISO-certified distribution hubs located in California and Arizona with same-day dispatch for orders finalized Monday through Friday.
Frequently Asked Questions
What is the primary difference between CJC 1295 No DAC and CJC 1295 with DAC?
CJC 1295 No DAC (Modified GRF 1-29) lacks the Drug Affinity Complex maleimide reactive group. As a result, it does not bind to serum albumin in vivo, exhibiting a biological half-life of approximately 30 minutes compared to the 5–8 day half-life of CJC 1295 with DAC.
What purity level should be documented in a CJC 1295 No DAC test report?
High-grade research peptides must demonstrate an RP-HPLC purity of 98.0% or greater. Secondary peaks representing synthesis impurities or truncated sequences should not exceed 2.0% of the total integrated peak area.
How is the molecular identity of CJC 1295 No DAC verified on a COA?
Molecular identity is verified using Electrospray Ionization Mass Spectrometry (ESI-MS). The spectrum must display an observed monoisotopic molecular mass that matches the theoretical target mass of 3367.97 Da within a ±1.0 Da mass tolerance window.
Why is endotoxin testing critical for CJC 1295 No DAC research lots?
Endotoxins (lipopolysaccharides) induce severe pyrogenic and inflammatory responses in cell cultures and animal models. Ensuring an endotoxin level below 0.01 EU/mg prevents false-positive inflammatory signaling during growth hormone secretagogue bioassays.
What solvent is recommended for reconstituting CJC 1295 No DAC for laboratory assays?
Lyophilized CJC 1295 No DAC is typically reconstituted using sterile bacteriostatic water (0.9% benzyl alcohol) or sterile isotonic saline, depending on the requirements of the specific cell culture or animal research protocol.
How should CJC 1295 No DAC be stored upon receipt in the laboratory?
Lyophilized peptide vials should be stored at -20°C in a desiccated environment away from light. Once reconstituted into solution, the liquid should be stored between 2°C and 8°C and evaluated within 28 days to avoid hydrolytic degradation.
Does PX1 Research provide lot-specific COAs with every CJC 1295 No DAC order?
Yes. Every batch of CJC 1295 No DAC manufactured for PX1 Research undergoes independent third-party HPLC, ESI-MS, and endotoxin analysis. Lot-specific test reports are accessible directly through our online verification portal.
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