Epithalon 50mg — Vial Spec, Reconstitution Math & COA
A 50mg vial of Epithalon provides a high-mass lyophilized footprint designed for high-throughput assays, extended longitudinal cell culture models, or multi-sample comparative study designs. This technical document details the precise reconstitution mathematics, physical specifications, handling protocols, and analytical validation metrics required for rigorous laboratory research.
Quick answer
A 50mg vial of Epithalon provides a high-mass lyophilized footprint designed for high-throughput assays, extended longitudinal cell culture models, or multi-sample comparative study designs. This technical document details the precise reconstitution mathematics, physical specifications, handling protocols, and analytical validation metrics required for rigorous laboratory research.
Key takeaways
- In analytical chemistry and cell biology research, selecting the appropriate vial mass is critical to maintaining consistency across multi-well plate assays and long-term research designs.
- [Epithalon](/research-peptides/epithalon) is a synthetic short-chain peptide composed of four amino acid residues arranged in the sequence Ala-Glu-Asp-Gly.
- Accurate reconstitution mathematics are paramount when working with high-mass vials like [epithalon](/research-peptides/epithalon) 50mg.
- Due to the substantial peptide mass contained within a single 50mg vial, working solutions should be managed using precise aliquot protocols to prevent chemical degradation.
Overview and Availability of High-Mass Epithalon Formulations
In analytical chemistry and cell biology research, selecting the appropriate vial mass is critical to maintaining consistency across multi-well plate assays and long-term research designs. A standard 50mg specification of Epithalon (Epitalon) contains 50 milligrams of the synthetic tetrapeptide (L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine) in a vacuum-sealed, lyophilized cake format. This bulk-scale vial layout is primarily selected by institutional laboratories requiring high-concentration stock solutions, high-volume cell culture dosing protocols, or automated liquid-handling workflows where frequent reconstitution of smaller units introduces inter-assay variability.
While custom 50mg configurations are frequently requested for large-scale research projects, investigators should verify current catalog options across our all-peptides selection. PX1 Research standardly supplies high-purity Epithalon in standardized 10mg and 20mg laboratory formats to minimize peptide degradation associated with repeated freeze-thaw cycles. When larger total masses are required for institutional applications, multi-vial analytical runs or wholesale procurement options ensure batch-matched consistency across all experimental runs without compromising compound integrity.
Chemical Identity, Molecular Structure, and Physicochemical Properties
Epithalon is a synthetic short-chain peptide composed of four amino acid residues arranged in the sequence Ala-Glu-Asp-Gly. Originally designed to replicate the biological activity of endogenous pineal gland peptide extracts (epithalamin), its reduced molecular weight (418.4 g/mol) allows for predictable solubility and rapid diffusion dynamics in aqueous laboratory buffers.
Preclinical studies suggest that the primary carboxyl and amino termini of this tetrapeptide interact directly with target nuclear components and chromatin structures. The exact physical stability of the synthetic molecule depends heavily on its purity profile and net neutral-to-slightly-acidic pKa characteristics in solution. To ensure exact stoichiometric ratios in chemical assays, raw materials are processed through controlled freeze-drying cycles, producing a highly porous matrix optimized for rapid hydration without aggregate formation.
Reconstitution Mathematics for a 50mg Epithalon Vial Format
Accurate reconstitution mathematics are paramount when working with high-mass vials like epithalon 50mg. Because 50 milligrams represents 50,000 micrograms (mcg) of raw active substance, small variations in diluent volume yield dramatic shifts in working solution concentration. Standard laboratory practice requires adding sterile bacteriostatic water or phosphate-buffered saline (PBS) via a slow, wall-directed stream into the vial under negative pressure.
When reconstituting a 50mg vial, researchers must account for final concentration metrics depending on the chosen volume of diluent. Adding 1.0 mL of liquid creates a working concentration of 50 mg/mL (500 mcg per 0.01 mL or 10 units on a standard U-100 volumetric scale). Introducing 2.0 mL yields a concentration of 25 mg/mL (250 mcg per 0.01 mL), whereas 3.0 mL provides a working concentration of 16.67 mg/mL (166.7 mcg per 0.01 mL). To rapidly model alternative concentrations or secondary dilution steps, investigators should reference our interactive reconstitution-calculator to eliminate manual conversion error.
Aliquot Planning, Storage Stability, and Thermal Dynamics
Due to the substantial peptide mass contained within a single 50mg vial, working solutions should be managed using precise aliquot protocols to prevent chemical degradation. Lyophilized Epithalon maintained at -20°C or -80°C exhibits stability for extended periods, but once dissolved in aqueous diluents, hydrolysis and peptide oxidation accelerate at ambient temperatures.
Following initial reconstitution, the master stock should be aliquoted into sterile, low-binding microcentrifuge tubes in volumes matched to daily assay consumption (e.g., 100 µL to 250 µL units). These primary aliquots must be flash-frozen and kept at -20°C or colder. Repeated freeze-thaw cycles degrade the tertiary structure and cleave peptide bonds, reducing assay reproducibility. Reconstituted aqueous samples stored at standard refrigeration temperatures (2°C to 8°C) should be utilized strictly within 14 to 21 days.
Bioregulator Mechanism: Telomerase Activation and Telomere Maintenance
Epithalon is categorized within the bioregulator class of peptides, which are defined by their capacity to modulate gene expression through direct or indirect interaction with chromatin. In vitro data indicate that Epithalon induces the expression of human telomerase reverse transcriptase (hTERT), the catalytic subunit of the enzyme telomerase responsible for maintaining chromosomal end-cap structures known as telomeres.
In somatic cellular senescence models, telomeres progressively shorten with each cycle of DNA replication until reaching the Hayflick limit, triggering apoptosis or irreversible cell cycle arrest. Preclinical studies suggest that exposure to synthetic Epithalon allows senescent or aging cell lines to re-express telomerase activity, thereby stabilizing telomere length and extending viable replication passage counts in controlled culture environments.
Pineal Axis Modulating and Circadian Neuroendocrine Pathways
Beyond chromosomal maintenance, research models involving rodent and non-human primate systems have highlighted Epithalon’s involvement in regulating pineal gland function and neuroendocrine secretion. The pineal gland plays a central role in modulating circadian rhythms through the cyclical synthesis of melatonin from serotonin.
In animal models of accelerated aging or pineal involution, administration of Epithalon restored night-time melatonin synthesis levels to baseline values observed in younger control groups. In vitro assays evaluating pinealocyte cultures further demonstrate that the peptide upregulates key enzymes involved in the melatonin biosynthesis pathway without destabilizing baseline hormone feedback loops.
Analytical Quality Assurance: COA Metrics and Purity Standards
Every batch of synthetic research compound supplied by PX1 Research undergoes rigorous testing in an independent, ISO 17025 accredited laboratory facility. A lot-specific Certificate of Analysis (COA) is generated for each production run, ensuring absolute transparency regarding chemical composition, identity, and cleanliness.
High-Performance Liquid Chromatography (HPLC) is employed to confirm chemical purity, with PX1 requiring a minimum purity threshold of ≥98.0%. Mass Spectrometry (MS) confirms the precise molecular weight of 418.4 Da, ensuring the absence of truncated sequences, deletion peptides, or residual protecting groups. Furthermore, every batch undergoes chromogenic Limulus Amebocyte Lysate (LAL) testing to confirm that bacterial endotoxin levels remain strictly below laboratory safety limits (<0.1 EU/mg).
Comparative Analysis: Epithalon vs. Class-Adjacent Research Peptides
When structuring experimental designs focused on cellular longevity, tissue homeostasis, or endocrine modulation, researchers frequently compare Epithalon against other bioregulator and repair peptides. A brief comparison highlights the operational distinctions among these compounds:
While Epithalon specifically targets nuclear telomerase gene expression and pineal activity, Pinealon operates as a tripeptide bioregulator localized toward central nervous system protection and synaptogenesis. Conversely, Thymalin functions primarily within immune system regulation, enhancing T-cell differentiation in thymocyte models. For systemic tissue repair and cellular migration models, scientists often implement non-bioregulator peptides like BPC-157. Understanding these distinct molecular mechanisms allows researchers to select the precise tool required for their specific cell-line or tissue model.
Vial Size Selection Strategy: 50mg vs. Standard Laboratory Mass
Choosing between a single 50mg vial configuration and smaller mass allocations (such as 10mg or 20mg units) involves balancing assay throughput against peptide stability considerations. The primary benefit of a 50mg format is operational efficiency during high-throughput screening where large volumes of reconstituted peptide are consumed within a single experimental shift.
Conversely, for studies requiring intermittent dosing over several weeks or months, deploying smaller 10mg or 20mg vials minimizes the exposure of reconstituted stock solutions to environmental ambient fluctuations and prolonged aqueous storage. Researchers evaluating long-term research strategies should consult the complete research index to determine optimal mass allocation protocols based on trial duration and reagent stability profiles.
Summary Procedures for Laboratory Reconstitution and Handling
To ensure reproducible experimental outcome data when handling epithalon 50mg or related formats, research personnel must adhere to strict aseptic protocols within a certified laminar flow hood. Sanitization of vial stoppers with 70% isopropyl alcohol prior to needle insertion prevents airborne contamination of the sterile lyophilized cake.
Reconstitution should always be performed using high-purity laboratory solvents, such as sterile water for injection or bacteriostatic water containing 0.9% benzyl alcohol to prevent microbial proliferation during multi-dose sampling. The reconstituted fluid should be gently swirled rather than vigorously vortexed to prevent shear stress on the peptide backbone, ensuring structural integrity prior to analytical measurement.
Frequently Asked Questions
What is the molecular weight and sequence of Epithalon?
Epithalon (Epitalon) has a molecular weight of 418.4 g/mol and consists of the four amino acid sequence L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine (Ala-Glu-Asp-Gly).
How much diluent is required for a 50mg Epithalon vial?
Diluent volume depends on target assay concentration. Reconstituting 50mg with 1.0 mL yields a 50 mg/mL concentration, 2.0 mL yields 25 mg/mL, and 3.0 mL yields 16.67 mg/mL. Precision calculations can be confirmed via our reconstitution calculator.
Does PX1 Research offer Epithalon in a 50mg vial size?
PX1 Research standardly stocks Epithalon in 10mg and 20mg laboratory vial sizes to ensure optimal stability and prevent repeated freeze-thaw cycles. Custom 50mg fills or high-mass bulk orders are available for specialized institutional projects through wholesale channels.
What is the primary mechanism of Epithalon studied in preclinical models?
In vitro and animal models show that Epithalon acts as a peptide bioregulator, studied primarily for its ability to induce telomerase expression (hTERT), maintain telomere length, and regulate pineal melatonin secretion.
How should reconstituted Epithalon 50mg stock solutions be stored?
Reconstituted stock should be aliquoted into low-binding tubes to avoid freeze-thaw cycles and frozen at -20°C or -80°C for extended storage. Refrigerated aqueous solutions at 2–8°C should be utilized within 14–21 days.
What testing is performed to verify the purity of Epithalon?
Every lot undergoes independent ISO 17025 laboratory testing using High-Performance Liquid Chromatography (HPLC) for purity (≥98.0%), Mass Spectrometry (MS) for sequence identification, and LAL testing for bacterial endotoxins.
Is Epithalon approved for human or therapeutic consumption?
No. Epithalon is supplied strictly as a synthesized research chemical for in vitro, cell culture, and laboratory research use only. It is not intended for clinical, medical, therapeutic, or human use.
What is the maximum endotoxin threshold for PX1 Research peptides?
All peptide lots from PX1 Research must pass chromogenic LAL assays verifying endotoxin levels are strictly below 0.1 EU/mg prior to final lot distribution.
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