PNC-27 15mg — Vial Spec, Reconstitution Math & COA
A 15mg vial of PNC-27 provides a high-mass lyophilized yield designed for high-throughput in vitro screens, cell viability assays, and multi-plate dosage titrations. Formulated for academic and institutional laboratories, this mass format minimizes lot-to-lot variance across extensive cell culture series while maintaining strict analytical purity.
Quick answer
A 15mg vial of PNC-27 provides a high-mass lyophilized yield designed for high-throughput in vitro screens, cell viability assays, and multi-plate dosage titrations. Formulated for academic and institutional laboratories, this mass format minimizes lot-to-lot variance across extensive cell culture series while maintaining strict analytical purity.
Key takeaways
- The 15mg PNC-27 vial specification contains precisely 15 milligrams of purified lyophilized peptide powder, calculated by amino acid analysis (AAA) to account for residual salt and moisture content.
- PX1 Research maintains a rigorous catalog of analytical-grade research compounds.
- Preclinical studies suggest that PNC-27 operates as a targeted, membrane-active peptide capable of inducing rapid cell lysis in transformed malignant cell lines while sparing non-transformed somatic cells.
- Reconstitution calculations for a 15mg vial require careful accounting of desired molarity or mass concentration (mg/mL) relative to the total liquid diluent volume introduced.
15mg PNC-27 Specification & Physical Properties
The 15mg PNC-27 vial specification contains precisely 15 milligrams of purified lyophilized peptide powder, calculated by amino acid analysis (AAA) to account for residual salt and moisture content. PNC-27 is a synthetic, membrane-active peptide derived from the p53 residue sequence (residues 12–26) coupled to a transmembrane-penetrating domain (penetratin). In its lyophilized state, the cake presents as a dense white to off-white cake sealed under an inert argon atmosphere within USP Type I borosilicate glass vials to prevent atmospheric oxidative degradation.
Principal investigators and laboratory managers select the 15mg fill size when conducting large-scale cell line exposure assays, microfluidic cell-killing kinetics, or multi-replicate flow cytometry studies. Utilizing a single 15mg vial reduces batch-to-batch reconstitution steps, ensuring uniform baseline molar concentrations across extended operational timelines. Each 15mg format unit supplied for laboratory research undergoes rigorous mass spectrometry and HPLC analysis to confirm target molecular weight (approximately 4,000.6 Da) and sequence fidelity.
Catalog Availability & PX1 Packaging Formats
PX1 Research maintains a rigorous catalog of analytical-grade research compounds. While high-mass 15mg custom lots are routinely processed for institutional clients requiring bulk allocations through our wholesale lab account portal, standard catalog offerings may vary between 5mg, 10mg, and specialized bulk runs. Researchers seeking immediate off-the-shelf single-vial unit orders should inspect our primary PNC-27 product page for real-time inventory updates.
If your experimental framework specifically mandates a 15mg batch size not currently displayed in the core catalog, contact our technical team to arrange custom synthesis or access pre-allocated bulk lots. You can also explore our broader catalog of cell biology reagents across all research peptides to coordinate co-treatments or comparative controls for your experimental models.
Molecular Mechanism: HDM-2 Targeting & p53-Independent Necrosis
Preclinical studies suggest that PNC-27 operates as a targeted, membrane-active peptide capable of inducing rapid cell lysis in transformed malignant cell lines while sparing non-transformed somatic cells. The peptide sequence contains an active domain derived from the p53 binding region that specifically interacts with Human Double Minute-2 (HDM-2) proteins. In transformed cells, HDM-2 is frequently overexpressed and aberrantly localized within the plasma membrane, unlike in healthy tissues where HDM-2 remains predominantly intranuclear or cytosolic.
In vitro data indicate that upon binding to membrane-bound HDM-2, PNC-27 undergoes a conformational transition, inserting its hydrophobic domain into the lipid bilayer. This interaction leads to the rapid formation of transmembrane pores—frequently termed 'membranolytic pores'—resulting in severe loss of membrane integrity, cytosolic leakage, and acute necrotic cell death. Notably, this mechanism operates entirely independent of intracellular p53 signaling or apoptotic pathways, rendering PNC-27 an important research tool for investigating p53-mutated or p53-null cancer cell models in preclinical research studies.
Precise Reconstitution Calculations for 15mg Vials
Reconstitution calculations for a 15mg vial require careful accounting of desired molarity or mass concentration (mg/mL) relative to the total liquid diluent volume introduced. Because a 15mg payload represents a higher solute mass than standard 2mg or 5mg vials, liquid volume addition must be calibrated based on the solubility limit of the peptide construct and the target working stock concentration.
To establish precise working concentrations, refer to the mathematical breakdown below for standard diluent additions to a 15mg PNC-27 lyophilized cake: • 1.0 mL Diluent Addition: Yields a stock concentration of 15.0 mg/mL (15.0 µg/µL). • 2.0 mL Diluent Addition: Yields a stock concentration of 7.5 mg/mL (7.5 µg/µL). • 3.0 mL Diluent Addition: Yields a stock concentration of 5.0 mg/mL (5.0 µg/µL). • 5.0 mL Diluent Addition: Yields a stock concentration of 3.0 mg/mL (3.0 µg/µL). For custom volume calculations or molarity conversions (mM/µM based on a molecular weight of ~4,000.6 g/mol), investigators should utilize our interactive reconstitution calculator prior to solvent introduction.
Laboratory Reconstitution Protocols & Solvent Compatibility
Reconstitution must be performed under a sterile laminar flow hood utilizing proper aseptic techniques. PNC-27 exhibits strong solubility in standard aqueous buffers, sterile bacteriostatic water, or phosphate-buffered saline (PBS, pH 7.4). For assays sensitive to benzyl alcohol preservative, sterile 0.9% sodium chloride or sterile molecular-biology-grade water should be selected.
Allow the lyophilized vial to equilibrate to room temperature (20°C to 25°C) for 30 minutes before solvent addition to prevent atmospheric moisture condensation inside the vial. Direct the stream of solvent down the inner glass wall of the vial rather than directly onto the lyophilized pellet. Gently swirl the vial in a circular motion until the solute is completely dissolved. Avoid vigorous vortexing or rapid agitation, as mechanical shear stress can induce peptide aggregation or surface denaturation.
Aliquot Management & Sub-Zero Storage Protocol
Because a 15mg yield supports multiple high-throughput experimental runs, repeated freeze-thaw cycles of the reconstituted stock solution must be strictly avoided. Repeated temperature cycling accelerates peptide degradation via peptide bond hydrolysis and hydrophobic aggregation. Following complete dissolution, immediately divide the working stock into single-use microcentrifuge aliquots using sterile, low-protein-binding polypropylene tubes.
Store reconstituted aliquots at -20°C for short-term evaluation (up to 30 days) or -80°C for long-term storage (up to 12 months). Unopened, lyophilized vials should be maintained at -20°C in a desiccated environment protected from direct light exposure. Prior to introducing an aliquot into cell culture media, thaw the unit rapidly at room temperature and brief-centrifuge to collect condensation, using the entire contents within the immediate testing block.
Analytical Quality Standards: COA, HPLC & Endotoxin Limits
Every production lot of PNC-27 manufactured for PX1 Research undergoes rigorous, independent ISO 17025 laboratory verification before distribution. We issue lot-matched Certificates of Analysis (COAs) for every fill size, confirming peptide purity, sequence verification, and safety parameter thresholds. Laboratory managers can verify their specific lot metrics at any time by accessing our public COA verification repository.
Key analytical metrics verified per lot include: • Chemical Purity: Confirmed ≥98.0% via High-Performance Liquid Chromatography (HPLC) peak area integration. • Identity Verification: Mass Spectrometry (ESI-MS or MALDI-TOF) confirming theoretical molecular weight. • Endotoxin Control: Chromogenic LAL assay confirming endotoxin levels <0.05 EU/mg, preventing non-specific immune pathway activation in sensitive in vitro cell lines. • Counterion Content: Trifluoroacetate (TFA) salt residual content quantified and reported to ensure non-cytotoxic baselines during bioassays.
Comparative Sizing: 15mg vs. 5mg and 10mg Vials
Determining whether a 15mg vial format or smaller units (such as 5mg or 10mg) are optimal depends entirely on experimental design and assay scale. A 15mg vial offers distinct economic and logistical benefits for high-throughput screening (HTS) laboratories running automated liquid handlers across multi-well plates. It eliminates intra-assay variability caused by reconstituting multiple smaller vials during a single large-scale screen.
Conversely, if experimental assays are executed sporadically or across low-density multi-well plates (e.g., 24-well or 6-well formats), multiple 5mg or 10mg vials may be preferable. Smaller unit sizes minimize the duration that reconstituted peptide remains in liquid storage, reducing the risk of accidental degradation or bacterial contamination over prolonged experimental timelines.
Peptide Class Comparisons in Membrane Dynamics and Cell Death
In cell biology and oncology research, PNC-27 is routinely categorized alongside other membrane-active and pore-forming peptides, as well as signaling regulators investigated for selective cytotoxicity. For instance, investigators examining membrane disruption mechanisms often compare PNC-27 with amphipathic lytic peptides or specialized signaling molecules such as melittin analogs and cytotoxic domain fragments.
Additionally, laboratories evaluating apoptotic pathways alongside necrotic mechanisms often cross-reference PNC-27 with p53-pathway regulators like p53-activating constructs or signaling peptides such as BPC-157 when evaluating differential tissue growth, vascular signaling, and cellular survival profiles. Understanding these structural and mechanistic distinctions allows researchers to construct robust, multi-peptide screening panels.
PX1 Supply Chain, USA Manufacturing, and Shipping Standards
PX1 Research produces all research compounds within state-of-the-art, GMP-compliant facilities located strictly in the United States. Our manufacturing protocols follow standardized solid-phase peptide synthesis (SPPS) pipelines, followed by multi-stage purification and lyophillization under Class 100 cleanroom conditions. This guarantees that academic, clinical, and biotechnology facilities receive consistent, pure compounds free from heavy metals or industrial contaminants.
Orders placed Monday through Friday ship same-day from our dual logistics hubs located in California and Arizona. Lyophilized peptides are packaged in temperature-stable insulated containers designed to buffer ambient thermal fluctuation during transit. For institutional purchasing inquiries, bulk lot reservations, or specialized synthesis, visit our dedicated wholesale procurement page.
Frequently Asked Questions
What is the exact theoretical molecular weight of PNC-27?
PNC-27 has a calculated molecular weight of approximately 4,000.6 g/mol. Exact monoisotopic and average mass values are detailed on the lot-matched HPLC/MS documentation provided with each order.
How much diluent should be added to a 15mg PNC-27 vial for a 10 mg/mL solution?
To achieve a 10 mg/mL stock concentration, precisely 1.5 mL of sterile solvent (bacteriostatic water or sterile PBS) must be added to the 15mg lyophilized cake.
Is PNC-27 soluble directly in standard PBS buffer?
Yes, PNC-27 demonstrates good solubility in standard phosphate-buffered saline (PBS, pH 7.4). Gentle circular swirling is recommended to achieve complete dissolution without causing protein aggregation.
Why is PNC-27 studied in p53-mutated or p53-null cell lines?
In vitro studies indicate that PNC-27 induces cell lysis via physical pore formation upon binding membrane-bound HDM-2. Because this mechanism acts directly on the cell membrane, it functions independently of intracellular p53 transcriptional activity.
How should reconstituted 15mg PNC-27 working stocks be stored?
Reconstituted solutions should be divided into single-use aliquots using low-binding polypropylene tubes and stored at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
What endotoxin limits are guaranteed for PX1 PNC-27 lots?
PX1 Research enforces a strict endotoxin threshold of <0.05 EU/mg, verified via chromogenic LAL testing, ensuring compatibility with sensitive cell cultures.
Does PX1 offer a 15mg vial size directly in the standard retail catalog?
Catalog availability for specific vial sizes varies. High-mass formats such as 15mg are available via custom institutional synthesis or institutional wholesale requests. Standard sizes can be checked on our primary PNC-27 product page.
Where can I download the lot-matched COA for my PNC-27 shipment?
Certificates of Analysis (COAs) detailing HPLC purity and mass spec verification can be downloaded directly from our COA portal using the lot number printed on your vial.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.