Buy VIP — USA-Made, COA per Lot
Vasoactive Intestinal Peptide (VIP) is a crucial 28-amino acid neuropeptide utilized extensively in preclinical research evaluating neuroinflammation, immunomodulation, and cellular signaling pathways. PX1 Research supplies high-purity, USA-synthesized VIP backed by lot-specific HPLC, Mass Spectrometry, and endotoxin analysis for rigorous analytical and in vitro investigation.
Quick answer
Vasoactive Intestinal Peptide (VIP) is a crucial 28-amino acid neuropeptide utilized extensively in preclinical research evaluating neuroinflammation, immunomodulation, and cellular signaling pathways. PX1 Research supplies high-purity, USA-synthesized VIP backed by lot-specific HPLC, Mass Spectrometry, and endotoxin analysis for rigorous analytical and in vitro investigation.
Key takeaways
- Vasoactive Intestinal Peptide (VIP) is a basic 28-amino acid regulatory peptide originally isolated from porcine intestinal tissue, belonging to the glucagon/secretin superfamily of signaling molecules.
- Preclinical investigations demonstrate that VIP signals primarily through two high-affinity Class B G-protein coupled receptors: VPAC1 and VPAC2.
- Research teams seeking to acquire [research peptides](/research) frequently encounter significant variance between domestic suppliers and low-cost overseas exporters.
- Bacterial endotoxins (lipopolysaccharides, or LPS) present a major confounding variable in immunological and neurochemical research.
Overview of Vasoactive Intestinal Peptide (VIP) in Research
Vasoactive Intestinal Peptide (VIP) is a basic 28-amino acid regulatory peptide originally isolated from porcine intestinal tissue, belonging to the glucagon/secretin superfamily of signaling molecules. In native biological systems, VIP functions as a potent non-adrenergic, non-cholinergic neurotransmitter and neuromodulator. It exerts diverse biological effects across the central nervous system, gastrointestinal tract, cardiovascular system, and immune apparatus.
When laboratory researchers evaluate options to buy VIP for in vitro or animal models, compound purity and structural integrity are paramount. Unrefined or impure peptide preparations introduce variable chemical artifacts that obscure cell culture assays and receptor-binding studies. PX1 Research manufactures VIP via solid-phase peptide synthesis (SPPS) in USA-based, ISO 17025 accredited facilities to ensure precise sequence accuracy, optimal counter-ion balance, and complete elimination of truncated peptide fragments.
Receptor Kinetics and Downstream Signaling Pathways
Preclinical investigations demonstrate that VIP signals primarily through two high-affinity Class B G-protein coupled receptors: VPAC1 and VPAC2. Upon ligand binding, these receptors couple to the Gs alpha subunit, activating adenylyl cyclase and triggering a downstream intracellular surge of cyclic adenosine monophosphate (cAMP). This secondary messenger cascade activates Protein Kinase A (PKA) and downstream transcription factors like CREB (cAMP response element-binding protein).
In vitro data indicate that VIP also exhibits cross-reactivity with the NPR-C (natriuretic peptide receptor-C) and low-affinity interactions with scavenger receptors under specific physiological concentrations. Because these receptor dynamics require exact ligand stoichiometry, utilizing raw materials verified by analytical chromatography is essential for reproducing established affinity constants (Kd) and functional activation metrics (EC50) in laboratory settings.
Why Sourcing Quality Matters: PX1 Research vs. Overseas Suppliers
Research teams seeking to acquire research peptides frequently encounter significant variance between domestic suppliers and low-cost overseas exporters. Overseas manufacturers often rely on high-throughput synthesis techniques that sacrifice crude purification steps. This frequently results in residual trifluoroacetic acid (TFA) salts, organic solvents, racemized amino acid side chains, and unidentified deletion peptides remaining in the lyophilized cake.
In contrast, PX1 Research applies multi-stage preparative High-Performance Liquid Chromatography (HPLC) purification to every lot. By exchanging aggressive TFA counter-ions for bio-compatible acetate or chloride salts, PX1 yields a refined research compound that reduces non-specific cellular toxicity in fragile primary cell lines and organoid cultures. Furthermore, relying on domestic inventory eliminates regulatory delays, customs seizures, and cold-chain breakdowns common to international transit.
Endotoxin Testing & HPLC/MS Purity Verification
Bacterial endotoxins (lipopolysaccharides, or LPS) present a major confounding variable in immunological and neurochemical research. Ingested or baseline endotoxin contamination can trigger toll-like receptor 4 (TLR4) activation, masking or falsifying the target biological activity of VIP in macrophages, microglia, and dendritic cell assays. Overseas suppliers rarely perform quantitative chromogenic LAL assays, leaving researchers at risk of compromised experimental data.
PX1 Research enforces strict quality control thresholds. Every batch of VIP undergoes comprehensive testing in an ISO 17025 accredited laboratory, including full spectrum Electrospray Ionization Mass Spectrometry (ESI-MS) to verify precise molecular weight (3325.8 Da) and analytical HPLC to guarantee purity exceeding 98%. Furthermore, every lot is screened for bacterial endotoxin content, ensuring levels remain below stringent institutional limits (<0.01 EU/mg). Researchers can review our transparency protocol and endotoxin testing guidelines directly within our technical hub.
Preclinical Research Applications: Immunomodulation & Neuroprotection
In preclinical animal models and cell culture frameworks, VIP is actively studied for its capacity to suppress pro-inflammatory cytokine production. In vitro assays using lipopolysaccharide-stimulated murine macrophages indicate that VIP administration downregulates nuclear factor kappa B (NF-kB) translocation, leading to reduced expression of TNF-alpha, IL-6, IL-12, and inducible nitric oxide synthase (iNOS).
In neurobiological research, VIP exhibits marked neuroprotective effects against excitotoxic insult and oxidative stress. Rodent models of neurodegeneration suggest that VIP promotes neuronal survival by stimulating astroglial secretion of activity-dependent neuroprotective protein (ADNP) and neurotrophic factors. Researchers investigating central nervous system homeostasis frequently pair VIP with other signaling peptides to map complex paracrine networks.
Comparative Analysis: VIP vs. Related Regulatory Peptides
When designing comparative bioassays, investigators often compare VIP against structurally or functionally related regulatory compounds within the glucagon/secretin peptide family. For instance, PACAP-38 shares approximately 68% sequence homology with VIP and binds VPAC1 and VPAC2 receptors, but uniquely displays nanomolar affinity for the PAC1 receptor. Similarly, secretin acts on the secretin receptor to modulate exocrine pancreatic secretion and central fluid balance, demonstrating distinct downstream physiological effects.
To explore immunomodulatory mechanisms broader than GPCR-mediated cAMP pathways, laboratory teams often evaluate VIP alongside antimicrobial or thymic peptides such as Thymosin Alpha-1 and LL-37. While VIP primarily functions via anti-inflammatory pathway suppression, peptides like Thymosin Alpha-1 act to enhance T-cell maturation and immune system baseline vigilance, providing complementary experimental axes in preclinical immunology studies.
Technical Specifications & Reconstitution Protocols for Laboratory Use
PX1 Research supplies VIP as a sterile, lyophilized white powder packaged in sealed, climate-controlled glass vials. Reconstitution must be carried out under sterile laminar flow conditions using appropriate laboratory reagents. For short-term enzymatic assays, sterile bacteriostatic water or phosphate-buffered saline (PBS, pH 7.4) is typically used to dissolve the peptide cake.
Due to the basic nature of the 28-amino acid sequence, complete dissolution occurs rapidly upon mild vortexing. To preserve peptide stability, researchers should avoid vigorous mechanical agitation, which can induce physical shear stress and lead to surface aggregation. For precise molarity calculations during assay setup, research staff can utilize our interactive laboratory reconstitution calculator.
Storage, Stability, and Handling Requirements
Lyophilized VIP remains stable for up to 24 months when stored in a desiccated environment at -20°C or -80°C. Upon receiving the product, laboratory personnel should briefly centrifuge the vial to pellet any loose material prior to removing the stopper.
Once reconstituted into aqueous solution, VIP should be aliquoted into single-use microcentrifuge tubes and stored at -80°C to prevent degradation caused by repeated freeze-thaw cycles. Working solutions kept at 4°C should be utilized within 3 to 5 days to ensure maximum biological potency and guard against opportunistic microbial growth.
Procurement Options: Bulk Sourcing and Laboratory Accounts
To support multi-phase preclinical trials, screening initiatives, and institutional research projects, PX1 Research provides flexible supply options ranging from individual analytical vials to bulk custom synthesis. Academic laboratories, biotechnology firms, and contract research organizations (CROs) can streamline procurement through our dedicated wholesale portal.
Institutional accounts receive dedicated account management, customized batch sizes, batch reservation options, and extended analytical validation. Every order, regardless of volume, includes complete lot-traceable Documentation of Analysis (COA) detailing raw chromatographic and mass spectroscopic data.
Why PX1 Research is the Premier US Source for Academic Labs
PX1 Research operates exclusively as an in-house manufacturer and distributor of high-tier laboratory research reagents. By maintaining complete oversight of synthesis, purification, analytical testing, and cold-chain packaging within USA-based facilities, we eliminate the supply chain vulnerabilities and quality defects common with secondary re-sellers.
All orders placed Monday through Friday are processed and dispatched same-day from our strategically located fulfillment facilities in California and Arizona. Fast domestic transit ensures that delicate compounds like VIP arrive at your facility with minimal thermal exposure, ready for immediate integration into your scientific workflows.
Frequently Asked Questions
What is the purity level of PX1 Research VIP?
Every lot of VIP supplied by PX1 Research is verified via analytical High-Performance Liquid Chromatography (HPLC) to guarantee a chemical purity exceeding 98%.
Does PX1 provide a Certificate of Analysis (COA) with each order?
Yes. Every single lot of VIP is accompanied by a batch-specific COA containing full HPLC chromatograms, Electrospray Ionization Mass Spectrometry (ESI-MS) spectra, and quantitative LAL endotoxin test results.
Where is PX1 VIP synthesized and shipped from?
PX1 Research VIP is synthesized in cGMP-compliant, ISO 17025 accredited facilities in the United States and shipped directly from our primary distribution hubs in California and Arizona.
What are the endotoxin limits for VIP research compounds?
PX1 Research enforces strict endotoxin controls, certifying that all research-grade VIP contains less than 0.01 EU/mg as measured by chromogenic LAL testing, making it safe for sensitive cell culture and in vitro models.
How should VIP be reconstituted in a laboratory setting?
Reconstitution should occur under a sterile biosafety cabinet using sterile bacteriostatic water or PBS (pH 7.4). Swirl gently to dissolve; avoid violent shaking or sonication to prevent protein denaturing.
How long does shipping take within the United States?
Orders placed before cutoff times Monday through Friday ship same-day from CA or AZ via expedited carrier service, typically arriving at US research facilities within 1 to 3 business days.
Can VIP be ordered in bulk for institutional research?
Yes. Academic departments, CROs, and industrial biotech facilities can request custom multi-gram batches or establish recurring shipments via the PX1 wholesale account portal.
Is VIP intended for human consumption or therapeutic use?
No. VIP provided by PX1 Research is strictly designated as a research chemical for in vitro, cell culture, and laboratory animal research use only. It is not intended for human or veterinary medical applications.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.