Buy FOXO4-DRI — USA-Made, COA per Lot
FOXO4-DRI (Forkhead box O4 D-retro-inverso) is a specialized synthetic peptide engineered to disrupt the p53-FOXO4 interaction in cellular senescence models. PX1 Research supplies high-purity, USA-synthesized FOXO4-DRI verified via HPLC and mass spectrometry to support demanding laboratory investigations. Every production lot is independently analyzed for sequence identity, chemical purity, and residual endotoxin levels.
Quick answer
FOXO4-DRI (Forkhead box O4 D-retro-inverso) is a specialized synthetic peptide engineered to disrupt the p53-FOXO4 interaction in cellular senescence models. PX1 Research supplies high-purity, USA-synthesized FOXO4-DRI verified via HPLC and mass spectrometry to support demanding laboratory investigations. Every production lot is independently analyzed for sequence identity, chemical purity, and residual endotoxin levels.
Key takeaways
- Cellular senescence represents a state of stable cell-cycle arrest triggered by various cellular stresses, including DNA damage, oxidative stress, and oncogene activation.
- FOXO4-DRI is a 36-amino-acid peptide derived from the interaction domain of the FOXO4 protein.
- When procurement officers and principal investigators decide to purchase reagents, selecting a qualified vendor is crucial for data reproducible across assay runs.
- To understand the technical advantages of domestic sourcing, research facilities can evaluate the key operational differences between typical overseas drop-shippers and PX1 Research:
Introduction to FOXO4-DRI in Cellular Senescence Research
Cellular senescence represents a state of stable cell-cycle arrest triggered by various cellular stresses, including DNA damage, oxidative stress, and oncogene activation. In preclinical cell culture and animal models, senescent cells express distinctive markers such as p16INK4a and senescence-associated beta-galactosidase (SA-β-gal), while secreting a proinflammatory cocktail known as the senescence-associated secretory phenotype (SASP).
Investigating targeted interventions that induce apoptosis specifically in senescent cell populations without harming non-senescent somatic cells is a major focus of modern biogerontology. Researchers seeking to buy FOXO4-DRI rely on its unique molecular architecture to selectively disrupt nuclear retention signals in senescent cells. By competing with endogenous FOXO4 for binding to p53, FOXO4-DRI encourages the exclusion of p53 from the nucleus or liberates p53 to initiate mitochondrial apoptotic cascades in cell line assays.
Molecular Architecture: The D-Retro-Inverso Design
FOXO4-DRI is a 36-amino-acid peptide derived from the interaction domain of the FOXO4 protein. Standard L-amino acid peptides are highly susceptible to rapid degradation by endogenous serum and cellular proteases, limiting their utility in extended in vitro culture studies and preclinical animal models.
To overcome enzymatic cleavage, FOXO4-DRI utilizes a D-retro-inverso configuration. This synthetic modification involves two key structural changes: reversing the sequence of amino acids (retro) and substituting naturally occurring L-amino acids with their D-enantiomers (inverso). The resulting topological structure presents side chains in an orientation analogous to the native parent peptide while establishing complete resistance to conventional peptidases. Laboratory investigators evaluating research peptides utilize this structural modification to maintain experimental stability over prolonged incubation windows.
Sourcing Considerations: PX1 Research vs. Overseas Suppliers
When procurement officers and principal investigators decide to purchase reagents, selecting a qualified vendor is crucial for data reproducible across assay runs. Foreign suppliers frequently offer lower upfront costs, but overseas peptide manufacturing often introduces critical vulnerabilities: inconsistent purity, lack of lot-specific analytical data, residual trifluoroacetic acid (TFA), and unquantified endotoxin contamination.
PX1 Research enforces strict quality control standards that eliminate vendor-induced variability. All peptides are synthesized domestically in cGMP-compliant facilities, processed under automated solid-phase peptide synthesis (SPPS) protocols, and purified via preparative reverse-phase HPLC. Unlike imported materials accompanied by generic, template-driven documentation, every batch of PX1 material is validated by an accredited ISO 17025 partner laboratory.
Comparative Vendor Specifications for Preclinical Reagents
To understand the technical advantages of domestic sourcing, research facilities can evaluate the key operational differences between typical overseas drop-shippers and PX1 Research:
• Synthesis Location: Overseas sources typically utilize mass-scale offshore facilities; PX1 Research manufactures exclusively within the USA under GMP-compliant operational frameworks. • Analytical Validation: Overseas vendors often provide static, representative COAs; PX1 provides authentic, lot-specific HPLC and ESI-MS reports generated by third-party laboratories. • Endotoxin Screening: Overseas materials are rarely tested for bacterial lipopolysaccharides; PX1 conducts chromogenic LAL assays on every batch to guarantee levels below strict research thresholds. • Counter-Ion Content: Offshore peptide salts frequently contain excess TFA that disrupts cell viability; PX1 utilizes standardized desalting protocols to minimize TFA toxicity in culture. • Shipping Speed: Overseas transit introduces custom clearance delays and thermal fluctuation risks; PX1 offers same-day dispatch (Monday through Friday) from dual distribution hubs in California and Arizona.
Preclinical Mechanisms: Disruption of p53-FOXO4 Binding
In vitro data indicate that under normal physiological conditions, p53 coordinates cell-cycle arrest, repair mechanisms, or programmed cell death. In senescent cells, however, FOXO4 expression is upregulated, sequestering p53 within the nucleus and preventing p53-mediated apoptosis. This sequestration allows senescent cells to survive indefinitely while maintaining high SASP output.
FOXO4-DRI acts as a competitive antagonist against the p53 binding pocket on endogenous FOXO4. In preclinical models, application of FOXO4-DRI leads to the release of p53 from the complex. Unbound p53 can then translocate to the mitochondria, triggering cytochrome c release, caspase-3 and caspase-9 activation, and rapid apoptosis specifically in senescent cells. Crucially, non-senescent cells—which maintain lower basal FOXO4 expression—exhibit minimal sensitivity to FOXO4-DRI exposure in cell culture experiments.
Comparative Analysis: FOXO4-DRI and Related Longevity Research Compounds
When designing protocols for cellular aging, researchers frequently compare FOXO4-DRI with other cellular targets and mitochondrial manipulators. While FOXO4-DRI operates as a targeted senolytic peptide by interfering with p53 nuclear retention, compounds such as Epithalon focus on telomerase induction and chromatin structure modulation.
Similarly, mitochondrial-targeted peptides like SS-31 interact directly with cardiolipin to reduce electron transport chain ROS leakage, whereas MOTS-c serves as a mitochondrial-derived signaling molecule modulating metabolic homeostasis under cellular stress. Combining targeted senolytics with mitochondrial protectants allows investigators to evaluate multi-target strategies in comprehensive aging studies. Exploring broader research pathways is accessible through the complete PX1 Research catalog.
Stringent Quality Assurance: HPLC/MS Verification and Lot-Specific COAs
Data integrity in quantitative assays relies on exact reagent purity. Impurities in synthetic peptide preparations—such as truncated sequences, deletion peptides, or residual solvents—can introduce unintended biochemical confounding factors or off-target cell death.
Every batch of material supplied when you buy FOXO4-DRI from PX1 Research undergoes rigorous High-Performance Liquid Chromatography (HPLC) to verify chemical purity above 98%. Electrospray Ionization Mass Spectrometry (ESI-MS) confirms precise molecular weight matching the calculated monoisotopic mass of the D-retro-inverso sequence. Each shipment includes a verifiable, lot-specific Certificate of Analysis detailing these results.
Endotoxin Quantification and Batch Consistency
Bacterial endotoxins (lipopolysaccharides) are potent pyrogens that trigger innate immune responses in cell cultures and animal tissue models, causing non-specific cell death and invalidating experimental readouts. Many commercial vendors bypass endotoxin testing due to added analytical overhead.
PX1 Research subjects every lot of research peptides to kinetic chromogenic Limulus Amebocyte Lysate (LAL) testing. We verify that residual endotoxin levels remain strictly below <0.01 EU/μg. This rigorous threshold prevents artifactual cytokine release and non-specific toxicity during sensitive in vitro assays and cell-viability screens.
Reconstitution, Handling, and Storage Protocols for In Vitro Assays
To preserve the bioactivity of FOXO4-DRI, proper laboratory handling procedures must be followed upon receipt. Lyophilized peptides should be stored at -20°C or -80°C for long-term stability, protected from light and moisture.
When preparing stock solutions for laboratory investigation, bring the vial to room temperature prior to reconstitution to minimize condensation. FOXO4-DRI is typically reconstituted in sterile, cell-culture-grade water or phosphate-buffered saline (PBS). For difficult-to-dissolve concentrations, gentle sonication or the addition of a small percentage of dimethyl sulfoxide (DMSO) may be employed. Reconstituted stock solutions should be aliquoted into single-use microcentrifuge tubes to prevent repeated freeze-thaw cycles, which degrade peptide bonds. Review our detailed peptide reconstitution guide for step-by-step laboratory calculations.
Fulfillment Efficiency: Same-Day Domestic Dispatch
Research timelines require dependable supply chain schedules. Overseas shipping often subjects climate-sensitive biological reagents to extended customs holds, temperature spikes, and unreliable delivery tracking.
PX1 Research operates strategic distribution facilities in California and Arizona. Orders placed before 3:00 PM EST Monday through Friday are processed and dispatched the same day. All shipments utilize protective insulated packaging with temperature-control elements to maintain structural integrity throughout transit, guaranteeing that laboratory materials arrive in pristine condition.
Bulk Acquisition and Institutional Accounts
For academic institutions, contract research organizations (CROs), and high-throughput laboratory screening operations requiring large quantities of target peptides, PX1 Research offers flexible sourcing solutions. Large-scale projects require batch consistency across multi-gram quantities to maintain inter-assay reproducibility.
Through our dedicated wholesale program, institutional clients receive prioritized batch allocation, custom lyophilization formats, and dedicated account management. Contact our technical team to discuss custom synthesis runs, specific counter-ion exchanges (such as acetate conversion), or multi-lot reservation agreements.
Frequently Asked Questions
What is FOXO4-DRI and how is it used in laboratory research?
FOXO4-DRI is a synthetic 36-amino-acid D-retro-inverso peptide designed to interfere with p53-FOXO4 binding in cellular models. It is supplied exclusively as a research-grade reagent for in vitro assays and preclinical cellular senescence studies.
What is the purity level of FOXO4-DRI from PX1 Research?
Every lot of FOXO4-DRI supplied by PX1 Research achieves a purity of ≥98% as determined by reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Electrospray Ionization Mass Spectrometry (ESI-MS).
How does PX1 Research test for endotoxins in FOXO4-DRI batches?
PX1 Research utilizes kinetic chromogenic Limulus Amebocyte Lysate (LAL) assays to quantify endotoxin levels. Every batch is certified to contain <0.01 EU/μg of endotoxin to prevent non-specific cellular toxicity.
Why is the D-retro-inverso structure significant for research protocols?
The D-retro-inverso sequence incorporates D-amino acids in a reversed sequence orientation. This structure preserves the biological binding topology of the parent peptide while rendering the molecule highly resistant to enzymatic cleavage in culture.
What solvent is recommended for reconstituting FOXO4-DRI in the lab?
FOXO4-DRI is typically reconstituted using sterile, cell-culture-grade water or sterile phosphate-buffered saline (PBS, pH 7.4). For high-concentration stock solutions, low percentages of DMSO may be utilized.
How should lyophilized and reconstituted FOXO4-DRI be stored?
Lyophilized FOXO4-DRI should be stored at -20°C or -80°C. Once reconstituted, stock solutions should be aliquoted into single-use vials and stored at -80°C to minimize freeze-thaw degradation.
How fast does PX1 Research ship orders containing FOXO4-DRI?
PX1 Research offers same-day shipping for orders placed before 3:00 PM EST, Monday through Friday. Orders ship directly from fulfillment facilities located in California and Arizona.
Can institutions purchase FOXO4-DRI in bulk quantities?
Yes, PX1 Research provides institutional accounts and bulk purchasing programs with custom lot sizes, batch reservation, and specialized analytical documentation for qualified laboratories.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.