Written by PX1 Research Team
PX1 chemists and research educators with hands-on experience in US-based peptide manufacturing, HPLC / mass-spectrometry lot testing, and endotoxin QC. All content is citation-backed and peer-reviewed for accuracy.
Klotho Protein Research Guide: alphaKlothoLR Explained (2026)
Research Guide
Reviewed By
PX1 QC — Analytical Chemistry Team
Every article is reviewed by PX1's in-house analytical team for accuracy on mechanism, dosing ranges reported in the literature, and lab-handling guidance. We do not publish clinical or medical advice.
Quick answer
Klotho research guide: what α-Klotho is, its FGF23 co-receptor role, the long-release alphaKlothoLR construct, handling, and verified Klotho research vials.
Key takeaways
- Klotho — specifically α-Klotho — is a protein researched for its role as a co-receptor for FGF23 signaling.
- What is alphaKlothoLR?
- Handling Klotho protein
- Sourcing Klotho from PX1 Research
Klotho — specifically α-Klotho — is a protein researched for its role as a co-receptor for FGF23 signaling. This guide explains what Klotho is, how the long-release research construct alphaKlothoLR differs from native Klotho, and how to handle it in the lab.
What is Klotho?
α-Klotho is a transmembrane and soluble protein studied extensively since its discovery in 1997. In laboratory research it is best known as the co-receptor that allows FGF23 to bind its receptors, linking it to phosphate and mineral signaling pathways.
What is alphaKlothoLR?
alphaKlothoLR is a long-release recombinant α-Klotho construct for in vitro research. It joins the 1012-amino-acid α-Klotho protein to a 29-amino-acid albumin-binding peptide through a glycine-serine linker.
| Property | Value |
|---|---|
| Construct | α-Klotho (1012 aa) + albumin-binding peptide |
| Molecular weight | ~133–135 kDa |
| Albumin binding (Kd) | <20 nM |
| FGF23 binding (Kd) | 15–30 nM |
| Reported cellular activity | Up to 19 days in laboratory models |
| PX1 format | Two 10mcg vials per order |
Handling Klotho protein
Klotho is a large protein, so gentle handling matters more than with short peptides. Reconstitute slowly, swirl rather than shake, avoid repeated freeze-thaw cycles and keep refrigerated after reconstitution.
Sourcing Klotho from PX1 Research
Each PX1 alphaKlothoLR order includes two 10mcg vials, verified by HPLC and LC-MS. View Klotho (alphaKlothoLR) at PX1 Research.
For laboratory research use only. Not for human consumption.
Frequently asked questions
What is Klotho? α-Klotho is a protein studied as the co-receptor for FGF23 signaling.
Is Klotho a peptide? It is a large protein (~1,012 amino acids); alphaKlothoLR adds a short albumin-binding peptide.
How many vials come with an order? Each order includes two 10mcg vials.
How should Klotho be handled? Reconstitute gently, avoid shaking and freeze-thaw cycles, and refrigerate.