PX1 Research serves as a premier USA-based supplier of high-purity 5-Amino-1MQ for analytical testing, cell culture assays, and preclinical research applications. Every lot undergoes rigorous third-party verification to ensure structural identity, exact purity, and compliance with stringent laboratory standards.
PX1 Research serves as a premier USA-based supplier of high-purity 5-Amino-1MQ for analytical testing, cell culture assays, and preclinical research applications. Every lot undergoes rigorous third-party verification to ensure structural identity, exact purity, and compliance with stringent laboratory standards.
A reliable 5-amino-1mq supplier must provide analytical verification of purity, identity, and safety through batch-specific documentation. PX1 Research delivers laboratory-grade 5-Amino-1MQ manufactured in US-based GMP-compliant facilities, verified by independent ISO 17025 accredited laboratories via High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) to guarantee chemical integrity for preclinical investigations.
When sourcing small molecules and specialized enzymes or inhibitors for metabolic assays, researchers must evaluate supplier quality beyond basic marketing claims. Inferior synthesis, residual solvents, or heavy metal contamination can invalidate sensitive cell culture models and generate false positive or negative outcomes in enzyme kinetics assays. Evaluating supplier standards—including transparent access to lot-matched Certificates of Analysis (COAs)—is crucial for reproducible scientific inquiry.
Investigators acquiring the 5-Amino-1MQ research compound through PX1 Research receive analytical documentation verifying active compound concentration and confirming low endotoxin levels suitable for rigorous downstream applications.
5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective, membrane-permeable small molecule inhibitor of nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide (NAM), producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH).
By inhibiting NNMT activity, 5-Amino-1MQ prevents the irreversible methylation and subsequent renal clearance of nicotinamide. Because nicotinamide serves as a direct precursor to nicotinamide adenine dinucleotide (NAD+), NNMT inhibition effectively preserves cellular pools of salvageable NAM. Preclinical models indicate that inhibiting this enzymatic bottleneck allows cells to maintain elevated intracellular NAD+ concentrations without requiring exogenous NAD+ supplementation.
Additionally, because NNMT consumes SAM as a methyl donor, excessive NNMT activity alters cellular methylation potential by lowering the SAM/SAH ratio. In vitro assays demonstrate that treating high-NNMT-expressing cell lines with 5-Amino-1MQ restores intracellular SAM levels, potentially influencing global histone methylation patterns and epigenomic regulation in metabolic tissues.
Preclinical literature demonstrates that 5-Amino-1MQ plays a significant role in modulating cellular metabolic pathways. In rodent models of diet-induced obesity, administration of NNMT inhibitors resulted in reduced adipocyte volume, enhanced systemic energy expenditure, and marked improvements in lipid clearance mechanisms. These effects occurred independent of food intake modifications, suggesting direct cellular action on metabolic rate.
At the cellular level, studied mechanisms center around raising NAD+ levels and improving mitochondrial output. In vitro data indicate that elevated NAD+ concentrations drive increased activity of sirtuin enzymes (specifically SIRT1 and SIRT3), which regulate mitochondrial biogenesis and oxidative phosphorylation. Researchers exploring cellular respiration parameters often combine 5-Amino-1MQ with other metabolic research compounds to evaluate synergistic impacts on oxygen consumption rates (OCR) and extracellular acidification rates (ECAR).
Furthermore, fat-metabolism research highlights 5-Amino-1MQ as a key molecule for studying adipocyte differentiation and lipid accumulation. By modulating the NNMT/1-MNA pathway in mature adipocytes, researchers can investigate how cellular methyl donor availability influences lipolysis, fatty acid beta-oxidation, and insulin signaling pathways in vitro.
When evaluating a 5-amino-1mq supplier, stringent testing methodologies are required to ensure that experimental parameters remain unconfounded by chemical impurities. Superior research suppliers execute comprehensive testing protocols across every batch of synthesized raw materials.
Primary identity and purity verifications are conducted using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) coupled with Mass Spectrometry (MS). RP-HPLC quantifies the relative abundance of 5-Amino-1MQ relative to synthesis byproducts, establishing a purity profile of ≥98%. Electrospray Ionization Mass Spectrometry (ESI-MS) confirms the exact molecular mass (209.11 g/mol for the free ion) and chemical structure, ruling out regioisomers or incomplete reaction intermediates.
Endotoxin testing via Limulus Amebocyte Lysate (LAL) assays is an equally vital quality control standard. Endotoxins, which are lipopolysaccharide (LPS) contaminants derived from gram-negative bacterial cell walls, induce profound inflammatory signaling in cell cultures and animal models. PX1 Research mandates third-party endotoxin testing for all lot releases, ensuring that concentrations remain well below established analytical thresholds for preclinical research.
To contextualize the scientific utility of 5-Amino-1MQ, researchers frequently compare its mechanism of action with distinct peptides and metabolic agents that influence mitochondrial function or lipid homeostasis. Reviewing compounds across our complete catalog of research peptides provides structural context for experimental design.
While 5-Amino-1MQ acts upstream as a small molecule enzyme inhibitor to preserve NAD+ pools, mitochondrial-derived peptides like MOTS-c peptide operate via nuclear translocation to regulate folate metabolism and AMPK activation. Conversely, lipolytic fragments such as AOD-9604 research compound act through specific adipose receptor pathways to stimulate lipolysis without altering cellular methyl-donor availability.
Researchers investigating systemic bioenergetics often cross-reference 5-Amino-1MQ with direct NAD+ boosters and mitochondrial regulators. Detailed scientific breakdowns of these distinct pathways are available in our cellular energy research peptides reference guide, helping laboratories select the optimal target molecule for their specific study protocols.
Proper handling and solubilization procedures are vital to maintain the molecular stability and biological activity of 5-Amino-1MQ in laboratory settings. 5-Amino-1MQ is typically supplied as a lyophilized salt (e.g., iodide or bromide salt) requiring precision reconstitution.
For cell culture assays, 5-Amino-1MQ exhibits high solubility in polar organic solvents such as dimethyl sulfoxide (DMSO) and sterile water, depending on the specific salt form. Researchers preparing stock solutions should utilize sterile, filter-sterilized DMSO to create concentrated stocks (e.g., 10 mM to 50 mM). Concentrated stock solutions can then be diluted directly into working cell culture media immediately prior to application, ensuring that final vehicle concentrations (such as DMSO) remain below cytotoxic thresholds (typically <0.1% v/v).
It is recommended to aliquot stock solutions into single-use, low-binding microcentrifuge tubes to prevent degradation caused by repeated freeze-thaw cycles. Detailed assay design frameworks and solvent compatibility parameters are archived within our accessible research library.
Maintaining chemical stability requires strict adherence to temperature and environmental controls. In its solid, lyophilized state, 5-Amino-1MQ should be stored in a desiccated environment at -20°C for long-term storage, protected from light and ambient humidity.
Once reconstituted into aqueous or DMSO stock solutions, the compound displays reduced long-term stability relative to its dry powder state. Aliquoted liquid stock solutions are stable at -80°C for extended periods, whereas storage at 4°C is only recommended for short-term use (less than 48 hours). Laboratory personnel should inspect reconstituted solutions prior to assay addition; any visible precipitation or discoloration indicates potential compound aggregation or degradation.
PX1 Research ships all compounds in sealed, inert atmosphere packaging designed to prevent moisture absorption during transit. Every shipment originates from our centralized California or Arizona fulfillment hubs, supporting rapid delivery to maintain product stability.
Procuring research compounds requires full visibility into manufacturing origin, chain of custody, and analytical validation. PX1 Research manufactures all compounds within high-specification US facilities adhering to strict quality control systems.
Every batch of 5-Amino-1MQ is cataloged with a unique lot number that corresponds directly to an accessible, independent Certificate of Analysis. This document transparently outlines RP-HPLC chromatograms, mass spectra, residual solvent analysis, and endotoxin values. Laboratories seeking large-quantity procurement for extended research projects can request dedicated batch reservation through our bulk lab account infrastructure.
Orders placed Monday through Friday ship same-day from our facility network, ensuring minimal transit times and standardizing supply chain reliability for academic, clinical, and institutional research laboratories worldwide.
What is 5-Amino-1MQ studied for in laboratory settings?
5-Amino-1MQ is studied primarily as a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Preclinical research focuses on its role in raising NAD+ levels, improving mitochondrial output, regulating cellular energy balance, and supporting fat-metabolism research.
How does 5-Amino-1MQ differ from direct NAD+ precursors?
Direct NAD+ precursors (like NMN or NR) supply substrate for the salvage pathway, whereas 5-Amino-1MQ acts as an enzyme inhibitor. By blocking NNMT, 5-Amino-1MQ prevents the breakdown and methylation of existing nicotinamide, thereby conserving endogenous pools of NAD+ and maintaining SAM levels within the cell.
What purity level is guaranteed for PX1 Research 5-Amino-1MQ?
PX1 Research guarantees a minimum purity of ≥98% for 5-Amino-1MQ, verified by independent, third-party RP-HPLC and Mass Spectrometry testing. A lot-matched Certificate of Analysis (COA) is provided with every purchase.
Is 5-Amino-1MQ soluble in water or DMSO?
5-Amino-1MQ is soluble in dimethyl sulfoxide (DMSO) and readily dissolves in aqueous buffers depending on the salt formulation. For in vitro cell assays, preparing concentrated stock solutions in DMSO followed by dilution into working media is standard laboratory practice.
What are the recommended storage conditions for 5-Amino-1MQ powder?
Lyophilized 5-Amino-1MQ powder should be stored at -20°C in a dry, dark place sealed against moisture. Under these conditions, the un-reconstituted compound maintains chemical stability over extended storage periods.
Are PX1 Research compounds tested for endotoxins?
Yes. All research compounds, including 5-Amino-1MQ, undergo third-party Limulus Amebocyte Lysate (LAL) testing to ensure endotoxin levels meet strict laboratory thresholds suitable for in vitro and preclinical research applications.
Can 5-Amino-1MQ be used in clinical human studies?
No. 5-Amino-1MQ provided by PX1 Research is strictly designated for laboratory research use only, including in vitro assays and preclinical animal models. It is not intended for human consumption, therapeutic, or clinical use.
Where are PX1 Research compounds manufactured and shipped from?
All PX1 Research compounds are manufactured in US-based facilities and dispatched directly from distribution hubs located in California and Arizona. Orders placed Monday through Friday qualify for same-day fulfillment.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.