Alpha Klotho LR is a high-purity recombinant peptide variant utilized in preclinical metabolic, renal, and anti-aging research models. PX1 Research supplies analytical-grade Alpha Klotho LR with verified USA manufacturing, third-party COAs featuring HPLC/MS and endotoxin testing per lot, and immediate same-day dispatch M–F from California and Arizona.
Alpha Klotho LR is a high-purity recombinant peptide variant utilized in preclinical metabolic, renal, and anti-aging research models. PX1 Research supplies analytical-grade Alpha Klotho LR with verified USA manufacturing, third-party COAs featuring HPLC/MS and endotoxin testing per lot, and immediate same-day dispatch M–F from California and Arizona.
Alpha Klotho LR represents a synthetic or recombinant form of the single-pass transmembrane protein Alpha Klotho, engineered specifically for high stability and receptor-binding affinity in laboratory research settings. In biological systems, full-length Klotho acts as an essential co-receptor for fibroblast growth factor 23 (FGF23), regulating phosphate homeostasis, vitamin D metabolism, and cellular senescence pathways.
Researchers seeking to study soluble Klotho signaling frequently utilize recombinant Alpha Klotho variants to investigate tissue protection, oxidative stress mitigation, and longevity-associated signaling cascades in vitro and in vivo animal models. When evaluating experimental material, verifying exact molecular weight, sequence fidelity, and endotoxin levels is essential for reproducible research data.
Investigators can buy analytical-grade Alpha Klotho LR directly from PX1 Research to ensure precise sequence accuracy and complete documentation across every experimental run.
The Klotho gene family was originally identified in mutant mice exhibiting phenotypes resembling premature human aging, including vascular calcification, osteopenia, and shortened lifespan. Alpha Klotho exists in both a membrane-bound form and a soluble circulating form generated via enzymatic cleavage of the extracellular domain by membrane-bound metalloproteinases (ADAM10 and ADAM17).
Alpha Klotho LR is engineered to maintain functional enzymatic and co-receptor binding activity while offering enhanced structural stability in aqueous buffer environments. Preclinical models indicate that soluble Alpha Klotho functions as a humoral factor, interacting with multiple cell-surface receptors independent of FGF23 to modulate insulin-like growth factor 1 (IGF-1) signaling, Wnt signaling pathways, and endogenously produced reactive oxygen species (ROS).
In cell culture assays and organoid models, researchers study how soluble Alpha Klotho modulates stem cell pluripotency, cellular senescence markers (such as p16INK4a and p21), and mitochondrial bioenergetics. Detailed biochemical assays can be found within the PX1 peptide research library.
Structurally, Alpha Klotho contains two internal repeats designated KL1 and KL2, which exhibit family homology to family 1 glycosidases. Unlike typical glycosidases, soluble Alpha Klotho exhibits weak or modified catalytic activity while maintaining high-affinity binding interfaces for growth factors and cell-surface ion channels, including TRPV5 and ROMK1.
When administered in rodent models of acute kidney injury (AKI) or diabetic nephropathy, Alpha Klotho LR interacts with membrane lipids and surface proteins to alter ion channel trafficking and downregulate pro-inflammatory cytokine expression. The 'LR' designation typically identifies specific sequence modifications or long-range soluble domain constructs engineered to preserve half-life in physiological salt solutions.
To explore complementary longevity signaling peptides, researchers frequently compare Alpha Klotho data alongside studies involving FOXO4-DRI peptides and mitochondrial-targeted compounds like SS-31 peptide.
Understanding the degradation kinetics and half-life of Alpha Klotho LR is critical when designing dosing schedules for animal models or time-course incubations in cell culture experiments. In murine plasma models, endogenous soluble Klotho exhibits a relatively brief circulating half-life ranging between 20 minutes and 2 hours due to rapid renal clearance and proteolysis by circulating peptidases.
In contrast, recombinant Alpha Klotho LR constructs exhibit extended terminal elimination half-lives in vivo, often measured between 4 and 12 hours depending on the host species, vehicle composition, and stabilization modifiers. In vitro incubation assays in culture media containing 10% fetal bovine serum (FBS) demonstrate structural integrity for up to 24–48 hours at 37°C when handled under sterile conditions.
Thermal degradation studies indicate that lyophilized powder remains stable at room temperature for brief transit periods, but long-term laboratory storage requires -20°C or -80°C conditions. Repeated freeze-thaw cycles significantly reduce functional activity through protein aggregation and domain unfolding. Researchers requiring batch consistency for long-duration studies can review our full inventory across the complete peptide catalog.
Because complex proteins and peptides like Alpha Klotho LR require precise folding and purity to maintain binding kinetics, institutional laboratories must rigorously audit supplier quality metrics prior to ordering. Substandard synthesis or improper purification can introduce truncation artifacts, endotoxins, or inactive aggregates that invalidate cell culture and animal data.
The table below outlines the core quality parameters required for publishing-grade Alpha Klotho LR research:
• Purity Verification: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) showing ≥98.0% main peak purity. • Mass Verification: Electrospray Ionization Mass Spectrometry (ESI-MS) confirming precise molecular weight. • Endotoxin Control: Chromogenic LAL assay confirming <0.01 EU/µg to protect delicate cell lines and prevent immune reactions in vivo. • Domestic Sourcing: Verified synthesis and quality control inside USA facilities subject to stringent analytical oversight. • Lot Traceability: Dedicated COAs matched directly to the individual vial lot number on the shipment. • Transit Integrity: Temperature-monitored packaging with expedited dispatch from regional fulfillment hubs.
Navigating the commercial research reagent market requires vigilance against low-quality vendors. Common red flags include sellers offering protein reagents without lot-specific chromatograms, vendors using generic 'certificates of analysis' that lack mass spectra, or vendors failing to quantify endotoxin units.
Another major issue is structural degradation caused by improper shipping and storage. Vendors who drop-ship unbuffered recombinant proteins overseas at ambient temperatures risk supplying partially denatured material. Furthermore, vendors making non-compliant human health or therapeutic claims should be avoided entirely by academic and commercial laboratories prioritizing regulatory compliance.
PX1 Research maintains complete analytical transparency for every batch. Institutional teams looking to order 10 mg vials of Alpha Klotho LR receive verified COAs with every shipment.
Proper reconstitution and handling protocols are vital to preserving the tertiary structure and bioactivity of Alpha Klotho LR. Lyophilized vials should be equilibrated to room temperature before opening to prevent condensation inside the container.
Reconstitution guidelines for analytical work: 1. Reconstitute in sterile phosphate-buffered saline (PBS, pH 7.4) or sterile deionized water containing 0.1% bovine serum albumin (BSA) or human serum albumin (HSA) as a carrier protein. 2. Gently swirl or invert the vial. Never vortex vigorously, as mechanical shear stress denatures complex peptide chains. 3. Prepare single-use aliquots to avoid repeated freeze-thaw cycles. 4. Store reconstituted liquid aliquots at -80°C for long-term storage or 4°C for immediate use within 48 hours.
For comparative studies analyzing telomere maintenance or cellular senescence pathways, research teams often evaluate Alpha Klotho alongside bioregulators such as Epitalon powder or vascular integrity agents such as GHK-Cu peptide.
In anti-aging and metabolic research, Alpha Klotho LR occupies a distinct niche targeting FGF23 co-receptor dynamics, renal protection, and mineral homeostasis. However, researchers frequently pair or contrast Klotho mechanisms with other investigational agents.
While Alpha Klotho LR regulates systemic humoral signaling and oxidative defense, compounds like FOXO4-DRI specifically target senescent cell apoptosis by disrupting p53-FOXO4 binding interfaces. Similarly, mitochondrial peptides like SS-31 target cardiolipin in the inner mitochondrial membrane to reduce electron leakage, whereas Epitalon influences telomerase expression.
Evaluating these distinct pathways in parallel allows investigators to map multi-system longevity markers across cellular, tissue, and whole-organism models.
High-throughput screening assays, multi-cohort animal trials, and longitudinal research programs require substantial quantities of analytical-grade material with strict lot-to-lot uniformity. Changes in peptide purity between experimental blocks introduce unwanted noise and confound statistical analysis.
PX1 Research supports high-volume academic and enterprise research programs with dedicated batch production, custom aliquot sizing, and reserved lot locking. Laboratory directors can coordinate bulk requirements through our dedicated wholesale peptide program to lock in consistent analytical standards across extended multi-year trials.
When purchasing research compounds for high-precision assays, fast delivery and uncompromised quality control are essential. PX1 Research ships analytical-grade Alpha Klotho LR in heavy-wall glass vials featuring crimp-sealed rubber stoppers to protect lyophilized stability during transit.
All orders placed before our daily cutoff time ship same-day M–F directly from our California or Arizona distribution facilities via tracked domestic shipping services. Every single vial is backed by a lot-matched Certificate of Analysis detailing HPLC purity, ESI-MS mass verification, and LAL endotoxin levels accessible directly from our platform.
For questions regarding batch availability, technical documentation, or custom orders, our scientific support team responds promptly to clear procurement bottlenecks. Visit our main portal to buy Alpha Klotho LR for research and maintain project velocity.
What is the half-life of Alpha Klotho LR in research models?
In preclinical in vivo animal models, recombinant Alpha Klotho LR exhibits a circulating terminal half-life typically ranging from 4 to 12 hours, compared to endogenous soluble Klotho which clears within 20 to 120 minutes. In vitro stability in buffered culture media with carrier protein extends up to 24–48 hours at 37°C.
What is alpha klotho lr studied for in laboratory settings?
Alpha Klotho LR is studied primarily in preclinical models investigating phosphate homeostasis, FGF23 co-receptor signaling, cellular senescence, acute kidney injury, cardiovascular calcification, and mitochondrial oxidative stress mitigation.
Is a Certificate of Analysis provided with Alpha Klotho LR?
Yes. Every lot of Alpha Klotho LR supplied by PX1 Research includes a dedicated Certificate of Analysis (COA) containing lot-specific RP-HPLC chromatograms, mass spectrometry data, and LAL endotoxin testing results.
How fast does PX1 Research ship Alpha Klotho LR orders?
Orders placed before our same-day cutoff time Monday through Friday ship that same day from our California or Arizona fulfillment centers via tracked domestic shipping options.
How should lyophilized Alpha Klotho LR be stored upon delivery?
Lyophilized Alpha Klotho LR should be stored at -20°C or -80°C for long-term stability. While stable at ambient temperature during short domestic transit times, keeping the powder frozen upon arrival preserves functional integrity.
What buffer should be used to reconstitute Alpha Klotho LR?
Alpha Klotho LR should be reconstituted in sterile phosphate-buffered saline (PBS, pH 7.4) or sterile water containing 0.1% BSA or HSA as a carrier protein to prevent non-specific surface binding to glass or plastic containers.
Can Alpha Klotho LR withstand repeated freeze-thaw cycles?
No. Repeated freeze-thaw cycles cause protein aggregation and denaturation. Reconstituted Alpha Klotho LR should be divided into single-use aliquots and stored frozen at -80°C.
Is Alpha Klotho LR approved for human administration?
No. Alpha Klotho LR is strictly manufactured and sold for in vitro laboratory research and preclinical animal studies. It is not for human or veterinary medical use, therapy, or diagnosis.
What purity level is guaranteed for Alpha Klotho LR from PX1 Research?
PX1 Research guarantees a minimum analytical purity of ≥98.0% for Alpha Klotho LR as determined by high-performance liquid chromatography (HPLC).
How does Alpha Klotho LR differ from wild-type endogenous Klotho?
Alpha Klotho LR contains engineered sequence adjustments or soluble domain modifications optimized to enhance recombinant expression yields, buffer stability, and receptor binding affinity compared to unbuffered wild-type cleavage fragments.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.