Epitalon (Epithalon) is a synthetic tetrapeptide bioregulator studied extensively for its role in telomerase activation, pineal gland function, and cellular senescence models. PX1 Research provides analytical-grade Epitalon synthesized in GMP-compliant USA facilities, verified by lot-specific mass spectrometry and HPLC analysis. Designed strictly for in vitro assays and preclinical laboratory research, every batch undergoes rigorous endotoxin and purity testing.
Epitalon (Epithalon) is a synthetic tetrapeptide bioregulator studied extensively for its role in telomerase activation, pineal gland function, and cellular senescence models. PX1 Research provides analytical-grade Epitalon synthesized in GMP-compliant USA facilities, verified by lot-specific mass spectrometry and HPLC analysis. Designed strictly for in vitro assays and preclinical laboratory research, every batch undergoes rigorous endotoxin and purity testing.
Laboratories seeking to buy epitalon for preclinical research require analytical-grade compounds with verified identity and high purity. Epitalon (also known as Epithalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) supplied as a lyophilized powder. PX1 Research offers USA-manufactured Epitalon verified by third-party HPLC and mass spectrometry for consistent in vitro and animal model experimentation.
When institutions choose to buy epithalon online, securing valid analytical documentation is critical to experimental reproducibility. Low-quality or unverified peptides can introduce unwanted chemical contaminants, such as residual trifluoroacetic acid (TFA) salts, synthesis side-products, or bacterial endotoxins, which invalidate cellular assays. PX1 Research enforces strict quality assurance protocols, providing transparent, lot-specific documentation for every order shipped directly from our California and Arizona fulfillment centers.
Epitalon is a synthetic short-chain peptide bioregulator comprising four amino acid residues: L-alanyl-L-glutamyl-L-asparagyl-glycine (H-Ala-Glu-Asp-Gly-OH). With a molecular formula of C16H26N4O9 and a molecular mass of approximately 418.4 g/mol, this short peptide was originally modeled after Epithalamin, a natural pineal gland peptide extract isolated in early neuroendocrine studies. Researchers investigating peptide bioregulators utilize Epitalon due to its high chemical stability, low molecular weight, and specific nuclear interaction dynamics.
In physiological buffers, the tetrapeptide exhibits distinct zwitterionic behavior governed by the carboxylic acid side chains of the glutamic and aspartic acid residues. Because small regulatory peptides can undergo conformational shifts dependent on pH and ionic strength, maintaining precise buffer conditions during in vitro assays is paramount for evaluating chromatin binding, gene expression changes, and cell-surface interaction profiles.
Primary scientific interest in Epitalon centers on its demonstrated capacity to activate human telomerase reverse transcriptase (hTERT) gene expression. Preclinical studies suggest that Epitalon interacts directly with nuclear chromatin structures, inducing promoter region decondensation and upregulating endogenous telomerase activity. In somatic cell cultures, which normally undergo progressive telomeric attrition during successive cell divisions, exposure to Epitalon has been observed to preserve telomere length and extend cellular passage capacity.
Further cellular research indicates that telomerase reactivation induced by Epitalon occurs without triggering malignant transformation or dysregulated cell proliferation, a critical parameter evaluated within telomerase activators research. In vitro assays employing human somatic fibroblasts demonstrate that Epitalon treatment promotes telomere elongation by facilitating the synthesis of TTAGGG tandem repeats at terminal chromosomal ends.
Beyond its nuclear and telomeric mechanisms, Epitalon acts as a neuroendocrine bioregulator targeting the pineal-hypothalamic axis. Preclinical rodent models demonstrate that administration of Epitalon stimulates the synthesis and nocturnal secretion of endogenous melatonin by modulating N-acetyltransferase (NAT) enzymatic activity. Aged laboratory models frequently display pineal involution and blunted circadian melatonin peaks; Epitalon research indicates a potential capacity to normalize these secretory rhythms.
Investigators studying circadian biology and neuroendocrine signaling utilize research peptides like Epitalon to observe down-stream alterations in pituitary hormone release, stress-axis reactivity, and clock gene expressions (such as CLOCK and BMAL1). These dual capabilities establish Epitalon as a unique dual-action research compound affecting both genomic stability and neuroendocrine output.
Cellular senescence is accelerated by persistent reactive oxygen species (ROS), DNA double-strand breaks, and telomere shortening. In vitro data indicate that Epitalon enhances endogenous antioxidant enzyme pathways, increasing the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase. By dampening intracellular ROS concentration, Epitalon suppresses oxidative DNA damage, measured by reduced accumulation of 8-hydroxy-2'-deoxyguanosine (8-OHdG).
Experimental protocols examining the senescence-associated secretory phenotype (SASP) reveal that Epitalon exposure reduces the secretion of pro-inflammatory cytokines, including IL-6 and TNF-alpha, in senescent cell lines. Consequently, laboratories evaluating cellular longevity pathways frequently examine Epitalon in combination with targeted senolytics or mitochondrial stabilizers to map overlapping survival networks.
When evaluating longevity and cellular repair mechanisms in comparative models, investigators frequently analyze Epitalon alongside other specialized peptides. While Epitalon acts primarily as an hTERT gene activator and pineal bioregulator, mitochondrial-derived peptides like MOTS-c regulate metabolic homeostasis, skeletal muscle glucose uptake, and AMPK signaling. Similarly, Humanin provides cytoprotective effects against stress-induced apoptosis via mitochondrial pathway regulation, contrasting with Epitalon's direct nuclear chromatin interaction.
Additionally, targeted senolytic peptides such as FoxO4-DRI operate by selectively inducing apoptosis in senescent cells via disruption of the FOXO4-p53 complex, whereas Epitalon functions upstream to delay entry into senescence by preserving telomere structural integrity. In broader tissue maintenance research, scientists may also compare Epitalon's systemic genomic effects against matrix-remodeling signals like GHK-Cu or cytoprotective bioregulators like BPC-157.
To ensure maximum chemical integrity and reproducibility, Epitalon is supplied by PX1 Research as a lyophilized (freeze-dried) powder. Upon receipt, sealed vials containing dry peptide powder should be stored at -20°C for short-term projects or -80°C for long-term storage, protected from light and moisture. Lyophilized Epitalon exhibits high thermal stability during ambient transport from our California and Arizona logistics hubs, but immediate cold storage upon arrival is recommended.
Reconstitution should be conducted within a certified laminar flow hood under aseptic conditions. Depending on experimental requirements, sterile bacteriostatic water (0.9% benzyl alcohol) or sterile phosphate-buffered saline (PBS, pH 7.4) may be utilized as the solvent. Gentle hand rotation of the vial is advised to dissolve the cake; aggressive vortexing or sonic agitation must be avoided to prevent peptide denaturing. Following reconstitution, liquid aliquots should be frozen at -20°C or -80°C to eliminate repeated freeze-thaw cycles.
Scientific validation requires strict batch consistency and rigorous purity verifications. PX1 Research subjects every synthesized lot of Epitalon to rigorous third-party analytical testing. High-Performance Liquid Chromatography (RP-HPLC) is performed to confirm chemical purity, ensuring every lot meets or exceeds our standard threshold of ≥99.0% purity. Chromatographic profiles showing sharp, isolated peak resolution are published directly in lot-specific Certificates of Analysis (COAs).
Mass identity is verified independently through Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) or electrospray ionization mass spectrometry (ESI-MS), confirming the exact monoisotopic mass of 418.4 Da. Additionally, Limulus Amebocyte Lysate (LAL) testing is conducted on every batch to guarantee endotoxin levels remain below <0.01 EU/mg, preventing endotoxin-induced cellular toxicity in sensitive in vitro systems.
Academic institutions, biotechnology organizations, and independent research laboratories requiring consistent supplies of high-purity bioregulators can utilize PX1 Research's wholesale lab accounts. Our infrastructure is engineered to handle volume scaling while maintaining batch-to-batch uniformity and stringent analytical documentation.
All orders ship directly from ISO 17025-affiliated and GMP-compliant production operations located in California and Arizona. With same-day shipping on orders placed Monday through Friday before cut-off times, PX1 Research eliminates workflow interruptions for ongoing preclinical studies.
Where can institutional laboratories buy epitalon with verified purity?
Institutional laboratories can purchase Epitalon directly from PX1 Research. Every batch is manufactured in USA-based, GMP-compliant facilities and accompanied by independent ISO 17025 third-party Certificates of Analysis (COAs) verifying ≥99% purity by RP-HPLC and mass spectrometry.
How do I buy epithalon online for in vitro research?
Researchers can buy epithalon online through the PX1 Research digital portal. Orders are restricted to qualified academic, corporate, or independent laboratory accounts for preclinical and in vitro research use only.
What is the molecular sequence of Epitalon (Epithalon)?
Epitalon is a synthetic tetrapeptide with the amino acid sequence L-alanyl-L-glutamyl-L-asparagyl-glycine (Ala-Glu-Asp-Gly or AEDG), with a molecular weight of 418.4 g/mol.
What mechanism links Epitalon to telomerase activation in preclinical studies?
Preclinical studies demonstrate that Epitalon induces decondensation of nuclear heterochromatin and upregulates human telomerase reverse transcriptase (hTERT) gene expression, promoting telomere elongation in somatic cells.
How should Epitalon be reconstituted for laboratory assays?
Lyophilized Epitalon should be reconstituted under aseptic conditions using sterile bacteriostatic water or sterile PBS (pH 7.4). Swirl gently to dissolve; do not vortex aggressively.
What are the recommended storage temperature parameters for lyophilized Epitalon?
Lyophilized Epitalon should be stored at -20°C for short-term preservation or -80°C for long-term storage, protected from exposure to direct light and humidity.
How does Epitalon compare to MOTS-c or FoxO4-DRI in senescence assays?
Epitalon acts nuclear-wise on hTERT activation to preserve telomere length, whereas MOTS-c regulates mitochondrial-metabolic signaling (AMPK), and FoxO4-DRI selectively triggers apoptosis in established senescent cells.
What analytical methods verify Epitalon batch purity at PX1 Research?
PX1 Research verifies Epitalon batch purity using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) for purity assessment and Mass Spectrometry (MS) for exact molecular weight verification.
What is the endotoxin threshold for PX1 Research Epitalon lots?
All Epitalon batches supplied by PX1 Research undergo LAL endotoxin testing to ensure levels remain below <0.01 EU/mg, preventing pyrogenic or inflammatory interference in cellular models.
Can academic institutions purchase Epitalon in bulk under institutional purchase orders?
Yes, academic and industrial entities can set up verified accounts through our wholesale portal to process purchase orders, bulk quantity requests, and scheduled recurring lab deliveries.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.