An Epithalon COA (Certificate of Analysis) provides definitive third-party analytical documentation confirming the identity, purity, and quality of synthetic Epithalon. This comprehensive reference guide outlines how laboratory researchers evaluate high-performance liquid chromatography (HPLC), mass spectrometry (MS), and endotoxin testing metrics for batch-level compliance.
An Epithalon COA (Certificate of Analysis) provides definitive third-party analytical documentation confirming the identity, purity, and quality of synthetic Epithalon. This comprehensive reference guide outlines how laboratory researchers evaluate high-performance liquid chromatography (HPLC), mass spectrometry (MS), and endotoxin testing metrics for batch-level compliance.
An epithalon coa is an official third-party analytical document that verifies the chemical identity, structural integrity, purity percentage, and endotoxin threshold of a specific lot of synthetic Epithalon. It serves as the primary benchmark for researchers evaluating short-chain bioregulators for in vitro or preclinical experimental models.
Issued by accredited independent laboratories utilizing ISO 17025 standards, a reliable Certificate of Analysis guarantees that the peptide sequence (Ala-Glu-Asp-Gly) matches theoretical specifications without structural degradation, counter-ion contamination, or residual heavy metal impurities.
Epithalon (also known as Epitalon) is a synthetic tetrapeptide comprised of L-alanine, L-glutamic acid, L-aspartic acid, and glycine (H-Ala-Glu-Asp-Gly-OH). Originally derived from studies on bovine pineal gland extracts, this synthetic bioregulator is primarily investigated for its role in cellular aging, telomere maintenance, and neuroendocrine signaling.
Preclinical models demonstrate that Epithalon interacts with chromatin structures to influence gene expression profiles. In vitro assays suggest that the compound induces telomerase activity in human somatic cells, promoting telomere elongation and extending cellular proliferative capacity. Furthermore, animal studies indicate potential roles in modulating melatonin secretion, suppressing opportunistic tumor development, and regulating antioxidant enzyme activity such as superoxide dismutase (SOD) and glutathione peroxidase.
To maintain rigorous reproducibility in telomerase activation and circadian rhythm research, investigators require verified reference materials. Consultation of comprehensive analytical documentation via the PX1 research library ensures that experimental variance is minimized across longitudinal studies.
Evaluating an Epithalon COA requires methodical verification of several analytical parameters. Laboratory procurement teams should carefully audit each document against standard quality control benchmarks to ensure compound integrity prior to reconstituting lyophylized vials.
Key testing parameters that must appear on every valid COA include:
1. Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Establishes chemical purity. High-grade research Epithalon must demonstrate a primary peak purity of ≥98.0%, with minor synthesis side-products or deleted sequences strictly quantified. 2. Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms exact molecular identity. The observed monoisotopic mass must match the theoretical molecular weight of 390.35 g/mol within standard mass tolerance ranges. 3. Bacterial Endotoxin Testing (LAL Assay): Measures lipopolysaccharide contamination. Research-grade compounds intended for sensitive cell cultures should exhibit endotoxin limits below 0.1 EU/mg. 4. Appearance and Solubility: Verifies physical form (typically a white to off-white lyophilized powder) and rapid solubility in standard aqueous buffers.
Chromatographic purity assessment via RP-HPLC involves passing dissolved Epithalon through a stationary non-polar C18 column under a controlled mobile phase gradient (typically water/acetonitrile with 0.1% trifluoroacetic acid). The resulting chromatogram measures UV absorbance at 214 nm or 220 nm. The area under the curve (AUC) for the main peak relative to total integrated peak area yields the net purity percentage.
Mass spectrometry analysis complements HPLC by verifying structural composition. Using ESI-MS in positive or negative ionization mode, the instrument detects the protonated or deprotonated molecular ion species ([M+H]+ or [M-H]-). For Epithalon, a dominant mass peak at m/z 391.35 ([M+H]+) confirms the correct primary amino acid assembly without unexpected amino acid substitutions or incomplete coupling steps during solid-phase peptide synthesis (SPPS).
Bacterial endotoxins—lipopolysaccharides (LPS) derived from the outer membrane of Gram-negative bacteria—pose a significant confounding variable in cell culture experiments. High endotoxin levels can trigger unspecific inflammatory cascades, alter receptor binding assays, and cause premature cell death in delicate primary culture lines.
Quality-focused suppliers utilize the Limulus Amebocyte Lysate (LAL) chromogenic or turbidimetric assay to quantify endotoxin units per milligram (EU/mg). Standard research requirements mandate endotoxin levels <0.1 EU/mg. Every batch of synthetic material available across our complete catalog of research peptides undergoes rigorous lot-specific endotoxin screening to protect experimental integrity.
Synthetic short-chain peptides are frequently grouped by their organ-specific targets, biological origin, or structural class. When designing comparative studies on pineal function, immune modulation, or tissue regeneration, researchers often evaluate Epithalon alongside other prominent bioregulatory peptides.
For instance, while Epithalon targets pineal chromatin expression and telomerase activation, Thymalin is a synthetic thymic peptide studied for T-cell differentiation and immune system modulation. Similarly, Pinealon is a tripeptide (Glu-Asp-Arg) investigated for neuroprotective properties and brain-derived neurotrophic factor regulation. In broader cellular protection models, investigators frequently pair bioregulator studies with systemic cytoprotective agents like BPC-157 to evaluate synergistic anti-inflammatory or tissue-repair pathways. Reviewing detailed research peptides documentation helps refine dual-compound experimental protocols.
Lyophilized Epithalon is highly stable when stored in airtight, desiccant-protected containers at -20°C or -80°C. To prevent moisture condensation upon opening, allow sealed vials to equilibrate to ambient room temperature prior to opening.
For reconstitution, use sterile, laboratory-grade diluents such as sterile water for injection or bacteriostatic water depending on the intended experimental timeframe. Gently swirl or invert the vial until the lyophilized cake is fully dissolved; avoid vigorous vortexing, which can introduce shear forces that alter peptide stability. Reconstituted aliquots should be stored at 2°C to 8°C for short-term use or frozen at -20°C in single-use volumes to prevent repeated freeze-thaw cycles. Detailed volumetric calculations can be streamlined using our peptide reconstitution calculator.
PX1 Research maintains rigorous quality control frameworks tailored to institutional laboratories, university faculties, and biotechnology firms. All synthetic compounds are manufactured in domestic, GMP-compliant facilities adhering to strict US quality standards.
Every production lot is subjected to independent third-party analytical verification in an ISO 17025 accredited laboratory. Researchers receive direct access to lot-specific HPLC chromatograms, ESI-MS spectra, and LAL endotoxin test results. Orders are fulfilled with same-day dispatch (Monday through Friday) operating directly from distribution hubs located in California and Arizona. Qualified principal investigators and procurement officers seeking large-scale custom synthesis or volume discounts can establish bulk laboratory accounts for streamlined logistics.
epithalon coa
An Epithalon COA (Certificate of Analysis) is a third-party quality assurance document confirming the chemical purity (typically ≥98.0% by RP-HPLC), monoisotopic mass identity (via ESI-MS), and endotoxin limits of a specific lot of synthetic Epithalon research peptide.
How do I verify the HPLC purity on an Epithalon COA?
Review the RP-HPLC chromatogram included in the COA. Look at the primary integrated peak area at 214 nm or 220 nm relative to total peak area. The main peak area percentage represents the chemical purity of Epithalon.
What is the theoretical molecular weight of Epithalon on a Mass Spectrometry report?
The theoretical monoisotopic molecular weight of Epithalon (Ala-Glu-Asp-Gly, C14H22N4O9) is 390.35 g/mol. On an ESI-MS report, the observed protonated mass peak ([M+H]+) should appear at approximately 391.35 m/z.
What are acceptable endotoxin limits for Epithalon in laboratory research?
For cell culture and in vitro bioassays, acceptable bacterial endotoxin thresholds are strictly below 0.1 EU/mg as determined by LAL chromogenic testing.
Where can I find third-party COAs for PX1 Research peptides?
Lot-specific COAs containing full HPLC chromatograms and MS spectra are published directly on the compound product page or accessible via our dedicated COA lookup portal.
What physical appearance should lyophilized Epithalon exhibit?
High-purity Epithalon presents as a uniform, white to off-white lyophilized powder or compact cake free of visible particulate matter or discoloration.
What diluent is recommended for reconstituting Epithalon for cell culture assays?
Sterile phosphate-buffered saline (PBS, pH 7.4), sterile water, or bacteriostatic water are standard choices depending on the buffer conditions required for specific in vitro assays.
How should reconstituted Epithalon aliquots be stored?
Reconstituted liquid solutions should be divided into single-use aliquots and stored at -20°C or -80°C to minimize degradation and avoid repeated freeze-thaw cycles.
Are PX1 Research peptides manufactured in the USA?
Yes. All PX1 Research peptides are manufactured in domestic, GMP-compliant facilities and tested by independent ISO 17025 accredited laboratories in the United States.
What are the shipping options and origin hubs for Epithalon orders?
Orders are dispatched same-day Monday through Friday from our centralized warehouse facilities located in California and Arizona.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.