Buy Humanin — USA-Made, COA per Lot
PX1 Research provides institutional laboratories and academic facilities with verified, high-purity Humanin for non-clinical research. Synthesized in the USA under strict quality control standards, every batch undergoes comprehensive lot-specific analytical validation.
Quick answer
PX1 Research provides institutional laboratories and academic facilities with verified, high-purity Humanin for non-clinical research. Synthesized in the USA under strict quality control standards, every batch undergoes comprehensive lot-specific analytical validation.
Key takeaways
- When principal investigators and procurement officers evaluate options to buy Humanin for laboratory experimentation, the origin and verification methodology of the peptide are paramount.
- Humanin is a naturally occurring 24-amino acid microprotein encoded within the mitochondrial 16S ribosomal RNA gene (MTRNR2).
- Preclinical investigations into Humanin have centered heavily on its endogenous role as a stress-response factor.
- Assuring high fidelity in [cytoprotective peptide signaling](/research-peptides/cytoprotective-peptides) research requires verified chemical identity and baseline purity.
Sourcing Research-Grade Humanin: USA Synthesis vs. Overseas Vendors
When principal investigators and procurement officers evaluate options to buy Humanin for laboratory experimentation, the origin and verification methodology of the peptide are paramount. Overseas suppliers frequently distribute research compounds that suffer from batch-to-batch inconsistency, residual trifluoroacetic acid (TFA) salts, and undisclosed sequence truncated impurities. Furthermore, many overseas distributors rely on static, generic Certificates of Analysis (COAs) that are reused across multiple synthesis runs, leaving researchers without true visibility into the material purity of their specific order.
PX1 Research operates exclusively within a domestic manufacturing framework. All batch production takes place in GMP-compliant facilities located in the United States, adhering strictly to ISO 9001 and ISO 17025 quality management systems. By maintaining local control over solid-phase peptide synthesis (SPPS), purification, and lyophilization, PX1 eliminates the risks associated with international transit degradation, customs clearance delays, and dubious analytical claims. Researchers who source through our Humanin product page receive direct access to fully traceable material designed for demanding in vitro and preclinical models.
Chemical Structure and Molecular Target Profile of Humanin
Humanin is a naturally occurring 24-amino acid microprotein encoded within the mitochondrial 16S ribosomal RNA gene (MTRNR2). Identified as the founding member of the class of mitochondrial-derived peptides, Humanin possesses the primary sequence Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala (MAPRGFSCLLLLTSEIDLPVKRRA) with a molecular weight of approximately 2687.2 Da.
In cell-free and cell-culture assays, Humanin exhibits complex receptor interaction profiles. It acts via both intracellular and extracellular signaling cascades. Extracellularly, Humanin functions as a ligand for a heterotrimeric membrane receptor complex consisting of the ciliary neurotrophic factor receptor (CNTFR), the interleukin-27 receptor subunit alpha (WSX-1), and glycoprotein 130 (gp130). Additionally, studies suggest interaction with the formyl peptide receptor-like 1 (FPRL1). Intracellularly, Humanin directly binds pro-apoptotic proteins such as BAX (Bcl-2-associated X protein) and tBID, preventing their translocation to the outer mitochondrial membrane and subsequent induction of apoptosis.
Preclinical Research Findings: Cytoprotection and Metabolic Homeostasis
Preclinical investigations into Humanin have centered heavily on its endogenous role as a stress-response factor. In vitro neurobiology models utilizing primary cortical neurons and cell lines have demonstrated that Humanin administration counteracts amyloid-beta-induced neurotoxicity, oxidative stress, and excitotoxic insults. In these assays, Humanin antagonizes apoptotic signaling cascades by preserving mitochondrial membrane potential (ΔΨm) and suppressing caspase-3 cleavage.
In metabolic research, animal models indicate that Humanin and its synthetic analogs modulate insulin sensitivity and glucose homeostasis. Rodent studies evaluating systemic or central administration of Humanin observed activation of hypothalamic STAT3 (signal transducer and activator of transcription 3) signaling, leading to suppressed hepatic glucose production. Furthermore, investigators studying vascular endothelial cell cultures note that Humanin promotes nitric oxide (NO) expression and attenuates reactive oxygen species (ROS) accumulation under high-glucose conditions, highlighting its broad utility in models of metabolic dysfunction.
Analytical Quality Assurance: HPLC, MS, and ISO 17025 COA Standards
Assuring high fidelity in cytoprotective peptide signaling research requires verified chemical identity and baseline purity. PX1 Research submits every single lot of Humanin to an independent, accredited ISO 17025 testing facility. Purity is validated using High-Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry (MS) to confirm both the exact molecular weight and the absence of sequence deletion fragments or modified side-chain contaminants.
Every shipment includes a lot-specific Certificate of Analysis. The COA provides full transparency, displaying the actual chromatogram, mass spectra, net peptide content, mass balance, and residual solvent quantification. By providing accessible HPLC and MS purity analysis documentation online for every batch, PX1 eliminates guesswork, ensuring that experimental variables remain restricted to the biological parameters under study rather than peptide impurities.
Comparative Analysis: Humanin, MOTS-c, and SS-31
When designing protocols to investigate mitochondrial physiology, bioenergetics, and cellular stress responses, researchers frequently compare Humanin against other key regulatory peptides within the same research domain. Understanding the mechanistic distinctions between these targets is critical for proper assay selection.
Humanin functions primarily as an anti-apoptotic, cytoprotective factor operating through both surface receptor complexes (CNTFR/WSX-1/gp130) and direct intracellular binding to pro-apoptotic BAX proteins. In contrast, MOTS-c is a 16-amino acid mitochondrial-derived peptide that targets nuclear gene expression to regulate folate synthesis, purine metabolism, and systemic metabolic homeostasis. Meanwhile, SS-31 (Elamipretide) is a synthetic tetrapeptide that targets the inner mitochondrial membrane specifically by binding to cardiolipin, optimizing electron transport chain efficiency and reducing ROS generation independent of gene expression pathways. While all three peptides serve as powerful tools for studying mitochondrial dysfunction, Humanin remains uniquely suited for assays focused on cell survival signaling and apoptosis inhibition.
Endotoxin Risk Mitigation in Cell Culture and In Vitro Assays
Bacterial endotoxins (lipopolysaccharides, or LPS) present a profound confounding factor in immunological, neural, and metabolic research. In cellular assays, even low-nanogram concentrations of endotoxin can trigger Toll-like receptor 4 (TLR4) activation, inducing background inflammatory cytokine release (such as TNF-α, IL-1β, and IL-6) that skews research outcomes.
While low-cost commercial peptide vendors frequently forgo endotoxin testing, PX1 Research subjects all production lots to quantitative Limulus Amebocyte Lysate (LAL) assay testing. Our humanin research batches are verified to contain endotoxin levels well below industry thresholds (<0.01 EU/mg). This rigorous control guarantees that observed physiological changes in cell culture experiments are attributable solely to the actions of the peptide rather than artifacts of bacterial contamination.
Handling, Solubilization, and Storage Protocols for Laboratory Use
Humanin is supplied as a sterile, lyophilized (freeze-dried) powder to maximize long-term stability. Upon receipt, lyophilized vials should be stored immediately at -20°C or -80°C in a desiccated environment to prevent moisture absorption and peptide degradation.
For reconstitution in laboratory settings, researchers should allow the vial to equilibrate to room temperature prior to opening. Humanin contains hydrophobic residues; therefore, initial solubilization protocols may require dissolving the peptide in sterile, low-molarity acetic acid or sterile, endotoxin-free water before diluting into phosphate-buffered saline (PBS) or culture media. Aliquoting reconstituted solutions into single-use volumes is highly recommended to avoid repeated freeze-thaw cycles, which can cause aggregation or cleavage of the peptide backbone.
Procurement, Volume Fulfillment, and Shipping Logistics
PX1 Research maintains a streamlined supply chain designed to support high-throughput institutional studies and long-term academic projects. Whether ordering single units for initial feasibility assays or setting up bulk peptide procurement for multi-phase rodent trials, our logistics infrastructure guarantees rapid delivery and uninterrupted product availability.
Orders placed Monday through Friday before our daily cutoff time ship same-day directly from our centralized distribution facilities in California and Arizona. Material is packaged using temperature-controlled cold packs and protective insulation to ensure structural integrity during transit. Domestic orders arrive rapidly, preventing supply chain disruptions in sensitive, time-dependent laboratory workflows.
Frequently Asked Questions
What is the purity standard of Humanin supplied by PX1 Research?
PX1 Research guarantees a minimum purity of 98% for Humanin, as verified by lot-specific High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) testing.
Are Certificates of Analysis (COAs) provided with every order?
Yes. Every batch of Humanin comes with a accessible, lot-specific COA generated by an independent ISO 17025 accredited laboratory, detailing HPLC chromatograms, MS spectra, and endotoxin levels.
How does PX1 test for endotoxin contamination in Humanin?
All Humanin lots are subjected to quantitative Limulus Amebocyte Lysate (LAL) testing to ensure endotoxin levels remain below strictly defined laboratory limits (<0.01 EU/mg).
Where is PX1 Research Humanin synthesized and shipped from?
Humanin is synthesized in GMP-compliant facilities within the United States. All orders are packed and shipped same-day (Monday–Friday) from our domestic fulfillment centers located in California and Arizona.
What is the recommended storage procedure for lyophilized Humanin?
Lyophilized Humanin powder should be stored at -20°C or -80°C in a dry environment protected from light. Reconstituted solutions should be aliquoted and frozen to minimize freeze-thaw degradation.
How does Humanin differ mechanistically from MOTS-c?
While both are mitochondrial-derived peptides, Humanin acts primarily as an anti-apoptotic factor through membrane receptors (CNTFR/WSX-1/gp130) and intracellular BAX binding. MOTS-c acts primarily as a metabolic regulator targeting nuclear gene expression and purine pathways.
Can PX1 Research fulfill bulk or institutional orders for Humanin?
Yes. PX1 Research supports institutional procurement and high-volume laboratory requests. Institutional buyers can apply for lab accounts via our wholesale portal for custom quantities.
Is Humanin intended for human administration or therapeutic use?
No. Humanin provided by PX1 Research is strictly designated as a research compound intended solely for in vitro and preclinical laboratory investigation. It is not for human or veterinary consumption, therapy, or clinical application.
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All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.