To purchase MOTS-c for laboratory research, investigators require verified sequence purity, full analytical documentation, and batch-to-batch consistency. PX1 Research provides USA-manufactured, synthetic MOTS-c verified by RP-HPLC and mass spectrometry, ensuring accredited academic and private institutions receive research-grade compounds for metabolic and mitochondrial studies.
To purchase MOTS-c for laboratory research, investigators require verified sequence purity, full analytical documentation, and batch-to-batch consistency. PX1 Research provides USA-manufactured, synthetic MOTS-c verified by RP-HPLC and mass spectrometry, ensuring accredited academic and private institutions receive research-grade compounds for metabolic and mitochondrial studies.
MOTS-c (mitochondrial open reading frame of the 12S rRNA type-c) is a 16-amino-acid peptide derived from the mitochondrial genome. Unlike nuclear-encoded peptides, MOTS-c belongs to a unique class of mitochondrial-derived peptides (MDPs) that function as signaling molecules during metabolic stress. In preclinical models, MOTS-c translocates to the nucleus in response to cellular stress, where it regulates nuclear gene expression involved in metabolic homeostasis, folate metabolism, and glucose oxidation.
Primary research efforts focus on the capacity of MOTS-c to activate 5'-AMP-activated protein kinase (AMPK) independent of the upstream kinase LKB1. Researchers studying metabolic dysfunction, insulin resistance, and physical capacity utilize MOTS-c in both in vitro cell lines and rodent assays. Because its physiological effects rely heavily on structural stability and correct peptide folding, sourcing high-purity material with documented analytical validation is essential for reproducible empirical outcomes.
When evaluating the **mots c cost** across vendors, research departments must look beyond the initial listing fee to examine the underlying quality control infrastructure. The primary driver of **mots c peptide cost** is the rigors of chemical synthesis and purification. Synthesizing short mitochondrial peptides using Solid-Phase Peptide Synthesis (SPPS) requires precise coupling steps, followed by multiple rounds of preparative Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) to remove truncated or modified sequences.
Inexpensive peptide preparations frequently omit thorough purification steps or fail to perform comprehensive mass spectrometry validation. This results in crude yields contaminated with counter-ions, residual solvents, or side-product peptides that can alter cellular assays and skew experimental data. When institutions purchase research peptides from PX1 Research, the cost reflects rigorous synthesis in GMP-compliant facilities, full lot-specific analytical verification, and storage under nitrogen-blanketed environments to prevent oxidation.
A comprehensive analysis of **mots-c price** requires balancing cost per milligram against analytical transparency and structural assurance. Lower-tier suppliers often market raw chemical powders without batch traceability or verified endotoxin testing. For cell culture experiments or animal model administration, unverified preparations introduce high risk, including false-positive inflammatory responses triggered by bacterial lipopolysaccharides (LPS).
PX1 Research establishes clear value metrics for laboratory procurement. Every batch of MOTS-c includes lot-specific documentation that details pure peptide content, net peptide weight, and safety parameter testing. Laboratories managing continuous research pipelines can lower overall expenditure by utilizing wholesale lab purchasing accounts, securing bulk pricing structures without sacrificing the analytical rigor demanded by peer-reviewed publications.
Reliable empirical data requires strict physical characterization. A complete **mots-c coa hplc** document provides definitive proof of purity, identity, and safety. At PX1 Research, every lot of MOTS-c undergoes dual analytical validation through an independent ISO 17025 accredited testing laboratory.
The analytical testing regimen includes two core procedures:
1. **mots-c hplc Analysis**: Reverse-Phase High-Performance Liquid Chromatography separates the target peptide from synthesis impurities. Standard specifications require a single sharp chromatographic peak representing greater than 99% purity by peak area integration.
2. **LC-MS Mass Spectrometry**: Electrospray ionization mass spectrometry (ESI-MS) confirms the exact molecular mass of MOTS-c (2174.6 g/mol), verifying sequence integrity and confirming the absence of erroneous amino acid substitutions.
In addition to structural validation, PX1 Research measures residual endotoxins via the Limulus Amebocyte Lysate (LAL) assay, guaranteeing levels fall strictly below <0.5 EU/mg. Investigators can inspect these results directly through our public research portal before placing an order.
To maintain consistency across multi-week animal studies or automated in vitro screens, research procurement teams should evaluate vendor standards against the following core criteria:
• **Purity Verification**: RP-HPLC analytical profiles exceeding 99% purity.
• **Mass Identification**: High-resolution LC-MS spectra verifying molecular weight.
• **Endotoxin Control**: Certified LAL testing under 0.5 EU/mg for sensitive bioassays.
• **Domestic Synthesis & Storage**: USA-manufactured under climate-controlled conditions to preserve integrity.
• **Batch Traceability**: Accessible Certificate of Analysis (COA) linked to the exact vial lot number.
• **Expedited Logistics**: Same-day dispatch M–F from dual fulfillment hubs in California and Arizona to prevent temperature degradation during transport.
Ordering from qualified suppliers eliminates chemical variability, ensuring that observed physiological changes in cell culture or animal subjects stem strictly from the target compound.
MOTS-c belongs to an expanding class of signaling molecules investigated for mitochondrial protection, energy regulation, and metabolic enhancement. To select the appropriate molecule for a given study, researchers frequently compare MOTS-c with complementary agents targeting energy pathways.
For example, while MOTS-c operates as a nuclear-translocating peptide derived from mitochondrial DNA, the cardiolipin-targeted tetrapeptide SS-31 operates directly within the inner mitochondrial membrane to optimize electron transport chain efficiency and reduce reactive oxygen species (ROS). Similarly, humanin represents another major mitochondrial-derived peptide involved in cytoprotective and anti-apoptotic signaling. Non-peptidic metabolic activators such as AICAR are frequently evaluated alongside MOTS-c in comparative studies assessing direct versus indirect AMPK activation. Understanding these distinctions allows investigators to construct targeted multi-compound comparative models.
In preclinical rodent models, MOTS-c administration has demonstrated significant effects on systemic metabolic regulation. Studies indicate that MOTS-c acts primarily on skeletal muscle tissue, enhancing glucose uptake by stimulating GLUT4 translocation independently of traditional insulin receptor pathways. This mechanism relies on the phosphorylation of AMPK and downstream target proteins involved in fatty acid oxidation.
Furthermore, research shows that MOTS-c modulates the folate cycle, leading to alterations in de novo purine biosynthesis. During metabolic stress or physical exercise simulations, cellular MOTS-c levels rise, driving adaptive nuclear gene expression. In rodent fitness assays, treatment with synthetic MOTS-c maintained metabolic flexibility and exercise capacity in aging animals, making it a pivotal subject in longevity and metabolic disease research.
Synthetic MOTS-c is supplied as a lyophilized (freeze-dried) sterile powder to ensure long-term stability. Proper laboratory handling is essential to maintain structural integrity and prevent premature peptide degradation:
• **Lyophilized Powder Storage**: Upon receipt, store unopened vials at -20°C for short-term preservation or -80°C for long-term storage. Keep protected from direct light exposure.
• **Reconstitution Protocol**: Allow the vial to equilibrate to room temperature inside a laminar flow biosafety cabinet before opening. Reconstitute using sterile, preservative-free Water for Injection (WFI) or sterile bacteriostatic water, depending on assay timelines.
• **Solubilization Guidance**: Gently swirl or invert the vial to dissolve the cake. Avoid vigorous vortexing or rapid agitation, which can induce mechanical shear and peptide aggregation.
• **Solution Stability**: Reconstituted MOTS-c solutions should be aliquoted into single-use polypropylene tubes to prevent repeated freeze-thaw cycles. Store aliquots at -80°C for up to 90 days or maintain at 4°C for immediate use within 48 to 72 hours.
PX1 Research is dedicated to supporting academic institutions, biotechnology organizations, and contract research organizations (CROs) with dependable, high-purity research compounds. When you **purchase mots c** from PX1, your order is processed in our state-of-the-art US facilities and backed by our commitment to scientific transparency.
We maintain fully inventory-managed warehouses in California and Arizona, enabling same-day shipping for orders placed before cutoff times Monday through Friday. Whether conducting exploratory in vitro screens or scaling up to preclinical animal trials, explore our complete catalog of mitochondrial research peptides to equip your facility with verified laboratory reagents.
Where can accredited laboratories purchase mots c?
Accredited laboratories can purchase MOTS-c directly from PX1 Research. We provide high-purity, US-manufactured synthetic MOTS-c strictly for laboratory research use, accompanied by complete batch-specific analytical documentation.
How is the mots c cost determined for research applications?
The mots c cost is determined by the solid-phase synthesis complexity, multi-step RP-HPLC purification requirements, and comprehensive analytical testing (HPLC, mass spectrometry, and LAL endotoxin testing) necessary to guarantee >99% purity.
What factors influence the mots c peptide cost per milligram?
Key factors influencing mots c peptide cost include synthesis scale, purity grade, strict endotoxin thresholds (<0.5 EU/mg), third-party ISO 17025 verification, and temperature-controlled domestic fulfillment infrastructure.
Why is a mots-c coa hplc analysis critical before purchase?
A mots-c coa hplc analysis is critical because it verifies peptide purity, ensures the absence of truncated synthesis side-products, and provides empirical proof that the chemical compound meets rigorous laboratory quality standards.
What does mots-c hplc testing measure?
mots-c hplc testing measures the relative area percentage of the primary MOTS-c peak compared to potential impurities using Reverse-Phase High-Performance Liquid Chromatography, confirming structural uniformity and high purity.
How does mots-c price correlate with analytical purity?
A lower mots-c price from unverified vendors often indicates raw, unpurified preparations that lack mass spectrometry identity confirmation or endotoxin testing. Premium pricing reflects rigorous purification, batch stability, and full COA verification.
What is the recommended reconstitution solvent for MOTS-c in cell studies?
MOTS-c is typically reconstituted using sterile Water for Injection (WFI) or sterile phosphate-buffered saline (PBS, pH 7.4) under laminar flow conditions for immediate cell culture assays.
How should reconstituted MOTS-c be stored in the lab?
Reconstituted MOTS-c should be divided into single-use aliquots and stored at -80°C to minimize degradation and avoid freeze-thaw stress. Short-term storage at 4°C is acceptable for up to 72 hours.
What is the molecular weight and sequence of MOTS-c?
MOTS-c has a molecular weight of approximately 2174.6 g/mol and consists of a 16-amino-acid sequence: Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg.
Can institutions purchase MOTS-c in bulk through wholesale accounts?
Yes, university departments, contract research organizations, and institutional procurement officers can apply for PX1 Research wholesale accounts to receive volume pricing on continuous research orders.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.