Alpha Klotho LR is a specialized recombinant research peptide derived from the soluble domain of the anti-aging Klotho protein, engineered for high-precision laboratory assays. Formulated exclusively for in vitro and preclinical research, this compound allows investigators to probe FGFR co-receptor interactions, oxidative stress responses, and Wnt pathway modulation. PX1 Research supplies USA-manufactured Alpha Klotho LR verified through lot-specific RP-HPLC and mass spectrometry to ensure strict academic standards.
Alpha Klotho LR is a specialized recombinant research peptide derived from the soluble domain of the anti-aging Klotho protein, engineered for high-precision laboratory assays. Formulated exclusively for in vitro and preclinical research, this compound allows investigators to probe FGFR co-receptor interactions, oxidative stress responses, and Wnt pathway modulation. PX1 Research supplies USA-manufactured Alpha Klotho LR verified through lot-specific RP-HPLC and mass spectrometry to ensure strict academic standards.
Alpha Klotho LR is a specialized recombinant research peptide derived from the soluble extracellular domain of the transmembrane Klotho protein. Designed specifically for in vitro assays and controlled animal models, alpha klotho lr serves as a primary molecular tool for investigating fibroblast growth factor (FGF) signaling, phosphate homeostasis, cellular senescence pathways, and Wnt signaling inhibition in preclinical research settings.
Researchers utilize alpha klotho lr to isolate the systemic, endocrine actions of Klotho from its membrane-bound functions. In biological systems, soluble Klotho variants act as circulating humoral factors that modulate multi-organ signaling networks, particularly within renal, vascular, and central nervous system tissue models.
The structural architecture of alphaklotholr comprises tandem internal repeats known as the KL1 and KL2 domains. These regions exhibit sequence homology to family 1 glycosidases, although recombinant variants are structurally optimized to preserve specific binding interactions while maximizing stability in cell culture media and aqueous buffers.
Endogenous Klotho is expressed primarily as a single-pass transmembrane protein in renal distal convoluted tubules, choroid plexus, and parathyroid glands. Cleavage by membrane-bound metalloproteinases (such as ADAM10 and ADAM17) releases the soluble extracellular fragment into systemic circulation. Recombinant production of alpha klotho lr isolates this soluble functional domain, eliminating the transmembrane domain to allow precise dosing in liquid assays without membrane-anchoring artifacts.
Through molecular engineering, recombinant variants maintain the structural binding pockets necessary to complex with Fibroblast Growth Factor Receptors (FGFRs), particularly FGFR1c, FGFR3c, and FGFR4. Understanding these structural dynamics is essential for designing assays that measure receptor phosphorylation, downstream ERK signaling, or ion channel regulation.
In preclinical laboratory investigations, Alpha Klotho LR functions through two primary pathways: as an essential co-receptor for the endocrine hormone FGF23, and as an independent enzyme/ligand interacting with cell-surface signaling complexes. When complexed with FGFRs, Alpha Klotho LR dramatically increases receptor affinity for FGF23, initiating intracellular signal transduction cascades that regulate phosphate reabsorption and vitamin D biosynthesis in renal cell models.
Independent of FGF23, in vitro data indicate that Alpha Klotho LR exerts potent direct signaling effects. It directly binds to cell-surface Wnt ligands, sequestering them and blunting canonical Wnt/β-catenin signaling. Overactivation of Wnt signaling is widely associated with cellular senescence, tissue fibrosis, and stem cell depletion; thus, alpha klotho lr provides researchers with a targeted mechanism to suppress Wnt-driven fibrotic changes in vitro.
Additionally, preclinical studies suggest that Alpha Klotho LR enhances cellular defense against oxidative stress. Exposure of cell cultures to recombinant Klotho results in the activation of FOXO transcription factors and upregulates manganese superoxide dismutase (MnSOD), accelerating the clearance of reactive oxygen species (ROS) and extending cellular viability under oxidative challenge.
Academic and private research institutions deploy Alpha Klotho LR across several distinct fields of inquiry within the research library hub:
1. Renal Pathophysiological Models: Investigators utilize Alpha Klotho LR to explore renal protection mechanisms, mineral ion balance, and the mitigation of acute kidney injury (AKI) markers in tubular epithelial cell cultures.
2. Vascular and Endothelial Function: In vitro assays evaluate how Klotho exposure alters nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs), as well as its impact on vascular calcification under hyperphosphatemic conditions.
3. Neurobiology and Synaptic Plasticity: Rodent models and neuronal cell cultures are treated with Klotho variants to quantify changes in NMDA receptor subunit expression, synaptic density, and resistance to neurotoxic insults.
4. Cellular Senescence and Longevity Pathways: Laboratory teams use Alpha Klotho LR in conjunction with other longevity-focused peptides from our all peptides catalog to measure changes in senescence-associated beta-galactosidase (SA-β-gal) activity and telomere integrity.
When designing protocols targeting cellular aging, mitochondrial repair, or metabolic homeostasis, laboratories frequently evaluate Alpha Klotho LR alongside complementary synthetic peptides and recombinant proteins.
While alpha klotho lr functions predominantly as an extracellular co-receptor ligand modulating Wnt and FGF23 pathways, other research peptides operate through distinct intracellular mechanisms. For instance, epitalon is a synthetic tetrapeptide studied for its capacity to upregulate telomerase activity and restore pineal gland function. In contrast, ss-31 selectively targets cardiolipin within the inner mitochondrial membrane to optimize electron transport chain efficiency and reduce ROS production. Meanwhile, mitochondrial-derived peptides like mots-c act as metabolic regulators targeting AMP-activated protein kinase (AMPK) pathways.
Combining these agents in multi-arm in vitro studies allows researchers to cross-examine extracellular signaling cross-talk (Alpha Klotho LR) alongside direct mitochondrial stabilization (SS-31) and nuclear telomere regulation (Epitalon). Institutional buyers seeking bulk supply for comparative series can utilize our wholesale program to coordinate large-scale lot reservations.
To ensure reproducible data across demanding analytical procedures, PX1 Research enforces rigorous quality assurance metrics on every lot of Alpha Klotho LR. Recombinant proteins require strict physical and chemical validation to confirm structural integrity, correct molecular weight, and freedom from biological contaminants.
Every batch produced in our USA-based, GMP-compliant facilities undergoes comprehensive testing at an independent ISO 17025 accredited laboratory. Our verification standards include:
- Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC): Ensures peptide chemical purity exceeds 98.0%, eliminating truncated sequences or unreacted reagents.
- Electrospray Ionization Mass Spectrometry (ESI-MS): Confirms exact molecular mass and primary sequence fidelity.
- Limulus Amebocyte Lysate (LAL) Endotoxin Testing: Guarantees endotoxin content remains strictly below 0.01 EU/μg, ensuring compatibility with sensitive primary cell cultures.
- Amino Acid Analysis (AAA) & SDS-PAGE: Validates accurate protein quantification and conformational purity without aggregate contamination.
To maintain the biological activity of lyophilized Alpha Klotho LR, laboratory personnel must follow standard sterile handling procedures. Recombinant proteins are sensitive to mechanical shear stress, improper pH, and repeated freeze-thaw cycles.
Reconstitution Protocol:
1. Centrifugation: Centrifuge the vial briefly prior to opening to consolidate the lyophilized cake at the bottom of the container.
2. Reconstitution Medium: Reconstitute using sterile, endotoxin-free water or sterile phosphate-buffered saline (PBS, pH 7.4). For long-term storage or low-concentration working solutions, adding a carrier protein such as 0.1% purified Bovine Serum Albumin (BSA) is recommended to prevent non-specific binding to plasticware.
3. Dissolution: Gently swirl or invert the vial. Do not vortex vigorously, as physical agitation can cause protein denaturation or aggregation.
Storage Guidelines:
- Lyophilized Powder: Store at -20°C to -80°C upon receipt. Under these conditions, the protein remains stable for up to 24 months.
- Reconstituted Stock Solutions: Store in single-use aliquots at -80°C. Avoid repeated freeze-thaw cycles. Aliquots stored at 4°C should be utilized within 2 to 7 days depending on buffer composition and carrier protein presence.
In vitro experimental design requiring Alpha Klotho LR must account for biological half-life, receptor expression density, and buffer interaction. In cell culture models, working concentrations typically range between 10 pM and 100 nM, depending on whether the experiment measures immediate phosphorylation cascades (e.g., ERK1/2) or long-term transcriptional changes over 24–72 hours.
When designing serum-free media experiments, researchers should account for endogenous background signaling factors. Because Klotho interacts dynamically with calcium and phosphate transport proteins (such as TRPV5 and NaPi-2a), buffer ion concentrations should be tightly controlled and monitored throughout the experiment.
Control groups should include vehicle-only wells (matching buffer and carrier protein concentrations) and, where applicable, receptor-inhibited controls to confirm specific FGFR-mediated binding kinetics.
PX1 Research is dedicated to supporting the global scientific community by providing American-manufactured research reagents with full supply chain transparency. Every shipment originates from our dual distribution centers located in California and Arizona, ensuring rapid transit times across North America.
Orders placed Monday through Friday prior to cut-off times qualify for same-day dispatch. Every order includes a lot-specific Certificate of Analysis (COA) containing raw HPLC chromatograms and mass spectra. Principal investigators and laboratory procurement officers can review full documentation or establish dedicated institutional accounts via our wholesale portal.
What is alpha klotho lr in laboratory research?
Alpha klotho lr is a recombinant research protein corresponding to the functional extracellular domain of human Alpha Klotho. In laboratory settings, it is used to study cellular senescence, Wnt pathway signaling, FGF23 co-receptor activation, and mineral homeostasis in cell cultures and animal models.
How is alphaklotholr synthesized and quality tested?
Recombinant alphaklotholr is expressed in optimized cell systems and purified using chromatography techniques. PX1 Research validates every lot through RP-HPLC for purity (>98%), mass spectrometry for sequence verification, and LAL assays to confirm endotoxin levels remain below 0.01 EU/μg.
Is Alpha Klotho LR approved for human administration or clinical use?
No. Alpha Klotho LR is strictly a research peptide supplied for laboratory research use only. It is not intended, designed, or approved for human or animal therapeutic use, clinical administration, diagnosis, or personal consumption.
What is the purity level of PX1 Research Alpha Klotho LR?
PX1 Research guarantees a minimum purity of 98.0% for Alpha Klotho LR, verified via lot-specific Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC).
How should Alpha Klotho LR be reconstituted for in vitro assays?
Reconstitute the lyophilized powder in sterile endotoxin-free water or PBS (pH 7.4). It is recommended to include 0.1% BSA or HSA as a carrier protein if preparing low-concentration aliquots to prevent protein adsorption to plastic surfaces.
What are the recommended storage conditions for Alpha Klotho LR?
Lyophilized powder should be stored at -20°C or -80°C for long-term stability. Once reconstituted, divide the solution into single-use aliquots and store at -80°C, avoiding repeated freeze-thaw cycles.
What receptor complexes does Alpha Klotho LR target?
Alpha Klotho LR functions as a co-receptor for Fibroblast Growth Factor 23 (FGF23), binding primarily to FGFR1c, FGFR3c, and FGFR4 complexes. It also directly interacts with cell-surface Wnt ligands.
Are Certificates of Analysis (COA) provided with Alpha Klotho LR orders?
Yes. Every shipment includes or provides access to a lot-specific COA generated by an independent ISO 17025 accredited testing facility, showing exact HPLC chromatograms and mass spectrometry output.
How does Alpha Klotho LR differ from full-length membrane Klotho?
Full-length Klotho contains a transmembrane domain anchoring it to the cell membrane. Alpha Klotho LR represents the soluble, circulating extracellular fragment (KL1 + KL2 domains) that can act systemically or be added directly to cell culture media without membrane insertion.
Can academic labs order Alpha Klotho LR in bulk or wholesale quantities?
Yes. Institutional accounts, university departments, and private research facilities can request custom supply agreements and bulk reservation through the PX1 Research wholesale department.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.