PX1 Research provides USA-manufactured, research-grade Epithalon (Ala-Glu-Asp-Gly) verified by independent HPLC and mass spectrometry. Designed strictly for in vitro assays and animal models evaluating telomerase activation, pineal function, and circadian gene expression, our synthetic bioregulators set the industry standard for purity, low endotoxin levels, and lot-to-lot consistency.
PX1 Research provides USA-manufactured, research-grade Epithalon (Ala-Glu-Asp-Gly) verified by independent HPLC and mass spectrometry. Designed strictly for in vitro assays and animal models evaluating telomerase activation, pineal function, and circadian gene expression, our synthetic bioregulators set the industry standard for purity, low endotoxin levels, and lot-to-lot consistency.
Qualified institutions seeking Epithalon for sale in the USA require analytical precision, lot-specific verification, and reliable domestic distribution. PX1 Research supplies high-purity Epithalon (epitalon tetrapeptide) manufactured in domestic GMP-compliant facilities and tested by ISO 17025 accredited laboratories. Every lot includes a comprehensive Certificate of Analysis (COA) confirming identity, purity, and safety limits for in vitro and preclinical investigation.
Epithalon is a synthetic short-chain peptide composed of four amino acids (L-alanyl-L-alpha-glutamyl-L-alpha-aspartyl-glycine). Categorized as a synthetic pineal bioregulator, it has become a central focus in gerontological and cellular biology research. To maintain experimental integrity, investigators require access to reference-grade compounds that possess verified sequence fidelity and minimal residual impurities.
Epithalon (CAS Registry Number 307297-39-8) is a synthetic tetrapeptide modeled after Epithalamin, a natural peptide extract derived from the pineal gland. Its primary amino acid sequence is H-Ala-Glu-Asp-Gly-OH, representing a molecular formula of C17H28N4O9 and a molecular mass of approximately 432.42 g/mol. This compact structure facilitates rapid diffusion across cellular membranes in cell culture models.
Unlike complex proteins, short-chain bioregulators like Epithalon operate through sequence-specific interactions with nuclear DNA and chromatin structures. Preclinical research indicates that short peptide chains can penetrate the cell nucleus and bind directly to the promoter regions of specific genes, initiating targeted transcriptional responses without triggering off-target cell surface receptor cascades.
The primary biochemical target associated with Epithalon in empirical literature is the activation of the telomerase enzyme complex (specifically human telomerase reverse transcriptase, or hTERT, in human cell models). In vitro studies on somatic cell lines—which typically express minimal baseline telomerase activity—demonstrate that exposure to Epithalon induces telomerase catalytic subunit expression. This enzymatic re-activation permits the elongation of telomeric repeats at chromosome ends.
Beyond telomerase induction, Epithalon alters chromatin accessibility. In vitro epigenetic assays demonstrate that the tetrapeptide interacts with histones and promoter regions, facilitating de-condensation of heterochromatin. This structural shift allows transcription factors to access previously silenced genomic regions, modulating the expression of genes involved in DNA repair, protein synthesis, and free radical neutralization.
Researchers investigating genomic stability often reference our broader selection of research peptides to evaluate complementary pathways governing chromatin remodeling, cell cycle regulation, and cellular longevity.
Somatic cell aging is limited by the Hayflick limit, a state of replicative senescence driven by progressive telomere shortening during each cell division cycle. In vitro experiments using human fetal fibroblast cultures revealed that continuous administration of Epithalon extended the proliferative life span of cells significantly past their expected Hayflick boundary. The treated cultures preserved telomere lengths comparable to early-passage controls.
In rodent models, chronic administration of Epithalon yielded measurable reductions in marker parameters of age-associated degeneration. Studies examining aging mice and rats demonstrated decreased spontaneous tumor incidence, restored immune cell proliferation kinetics, and attenuated lipid peroxidation levels in blood plasma. These findings position Epithalon as a primary benchmark compound for exploring non-genetic interventions in telomere maintenance.
As a biomimetic of pineal gland peptides, Epithalon demonstrates pronounced regulatory effects on melatonin synthesis and circadian architecture in preclinical research. Aging animals characteristically exhibit a nocturnal decline in endogenously synthesized melatonin. In senescent rodent and non-human primate models, administration of Epithalon restored peak nocturnal melatonin secretion to levels observed in younger control cohorts.
This neuroendocrine reset correlates with stabilized circadian gene expressions, such as CLOCK and BMAL1, within hypothalamic tissue preparations. Investigating how pineal bioregulators harmonize endocrine signals provides vital insights into neuroprotection, metabolic rate regulation, and sleep-wake architecture degradation during biological aging. Researchers can review detailed mechanistic breakdowns in our dedicated bioregulators overview.
Acquiring analytical-grade peptides requires verifying that the supplier enforces strict purity, safety, and traceability metrics. When evaluating vendors offering Epithalon for sale, research institutions must look beyond basic claims and inspect lot-specific documentation verified by independent laboratories.
PX1 Research maintains rigorous quality control parameters across every production run:
• High-Performance Liquid Chromatography (RP-HPLC): Ensures chemical purity exceeds 98.0%, confirming the absence of peptide truncations or incomplete synthetic sequences.
• Electrospray Ionization Mass Spectrometry (ESI-MS): Verifies exact monoisotopic mass and structural sequence integrity.
• Endotoxin Quantitation (LAL Assay): Guarantees bacterial endotoxin levels remain below stringent thresholds (< 0.05 EU/mg), eliminating confounding inflammatory variables in cell cultures.
• Domestic Synthesis and Storage: Formulated in US-based GMP-compliant facilities and shipped directly from centralized fulfillment nodes in California and Arizona.
Every vial sold by PX1 Research includes batch-traceable COAs accessible directly from our online database, ensuring absolute transparency for research compliance.
Epithalon belongs to a distinct class of peptide bioregulators that target specific tissue types and biological pathways. Understanding how Epithalon compares to related compounds helps researchers design precise controlled studies.
While Epithalon specifically targets pineal function, telomerase activation, and neuroendocrine homeostasis, other short-chain peptides influence distinct physiological systems. For instance, Thymalin acts primarily on thymic tissue to modulate T-cell differentiation and immune responsiveness. Similarly, Pinealon is a tripeptide bioregulator designed specifically for central nervous system models, mitigating oxidative stress in cortical neurons. For studies focused on mitochondrial energy production rather than nuclear chromatin dynamics, researchers frequently contrast bioregulators with mitochondrial-targeted peptides such as MOTS-c or SS-31. Choosing the appropriate compound depends entirely on whether the experimental focus is nuclear DNA repair, organ-specific peptide signaling, or bioenergetic flux.
To ensure reagent stability and reproducibility across experimental trials, researchers must adhere to validated reconstitution and storage protocols. Epithalon is supplied as a lyophilized (freeze-dried) sterile powder in sealed glass vials.
For reconstituted assays, sterile bacteriostatic water or sterile normal saline (0.9% NaCl) should be added slowly along the inner glass wall of the vial to minimize shear force degradation. The vial should be gently swirled—never vigorously shaken—until full dissolution occurs. For long-term preservation of lyophilized powder, vials must be stored at -20°C or -80°C protected from light. Reconstituted solutions should be aliquoted into single-use microtubes to prevent freeze-thaw degradation cycles and stored at -20°C for up to 30 days. Further details are detailed in our comprehensive peptide reconstitution guide.
Securing consistent peptide supplies for ongoing study schedules requires a reliable logistical pipeline. PX1 Research operates state-of-the-art dispatch centers in California and Arizona, facilitating same-day shipping on all domestic orders placed before 12:00 PM PST Monday through Friday.
Academic laboratories, commercial biotechnology entities, and clinical researchers requiring large volume procurement can establish institutional accounts via our wholesale portal. Bulk orders undergo identical analytical verification standards, ensuring lot-to-lot consistency across multi-phase experimental designs.
What is the certified purity level of PX1 Research Epithalon?
Every lot of Epithalon supplied by PX1 Research undergoes rigorous RP-HPLC analysis to ensure chemical purity exceeds 98.0%. Exact purity specifications and mass spectrometry mass verification data are provided on the lot-specific Certificate of Analysis.
How is Epithalon verified for laboratory endotoxin limits?
PX1 Research utilizes standard Limulus Amebocyte Lysate (LAL) testing to quantify endotoxin levels in every batch. Our research-grade peptides are guaranteed to fall below 0.05 EU/mg, preventing cell culture contamination or pyrogenic reactions in preclinical models.
What is the recommended storage temperature for lyophilized Epithalon?
Lyophilized Epithalon should be stored at -20°C upon receipt for short-to-medium-term stability, or at -80°C for long-term storage up to two years. Reagents should be kept away from direct light exposure.
How fast does PX1 Research ship Epithalon within the United States?
Orders placed before 12:00 PM PST, Monday through Friday, ship the same day from our domestic facilities in California or Arizona. Express shipping options are available at checkout to preserve temperature integrity during transit.
Is Epithalon approved for human clinical use or therapeutic treatment?
No. Epithalon provided by PX1 Research is strictly manufactured and labeled for laboratory research use only (in vitro and animal preclinical research). It is not intended for human consumption, clinical diagnostic, or therapeutic use.
What solvent is best suited for reconstituting Epithalon for cell assays?
For most cellular assays, sterile reconstituted solution in bacteriostatic water, sterile phosphate-buffered saline (PBS), or 0.9% sodium chloride is recommended, depending on the buffer tolerance of the target biological culture.
Can academic labs request custom batch testing or volume discounts?
Yes. Principal investigators and institutional buyers can contact our team through the wholesale portal to arrange volume-based pricing, custom aliquoting, or specialized third-party analytical reporting.
All products are sold strictly for laboratory and research use only. Not for human or veterinary use, diagnosis, treatment or consumption. Statements have not been evaluated by the FDA.